FastQCFastQC Report
Thu 26 May 2016
SRR936066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1110540
Sequences flagged as poor quality0
Sequence length125
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168371.5161092801700073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130981.179426225079691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100420.9042447818178544No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56780.5112827993588704No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32730.29472148684423793No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31750.2858969510328309No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26500.23862265204315017No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.21989302501485763No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT21110.19008770508041134No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.1746897905523439No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16770.15100761791560863No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT15280.1375907216309183RNA PCR Primer, Index 46 (95% over 21bp)
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA12640.11381850271039314No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG11850.10670484629099358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC11810.1063446611558341No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13400.055.968243100-101
TTCGGTA800.055.79161598-99
ACGTATC3500.054.419914100-101
CCGTACA3850.053.337696100-101
CCCGGTA2700.052.89871298-99
TCGAATA1100.051.405174100-101
CCCGTAC2550.051.34287398-99
CGGTATC12400.050.881523100-101
CGAATCG1300.050.3532496-97
CCGGTAT5000.049.998295100-101
AACGGTA1450.049.2505398-99
CCGTATC5450.048.600353100-101
CGAGGTA1850.048.2522198-99
CGTACAT11600.048.23317100-101
GCGTACA2050.047.907776100-101
TGAGTAC11100.047.72467100-101
TGTATCA15750.047.05055100-101
TTTACGC3100.046.07307498-99
CGAGCGT1050.045.33971896-97
AGGTATC18300.044.885277100-101
Produced by FastQC (version 0.11.5)