FastQCFastQC Report
Thu 26 May 2016
SRR936057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCTTATTGATACCCCAATTCTCATCCATGTCCTTCCAGCCCCAGGCC14.545454545454546No Hit
CCAAGCAGAGCAAAATAAAGTAACAACAGTGACTGAGAAGTTAATTGAGG14.545454545454546No Hit
CAGAGGGGGGAGCCCCTTGGAAGGTACAGAGGCCTCCTAGTCGTGCTTGA14.545454545454546No Hit
TGTTTCATGTGGTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGGCATCGG14.545454545454546No Hit
AGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCA14.545454545454546No Hit
TGGGCTCTGTGTGTGCATCTCTAGCCTACCCCCTCCAGAGAAGCTTGATA14.545454545454546No Hit
AAAGACAACATATCAACTGGTGACCAAGGTCAGAACTGTCAGACGTCTCC14.545454545454546No Hit
AAGGGCAGTACTGGGAGTTAAAGTGCCTGTTACTTGAACTGGTAGATGAC14.545454545454546No Hit
TGGAAAAAACATCCACCTGCACGCCCACAGCCTGGTGGGCAAGCACTGTG14.545454545454546No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG14.545454545454546No Hit
GTATGAGGCAGGGACACAGAGTTGAGGACCAGCCTGTGAAGTTTGACAAT14.545454545454546No Hit
TTTTTTTTTTTTTTTTTTTTTCTCTGCAATATTTTTTTCTTGTTTGCATG14.545454545454546No Hit
TATAATTTTTAAAAATTAAGAACACGGTTTATTCCTCTGAGCTTGGTGCA14.545454545454546No Hit
CGAGAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAACCTTTCTTTTTAAA14.545454545454546No Hit
CTTTGACGTTTCAGAGGTTCTCAGAGATACAAACTGATCTGTGCAAGTCT14.545454545454546No Hit
CTACATAGCATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAA14.545454545454546No Hit
TGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACCTTCGGAAGC14.545454545454546No Hit
AGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAAGTAAA14.545454545454546No Hit
CTAAATTTGTTGAAGCATACGATACTGATATTACTTCTCGTTTTGAAGCA14.545454545454546No Hit
GGGTAGTCATAACTATTACGTTTTGTTATGTATTAATCATTTCAGTTGAA14.545454545454546No Hit
AATCTCCATACATGGCCTCCATTTTCAACTGTTTTCTTGGTCACAGAGCT14.545454545454546No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC14.545454545454546No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)