FastQCFastQC Report
Thu 26 May 2016
SRR936041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3665425
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47620.12991672179897285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG3800.029.89449756
CTCGCGC4250.029.2306736
TCGCGCT4700.026.43451537
CGGTTAC3550.025.9934064
CGCGGAA4100.025.97433959
CGATCGT5100.025.0538331
TCGCGGA4850.022.6889958
CCTCGCG5650.022.61650835
CAATTCG4750.022.42301454
CGCGCTT5700.021.79598838
AATTCGC4900.021.73422255
CGCTCCG6300.020.84704625
CGTTCGG1459.908699E-619.58284211
GCGCCCT7100.018.49682231
GACGCTC6550.018.42733223
ACTCGAG6950.018.38558214
ATCGTTC6650.018.1455253
ACGATCG3800.017.7491359
AACTCGA7400.017.26777513
CGCGAGG4150.017.1066625
Produced by FastQC (version 0.11.5)