##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936041_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3665425 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.49271148638971 39.0 36.0 41.0 30.0 41.0 2 36.352988807573475 39.0 36.0 41.0 29.0 41.0 3 36.264422270268796 39.0 36.0 41.0 29.0 41.0 4 36.14307699652837 39.0 36.0 41.0 29.0 41.0 5 36.07680200795269 39.0 36.0 41.0 29.0 41.0 6 35.9863090910331 39.0 35.0 40.0 28.0 41.0 7 35.83227511134452 39.0 35.0 40.0 27.0 41.0 8 35.6679413710552 39.0 35.0 40.0 27.0 41.0 9 35.58343166208557 39.0 35.0 40.0 27.0 41.0 10-11 35.8101667610168 39.0 35.0 40.0 28.0 41.0 12-13 36.007513317009625 39.0 35.0 41.0 29.0 41.0 14-15 35.964584870785785 39.0 35.0 41.0 28.0 41.0 16-17 35.811721560255634 39.0 35.0 41.0 27.0 41.0 18-19 35.67832979804525 39.0 35.0 41.0 27.0 41.0 20-21 35.507502131403584 39.0 35.0 41.0 26.5 41.0 22-23 35.28835332874087 39.0 35.0 41.0 26.0 41.0 24-25 35.09394190305353 39.0 34.5 41.0 25.5 41.0 26-27 34.17637286262848 38.0 33.5 40.0 23.5 40.5 28-29 34.21988473369391 38.0 33.5 40.0 24.0 40.5 30-31 34.5951646807669 38.0 34.0 40.0 24.0 41.0 32-33 34.43710374649597 38.0 34.0 40.0 23.0 41.0 34-35 34.166870281072455 38.0 33.5 40.0 22.5 41.0 36-37 33.875823813063974 38.0 33.0 40.0 22.0 41.0 38-39 33.595900611798086 37.0 33.0 40.0 21.5 41.0 40-41 33.217608872095326 37.0 32.5 40.0 20.0 41.0 42-43 32.854866761698844 36.0 32.0 39.0 19.0 41.0 44-45 32.48615603920419 36.0 32.0 39.0 18.0 41.0 46-47 32.091617888785066 35.0 31.5 39.0 15.5 40.0 48-49 31.623013020318243 35.0 31.0 37.5 12.0 40.0 50-51 31.17761419207868 35.0 31.0 37.0 7.5 39.0 52-53 30.719075686994003 35.0 30.5 36.5 6.5 39.0 54-55 30.252875451004996 34.5 30.0 36.0 2.0 38.5 56-57 29.792819932204317 34.0 29.5 36.0 2.0 37.0 58-59 29.36619314267786 34.0 29.0 35.0 2.0 37.0 60-61 29.01798086170089 34.0 29.0 35.0 2.0 36.5 62-63 28.668430536704477 34.0 29.0 35.0 2.0 36.0 64-65 28.298007598027514 34.0 29.0 35.0 2.0 36.0 66-67 27.920662406132983 34.0 27.5 35.0 2.0 35.0 68-69 27.55377834766773 34.0 27.0 35.0 2.0 35.0 70-71 27.130725904908708 33.0 26.0 35.0 2.0 35.0 72-73 26.64342252262698 33.0 25.0 35.0 2.0 35.0 74-75 26.05038965467852 33.0 24.5 35.0 2.0 35.0 76-77 24.333893750383652 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9423938669569409 1101 2 0.8343658336917841 1101 3 0.965812905761311 1101 4 0.9957130552436197 1101 5 1.0162461224473205 1101 6 0.9715719229542117 1101 7 1.0840036778076225 1101 8 1.1028552824591742 1101 9 1.1730175640122908 1101 10-11 1.0894355006854326 1101 12-13 1.1268864468014925 1101 14-15 1.074274337747248 1101 16-17 1.0825547360439955 1101 18-19 1.1107431646275145 1101 20-21 1.0134784821195737 1101 22-23 1.0419956508711223 1101 24-25 1.1813361427339828 1101 26-27 1.0039857123605813 1101 28-29 1.0885690806233228 1101 30-31 1.149897997370445 1101 32-33 1.1549093737587413 1101 34-35 1.036533432256057 1101 36-37 1.0656917558613017 1101 38-39 1.1344139150344432 1101 40-41 1.0934128629230173 1101 42-43 1.1446270885411067 1101 44-45 1.0691046508428101 1101 46-47 1.0118326825808381 1101 48-49 1.2338300222383154 1101 50-51 1.1341459999031578 1101 52-53 1.1706289949854884 1101 54-55 1.1124362888159887 1101 56-57 1.1716492024487302 1101 58-59 1.199532346261126 1101 60-61 1.2099846736797666 1101 62-63 1.0946570595028895 1101 64-65 0.9797914682625404 1101 66-67 1.1867263876441179 1101 68-69 1.139961094914561 1101 70-71 1.1375712663852866 1101 72-73 1.2049129392446751 1101 74-75 1.1427285275153558 1101 76-77 1.304502770938246 1102 1 0.787051956962344 1102 2 0.9121940129642709 1102 3 0.8943261197257755 1102 4 0.8782680909501153 1102 5 0.9108529268866192 1102 6 0.9468852942192996 1102 7 0.8727095205619761 1102 8 0.9089200932997556 1102 9 0.967806251532707 1102 10-11 0.852811736552205 1102 12-13 0.9576915809905273 1102 14-15 0.9517363587751788 1102 16-17 0.895315426361627 1102 18-19 1.0533771712839552 1102 20-21 1.0389696393886467 1102 22-23 0.9780752247615254 1102 24-25 0.954051805919292 1102 26-27 0.865429010000426 1102 28-29 0.9234029649272841 1102 30-31 1.0225892562266523 1102 32-33 0.9065351414729577 1102 34-35 1.0340873584435215 1102 36-37 0.9787418117953095 1102 38-39 1.0244645661049603 1102 40-41 1.1941460785636409 1102 42-43 1.1988204766898818 1102 44-45 1.1162892378252067 1102 46-47 0.9758627203486014 1102 48-49 1.1131828061979974 1102 50-51 1.1054150555531699 1102 52-53 1.012534154520413 1102 54-55 0.9731323576238147 1102 56-57 1.0540133381113534 1102 58-59 1.1956865225823599 1102 60-61 1.154624636451505 1102 62-63 1.1517261631981306 1102 64-65 1.079290047094208 1102 66-67 1.1412002686500706 1102 68-69 1.1723679996516623 1102 70-71 1.219452328659841 1102 72-73 1.1776236062013332 1102 74-75 1.198074101976463 1102 76-77 1.2948745548175111 1103 1 0.8131429279293414 1103 2 0.7073183049976706 1103 3 0.7979181013175847 1103 4 0.8000133836909029 1103 5 1.023878801083434 1103 6 0.877237621653947 1103 7 0.9538918643749241 1103 8 0.861772561722276 1103 9 0.6841540541456155 1103 10-11 0.7746159224272375 1103 12-13 0.9274061995112035 1103 14-15 0.9047940428854488 1103 16-17 0.9285997427685757 1103 18-19 0.9859433047595871 1103 20-21 0.9698104707649406 1103 22-23 0.9246812528104087 1103 24-25 0.8662573847037365 1103 26-27 0.6486095542491981 1103 28-29 0.6633549748555438 1103 30-31 0.8529241909225931 1103 32-33 0.9063930951283368 1103 34-35 0.9915164288397449 1103 36-37 0.9813844242083718 1103 38-39 0.8558176619023712 1103 40-41 0.9588104689887942 1103 42-43 1.036489914057647 1103 44-45 0.957085981064516 1103 46-47 0.6295008087866876 1103 48-49 0.7698221158536 1103 50-51 0.9511011990952589 1103 52-53 0.833396270408894 1103 54-55 0.8191949769025193 1103 56-57 0.9542563283888619 1103 58-59 0.7535018828262849 1103 60-61 0.7408313140099416 1103 62-63 0.6896579565656218 1103 64-65 0.7643536982517567 1103 66-67 0.9152199033077189 1103 68-69 0.8152982070113808 1103 70-71 0.8089863624559506 1103 72-73 0.7276643529679703 1103 74-75 0.6964692111451107 1103 76-77 0.8525075199339796 1104 1 0.728180791344812 1104 2 0.7626619986617627 1104 3 0.5755702167343273 1104 4 0.43629124616327175 1104 5 0.7017942138437405 1104 6 0.4870796516085747 1104 7 0.7141492322609793 1104 8 0.7222306549552258 1104 9 0.813669726491085 1104 10-11 0.675641178471615 1104 12-13 0.8025092668934803 1104 14-15 0.729588564441265 1104 16-17 0.8258565075087319 1104 18-19 0.8256704527289855 1104 20-21 0.7492868224128699 1104 22-23 0.8062472365861666 1104 24-25 0.8465230013246767 1104 26-27 0.7385586388369418 1104 28-29 0.7790567753137481 1104 30-31 0.8601840140321286 1104 32-33 0.8951347759539701 1104 34-35 0.9275583420445983 1104 36-37 0.7653459940999667 1104 38-39 0.8604894089068367 1104 40-41 1.0087466679590449 1104 42-43 1.049839637842915 1104 44-45 1.0470945943289465 1104 46-47 0.7354894438259123 1104 48-49 0.9190069157745455 1104 50-51 0.9104800257148078 1104 52-53 0.8019982795686182 1104 54-55 0.8690165079232308 1104 56-57 0.8570500961377299 1104 58-59 0.8611375893973978 1104 60-61 0.7191963187730757 1104 62-63 0.8330444376365911 1104 64-65 0.7647119572118655 1104 66-67 0.7983193775658286 1104 68-69 0.7227163998110555 1104 70-71 0.7205298841891796 1104 72-73 0.5948813338239809 1104 74-75 0.5351107132342214 1104 76-77 0.5676215833999088 1105 1 0.035332959998484625 1105 2 0.25338440634252635 1105 3 0.3216633107033715 1105 4 0.2195531460379243 1105 5 0.15862779647395087 1105 6 -0.06384803396372973 1105 7 -0.03697301291030897 1105 8 0.24993600077729639 1105 9 0.08652082177683695 1105 10-11 0.23224077723950387 1105 12-13 0.36048519558573844 1105 14-15 -0.0345068578263934 1105 16-17 0.1215363137413803 1105 18-19 0.2604002577994464 1105 20-21 0.35766909028441063 1105 22-23 0.26892403194108994 1105 24-25 0.24179621466159062 1105 26-27 0.16671194970572856 1105 28-29 0.28408318474139804 1105 30-31 0.2933964719356581 1105 32-33 0.41415528475531005 1105 34-35 0.46151104635099927 1105 36-37 0.5193258231507301 1105 38-39 0.3540089995819855 1105 40-41 0.3654591541806056 1105 42-43 0.3247656017444669 1105 44-45 0.21563786247842387 1105 46-47 0.02954399155422749 1105 48-49 0.07318520838393283 1105 50-51 0.028337007222319244 1105 52-53 -0.03207787006974527 1105 54-55 -0.06279428933244091 1105 56-57 -0.06762152929428211 1105 58-59 -0.057142345624999535 1105 60-61 -0.17598125982910418 1105 62-63 -0.21764594120627478 1105 64-65 0.00767705466681079 1105 66-67 0.06703514016710344 1105 68-69 -0.2845157468167585 1105 70-71 -0.5952700870671954 1105 72-73 -0.6591469059632722 1105 74-75 -0.7576116184953001 1105 76-77 -0.5456556933287935 1106 1 0.05651273672570056 1106 2 0.1821334739210485 1106 3 -0.12888576293649834 1106 4 0.022800807937088052 1106 5 -0.12674930915290616 1106 6 0.0046278752633099884 1106 7 0.18669579554221372 1106 8 0.2492617569878206 1106 9 0.08890935239789854 1106 10-11 0.06652797218003315 1106 12-13 0.03457068930718776 1106 14-15 0.09932108389208594 1106 16-17 0.21566978174967488 1106 18-19 0.21537053274036566 1106 20-21 0.0645229462321808 1106 22-23 0.0217330687631474 1106 24-25 0.08289790750557557 1106 26-27 0.02510209733872415 1106 28-29 0.027020448458699775 1106 30-31 -0.009832003833437852 1106 32-33 0.08454412854615612 1106 34-35 -0.02271090760033445 1106 36-37 0.10209226269055449 1106 38-39 0.0972286782456635 1106 40-41 0.0940345608043529 1106 42-43 0.0510119099419839 1106 44-45 -0.06834985774657554 1106 46-47 0.1198700514840425 1106 48-49 0.059941116489945045 1106 50-51 -0.03134819196963079 1106 52-53 0.009365800267080004 1106 54-55 0.2274769088564561 1106 56-57 0.1448355320651622 1106 58-59 -0.013074656306368837 1106 60-61 -0.037403019686863104 1106 62-63 -0.290569733082954 1106 64-65 -0.16809575939343446 1106 66-67 -0.19412442554037668 1106 68-69 -0.3187345967699784 1106 70-71 -0.36300507764766365 1106 72-73 -0.2801011988171034 1106 74-75 -0.3060315449574311 1106 76-77 -0.24626269197280948 1107 1 0.05567013954490818 1107 2 0.013491799739064447 1107 3 -0.06865998101226012 1107 4 -0.025522731224626227 1107 5 0.10045470814259971 1107 6 -0.18229143543319282 1107 7 -0.27352136241904645 1107 8 -0.1299252083382143 1107 9 -0.12178134095307058 1107 10-11 -0.12338198331655548 1107 12-13 -0.018075122185813086 1107 14-15 -0.12424494357875915 1107 16-17 -0.06109459342621193 1107 18-19 -0.0866851398708377 1107 20-21 -0.17916176168154863 1107 22-23 -0.20499741314265663 1107 24-25 -0.2893475893301627 1107 26-27 -0.3285273780833009 1107 28-29 -0.21717633660302482 1107 30-31 0.008605395116724424 1107 32-33 0.11348468486846741 1107 34-35 0.1081928289390035 1107 36-37 -0.03409595976199142 1107 38-39 -0.043967585934957754 1107 40-41 -0.052275118662819864 1107 42-43 0.08471266687864443 1107 44-45 0.06593044984874297 1107 46-47 0.04807790746041718 1107 48-49 0.18475619430751777 1107 50-51 0.10183541674097896 1107 52-53 0.06644829637690464 1107 54-55 0.0423418663611308 1107 56-57 0.041169236547151655 1107 58-59 -0.02608690170773187 1107 60-61 -0.09675458844990104 1107 62-63 -0.041833902754966346 1107 64-65 0.011637958924456626 1107 66-67 0.15103216277604403 1107 68-69 -0.026654822451735782 1107 70-71 0.006420962400706998 1107 72-73 0.0226726808737574 1107 74-75 0.14894401213336295 1107 76-77 0.33284436474531987 1108 1 0.09222724530020088 1108 2 0.1194656156059466 1108 3 0.10718940701224255 1108 4 -0.07241424726895218 1108 5 -0.08311223131032364 1108 6 -0.08868925776658898 1108 7 -0.06864080406016626 1108 8 0.004637357128096653 1108 9 0.07582318991354953 1108 10-11 0.0013104146994820098 1108 12-13 0.04639245435296857 1108 14-15 0.13691541839315136 1108 16-17 0.2055159137075222 1108 18-19 0.23829949497660152 1108 20-21 0.10001394272399722 1108 22-23 0.043705815811271975 1108 24-25 0.26392405221815807 1108 26-27 0.002604364049979324 1108 28-29 0.08708088681226656 1108 30-31 0.1217547970117181 1108 32-33 0.12390698143862267 1108 34-35 0.1211467884850066 1108 36-37 0.24706525715809136 1108 38-39 0.11603282659248038 1108 40-41 0.25457504897877214 1108 42-43 0.19907753006301476 1108 44-45 0.19739711643569535 1108 46-47 0.2556951967844441 1108 48-49 0.32143266398136916 1108 50-51 0.4036192691015934 1108 52-53 0.26422008021731 1108 54-55 0.30977085185451614 1108 56-57 0.23222041027739948 1108 58-59 0.15046064417249383 1108 60-61 0.11449966835500902 1108 62-63 0.10748488888638974 1108 64-65 0.1356803272863516 1108 66-67 0.014356888833908954 1108 68-69 0.05077822255082509 1108 70-71 0.016479009196860517 1108 72-73 -0.03956978840789205 1108 74-75 -0.13994342527008286 1108 76-77 -0.15627776490069323 1109 1 0.07228814699580965 1109 2 0.03638208389178743 1109 3 -0.10619198803091479 1109 4 0.15024540669647024 1109 5 0.08367918403339303 1109 6 0.04082877419828179 1109 7 -0.04943346323948106 1109 8 0.07328959676239322 1109 9 0.10142861654185253 1109 10-11 -0.05133322275286645 1109 12-13 0.0262534539002246 1109 14-15 0.1359496810737042 1109 16-17 0.17006720792941366 1109 18-19 -0.010635273027368441 1109 20-21 -0.08676084149121266 1109 22-23 0.09721569590649182 1109 24-25 -0.06652119710879845 1109 26-27 -0.0978294266545987 1109 28-29 0.03999799770593171 1109 30-31 0.11722946268525902 1109 32-33 0.19040806705638147 1109 34-35 0.2401685884684568 1109 36-37 0.29080229546628544 1109 38-39 0.25681612049306324 1109 40-41 0.2843762908670655 1109 42-43 0.34539403632114585 1109 44-45 0.26993436456506714 1109 46-47 0.12157698128688565 1109 48-49 0.24927483438928277 1109 50-51 0.3433556173366128 1109 52-53 0.4264285030045727 1109 54-55 0.4643265518345565 1109 56-57 0.43552226434700714 1109 58-59 0.36387748609584136 1109 60-61 0.38001338340712465 1109 62-63 0.4384090514272252 1109 64-65 0.4732823473629786 1109 66-67 0.4576980174382328 1109 68-69 0.5727566574600047 1109 70-71 0.5926674381181094 1109 72-73 0.46376358752254987 1109 74-75 0.5336743289616344 1109 76-77 0.6188250158385955 1110 1 -0.20996076351396908 1110 2 -0.3507112575493778 1110 3 -0.41833853713398383 1110 4 -0.15215653918373562 1110 5 -0.12533064671556815 1110 6 -0.27627368855635837 1110 7 -0.34182223388911837 1110 8 -0.17128828911983618 1110 9 -0.3721702483613569 1110 10-11 -0.3062029906661152 1110 12-13 -0.32689339924662875 1110 14-15 -0.2986631698435005 1110 16-17 -0.27278522948824246 1110 18-19 -0.24015792428915006 1110 20-21 -0.36224984524108805 1110 22-23 -0.39415959546879975 1110 24-25 -0.2752219166790866 1110 26-27 -0.2908212174724767 1110 28-29 -0.25745563561726925 1110 30-31 -0.30414481521075487 1110 32-33 -0.10275854893632186 1110 34-35 0.13188086170247004 1110 36-37 0.005561087398980646 1110 38-39 -0.030132800151513095 1110 40-41 0.02013944076065144 1110 42-43 0.025395556540054542 1110 44-45 0.02981325379179367 1110 46-47 -0.09547734120221918 1110 48-49 -0.11478465684412242 1110 50-51 -0.05267154390794104 1110 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-0.24528973099553042 2314 24-25 -0.3187420304453781 2314 26-27 -0.3556194665803005 2314 28-29 -0.39723391644744055 2314 30-31 -0.4493261363702601 2314 32-33 -0.5900428723742834 2314 34-35 -0.5988055901929172 2314 36-37 -0.6620596413442641 2314 38-39 -0.5641313645818187 2314 40-41 -0.48098770527345636 2314 42-43 -0.36402688268355377 2314 44-45 -0.4281828111160735 2314 46-47 -0.4060996677142974 2314 48-49 -0.3491479393744825 2314 50-51 -0.23882390746213034 2314 52-53 -0.18365640235964875 2314 54-55 -0.25682862396541495 2314 56-57 -0.19835993393553153 2314 58-59 -0.3140439215700326 2314 60-61 -0.24830489444039827 2314 62-63 -0.2609186535464829 2314 64-65 -0.18302598680705984 2314 66-67 -0.11443291755076146 2314 68-69 0.13701491988104308 2314 70-71 0.14839949068440106 2314 72-73 0.16721377923806457 2314 74-75 0.23496520779675834 2314 76-77 0.15374564180226358 2315 1 -1.6778579711325747 2315 2 -1.5385351356346604 2315 3 -1.5138166220426186 2315 4 -1.42266938729545 2315 5 -1.3862149377412152 2315 6 -1.5177907962329016 2315 7 -1.4980530759089632 2315 8 -1.4470721030230607 2315 9 -1.545509858882447 2315 10-11 -1.5561321539126212 2315 12-13 -1.4459314358221604 2315 14-15 -1.7028110431653616 2315 16-17 -1.5777085164508833 2315 18-19 -1.5430125159802373 2315 20-21 -1.5372943112542643 2315 22-23 -1.5105470177566147 2315 24-25 -1.7905819954496849 2315 26-27 -1.690172189952996 2315 28-29 -1.6516734471687258 2315 30-31 -1.7525568458217151 2315 32-33 -1.7952207134367058 2315 34-35 -1.656448248880558 2315 36-37 -1.856403304223896 2315 38-39 -1.7004636971747402 2315 40-41 -1.711527286743575 2315 42-43 -1.9124307082493175 2315 44-45 -1.8279009950188367 2315 46-47 -1.7597548702739907 2315 48-49 -1.739747397741347 2315 50-51 -1.658430270899256 2315 52-53 -1.778891688408855 2315 54-55 -1.841864712175692 2315 56-57 -1.815112498145698 2315 58-59 -1.6875363288914187 2315 60-61 -1.6792822968552592 2315 62-63 -1.7696850745184207 2315 64-65 -1.8079808603562846 2315 66-67 -1.9464864718122534 2315 68-69 -1.8818213607769074 2315 70-71 -1.8263226981822562 2315 72-73 -3.1773373564771177 2315 74-75 -3.7943352293022734 2315 76-77 -3.4397783522436605 2316 1 -3.653088275984331 2316 2 -3.538254546959365 2316 3 -2.9454059223206244 2316 4 -3.010315517934906 2316 5 -3.663635102709762 2316 6 -3.1488374678437623 2316 7 -3.357617615288106 2316 8 -3.1508012489008053 2316 9 -2.9315738696878526 2316 10-11 -3.088601911112903 2316 12-13 -3.1997781363715205 2316 14-15 -3.136948652087078 2316 16-17 -4.4523746162459865 2316 18-19 -4.183110594534227 2316 20-21 -3.5497273471606263 2316 22-23 -3.735063277972337 2316 24-25 -3.6732349926077816 2316 26-27 -3.489725173056165 2316 28-29 -3.612111208846194 2316 30-31 -3.899088576659281 2316 32-33 -4.086889932406461 2316 34-35 -3.9281981896809306 2316 36-37 -3.8477790906705494 2316 38-39 -3.7909681416878875 2316 40-41 -3.862956188715696 2316 42-43 -4.149292041542708 2316 44-45 -3.868620613821083 2316 46-47 -3.6077831484111798 2316 48-49 -3.7018229253031905 2316 50-51 -4.151532232247241 2316 52-53 -5.019900819651628 2316 54-55 -4.2943300138674765 2316 56-57 -4.218336718264389 2316 58-59 -4.159816755664117 2316 60-61 -3.9265495053007093 2316 62-63 -3.949627637340928 2316 64-65 -3.823636873428324 2316 66-67 -4.086036631181138 2316 68-69 -4.069218199660558 2316 70-71 -3.845604182102427 2316 72-73 -3.89507190569471 2316 74-75 -3.8633477095066517 2316 76-77 -3.827149790266997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97614.0 3 7603.0 4 9438.0 5 10931.0 6 13016.0 7 13700.0 8 15518.0 9 15764.0 10 17776.0 11 19380.0 12 17151.0 13 15843.0 14 16192.0 15 17034.0 16 17628.0 17 18590.0 18 19724.0 19 21019.0 20 22408.0 21 24133.0 22 26906.0 23 30450.0 24 35650.0 25 41855.0 26 49111.0 27 58136.0 28 69303.0 29 81540.0 30 96851.0 31 116859.0 32 142415.0 33 178845.0 34 237074.0 35 316334.0 36 452640.0 37 618295.0 38 558928.0 39 142763.0 40 1008.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.001077747916128 26.790930546650117 27.814463649881993 22.393528055551766 2 22.69081778798627 26.81805320330059 27.78535865555702 22.705770353156122 3 22.914104645867447 27.123536083939783 27.385506831949353 22.57685243824342 4 22.494349726743152 27.00182571427558 27.79167702402297 22.712147534958294 5 22.814711049713054 27.00745680800377 27.593654850936144 22.58417729134703 6 22.98772734962047 26.91549388004562 27.634799975989477 22.461978794344432 7 22.937592483800753 26.560624487186445 27.672271927409476 22.82951110160332 8 22.76618984872493 27.012364449608313 27.52961227423958 22.691833427427177 9 22.74223700191198 26.622815119779062 27.71215477889723 22.922793099411727 10-11 23.113643697160256 26.858311543103042 27.413421781316366 22.614622978420336 12-13 23.10497359076865 26.654455051916642 27.39026259701371 22.85030876030099 14-15 22.865809488080217 26.88844173118243 27.5496879369735 22.696060843763853 16-17 23.135234624752364 26.682005558779583 27.55916264163988 22.623597174828173 18-19 22.992349736020664 26.680297333356435 27.407913527035966 22.91943940358693 20-21 22.982068669297178 26.885316627751166 27.359070186667527 22.773544516284137 22-23 22.972752643998398 26.844659167577962 27.124821010364514 23.057767178059127 24-25 22.996032449866703 26.97029521956597 27.106389829537235 22.92728250103009 26-27 23.05893990715063 26.92934647278135 27.064820594686235 22.946893025381783 28-29 23.20453158917753 26.862423999676956 27.069470652497767 22.86357375864775 30-31 22.98996890948554 26.731553393422562 27.18958938420591 23.088888312885988 32-33 22.929808571062566 27.10997951864938 27.1091609740299 22.851050936258158 34-35 23.057487613538864 26.82876717654989 27.163788600212907 22.949956609698337 36-37 23.105309075927064 26.822364817438483 27.362296972917445 22.710029133717008 38-39 22.983974720162667 26.808461465629186 27.35042758364014 22.857136230568006 40-41 22.871877230987806 26.808006501506892 27.24031250780185 23.079803759703456 42-43 22.79229751676446 27.10131474399273 27.033413772177983 23.072973967064822 44-45 22.779070130721298 26.832676000075857 27.285780960069 23.102472909133848 46-47 22.804194391114567 26.809463235768654 27.281238773380874 23.1051035997359 48-49 22.886949113552447 26.78604125830827 27.204043802125817 23.122965826013466 50-51 22.816647710254134 26.92902705501954 27.08706008216189 23.167265152564436 52-53 22.860419524994672 26.846731209953273 27.167473480493047 23.12537578455901 54-55 22.808273346152188 26.814774830435812 27.21267145399758 23.164280369414424 56-57 22.760283454158913 27.063437726232443 27.104614930379416 23.071663889229228 58-59 22.886496415792614 27.02939320191649 27.091224224226686 22.992886158064213 60-61 22.890101453481194 26.88552043156785 26.974218380872895 23.250159734078064 62-63 22.827780515030792 26.75875480169591 27.250982784505386 23.162481898767908 64-65 22.84646944074265 26.760865670635773 27.137437008998333 23.25522787962325 66-67 22.792808748615393 26.897849769007014 27.26452674205333 23.04481474032426 68-69 22.845483155046608 26.663462432055013 27.354556338373182 23.136498074525203 70-71 22.760325883937593 26.646879315858286 27.28873304314251 23.30406175706161 72-73 22.839900668422494 26.52144654715731 27.232793127886605 23.405859656533586 74-75 22.674430193654015 26.517515310930186 27.21511867148447 23.592935823931334 76-77 22.612778605703426 26.413715090873467 27.52997428386328 23.44353201955983 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4725.0 1 2906.5 2 1025.0 3 1004.0 4 1046.0 5 1127.5 6 1216.5 7 1317.0 8 1453.5 9 1497.0 10 1542.0 11 1656.5 12 1834.0 13 1942.0 14 2049.0 15 2335.5 16 2764.5 17 3014.0 18 3308.0 19 3995.0 20 5104.0 21 6887.0 22 7954.0 23 9545.5 24 13161.5 25 18397.0 26 21608.0 27 25497.0 28 34986.0 29 45392.0 30 50198.0 31 57684.5 32 72734.0 33 86996.0 34 99517.5 35 105340.0 36 113607.5 37 128629.0 38 138990.0 39 142597.0 40 147427.0 41 157103.0 42 168852.0 43 175755.0 44 177102.0 45 178082.5 46 180699.5 47 185156.5 48 186630.0 49 186196.0 50 183564.0 51 176169.5 52 170973.0 53 167429.0 54 156154.5 55 137862.0 56 118122.5 57 108945.0 58 102459.5 59 86081.0 60 69780.5 61 63373.0 62 55402.5 63 39994.5 64 28506.5 65 24456.0 66 21060.5 67 15685.5 68 11477.0 69 7805.5 70 6363.0 71 5422.5 72 3761.5 73 2693.5 74 2346.0 75 1973.5 76 1309.0 77 824.0 78 631.0 79 505.0 80 333.0 81 235.0 82 150.5 83 118.0 84 112.0 85 95.5 86 84.5 87 84.0 88 79.0 89 63.5 90 59.5 91 66.0 92 61.0 93 47.0 94 39.5 95 49.0 96 57.0 97 57.0 98 65.0 99 121.5 100 170.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010230737226924573 2 0.013695546901109693 3 0.013968366560494348 4 0.015223336993663763 5 0.012331448604186416 6 0.009957917567539918 7 0.016205487767448522 8 0.017569586064371798 9 0.017351330336864073 10-11 0.02039326953900298 12-13 0.016587435290587038 14-15 0.008184589781539657 16-17 0.0063294160977240025 18-19 0.01771963687703336 20-21 0.02350341365598805 22-23 0.020666089198387635 24-25 0.01896096632723354 26-27 0.024212744770388154 28-29 0.008430127474985848 30-31 0.008730229100308969 32-33 0.01046263393740153 34-35 0.011881296166201736 36-37 0.011663040438694013 38-39 0.014404878015509796 40-41 0.011690322404632478 42-43 0.014432159981448263 44-45 0.01338180429281734 46-47 0.023585259553803445 48-49 0.023298798911449557 50-51 0.035943990123928335 52-53 0.038849519496374905 54-55 0.02812770688255796 56-57 0.021170805568249247 58-59 0.016314615631202383 60-61 0.02020229577743372 62-63 0.02631345614765 64-65 0.024922075884788255 66-67 0.028045860984742564 68-69 0.04156407510725223 70-71 0.053840959779561715 72-73 0.08581542385944331 74-75 0.08653839595681265 76-77 0.09078074166024404 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3665425.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.027995510271886 #Duplication Level Percentage of deduplicated Percentage of total 1 75.03012558802247 41.28757413992836 2 13.426555707331245 14.776728943628784 3 4.924516967636442 8.129588927560677 4 2.260326518060218 4.975249499502646 5 1.2461025385352622 3.428526244792841 6 0.7192819318695418 2.3748385750522103 7 0.49923775225562494 1.923043695277653 8 0.33743164800492137 1.4854549769150764 9 0.24244127765937917 1.2006951784690407 >10 1.1810364508375808 11.672654567353948 >50 0.08487903863808939 3.2537310014966203 >100 0.045969650672091224 4.538385417709232 >500 0.0018455339953813118 0.6829231711117836 >1k 2.4939648174240837E-4 0.27060566120129403 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4762 0.12991672179897285 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.7281965938465527E-5 0.0 2 0.0 0.0 0.0 5.4563931876931055E-5 0.0 3 0.0 0.0 0.0 1.9097376156925868E-4 0.0 4 0.0 0.0 0.0 7.366130803385692E-4 0.0 5 0.0 0.0 0.0 0.001609635990369466 0.0 6 0.0 0.0 0.0 0.004010448992954432 0.0 7 2.7281965938465527E-5 0.0 0.0 0.007884488156216536 0.0 8 2.7281965938465527E-5 0.0 0.0 0.012113192876678693 0.0 9 2.7281965938465527E-5 0.0 0.0 0.016832972984033228 0.0 10 2.7281965938465527E-5 0.0 0.0 0.02207111044421861 0.0 11 2.7281965938465527E-5 0.0 0.0 0.02834596261006568 0.0 12 2.7281965938465527E-5 0.0 0.0 0.036721526153174595 0.0 13 2.7281965938465527E-5 0.0 0.0 0.04676128961852991 0.0 14 2.7281965938465527E-5 0.0 0.0 0.06154811515717823 0.0 15 2.7281965938465527E-5 0.0 0.0 0.07715339967398051 0.0 16 2.7281965938465527E-5 0.0 0.0 0.0933043235095521 0.0 17 2.7281965938465527E-5 0.0 0.0 0.1082548408438312 0.0 18 2.7281965938465527E-5 0.0 0.0 0.12402381715626427 0.0 19 2.7281965938465527E-5 0.0 0.0 0.1408022262084206 0.0 20 2.7281965938465527E-5 0.0 0.0 0.15856278603436164 0.0 21 2.7281965938465527E-5 0.0 0.0 0.1789969785222723 0.0 22 2.7281965938465527E-5 0.0 0.0 0.2007679873411678 0.0 23 2.7281965938465527E-5 0.0 0.0 0.22423047804824817 0.0 24 2.7281965938465527E-5 0.0 0.0 0.24897522115443638 0.0 25 2.7281965938465527E-5 0.0 0.0 0.2733380167374861 0.0 26 2.7281965938465527E-5 0.0 0.0 0.29958326797028995 0.0 27 2.7281965938465527E-5 0.0 0.0 0.32577395527121683 0.0 28 2.7281965938465527E-5 0.0 0.0 0.3522374622315284 0.0 29 2.7281965938465527E-5 0.0 0.0 0.379819529795317 0.0 30 2.7281965938465527E-5 0.0 0.0 0.4097205644638753 0.0 31 2.7281965938465527E-5 0.0 0.0 0.4431955366703725 0.0 32 2.7281965938465527E-5 0.0 0.0 0.47849840059474685 0.0 33 2.7281965938465527E-5 0.0 0.0 0.5180572512055218 0.0 34 2.7281965938465527E-5 0.0 0.0 0.5593621476363587 0.0 35 2.7281965938465527E-5 0.0 0.0 0.6007761719309493 0.0 36 2.7281965938465527E-5 0.0 0.0 0.6439635240115402 0.0 37 2.7281965938465527E-5 0.0 0.0 0.6903428661069316 0.0 38 2.7281965938465527E-5 0.0 0.0 0.7376770770101694 0.0 39 2.7281965938465527E-5 0.0 0.0 0.7859115927893764 0.0 40 2.7281965938465527E-5 0.0 0.0 0.835701180627076 2.7281965938465527E-5 41 2.7281965938465527E-5 0.0 0.0 0.8898013190830532 2.7281965938465527E-5 42 2.7281965938465527E-5 0.0 0.0 0.9495761064542311 2.7281965938465527E-5 43 2.7281965938465527E-5 0.0 0.0 1.0128157034995942 2.7281965938465527E-5 44 2.7281965938465527E-5 0.0 0.0 1.0817572314260966 2.7281965938465527E-5 45 2.7281965938465527E-5 0.0 0.0 1.1532632641508147 2.7281965938465527E-5 46 2.7281965938465527E-5 0.0 0.0 1.2332539882823956 2.7281965938465527E-5 47 2.7281965938465527E-5 0.0 0.0 1.3110621551388992 2.7281965938465527E-5 48 2.7281965938465527E-5 0.0 0.0 1.3942994332171577 2.7281965938465527E-5 49 2.7281965938465527E-5 0.0 0.0 1.4765272785556927 2.7281965938465527E-5 50 2.7281965938465527E-5 0.0 0.0 1.5636658777631516 2.7281965938465527E-5 51 2.7281965938465527E-5 0.0 0.0 1.6569702012727037 2.7281965938465527E-5 52 2.7281965938465527E-5 0.0 0.0 1.7579953211428416 2.7281965938465527E-5 53 2.7281965938465527E-5 0.0 0.0 1.8653498571107034 2.7281965938465527E-5 54 2.7281965938465527E-5 0.0 0.0 1.9787064255850277 2.7281965938465527E-5 55 2.7281965938465527E-5 0.0 0.0 2.0959643151885525 2.7281965938465527E-5 56 2.7281965938465527E-5 0.0 0.0 2.2213249486758015 2.7281965938465527E-5 57 2.7281965938465527E-5 0.0 0.0 2.3507778770538206 2.7281965938465527E-5 58 2.7281965938465527E-5 0.0 0.0 2.487951601792425 2.7281965938465527E-5 59 2.7281965938465527E-5 0.0 0.0 2.6276625493633072 2.7281965938465527E-5 60 2.7281965938465527E-5 0.0 0.0 2.772502506530621 2.7281965938465527E-5 61 2.7281965938465527E-5 0.0 0.0 2.926727459980766 2.7281965938465527E-5 62 2.7281965938465527E-5 0.0 0.0 3.0819891281365734 2.7281965938465527E-5 63 2.7281965938465527E-5 0.0 0.0 3.2480271728380745 2.7281965938465527E-5 64 2.7281965938465527E-5 0.0 0.0 3.4204219156032383 2.7281965938465527E-5 65 2.7281965938465527E-5 0.0 0.0 3.6051481069725884 2.7281965938465527E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCGCG 380 0.0 29.894497 56 CTCGCGC 425 0.0 29.23067 36 TCGCGCT 470 0.0 26.434515 37 CGGTTAC 355 0.0 25.993406 4 CGCGGAA 410 0.0 25.974339 59 CGATCGT 510 0.0 25.053833 1 TCGCGGA 485 0.0 22.68899 58 CCTCGCG 565 0.0 22.616508 35 CAATTCG 475 0.0 22.423014 54 CGCGCTT 570 0.0 21.795988 38 AATTCGC 490 0.0 21.734222 55 CGCTCCG 630 0.0 20.847046 25 CGTTCGG 145 9.908699E-6 19.582842 11 GCGCCCT 710 0.0 18.496822 31 GACGCTC 655 0.0 18.427332 23 ACTCGAG 695 0.0 18.385582 14 ATCGTTC 665 0.0 18.145525 3 ACGATCG 380 0.0 17.74913 59 AACTCGA 740 0.0 17.267775 13 CGCGAGG 415 0.0 17.10666 25 >>END_MODULE