FastQCFastQC Report
Thu 26 May 2016
SRR936041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3665425
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58940.16079990724131582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40830.11139226692675475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13550.038.21825100-101
GGTATCA72100.034.1689341
CGGTATC9500.033.521313100-101
CGTACAT19650.032.866844100-101
GTATCAA134100.029.864321
CCTGTCG9550.027.7333996-97
CCGGTAT4850.026.386848100-101
TCGTATC6450.025.8398100-101
GCGTACA7400.025.740032100-101
TACGCAG10550.025.389307100-101
TCGAGTA5050.025.341825100-101
ACGTATC5100.024.509808100-101
CCGTACA11000.024.350655100-101
TCGGTAT5000.023.80953100-101
ATCAACG170050.023.371643
CCGTATC6400.023.251493100-101
TCAACGC170950.023.2131564
TATCAAC173500.022.9078372
CGAGTAC12600.022.675743100-101
CCGTATA4500.022.486778100-101
Produced by FastQC (version 0.11.5)