FastQCFastQC Report
Thu 26 May 2016
SRR936040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677361
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49160.13368282308971027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG3600.028.59402356
CGCGGAA3800.025.22429559
AATTCGC4250.025.05704355
CTCGCGC4600.023.91993336
CGATCGT4650.022.8994181
CGCGCTT5100.022.27262538
CCTCGCG5400.021.0326835
TCGCGCT5300.020.76182737
TCGCGGA4650.020.6125658
CGGTTAC4250.020.0434534
CAATTCG5700.019.92976454
TCGCGTA1309.486104E-519.11714761
GTCGGGT4850.019.03915666
ATCGTTC5900.018.6491873
GACGTAT4550.017.16384939
CGTTCGG1452.1922021E-417.13624811
CGCTCCG6500.016.92910625
ACGCTCC6850.016.5838924
GATCGTT6900.016.4610312
ACTCGAG6950.016.34238414
Produced by FastQC (version 0.11.5)