##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936040_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3677361 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.478251659274136 39.0 36.0 41.0 30.0 41.0 2 36.34882759674669 39.0 36.0 41.0 29.0 41.0 3 36.21529270582899 39.0 36.0 41.0 29.0 41.0 4 36.106522585082075 39.0 36.0 41.0 29.0 41.0 5 36.03978831558827 39.0 35.0 41.0 28.0 41.0 6 35.95588657191937 39.0 35.0 40.0 28.0 41.0 7 35.82561842582221 39.0 35.0 40.0 27.0 41.0 8 35.703390284500216 39.0 35.0 40.0 27.0 41.0 9 35.88670380743147 39.0 35.0 40.0 28.0 41.0 10-11 36.11638753986894 39.0 35.0 41.0 29.0 41.0 12-13 36.051519690343156 39.0 35.0 41.0 28.5 41.0 14-15 35.91316517470001 39.0 35.0 41.0 27.5 41.0 16-17 35.780611558125514 39.0 35.0 41.0 27.0 41.0 18-19 35.647019289104335 39.0 35.0 41.0 27.0 41.0 20-21 35.47463806245838 39.0 35.0 41.0 26.5 41.0 22-23 35.24189860609279 39.0 35.0 41.0 25.5 41.0 24-25 35.05144681199371 39.0 34.5 41.0 24.5 41.0 26-27 34.115393892522384 38.0 33.5 40.0 23.5 40.5 28-29 34.13610956335263 38.0 33.5 39.5 23.5 40.5 30-31 34.54616462729659 38.0 34.0 40.0 24.0 41.0 32-33 34.42789108820156 38.0 34.0 40.0 23.0 41.0 34-35 34.17580922841135 38.0 33.5 40.0 23.0 41.0 36-37 33.867363307545816 38.0 33.0 40.0 22.0 41.0 38-39 33.5544083107424 37.0 33.0 40.0 21.5 41.0 40-41 33.19591168775652 37.0 32.5 40.0 20.0 41.0 42-43 32.826842945253404 36.0 32.0 39.0 18.5 41.0 44-45 32.46546572392539 36.0 32.0 39.0 18.0 41.0 46-47 32.045027806625455 35.0 31.5 38.5 14.5 40.0 48-49 31.622578392493963 35.0 31.0 37.5 11.5 40.0 50-51 31.15960739236643 35.0 31.0 37.0 7.5 39.0 52-53 30.66833471067975 35.0 30.0 36.5 6.0 39.0 54-55 30.20764618975401 34.5 30.0 36.0 2.0 38.5 56-57 29.757323254366376 34.0 29.5 36.0 2.0 37.0 58-59 29.330602706669268 34.0 29.0 35.0 2.0 37.0 60-61 28.967433983228734 34.0 29.0 35.0 2.0 36.5 62-63 28.596578225526404 34.0 29.0 35.0 2.0 36.0 64-65 28.219411284342222 34.0 28.5 35.0 2.0 36.0 66-67 27.868221939592004 34.0 27.5 35.0 2.0 35.0 68-69 27.49853359515152 34.0 27.0 35.0 2.0 35.0 70-71 27.04944714429723 33.0 26.0 35.0 2.0 35.0 72-73 26.56770276293244 33.0 25.0 35.0 2.0 35.0 74-75 25.99170872807973 33.0 24.5 35.0 2.0 35.0 76-77 24.22309626386966 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.2465481462205972 1101 2 1.131750034971624 1101 3 1.2406649449123535 1101 4 1.1630068009305248 1101 5 1.203293219455162 1101 6 1.2320453650492027 1101 7 1.2081037370640004 1101 8 1.111404312222028 1101 9 1.1880013835271157 1101 10-11 1.1507191975670494 1101 12-13 1.3321667982920715 1101 14-15 1.3806466716179244 1101 16-17 1.3331086260446838 1101 18-19 1.2995372458407033 1101 20-21 1.4564915840603447 1101 22-23 1.3767251452809859 1101 24-25 1.4658767819055285 1101 26-27 1.4393174552343595 1101 28-29 1.515085639146477 1101 30-31 1.5305963535970548 1101 32-33 1.5858043558026438 1101 34-35 1.4569813853155011 1101 36-37 1.4858932786165298 1101 38-39 1.5872263069485157 1101 40-41 1.4488551318523548 1101 42-43 1.4332233708587623 1101 44-45 1.413899394849068 1101 46-47 1.492745174480497 1101 48-49 1.4112431101893144 1101 50-51 1.4487359891152352 1101 52-53 1.3928345666334145 1101 54-55 1.473749836718742 1101 56-57 1.47502489900209 1101 58-59 1.6588175349654186 1101 60-61 1.5311754526815236 1101 62-63 1.6098584181454605 1101 64-65 1.5613007814400106 1101 66-67 1.6238920090594497 1101 68-69 1.599496034541609 1101 70-71 1.6576484951518715 1101 72-73 1.5336568389369347 1101 74-75 1.6430529477760842 1101 76-77 1.6352589197529568 1102 1 0.8181814856421852 1102 2 0.8168517853620045 1102 3 0.908193575867827 1102 4 0.8267135211738363 1102 5 0.8226302492777506 1102 6 0.8956431599443846 1102 7 0.8239094088009296 1102 8 0.8672032902794768 1102 9 0.9077008345977831 1102 10-11 0.9698281218947997 1102 12-13 0.9121985286456251 1102 14-15 0.8162210695864118 1102 16-17 0.8762729949902237 1102 18-19 0.9690443471611658 1102 20-21 0.932859162638799 1102 22-23 0.8610855570647118 1102 24-25 1.0956534687858905 1102 26-27 0.8360917683754323 1102 28-29 0.8868660915616644 1102 30-31 1.030931717822675 1102 32-33 1.0658628540715753 1102 34-35 0.9972183749011876 1102 36-37 0.9660769857653264 1102 38-39 1.0866104238130916 1102 40-41 1.07799442262845 1102 42-43 1.1342570477533584 1102 44-45 0.968664908590668 1102 46-47 1.075085382440335 1102 48-49 0.8362233063535705 1102 50-51 0.8580141414284128 1102 52-53 1.0203308363016284 1102 54-55 1.1286191058934314 1102 56-57 1.112144948510828 1102 58-59 1.172889652559217 1102 60-61 1.0704491629492807 1102 62-63 0.9893857076440362 1102 64-65 0.9320621313592454 1102 66-67 1.0170305879518438 1102 68-69 1.0409165180170987 1102 70-71 0.9206355258077679 1102 72-73 0.8903925834914297 1102 74-75 0.7940338541234127 1102 76-77 0.7936933895492579 1103 1 0.6461654858255201 1103 2 0.6796568938746645 1103 3 0.6295159785375759 1103 4 0.698699242912781 1103 5 0.5716289417890792 1103 6 0.6349886440222647 1103 7 0.5712988210690639 1103 8 0.6257928709771505 1103 9 0.6799045040897695 1103 10-11 0.6984733184669452 1103 12-13 0.766174482098009 1103 14-15 0.6559253154994806 1103 16-17 0.591487024234091 1103 18-19 0.6888616664029854 1103 20-21 0.6172268128580072 1103 22-23 0.6494567967982618 1103 24-25 0.7419496718094294 1103 26-27 0.8206394383771922 1103 28-29 0.7675553798735066 1103 30-31 0.8744115320390904 1103 32-33 0.912873077312419 1103 34-35 0.8476420742689754 1103 36-37 0.8587410765834065 1103 38-39 0.7086160328002009 1103 40-41 0.8410835498968652 1103 42-43 0.8646114760232066 1103 44-45 0.857271464960931 1103 46-47 0.882853963951554 1103 48-49 1.0037207173017713 1103 50-51 0.8833244051691835 1103 52-53 0.8466613302992201 1103 54-55 0.9341118030898166 1103 56-57 1.071746260500415 1103 58-59 1.029494679610405 1103 60-61 0.8963252384784539 1103 62-63 0.8190327351619722 1103 64-65 0.8164201944822373 1103 66-67 0.8794766131359815 1103 68-69 0.8523236209919958 1103 70-71 0.6983597717389607 1103 72-73 0.7580453993732696 1103 74-75 0.8554683870747901 1103 76-77 0.8886513930393534 1104 1 0.7281728880020992 1104 2 0.6724675674326619 1104 3 0.7766730519232965 1104 4 0.7375807029144852 1104 5 0.6230385105296534 1104 6 0.6415319076910038 1104 7 0.638987669224278 1104 8 0.7464413377049155 1104 9 0.5250472639297072 1104 10-11 0.7161887102864952 1104 12-13 0.7706268061711086 1104 14-15 0.9190957308327015 1104 16-17 0.8399757882508894 1104 18-19 0.6780138216984497 1104 20-21 0.8177992545245445 1104 22-23 0.8086674483200085 1104 24-25 0.873488553269425 1104 26-27 0.5825214843428057 1104 28-29 0.7697953145660748 1104 30-31 0.917069203909314 1104 32-33 0.9115296173207312 1104 34-35 0.996425573124597 1104 36-37 0.9543633752647338 1104 38-39 0.9165756263315572 1104 40-41 0.9058713758705892 1104 42-43 0.9081969240852672 1104 44-45 0.8260538620040734 1104 46-47 0.7199322076624597 1104 48-49 0.6841738126436177 1104 50-51 0.6586861251986313 1104 52-53 0.6762422408971531 1104 54-55 0.7970826401167272 1104 56-57 0.836510547752944 1104 58-59 0.6612902332962172 1104 60-61 0.566905843573835 1104 62-63 0.5660127673148629 1104 64-65 0.5047124352182486 1104 66-67 0.5226371121568754 1104 68-69 0.4182933912640934 1104 70-71 0.4477975969614505 1104 72-73 0.5742956310043006 1104 74-75 0.6518158786985104 1104 76-77 0.8973456951663543 1105 1 0.161286147678517 1105 2 0.5337123674051796 1105 3 0.39832339439264786 1105 4 0.4167484312981671 1105 5 0.15368371953759663 1105 6 0.22035568747991618 1105 7 0.36908918368605725 1105 8 0.2976223010472907 1105 9 0.3931758075593592 1105 10-11 0.5750290194584835 1105 12-13 0.6223087567910071 1105 14-15 0.6415862851300957 1105 16-17 0.4767683626259611 1105 18-19 0.43394321298386984 1105 20-21 0.3782448321033769 1105 22-23 0.40913307684216704 1105 24-25 0.4937014024788837 1105 26-27 0.4587744297128111 1105 28-29 0.5688516509409354 1105 30-31 0.6693341206754795 1105 32-33 0.6947083932874776 1105 34-35 0.5414900921166819 1105 36-37 0.5839818442735805 1105 38-39 0.5311659085430946 1105 40-41 0.6376284531091869 1105 42-43 0.719547227712475 1105 44-45 0.4985091529813417 1105 46-47 0.5388168846762085 1105 48-49 0.3979723641275861 1105 50-51 0.4239421636246732 1105 52-53 0.306888547410324 1105 54-55 0.19012407523365127 1105 56-57 0.25348785611659963 1105 58-59 0.3108379657072682 1105 60-61 0.43669543750644735 1105 62-63 0.34164881931179636 1105 64-65 0.4126944953023646 1105 66-67 0.38018475104138716 1105 68-69 0.2020085132637277 1105 70-71 0.15484338965130107 1105 72-73 -0.1972841848247775 1105 74-75 -0.42746002552650353 1105 76-77 -0.6534988589649906 1106 1 0.20207718278924602 1106 2 0.14726118229419427 1106 3 0.13763818877977485 1106 4 0.13298228931776634 1106 5 0.10666693643312186 1106 6 0.1737181079586918 1106 7 -0.17003758207601294 1106 8 -0.08793851874624892 1106 9 0.0498044975154599 1106 10-11 -0.11142467802299194 1106 12-13 0.29418929662664084 1106 14-15 0.2380112993238228 1106 16-17 0.1458255380734741 1106 18-19 0.11712683721214745 1106 20-21 0.1970718086697616 1106 22-23 0.23082274696088234 1106 24-25 0.04405834692919797 1106 26-27 0.07139824613977197 1106 28-29 0.3086356950872684 1106 30-31 0.44170067506031074 1106 32-33 0.3298132295771907 1106 34-35 0.23175179189733086 1106 36-37 0.20815646012743372 1106 38-39 0.296032332613386 1106 40-41 0.27351764207372753 1106 42-43 0.3970197876959105 1106 44-45 0.28861034281182185 1106 46-47 0.34558460029277427 1106 48-49 0.27790500442234034 1106 50-51 0.1963093050119653 1106 52-53 0.21621897484672203 1106 54-55 0.20638322131883413 1106 56-57 0.21265925284901854 1106 58-59 0.07960079464583103 1106 60-61 -0.03332681693047235 1106 62-63 -0.03300044672382185 1106 64-65 0.06684356950557913 1106 66-67 -0.004029015527930113 1106 68-69 0.22324708373900393 1106 70-71 0.059003029898633486 1106 72-73 0.0022112983087616556 1106 74-75 0.15172155687243816 1106 76-77 0.11921308766193306 1107 1 -0.03830551999946863 1107 2 0.15007575841703868 1107 3 0.12515658795231133 1107 4 0.10670628183875408 1107 5 0.061464644990230966 1107 6 -0.10472601822979044 1107 7 0.09592494328500578 1107 8 -0.03234318119601909 1107 9 -0.11261544122026379 1107 10-11 0.07193550815674854 1107 12-13 0.06910774353756466 1107 14-15 -0.028339100485226254 1107 16-17 -0.0013181147092709011 1107 18-19 0.05100418932257611 1107 20-21 0.028520470005645393 1107 22-23 0.06534456738495464 1107 24-25 -0.026693930589992476 1107 26-27 -0.21092959727829452 1107 28-29 -0.12353103125238363 1107 30-31 0.1747237088352236 1107 32-33 0.20508175804119588 1107 34-35 0.037963750364930604 1107 36-37 -0.01093293001459017 1107 38-39 0.06527687253461778 1107 40-41 0.0014833897763182335 1107 42-43 0.08874911100418359 1107 44-45 0.05859084963444161 1107 46-47 -0.03197636561405659 1107 48-49 -0.013652261758981155 1107 50-51 -0.011019532955970135 1107 52-53 -0.09335456217042193 1107 54-55 -0.07532302843252481 1107 56-57 -9.849767726564096E-4 1107 58-59 -0.046726929426252894 1107 60-61 -0.06269090174568603 1107 62-63 -0.1256775760550859 1107 64-65 -0.0997783424170855 1107 66-67 -0.15348002400149596 1107 68-69 -0.2881703487310574 1107 70-71 -0.25884516200970964 1107 72-73 -0.20641045448592266 1107 74-75 -0.5952649570313682 1107 76-77 -0.4264765332156628 1108 1 0.1499608101722032 1108 2 0.2982485701376234 1108 3 0.296329260857334 1108 4 0.2216893736481822 1108 5 0.32773735612642696 1108 6 0.1953599450136707 1108 7 0.197776594962626 1108 8 0.30630022665775414 1108 9 0.2014456649349512 1108 10-11 0.21122782187081413 1108 12-13 0.33235239800060157 1108 14-15 0.38080981220205956 1108 16-17 0.317126775512655 1108 18-19 0.2612304017968583 1108 20-21 0.309576469568718 1108 22-23 0.33087484283143453 1108 24-25 0.32825319433491273 1108 26-27 0.23772685013563688 1108 28-29 0.26383962564106156 1108 30-31 0.39814678680860993 1108 32-33 0.36682976455870886 1108 34-35 0.45045732161145224 1108 36-37 0.4579514764464818 1108 38-39 0.5075598935708641 1108 40-41 0.34101203129537083 1108 42-43 0.33715415433284335 1108 44-45 0.2977855657679953 1108 46-47 0.22251636591209234 1108 48-49 0.2971819514981284 1108 50-51 0.17392489530596578 1108 52-53 0.4298414328868354 1108 54-55 0.46969502896884663 1108 56-57 0.535591074812114 1108 58-59 0.47956112538085804 1108 60-61 0.5067689350436275 1108 62-63 0.41140210862878135 1108 64-65 0.47794514494842133 1108 66-67 0.5680929383681068 1108 68-69 0.5529920779925313 1108 70-71 0.56341687170654 1108 72-73 0.6031070021399039 1108 74-75 0.5544861705606152 1108 76-77 0.8146193587539727 1109 1 -0.11806667205706134 1109 2 -0.34550020996822894 1109 3 -0.21897076501576862 1109 4 -0.12699977916653893 1109 5 -0.13710330867616705 1109 6 -0.36477275109992036 1109 7 -0.2149196945165528 1109 8 -0.20807154487820156 1109 9 -0.1525965536156093 1109 10-11 -0.20723161636509957 1109 12-13 -0.10251107031165674 1109 14-15 -0.08723931605590707 1109 16-17 -0.058194226865857956 1109 18-19 -0.1290813974732501 1109 20-21 -0.13715217150324577 1109 22-23 -0.11358592991192751 1109 24-25 -0.177178029735245 1109 26-27 -0.3191746868175116 1109 28-29 -0.10428421404102295 1109 30-31 -0.0231232308032574 1109 32-33 -0.05773907378432597 1109 34-35 0.00454813734047832 1109 36-37 0.05167303589107064 1109 38-39 0.007345631930242291 1109 40-41 0.11474407899266481 1109 42-43 0.09643201671516266 1109 44-45 0.016323290001558632 1109 46-47 -0.07155216830848588 1109 48-49 0.05418641347374731 1109 50-51 0.0169279229130197 1109 52-53 0.11518073084058855 1109 54-55 0.14711260384197544 1109 56-57 0.08826305435420778 1109 58-59 0.17220531970602693 1109 60-61 0.12982178539722256 1109 62-63 0.13094106807609407 1109 64-65 0.2110598950006235 1109 66-67 0.18804172550963116 1109 68-69 0.043847765649697834 1109 70-71 0.07792822182864612 1109 72-73 0.0736685449485428 1109 74-75 0.24848685880787258 1109 76-77 0.3610465441234112 1110 1 -0.3103769586467564 1110 2 -0.19958425735650565 1110 3 -0.45344136910577504 1110 4 -0.35600145677753403 1110 5 -0.4826784393424788 1110 6 -0.3999051904140245 1110 7 -0.4429822155692804 1110 8 -0.5979392122473257 1110 9 -0.5560577980761678 1110 10-11 -0.5310579941583526 1110 12-13 -0.46635143668526524 1110 14-15 -0.41642387625326194 1110 16-17 -0.4327931151302451 1110 18-19 -0.6439947837682638 1110 20-21 -0.6217960153922348 1110 22-23 -0.606735145886546 1110 24-25 -0.6112884712427586 1110 26-27 -0.5755596780977044 1110 28-29 -0.3019069813158879 1110 30-31 -0.31810587034457427 1110 32-33 -0.3077115770422907 1110 34-35 -0.3424844927232442 1110 36-37 -0.28763035978793994 1110 38-39 -0.31504045343809395 1110 40-41 -0.22009527740048895 1110 42-43 -0.2090820126801276 1110 44-45 -0.3177422344249834 1110 46-47 -0.2605705545780381 1110 48-49 -0.35372665332220876 1110 50-51 -0.41703550222157304 1110 52-53 -0.35567733407429714 1110 54-55 -0.2819931208902382 1110 56-57 -0.19392744004616347 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-0.8350195313747122 2314 36-37 -1.4377123114362007 2314 38-39 -1.0242688867441743 2314 40-41 -0.8602627364391964 2314 42-43 -0.6392480210191636 2314 44-45 -0.8539130727969635 2314 46-47 -0.7432952441981158 2314 48-49 -0.7209282812217559 2314 50-51 -0.6257659278877767 2314 52-53 -0.4584981150205074 2314 54-55 -0.5798187671631574 2314 56-57 -0.6430935394059674 2314 58-59 -0.6005027439083328 2314 60-61 -0.6139046766986702 2314 62-63 -0.65660740328779 2314 64-65 -0.47058650603300123 2314 66-67 -0.360827886880557 2314 68-69 -0.4251618131697228 2314 70-71 -0.4186918484723954 2314 72-73 -0.3767190136632337 2314 74-75 -0.3400389511045816 2314 76-77 -0.41632704631852135 2315 1 -1.3459784553645164 2315 2 -1.170825111594958 2315 3 -1.159708874735891 2315 4 -0.9919994210787522 2315 5 -1.153489103383933 2315 6 -1.1648389721238104 2315 7 -1.5780296668076161 2315 8 -1.4595307526266126 2315 9 -1.0957530167976302 2315 10-11 -1.2455753796357811 2315 12-13 -1.0859326773289624 2315 14-15 -1.1383462612442727 2315 16-17 -1.069319787575857 2315 18-19 -1.0248748241918406 2315 20-21 -0.9905647733691296 2315 22-23 -1.194078076189271 2315 24-25 -1.4554979826895718 2315 26-27 -1.244617782632723 2315 28-29 -1.282579277372129 2315 30-31 -1.3778066030219023 2315 32-33 -1.2950699529554228 2315 34-35 -1.441521256542984 2315 36-37 -1.584759799448058 2315 38-39 -1.3924960150920285 2315 40-41 -1.4761372154089578 2315 42-43 -1.5806601112038834 2315 44-45 -1.6281648815013128 2315 46-47 -1.3500471934099352 2315 48-49 -1.2368340560348265 2315 50-51 -1.1910703965961034 2315 52-53 -1.3077262460208878 2315 54-55 -1.4493551122740946 2315 56-57 -1.6312080071087856 2315 58-59 -1.5184232201935295 2315 60-61 -1.6889996619431926 2315 62-63 -1.537750433744101 2315 64-65 -1.3330394360308588 2315 66-67 -1.4231382292401769 2315 68-69 -1.671716608448147 2315 70-71 -1.5359828458075846 2315 72-73 -1.428702084489096 2315 74-75 -1.405803965974222 2315 76-77 -1.546079793788401 2316 1 -2.367786364969035 2316 2 -1.9692728879240917 2316 3 -2.335843164175266 2316 4 -3.1073420257433924 2316 5 -2.4579118181456323 2316 6 -2.3220735078329184 2316 7 -1.9211551195093435 2316 8 -1.9180321288398545 2316 9 -2.1387422968208085 2316 10-11 -2.114068788298681 2316 12-13 -2.586306065710829 2316 14-15 -3.7902693381673487 2316 16-17 -2.999755829876314 2316 18-19 -2.4566718689549916 2316 20-21 -2.3456951514656197 2316 22-23 -2.566149277116409 2316 24-25 -2.6410462950175457 2316 26-27 -2.6069537235279867 2316 28-29 -3.190359228118176 2316 30-31 -3.2508107316616233 2316 32-33 -3.4215758199697603 2316 34-35 -3.178913332452005 2316 36-37 -3.177198594174989 2316 38-39 -3.3290688819615113 2316 40-41 -2.9962060260709933 2316 42-43 -3.223735947796669 2316 44-45 -3.094885148052086 2316 46-47 -3.004581091715025 2316 48-49 -2.831280673447548 2316 50-51 -2.9514651524993347 2316 52-53 -2.994353074935855 2316 54-55 -2.9755502448251576 2316 56-57 -2.980385538616826 2316 58-59 -3.0555020265096218 2316 60-61 -2.9142227533527247 2316 62-63 -2.8410707292677877 2316 64-65 -2.7970112598755676 2316 66-67 -2.857976705265095 2316 68-69 -2.7114525928027753 2316 70-71 -2.7071508162552114 2316 72-73 -2.729783860528503 2316 74-75 -3.067824102146904 2316 76-77 -3.319333813779707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97482.0 3 7834.0 4 9475.0 5 11549.0 6 13323.0 7 14075.0 8 15657.0 9 16107.0 10 18039.0 11 19566.0 12 17225.0 13 16078.0 14 16559.0 15 17401.0 16 17898.0 17 18898.0 18 20030.0 19 21255.0 20 22968.0 21 24555.0 22 27344.0 23 31265.0 24 36090.0 25 42072.0 26 49582.0 27 58759.0 28 69027.0 29 82113.0 30 96972.0 31 116310.0 32 141618.0 33 177517.0 34 235038.0 35 315276.0 36 453446.0 37 624438.0 38 564384.0 39 139198.0 40 938.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.96013394832373 26.725377382324073 27.86876240570711 22.44572626364509 2 22.66071370780552 26.865686660315564 27.807637152707425 22.66596247917149 3 22.952244605787914 27.097824691642753 27.406181940023455 22.54374876254588 4 22.47573410997962 27.003753131576108 27.760363133635035 22.760149624809237 5 22.83505470072685 27.010042631890286 27.56856799004576 22.586334677337106 6 22.965703232768934 26.95003981671257 27.608103462530952 22.47615348798754 7 22.930317948160035 26.60461827181984 27.626567302200343 22.838496477819785 8 22.62171341349147 27.014844223639518 27.64019627655509 22.72324608631392 9 22.72541705367743 26.618244423985193 27.76044181103358 22.8958967113038 10-11 23.06398879282571 26.87575809377944 27.4482748678899 22.61197824550495 12-13 23.10493395584799 26.6304664902443 27.421851232669592 22.842748321238123 14-15 22.8226075446868 26.91270441407813 27.6020450552336 22.66264298600147 16-17 23.098054620841495 26.667898599173085 27.578508842243153 22.655537937742267 18-19 22.98793837283618 26.64965528495084 27.430805422972842 22.93160091924013 20-21 22.927935560038108 26.927353015646293 27.376880385034102 22.767831039281496 22-23 23.001645164036372 26.82804540968229 27.12484942900651 23.045459997274833 24-25 22.97928538537006 26.97012416148364 27.105698416165225 22.944892036981077 26-27 23.070731548114424 26.911848696986013 27.066056363309997 22.951363391589563 28-29 23.17522146372267 26.8796523971059 27.098060017630885 22.847066121540536 30-31 22.988571078914628 26.73308268120721 27.181931290495843 23.09641494938232 32-33 22.947223995632314 27.095888566842145 27.142924161732278 22.81396327579326 34-35 23.061473365639166 26.813653846049245 27.178263894290417 22.94660889402117 36-37 23.097740618887258 26.774186310724563 27.41122694368579 22.71684612670239 38-39 23.00480522421093 26.825920604668145 27.35712668398732 22.8121474871336 40-41 22.896235283590848 26.781148364358415 27.251273868503834 23.0713424835469 42-43 22.756112522884948 27.10718633905173 27.05149957850766 23.085201559555664 44-45 22.74813547285752 26.85894134649029 27.31297170738235 23.07995147326984 46-47 22.78303830415679 26.789873135414243 27.318205863594393 23.108882696834574 48-49 22.87146794891844 26.77162474009788 27.232275321503508 23.124631989480168 50-51 22.80302121558557 26.916432674237655 27.129235902177157 23.151310207999618 52-53 22.860129605284097 26.8471527558464 27.156300229664065 23.13641740920544 54-55 22.863924986709392 26.81313602986127 27.20793065410461 23.11500832932473 56-57 22.772192268443874 27.01113693083288 27.15812712006388 23.058543680659362 58-59 22.83560530215812 27.045676269396456 27.121639744917204 22.99707868352822 60-61 22.888156092816445 26.858895081450886 26.994277100814795 23.25867172491788 62-63 22.86698122984283 26.740979882336653 27.253434794328506 23.138604093492013 64-65 22.824018511555103 26.78554842113924 27.151971680530163 23.238461386775498 66-67 22.815566790770202 26.83329006459576 27.31366144771801 23.037481696916025 68-69 22.843264113051845 26.664029979890273 27.357493109298375 23.135212797759504 70-71 22.745906657256246 26.64149995728307 27.316223344773487 23.296370040687194 72-73 22.83255157400636 26.53236959812242 27.254028129730095 23.381050698141127 74-75 22.699311037015026 26.495778482452614 27.231074200954254 23.57383627957811 76-77 22.58315146568835 26.398881838848236 27.563782714575137 23.45418398088828 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4863.0 1 2956.0 2 1016.5 3 1010.5 4 1037.0 5 1081.0 6 1173.0 7 1308.0 8 1447.0 9 1499.0 10 1582.5 11 1706.5 12 1832.5 13 1918.0 14 2049.5 15 2354.0 16 2781.0 17 3035.0 18 3272.0 19 4017.0 20 5185.5 21 6793.5 22 7741.0 23 9352.0 24 13128.0 25 18476.5 26 21660.0 27 25706.5 28 35457.0 29 46028.5 30 50896.0 31 58504.5 32 73149.0 33 86901.0 34 99923.0 35 106229.0 36 114256.5 37 129164.0 38 139505.0 39 142966.0 40 147932.0 41 157688.0 42 169653.5 43 176829.0 44 177640.0 45 178073.0 46 180861.5 47 185780.5 48 187533.0 49 186633.5 50 183635.5 51 176717.5 52 171898.0 53 168054.0 54 156637.5 55 138719.0 56 118783.5 57 109194.0 58 102713.5 59 86489.5 60 69913.5 61 63081.0 62 55320.5 63 40251.0 64 28747.5 65 24553.0 66 20961.5 67 15469.0 68 11422.0 69 7823.5 70 6371.0 71 5405.5 72 3741.5 73 2667.5 74 2292.0 75 1930.0 76 1285.5 77 827.0 78 651.0 79 528.5 80 327.0 81 215.0 82 162.0 83 142.0 84 122.5 85 92.5 86 76.0 87 70.0 88 57.0 89 50.0 90 48.0 91 40.0 92 36.0 93 40.5 94 38.0 95 30.5 96 34.0 97 36.0 98 36.5 99 78.0 100 121.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003317596504667342 2 0.008429958331531769 3 0.01242739018551619 4 0.018981002952932823 5 0.014575669889358158 6 0.013950221367986444 7 0.017974846635943547 8 0.01936171074855039 9 0.018382747845533794 10-11 0.02141481350348796 12-13 0.0208709452240343 14-15 0.008062847242900548 16-17 0.005751407055222481 18-19 0.011176493142772765 20-21 0.010551044621401053 22-23 0.014249348921685959 24-25 0.021156476070747474 26-27 0.013623900400314248 28-29 0.007926880173037132 30-31 0.007777316396187374 32-33 0.009748838909206902 34-35 0.01139404045455423 36-37 0.009748838909206902 38-39 0.01722702775169476 40-41 0.012794501274147411 42-43 0.014806813908125965 44-45 0.016982287025940613 46-47 0.016479208867445975 48-49 0.01348793333045083 50-51 0.026105677413775803 52-53 0.03051101047735047 54-55 0.024270121970619694 56-57 0.01590814717401963 58-59 0.016152887899773778 60-61 0.019796805372113317 62-63 0.025561809134322138 64-65 0.02008233621882649 66-67 0.01975601525115429 68-69 0.02800921639186362 70-71 0.05446840818728431 72-73 0.06590323876279756 74-75 0.07758281006406496 76-77 0.07495864561570104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3677361.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.91225751958962 #Duplication Level Percentage of deduplicated Percentage of total 1 74.65330107105297 40.99381293101116 2 13.841039119004556 15.200854088829843 3 4.86941833015768 8.021722599486857 4 2.2651209222272057 4.975316135774026 5 1.2174105625855347 3.342538115988268 6 0.741320681472085 2.442455529935568 7 0.5018528582109285 1.9290511370914425 8 0.3385881074311826 1.487410987866526 9 0.2506432726685642 1.2387049140896025 >10 1.1865837812082904 11.562668040439078 >50 0.0858521458101779 3.2680831220463906 >100 0.04678247120902404 4.582187163261688 >500 0.0018382630412929995 0.6849949167209647 >1k 2.484139205560893E-4 0.2702003174585479 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4916 0.13368282308971027 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.7193413972683128E-5 0.0 3 0.0 0.0 0.0 1.3596706986341564E-4 0.0 4 0.0 0.0 0.0 7.070287632897613E-4 0.0 5 0.0 0.0 0.0 0.001468444354524889 0.0 6 0.0 0.0 0.0 0.0030456623649405103 0.0 7 0.0 0.0 0.0 0.006444839111525901 0.0 8 0.0 0.0 0.0 0.011230879970718132 0.0 9 0.0 0.0 0.0 0.015799373518128896 0.0 10 0.0 0.0 0.0 0.02104770241485674 0.0 11 0.0 0.0 0.0 0.02635041813952995 0.0 12 0.0 0.0 0.0 0.03524266450859733 0.0 13 0.0 0.0 0.0 0.047697248108086204 0.0 14 0.0 0.0 0.0 0.05987989756784825 0.0 15 0.0 0.0 0.0 0.07423802014542494 0.0 16 2.7193413972683128E-5 0.0 0.0 0.08875930320683773 0.0 17 2.7193413972683128E-5 0.0 0.0 0.1031446191983871 0.0 18 2.7193413972683128E-5 0.0 0.0 0.11888960588857063 0.0 19 2.7193413972683128E-5 0.0 0.0 0.13580390937957953 0.0 20 2.7193413972683128E-5 0.0 0.0 0.15527439378402066 0.0 21 2.7193413972683128E-5 0.0 0.0 0.17547910036572423 0.0 22 2.7193413972683128E-5 0.0 0.0 0.19813121420496926 0.0 23 2.7193413972683128E-5 0.0 0.0 0.22203422508695775 0.0 24 2.7193413972683128E-5 0.0 0.0 0.24599162279689157 0.0 25 2.7193413972683128E-5 0.0 0.0 0.2701665678186069 0.0 26 2.7193413972683128E-5 0.0 0.0 0.2954292493992295 0.0 27 2.7193413972683128E-5 0.0 0.0 0.3201480627003985 0.0 28 2.7193413972683128E-5 0.0 0.0 0.3461449664582835 0.0 29 2.7193413972683128E-5 0.0 0.0 0.37448050381781933 0.0 30 2.7193413972683128E-5 0.0 0.0 0.4039853579781805 0.0 31 2.7193413972683128E-5 0.0 0.0 0.4365630679174549 0.0 32 2.7193413972683128E-5 0.0 0.0 0.4722136336356425 0.0 33 2.7193413972683128E-5 0.0 0.0 0.511562503654115 0.0 34 2.7193413972683128E-5 0.0 0.0 0.5507482131887514 0.0 35 2.7193413972683128E-5 0.0 0.0 0.5921365892551751 0.0 36 2.7193413972683128E-5 0.0 0.0 0.636597821100512 0.0 37 2.7193413972683128E-5 0.0 0.0 0.6807327319781767 0.0 38 2.7193413972683128E-5 0.0 0.0 0.7251667704095409 0.0 39 2.7193413972683128E-5 0.0 0.0 0.7711780268513209 0.0 40 2.7193413972683128E-5 0.0 0.0 0.8204796863837953 0.0 41 2.7193413972683128E-5 0.0 0.0 0.8750840616409431 0.0 42 2.7193413972683128E-5 0.0 0.0 0.9331420004726215 0.0 43 2.7193413972683128E-5 0.0 0.0 0.9993035766681596 0.0 44 2.7193413972683128E-5 0.0 0.0 1.0669335972182226 0.0 45 2.7193413972683128E-5 0.0 0.0 1.137935601100898 0.0 46 2.7193413972683128E-5 0.0 0.0 1.2177482711107233 0.0 47 2.7193413972683128E-5 0.0 0.0 1.2950591470350612 2.7193413972683128E-5 48 2.7193413972683128E-5 0.0 0.0 1.3722884427174813 2.7193413972683128E-5 49 2.7193413972683128E-5 0.0 0.0 1.4552011619201923 2.7193413972683128E-5 50 2.7193413972683128E-5 0.0 0.0 1.5398542596171547 2.7193413972683128E-5 51 2.7193413972683128E-5 0.0 0.0 1.6322030934683867 2.7193413972683128E-5 52 2.7193413972683128E-5 0.0 0.0 1.730860799361281 2.7193413972683128E-5 53 2.7193413972683128E-5 0.0 0.0 1.8359633443657013 2.7193413972683128E-5 54 2.7193413972683128E-5 0.0 0.0 1.9505835842605608 2.7193413972683128E-5 55 2.7193413972683128E-5 0.0 0.0 2.069092482353514 2.7193413972683128E-5 56 2.7193413972683128E-5 0.0 0.0 2.1921698739938775 2.7193413972683128E-5 57 2.7193413972683128E-5 0.0 0.0 2.3194622448000075 2.7193413972683128E-5 58 2.7193413972683128E-5 0.0 0.0 2.4466458419502466 2.7193413972683128E-5 59 2.7193413972683128E-5 0.0 0.0 2.582803265711471 2.7193413972683128E-5 60 2.7193413972683128E-5 0.0 0.0 2.7214080967302365 2.7193413972683128E-5 61 2.7193413972683128E-5 0.0 0.0 2.8721139969668465 2.7193413972683128E-5 62 2.7193413972683128E-5 0.0 0.0 3.025783979326479 2.7193413972683128E-5 63 2.7193413972683128E-5 0.0 0.0 3.188754109264769 2.7193413972683128E-5 64 5.4386827945366255E-5 0.0 0.0 3.3621665101685694 2.7193413972683128E-5 65 5.4386827945366255E-5 0.0 0.0 3.5431114867428026 2.7193413972683128E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCGCG 360 0.0 28.594023 56 CGCGGAA 380 0.0 25.224295 59 AATTCGC 425 0.0 25.057043 55 CTCGCGC 460 0.0 23.919933 36 CGATCGT 465 0.0 22.899418 1 CGCGCTT 510 0.0 22.272625 38 CCTCGCG 540 0.0 21.03268 35 TCGCGCT 530 0.0 20.761827 37 TCGCGGA 465 0.0 20.61256 58 CGGTTAC 425 0.0 20.043453 4 CAATTCG 570 0.0 19.929764 54 TCGCGTA 130 9.486104E-5 19.117147 61 GTCGGGT 485 0.0 19.039156 66 ATCGTTC 590 0.0 18.649187 3 GACGTAT 455 0.0 17.163849 39 CGTTCGG 145 2.1922021E-4 17.136248 11 CGCTCCG 650 0.0 16.929106 25 ACGCTCC 685 0.0 16.58389 24 GATCGTT 690 0.0 16.461031 2 ACTCGAG 695 0.0 16.342384 14 >>END_MODULE