FastQCFastQC Report
Thu 26 May 2016
SRR936040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677361
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58150.1581297022511524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41710.11342372968006134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12700.043.58789100-101
GGTATCA71300.036.055751
CGTACAT19000.033.990913100-101
CGGTATC9600.032.55178100-101
GTATCAA139600.031.7152291
CCTGTCG10000.029.16143696-97
ACGTATC5250.028.911297100-101
GCGTATC4150.027.251614100-101
CGAGTAC13450.026.553127100-101
TCGAGTA5150.025.42741100-101
TACGCAG10400.025.182917100-101
ATCAACG174550.024.7799473
CCGTACA10450.024.777624100-101
TCAACGC175100.024.7021144
TCGTATC5800.024.630312100-101
TATCAAC179550.024.1564922
TTATACG9750.024.1104596-97
CAACGCA181950.023.869265
TCGTACA11900.023.259087100-101
AACGCAG191500.022.7413566
Produced by FastQC (version 0.11.5)