FastQCFastQC Report
Thu 26 May 2016
SRR936039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420529
Sequences flagged as poor quality0
Sequence length77
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7545517.942876710048534No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6665915.851225480287923No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA18610.4425378511351179No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC17440.4147157508756828No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15780.3752416599093047No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG14940.35526681869740256No Hit
AGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGC11530.27417847520622834No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10550.2508744937923425No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG9070.2156807259428006No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC8690.206644488251702No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7750.18429168975266866No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG7290.1733530862318651No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6650.15813415959422536No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6030.14339082441401188No Hit
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT5860.1393482970258888No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA5700.13554356536647888No Hit
ACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACC5530.13150103797835583No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5400.12840969350508527No Hit
GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC5380.12793410204765904No Hit
TGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTC5140.12222700455854411No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4950.11770888571299482No Hit
AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG4950.11770888571299482No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4580.10891044375060935No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT4430.10534350781991254No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4320.1027277548040682No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCAT400.002247416435.5249466
TACGTAG601.10320325E-535.495362
CGACCAG400.002257940135.4911439
GGTAAAG400.002259258235.486925
ATACGTA751.332819E-633.1329461
GTAGGCT651.9114459E-532.7571565
CAAACCG450.004021554331.5476834
CTGGTCG450.004021554331.5476824
CGTAGGC703.171898E-530.4173584
ACGTAGG703.171898E-530.4173583
CAGTCAT500.00672744128.39628842
GAATACG500.006731350428.3929112
CTCATCC500.006731350428.3929160
GCAGGCA500.006731350428.3929160
CCGACCA500.006731350428.3929138
ATAATGC500.006735261528.38953427
GCTAGTG500.006735261528.38953432
CCGTCAC2100.027.06984368
CGTCACC2250.025.26518669
GTGGTAG3250.025.1257720
Produced by FastQC (version 0.11.5)