##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936039_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 420529 Sequences flagged as poor quality 0 Sequence length 77 %GC 24 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.820621170002546 40.0 35.0 41.0 31.0 41.0 2 36.5609696358634 39.0 35.0 41.0 31.0 41.0 3 36.36826711118615 39.0 35.0 41.0 31.0 41.0 4 36.16591959175229 39.0 35.0 41.0 31.0 41.0 5 35.94609408625802 38.0 35.0 40.0 31.0 41.0 6 35.548623281628615 37.0 35.0 40.0 30.0 41.0 7 35.04709544407164 37.0 35.0 40.0 30.0 41.0 8 34.51307757610058 35.0 35.0 40.0 29.0 41.0 9 34.20415714492936 35.0 34.0 39.0 29.0 41.0 10-11 34.016838315550174 35.0 34.0 39.0 27.5 41.0 12-13 33.61907502217445 35.0 33.5 39.0 24.5 41.0 14-15 33.21954966244896 35.0 33.0 39.0 21.5 41.0 16-17 32.74150177514511 35.0 33.0 39.0 20.0 41.0 18-19 32.24073488392001 35.0 33.0 39.0 16.5 41.0 20-21 31.550066701701905 35.0 33.0 38.0 4.5 41.0 22-23 30.66690882198374 35.0 31.5 38.0 2.0 41.0 24-25 30.057434802356077 35.0 31.0 38.0 2.0 41.0 26-27 29.09566522166129 34.0 27.5 37.0 2.0 40.0 28-29 29.155963084591075 35.0 27.5 37.0 2.0 39.5 30-31 29.432133099025275 35.0 29.5 37.0 2.0 40.0 32-33 29.26497815846232 35.0 29.0 37.0 2.0 40.0 34-35 29.028888614102712 35.0 28.0 36.0 2.0 40.0 36-37 28.750864981963193 35.0 27.0 36.0 2.0 40.0 38-39 28.41679170758735 35.0 27.0 35.0 2.0 40.0 40-41 28.044370305020582 35.0 26.0 35.0 2.0 39.5 42-43 27.714140998599383 35.0 26.0 35.0 2.0 39.0 44-45 27.389052835832963 35.0 25.5 35.0 2.0 39.0 46-47 27.04199829262667 35.0 24.0 35.0 2.0 38.0 48-49 26.623727495606722 34.0 23.0 35.0 2.0 37.0 50-51 26.22564674493317 34.0 20.0 35.0 2.0 37.0 52-53 25.781840253585365 33.5 14.5 35.0 2.0 36.0 54-55 25.330473047043128 33.0 4.0 35.0 2.0 36.0 56-57 24.873725712138757 33.0 2.0 35.0 2.0 35.0 58-59 24.40003899849951 33.0 2.0 35.0 2.0 35.0 60-61 24.002976013544846 33.0 2.0 35.0 2.0 35.0 62-63 23.549950181794834 33.0 2.0 35.0 2.0 35.0 64-65 23.066177362322218 33.0 2.0 35.0 2.0 35.0 66-67 22.559340735121715 32.0 2.0 35.0 2.0 35.0 68-69 22.070152117927655 31.5 2.0 35.0 2.0 35.0 70-71 21.568407886257546 31.0 2.0 35.0 2.0 35.0 72-73 21.005300466793017 31.0 2.0 35.0 2.0 35.0 74-75 20.446529252441568 30.0 2.0 35.0 2.0 35.0 76-77 19.66365220947901 28.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.1618917946746876 1101 2 0.9679432057652377 1101 3 0.7909877666391907 1101 4 1.1628557181334074 1101 5 0.9048421420657036 1101 6 0.8727352710036982 1101 7 1.0069735406701525 1101 8 1.0216347566947803 1101 9 1.2348187282520655 1101 10-11 1.3072042543178952 1101 12-13 1.5214366974032458 1101 14-15 1.516890458780189 1101 16-17 1.2062433793037002 1101 18-19 0.8673283613866616 1101 20-21 0.6292952686859401 1101 22-23 0.45444930285458085 1101 24-25 0.606963144298085 1101 26-27 0.20280735610706557 1101 28-29 -0.08225036780503103 1101 30-31 -0.05238978928354143 1101 32-33 -0.13200519093887308 1101 34-35 -0.3441192019408028 1101 36-37 -0.09289608553194384 1101 38-39 -0.2672255435489923 1101 40-41 -0.1372657522591929 1101 42-43 -0.2524036615819263 1101 44-45 -0.4125337418011341 1101 46-47 -0.5183815196728254 1101 48-49 -0.3443925695455441 1101 50-51 -0.44017164382392693 1101 52-53 -0.6554218615032745 1101 54-55 -0.5709730382548308 1101 56-57 -0.28332156206253245 1101 58-59 -0.3356924283013001 1101 60-61 -0.2635178760346726 1101 62-63 -0.6412693906850215 1101 64-65 -0.5019164717305102 1101 66-67 -0.6772076752658016 1101 68-69 -0.5592643561560671 1101 70-71 -0.31410601677607985 1101 72-73 -0.42158268906164054 1101 74-75 -0.4264337353407548 1101 76-77 -0.22846853651242682 1102 1 0.8394423926141457 1102 2 1.2304871953192134 1102 3 0.9784589047146355 1102 4 0.8917685989117601 1102 5 0.7208503587570476 1102 6 0.5465119234981586 1102 7 0.2814205860052681 1102 8 0.36063697010582274 1102 9 0.5306435025319161 1102 10-11 0.533250847416511 1102 12-13 0.5994833223494993 1102 14-15 0.91334979730469 1102 16-17 0.8167069217167935 1102 18-19 1.011399126844701 1102 20-21 1.2193564161564403 1102 22-23 1.177303169954378 1102 24-25 1.5721775585403464 1102 26-27 1.2227399983465936 1102 28-29 0.9350070726000723 1102 30-31 1.0860479246927959 1102 32-33 1.3102868837756922 1102 34-35 1.2338836324992357 1102 36-37 1.1976340184506036 1102 38-39 1.1111204492215947 1102 40-41 1.1054053093819114 1102 42-43 1.0611721915367518 1102 44-45 1.268743030121982 1102 46-47 1.0842275983067609 1102 48-49 1.2463970927102217 1102 50-51 1.3766486877100164 1102 52-53 1.1884649838286556 1102 54-55 1.2175068738456787 1102 56-57 1.4505953792900357 1102 58-59 1.3481839073091386 1102 60-61 1.2777924951495834 1102 62-63 1.3211304882939459 1102 64-65 1.7044283745250084 1102 66-67 1.811701383795647 1102 68-69 1.3200211420206536 1102 70-71 1.5391997683478849 1102 72-73 1.664632855771874 1102 74-75 1.9095190664554487 1102 76-77 1.7925270207416695 1103 1 0.2978905101531666 1103 2 0.08693806767917067 1103 3 0.042577362014910136 1103 4 0.1699366429889011 1103 5 0.14878883442279545 1103 6 0.025207974921485743 1103 7 0.3621597962899301 1103 8 0.2039565293344765 1103 9 -0.09093592813842832 1103 10-11 0.43943771610337734 1103 12-13 0.2572427346543691 1103 14-15 0.7094241774699768 1103 16-17 0.5717459483467238 1103 18-19 0.12642437936995776 1103 20-21 -0.009970948398198232 1103 22-23 -0.008452110119083756 1103 24-25 -0.21220031106479453 1103 26-27 -0.6601341981639663 1103 28-29 -0.6498643690895491 1103 30-31 -0.37730147842933803 1103 32-33 -0.7720822622298193 1103 34-35 -0.8428266521655949 1103 36-37 -0.8509885710810785 1103 38-39 -0.857543462624136 1103 40-41 -0.7998058935565631 1103 42-43 -0.911130379629455 1103 44-45 -0.8713166576649769 1103 46-47 -1.1934698305270324 1103 48-49 -1.0929394545808684 1103 50-51 -0.9149950221155088 1103 52-53 -0.8571158242521513 1103 54-55 -0.7900000132066616 1103 56-57 -0.8047650431158395 1103 58-59 -1.044114072488842 1103 60-61 -0.7060114534270951 1103 62-63 -0.7284562885655603 1103 64-65 -0.901402663124216 1103 66-67 -0.5141135840647735 1103 68-69 -0.5891615944348096 1103 70-71 -0.5321615723316384 1103 72-73 -0.9423919376165557 1103 74-75 -1.0765461309733837 1103 76-77 -0.7067039250801876 1104 1 0.0907601514480092 1104 2 0.09680245963017597 1104 3 0.2081285431172759 1104 4 0.16777128865818725 1104 5 0.40760335760739963 1104 6 0.3864503196283948 1104 7 0.4851038032890571 1104 8 0.32239484954444464 1104 9 0.5427473614503739 1104 10-11 0.6254722742836094 1104 12-13 0.8258341607261102 1104 14-15 0.944791631538223 1104 16-17 1.2159607339985214 1104 18-19 1.4918333907435368 1104 20-21 1.3698737585134388 1104 22-23 1.225154626538835 1104 24-25 1.6581518324172677 1104 26-27 1.3500582777765402 1104 28-29 1.5303412302105386 1104 30-31 1.447818819033479 1104 32-33 1.6552143256966936 1104 34-35 1.5559156925413191 1104 36-37 1.2698775706512038 1104 38-39 1.5988213667721922 1104 40-41 1.6073900819465017 1104 42-43 1.3529662957861177 1104 44-45 1.2465092390979287 1104 46-47 1.203194648913037 1104 48-49 1.39591662590907 1104 50-51 1.375206202731384 1104 52-53 1.5033063104810047 1104 54-55 1.2259448686831007 1104 56-57 1.2316413435858244 1104 58-59 1.1588496195496525 1104 60-61 1.0324290540094765 1104 62-63 1.1665568347940187 1104 64-65 1.027512472483835 1104 66-67 1.0394187868888558 1104 68-69 0.6686686767812908 1104 70-71 0.7272325659010832 1104 72-73 0.6690253852180881 1104 74-75 0.3278939390178657 1104 76-77 0.10050298655585976 1105 1 0.3107631388794587 1105 2 0.3398513468119617 1105 3 0.3357345435867316 1105 4 0.38423203594282995 1105 5 0.14872108374529347 1105 6 0.3252079749214829 1105 7 0.2946123708156776 1105 8 0.38993213909056834 1105 9 0.28819686318949067 1105 10-11 0.2772764694909142 1105 12-13 0.7306844690717114 1105 14-15 0.6107114403425982 1105 16-17 0.5589071949591968 1105 18-19 1.0295070351965165 1105 20-21 1.0245480217915066 1105 22-23 0.8744408438104507 1105 24-25 0.6893918298566177 1105 26-27 0.6161530647086586 1105 28-29 0.7198510780649201 1105 30-31 0.5944803643890921 1105 32-33 0.6132835914287185 1105 34-35 0.6559199603005297 1105 36-37 0.8033474722793557 1105 38-39 1.0594619574300665 1105 40-41 1.1223485779881521 1105 42-43 1.097914335817702 1105 44-45 1.2019218247198467 1105 46-47 1.0312252914241853 1105 48-49 1.1876499763134412 1105 50-51 1.273622864063352 1105 52-53 1.5768611405174973 1105 54-55 1.3121002577960468 1105 56-57 1.553195661491209 1105 58-59 1.4415566592184774 1105 60-61 1.416922150908949 1105 62-63 1.4720857168544974 1105 64-65 1.7930417813202268 1105 66-67 2.0043484755558225 1105 68-69 1.8799440966221006 1105 70-71 1.4440240645247329 1105 72-73 1.553678523088049 1105 74-75 1.5733861183491094 1105 76-77 1.4549830667897332 1106 1 -0.5180817120690548 1106 2 -0.2389299878763822 1106 3 -0.2990893046517513 1106 4 -0.20975085701110174 1106 5 -0.5510375544660917 1106 6 -0.40222258063406713 1106 7 -0.1182221481545156 1106 8 -0.2752101373321949 1106 9 -0.26003315036064834 1106 10-11 0.013482854992268756 1106 12-13 -0.0048232375678551875 1106 14-15 0.15135126080330963 1106 16-17 0.5023883094578352 1106 18-19 0.44144174048106777 1106 20-21 -0.12984942062042037 1106 22-23 -0.050292387896863744 1106 24-25 -0.32591558884256955 1106 26-27 -0.17480433705285847 1106 28-29 0.12348632535489656 1106 30-31 0.060372132681774104 1106 32-33 -0.11010309556315079 1106 34-35 -0.11166345772114994 1106 36-37 -0.1824989877477421 1106 38-39 -0.060668462624136055 1106 40-41 -0.027062838001004508 1106 42-43 0.16326198148165716 1106 44-45 -0.15846943544275405 1106 46-47 0.00817947502851979 1106 48-49 -0.004571399025312672 1106 50-51 -0.21594988322662445 1106 52-53 0.009724453525624455 1106 54-55 0.21989582012666986 1106 56-57 0.13160648466193692 1106 58-59 0.19607689973338083 1106 60-61 0.43843299101735056 1106 62-63 0.3145124614344432 1106 64-65 0.07602789243134112 1106 66-67 0.33788294371300154 1106 68-69 -0.1275296499903611 1106 70-71 -0.8336372667760799 1106 72-73 -0.4337981876165564 1106 74-75 -0.5100530754178294 1106 76-77 -0.657832397302407 1107 1 -0.014695649207183692 1107 2 -0.04733957224362939 1107 3 -0.17150452384116477 1107 4 -0.10295417586966238 1107 5 -0.3422712703469841 1107 6 -0.3130605665728652 1107 7 -0.35682972673185276 1107 8 -0.1619450422122597 1107 9 -0.36452291462863684 1107 10-11 -0.3204223610954102 1107 12-13 -0.5669645992303813 1107 14-15 -0.4224410003629444 1107 16-17 -0.1738323532799626 1107 18-19 -0.3077602084436606 1107 20-21 -0.3986633939454798 1107 22-23 -0.11560333702837866 1107 24-25 -0.6457369804885609 1107 26-27 -1.214076850493715 1107 28-29 -0.9941502803109472 1107 30-31 -1.0022635683107808 1107 32-33 -1.1614950000296496 1107 34-35 -1.2023214136764864 1107 36-37 -0.989574179021524 1107 38-39 -1.1264640581576373 1107 40-41 -0.8209459542127533 1107 42-43 -0.9039584744736793 1107 44-45 -0.9774443113732758 1107 46-47 -1.2806665495785907 1107 48-49 -1.127923601258562 1107 50-51 -1.115353445054577 1107 52-53 -1.0254608752584957 1107 54-55 -1.3915922381859858 1107 56-57 -1.2336973563223452 1107 58-59 -1.0905332894725746 1107 60-61 -1.1926153960794252 1107 62-63 -1.1722114581271832 1107 64-65 -1.1045319710922321 1107 66-67 -0.9527853513655735 1107 68-69 -1.1599363394030995 1107 70-71 -1.0467362897289334 1107 72-73 -0.9452455356314466 1107 74-75 -1.5385050501903663 1107 76-77 -1.2546809032991 1108 1 -0.09875494198153945 1108 2 0.10793382477277902 1108 3 -0.2607042608967802 1108 4 0.03586743057839925 1108 5 -0.1894437298837559 1108 6 0.10935011228558267 1108 7 -0.07872458451091546 1108 8 0.011136322492788508 1108 9 -0.01745409308141177 1108 10-11 -0.09739932977304733 1108 12-13 -0.1112802035583087 1108 14-15 -0.18319714313463464 1108 16-17 -0.4132746032311019 1108 18-19 -0.08160730983714615 1108 20-21 -0.1436191849402313 1108 22-23 0.12465913358284908 1108 24-25 -0.11194507372721674 1108 26-27 -0.4604391570608115 1108 28-29 -0.36411125320516646 1108 30-31 -0.33310564178123414 1108 32-33 -0.4020743067803352 1108 34-35 -0.40933089507198517 1108 36-37 -0.2043298598638934 1108 38-39 -0.1639277108341588 1108 40-41 -0.28635455438923074 1108 42-43 -0.5071493401173903 1108 44-45 -0.5183665118150671 1108 46-47 -0.43814299414411195 1108 48-49 -0.5638457128677921 1108 50-51 -0.6865675492966297 1108 52-53 -0.6067545142548063 1108 54-55 -0.6072036727134247 1108 56-57 -0.7808556026491189 1108 58-59 -0.792446742868048 1108 60-61 -1.003840278672385 1108 62-63 -0.9973504810874338 1108 64-65 -0.963388873678447 1108 66-67 -0.588115838108795 1108 68-69 -0.31313401554326603 1108 70-71 -0.11780530078562634 1108 72-73 -0.2931079280700182 1108 74-75 -0.3994909731903 1108 76-77 -0.32999018867074525 1109 1 0.2451260231781589 1109 2 0.02336815805663406 1109 3 0.23958693139290688 1109 4 0.4023529109314836 1109 5 0.42006740964873757 1109 6 0.31191717215699555 1109 7 0.21313002489173982 1109 8 0.5434700859532953 1109 9 0.30358826112260573 1109 10-11 0.301990879314431 1109 12-13 0.42832194783884603 1109 14-15 0.48928861128177914 1109 16-17 0.4333155921532139 1109 18-19 0.3136319817091433 1109 20-21 -0.3446931706204204 1109 22-23 -0.18557199316002126 1109 24-25 0.07232254911595959 1109 26-27 0.34627196876851585 1109 28-29 0.2970122922607956 1109 30-31 0.3430799676100058 1109 32-33 0.3632446915181866 1109 34-35 0.30395032816401724 1109 36-37 0.3030784145648582 1109 38-39 0.4050163459883045 1109 40-41 0.27899527603408103 1109 42-43 -0.036442713494420076 1109 44-45 -0.32361596654322966 1109 46-47 -0.4939948172362314 1109 48-49 -0.1006016023745957 1109 50-51 -0.03679193864926944 1109 52-53 -0.10021125221600258 1109 54-55 -0.167551847337446 1109 56-57 -0.28055584588032545 1109 58-59 -0.003889457922120698 1109 60-61 0.04997605321830889 1109 62-63 -0.09169392811898902 1109 64-65 -0.14010548077440177 1109 66-67 0.19059800551523765 1109 68-69 0.21501171763324223 1109 70-71 0.3425925478172225 1109 72-73 0.044689402096363295 1109 74-75 -0.040567927889917144 1109 76-77 -0.08524592401692033 1110 1 -0.4665589168523141 1110 2 -0.5862183847373714 1110 3 -0.29049438686401174 1110 4 0.0018873156346401743 1110 5 0.07301653746215919 1110 6 0.04170025195188032 1110 7 -0.02686611302745945 1110 8 -0.07836783538899539 1110 9 0.09850104246446278 1110 10-11 -0.15797758035900245 1110 12-13 -0.004665586222564855 1110 14-15 0.09219697268671467 1110 16-17 -0.02900766401002386 1110 18-19 0.18482622291754325 1110 20-21 0.12279561861724986 1110 22-23 0.5602387219686058 1110 24-25 0.4079105509182668 1110 26-27 0.410543430136979 1110 28-29 0.4319866523354605 1110 30-31 0.7231033546548673 1110 32-33 0.4445844044368492 1110 34-35 0.7619877474357999 1110 36-37 0.6889217428202699 1110 38-39 0.711402725716674 1110 40-41 0.7124387501903229 1110 42-43 0.44712198708704065 1110 44-45 0.48285623720298787 1110 46-47 0.20051995522284116 1110 48-49 0.5164838139791748 1110 50-51 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72-73 -0.34347675023983015 2313 74-75 -0.3435250921831141 2313 76-77 -0.3717515910893887 2314 1 -0.6528985706742958 2314 2 -0.4572451270318538 2314 3 -0.7048794518046648 2314 4 -0.6905719942471862 2314 5 -0.49485053431254045 2314 6 -0.5425676702054076 2314 7 -0.49429680426455747 2314 8 -0.5523614489124853 2314 9 -0.8024095898999946 2314 10-11 -0.6077658454236001 2314 12-13 -0.7676666276830204 2314 14-15 -0.6661196485057133 2314 16-17 -0.9252123583284693 2314 18-19 -0.4240687961472105 2314 20-21 -0.2655185065129366 2314 22-23 -0.4789602141924476 2314 24-25 -0.4659457791816628 2314 26-27 -0.1896820761763358 2314 28-29 -0.3308357915676936 2314 30-31 -0.4115118644391451 2314 32-33 -0.23727739978579976 2314 34-35 -0.10310869524514032 2314 36-37 0.10112275329512244 2314 38-39 -0.03532657202912759 2314 40-41 0.21315529219733165 2314 42-43 0.2729893687177416 2314 44-45 0.45210518068008554 2314 46-47 0.43766953820830423 2314 48-49 0.5141276172894642 2314 50-51 0.36941529991477395 2314 52-53 0.24069554277706473 2314 54-55 -0.006426756648760801 2314 56-57 0.12167716204517376 2314 58-59 -0.034151927042049834 2314 60-61 0.14008846126687047 2314 62-63 -0.14048443955724466 2314 64-65 -0.21484902695445385 2314 66-67 -0.01948519848149388 2314 68-69 -0.11163973476323719 2314 70-71 -0.0727432528605334 2314 72-73 0.09014165163488386 2314 74-75 0.2741309856826213 2314 76-77 0.40337551616528344 2315 1 -0.5343317120690543 2315 2 -0.5645698688287624 2315 3 -0.7100416856041321 2315 4 -0.8045871665349082 2315 5 -0.7555762449422829 2315 6 -0.9770142473007368 2315 7 -1.0244275052973677 2315 8 -0.8548529944750527 2315 9 -0.7445867217892186 2315 10-11 -0.6025067283410692 2315 12-13 -0.595455678044047 2315 14-15 -1.0831948701490717 2315 16-17 -1.0584524643516815 2315 18-19 -0.8097264142808349 2315 20-21 -0.8299312658585158 2315 22-23 -0.801587030754007 2315 24-25 -0.9367638031282866 2315 26-27 -1.0990230870528563 2315 28-29 -1.02422944845463 2315 30-31 -1.0453570339848923 2315 32-33 -0.9473203574679125 2315 34-35 -0.9789774458163905 2315 36-37 -1.129559999652507 2315 38-39 -1.134924415005088 2315 40-41 -1.0359170046676738 2315 42-43 -1.1558526018516773 2315 44-45 -1.0888563402046572 2315 46-47 -1.0625571321143354 2315 48-49 -0.8446458037872198 2315 50-51 -1.0956299427504312 2315 52-53 -1.0675523321886615 2315 54-55 -1.1673809655876148 2315 56-57 -1.3175825034333002 2315 58-59 -1.1008204216951896 2315 60-61 -1.129543199458837 2315 62-63 -1.4198848599941307 2315 64-65 -1.4882280599496127 2315 66-67 -1.212407234858425 2315 68-69 -1.0456695309427424 2315 70-71 -1.1726774453475102 2315 72-73 -2.227630032854652 2315 74-75 -2.454085813513064 2315 76-77 -2.3683309092071703 2316 1 -2.362550102873648 2316 2 -2.5931864533936206 2316 3 -2.0828536724678486 2316 4 -2.1248909432179985 2316 5 -2.587944882052298 2316 6 -2.108939534657061 2316 7 -2.4920547630970447 2316 8 -2.5582561143436884 2316 9 -2.3920374607054775 2316 10-11 -2.7062998317893445 2316 12-13 -2.992565694464407 2316 14-15 -2.8695269719553096 2316 16-17 -3.8677176531858386 2316 18-19 -3.9230966934269773 2316 20-21 -2.864089722344559 2316 22-23 -3.020928163758935 2316 24-25 -2.5937855601069373 2316 26-27 -2.408146650271245 2316 28-29 -2.4680636028060263 2316 30-31 -2.2243800224906387 2316 32-33 -2.440113871425222 2316 34-35 -2.398166690479769 2316 36-37 -2.4012040883224586 2316 38-39 -2.3866382902103425 2316 40-41 -2.1952560851274434 2316 42-43 -2.2280365098976525 2316 44-45 -2.2042668492358573 2316 46-47 -1.9455292175002157 2316 48-49 -1.8270390714391063 2316 50-51 -2.4328590067898404 2316 52-53 -3.0099414947502368 2316 54-55 -2.3159339212526397 2316 56-57 -2.1249039320047274 2316 58-59 -2.191719436473516 2316 60-61 -1.8676014917412687 2316 62-63 -2.0165310012092377 2316 64-65 -2.1184764179134845 2316 66-67 -1.9353300591605596 2316 68-69 -1.6297746212547288 2316 70-71 -1.3378129133278023 2316 72-73 -1.1895902134786276 2316 74-75 -1.0823411501304712 2316 76-77 -1.0662824691414876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10836.0 3 1935.0 4 3504.0 5 4137.0 6 4145.0 7 4148.0 8 4956.0 9 5716.0 10 8530.0 11 10200.0 12 6518.0 13 3884.0 14 3612.0 15 3521.0 16 3689.0 17 3888.0 18 4105.0 19 4386.0 20 4401.0 21 4700.0 22 5216.0 23 5663.0 24 6474.0 25 7079.0 26 8087.0 27 8748.0 28 10188.0 29 10926.0 30 12660.0 31 14745.0 32 18085.0 33 24009.0 34 51029.0 35 57803.0 36 25816.0 37 25998.0 38 21191.0 39 5943.0 40 58.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 12.846379954909962 33.22369985635874 42.957059825157195 10.9728603635741 2 14.213754509802056 32.73789280167811 43.0386731133747 10.009679575145135 3 11.869405150353408 34.24047983713696 42.69351877396094 11.196596238548691 4 12.332448604887887 34.49489616332278 43.401400344378175 9.771254887411171 5 11.568207762557076 34.492009132420094 43.21632420091324 10.72345890410959 6 11.082837084530356 36.67738437244344 43.16056581876296 9.079212724263238 7 12.68616249589741 35.97936575133304 41.4528166367792 9.881655115990354 8 10.873887543820429 36.736145134208236 41.756765112993676 10.633202208977659 9 10.809698785868028 37.053262459419926 41.80324164912656 10.333797105585482 10-11 10.59081373943404 37.043648056624505 41.87318945711065 10.492348746830807 12-13 11.331818349380987 37.37598595845329 40.903258642484005 10.388937049681722 14-15 11.951048111211918 37.38515010303345 40.621339874837844 10.042461910916789 16-17 10.69025420655427 38.381634441486355 40.669005920269576 10.259105431689791 18-19 10.712799229406238 38.64135282015911 40.026756727830566 10.619091222604084 20-21 10.975402359270884 38.82652496928714 39.001823148635964 11.196249522806008 22-23 10.724755225794022 39.29623476441858 38.13922844839376 11.839781561393641 24-25 10.98238537172431 39.77324079667781 37.069326381984844 12.17504744961303 26-27 11.426427337131875 40.232431744581966 36.129792626262265 12.211348292023896 28-29 10.609638640174081 40.65042390515939 35.818737440397626 12.921200014268896 30-31 10.657095396015881 40.5354885735723 35.436492034973114 13.370923995438705 32-33 11.396177056392878 40.500371592496805 34.512797645589934 13.590653705520378 34-35 11.080445391501224 39.724817115514504 35.44641413228565 13.748323360698622 36-37 11.733914888152167 39.819183698707675 35.28403118326111 13.162870229879042 38-39 12.482534488068751 39.68972926821437 35.371125034633735 12.456611209083137 40-41 11.936063850533435 39.88529830052178 36.098453679348935 12.080184169595846 42-43 11.969296081147771 39.76549911705423 35.77791386968077 12.487290932117228 44-45 12.312458008549905 39.762647973410864 35.524796508689626 12.4000975093496 46-47 11.467613322154236 39.92674123362529 35.626832212450424 12.978813231770042 48-49 11.583117190463788 39.3470404184193 35.539596065545695 13.530246325571218 50-51 11.384464603669814 39.43391087254133 35.957019379102725 13.22460514468612 52-53 10.8438392467382 39.33142781697052 37.030415704690164 12.794317231601122 54-55 10.714077583879526 39.09188541172357 37.15258575270064 13.041451251696262 56-57 10.249881671300711 39.710635787831286 36.71103299170629 13.328449549161709 58-59 10.186251052433798 39.369348371046534 35.82900864303824 14.615391933481428 60-61 10.7395184835025 38.70422233718047 35.77671144092236 14.779547738394669 62-63 10.957547012734944 38.429967318240415 35.99132292147339 14.621162747551248 64-65 11.318257229334776 37.71420416366869 36.5702358936546 14.397302713341936 66-67 11.06141065513817 38.06711345539745 36.382081670759916 14.48939421870447 68-69 10.728699431556636 38.40126939267316 36.14117473537469 14.728856440395518 70-71 10.752548744364212 38.27208812647958 35.9816085937593 14.993754535396913 72-73 11.640816695662108 37.50229067949288 35.44710493811 15.409787686735003 74-75 12.203295036026441 36.76505762445633 35.38825853359592 15.643388805921306 76-77 12.258158182220626 36.79827494698461 36.099495193509156 14.844071677285612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 121127.0 1 67876.5 2 12040.5 3 8417.5 4 7380.0 5 6674.0 6 5381.0 7 4598.0 8 3934.0 9 3466.0 10 3320.0 11 3046.0 12 2850.0 13 2782.0 14 2749.0 15 2734.0 16 2786.5 17 2821.0 18 2995.0 19 3295.5 20 3501.5 21 3880.5 22 4180.0 23 4275.5 24 4513.5 25 4841.5 26 5027.0 27 5404.0 28 5877.0 29 6138.0 30 6303.0 31 6544.0 32 7218.5 33 7973.5 34 8157.5 35 8020.0 36 8381.5 37 9039.0 38 8920.5 39 8506.0 40 8274.5 41 7734.0 42 7130.5 43 6836.0 44 6793.5 45 6831.5 46 7507.0 47 10723.0 48 13344.0 49 12252.0 50 12432.0 51 9986.5 52 6269.0 53 5645.0 54 4737.5 55 4315.5 56 4033.0 57 3889.0 58 4330.0 59 4458.5 60 3932.0 61 3718.0 62 3526.0 63 2482.5 64 1190.5 65 750.0 66 663.5 67 530.5 68 449.0 69 388.5 70 363.0 71 331.5 72 313.5 73 327.5 74 328.0 75 330.0 76 262.0 77 165.5 78 139.0 79 136.5 80 120.0 81 87.5 82 51.5 83 34.0 84 29.5 85 18.5 86 14.0 87 16.0 88 12.0 89 9.5 90 7.0 91 3.0 92 3.5 93 4.0 94 2.5 95 1.5 96 2.0 97 3.0 98 3.0 99 11.5 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00879844196238547 2 0.013316560807934768 3 0.012603173621795406 4 0.014505539451500373 5 0.011651990706942922 6 0.009749624877237954 7 0.014981130908926613 8 0.014981130908926613 9 0.015218926637639733 10-11 0.017834679653484065 12-13 0.014862233044570053 14-15 0.00820395264060267 16-17 0.005825995353471461 18-19 0.01759688392477094 20-21 0.024374062193094886 22-23 0.021520513448537438 24-25 0.019737045483189032 26-27 0.02484965365052113 28-29 0.00808505477624611 30-31 0.00820395264060267 32-33 0.009868522741594516 34-35 0.01070080779209044 36-37 0.01117639924951668 38-39 0.013673254401004448 40-41 0.01117639924951668 42-43 0.014624437315856933 44-45 0.013435458672291328 46-47 0.024136266464381766 48-49 0.021282717719824314 50-51 0.03650164435746405 52-53 0.04054417174558711 54-55 0.028416589581217944 56-57 0.02140161558418088 58-59 0.017834679653484065 60-61 0.020569330533684954 62-63 0.026395325887156415 64-65 0.025325245107947373 66-67 0.028892181038644184 68-69 0.04054417174558711 70-71 0.05326624323173907 72-73 0.08275291359216605 74-75 0.08358519864266198 76-77 0.08750882816642848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 420529.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.769557402444484 #Duplication Level Percentage of deduplicated Percentage of total 1 86.08114943201983 32.51246914741074 2 6.86029533922282 5.182206372249974 3 2.446422706886017 2.772009085751251 4 1.3033927940498442 1.9691427581119236 5 0.7577522227847173 1.4309983037648641 6 0.4680056815937844 1.0605820473375958 7 0.3460272670540568 0.9148507708066429 8 0.24154702616743093 0.7298499416176434 9 0.19919221912846613 0.6771061759043611 >10 1.1355314970322028 8.018291736653923 >50 0.08380763635331186 2.210052173311368 >100 0.06427352562434444 4.716888221285591 >500 0.0075615912499228735 1.8680486224769466 >1k 0.0037807956249614368 2.1146148567598 >5k 0.0 0.0 >10k+ 0.001260265208320479 33.82288978655737 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 75455 17.942876710048534 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66659 15.851225480287923 No Hit CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA 1861 0.4425378511351179 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 1744 0.4147157508756828 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1578 0.3752416599093047 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1494 0.35526681869740256 No Hit AGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGC 1153 0.27417847520622834 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1055 0.2508744937923425 No Hit AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG 907 0.2156807259428006 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 869 0.206644488251702 No Hit TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 775 0.18429168975266866 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 729 0.1733530862318651 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 665 0.15813415959422536 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 603 0.14339082441401188 No Hit TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT 586 0.1393482970258888 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 570 0.13554356536647888 No Hit ACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACC 553 0.13150103797835583 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 540 0.12840969350508527 No Hit GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 538 0.12793410204765904 No Hit TGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTC 514 0.12222700455854411 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 495 0.11770888571299482 No Hit AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG 495 0.11770888571299482 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 458 0.10891044375060935 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 443 0.10534350781991254 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 432 0.1027277548040682 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 9.511829148524834E-4 0.0 2 0.0 0.0 0.0 0.009036237691098592 0.0 3 0.0 0.0 0.0 0.013078765079221647 0.0 4 0.0 0.0 0.0 0.024255164328738327 0.0 5 0.0 0.0 0.0 0.03923629523766494 0.0 6 0.0 0.0 0.0 0.061589093736698304 0.0 7 0.0 0.0 0.0 0.0946427000278221 0.0 8 0.0 0.0 0.0 0.12365377893082284 0.0 9 0.0 0.0 0.0 0.15337824501996294 0.0 10 0.0 0.0 0.0 0.19023658297049667 0.0 11 0.0 0.0 0.0 0.2347043842398503 0.0 12 0.0 0.0 0.0 0.2782210025943514 0.0 13 0.0 0.0 0.0 0.32910928853895927 0.0 14 0.0 0.0 0.0 0.4082952662004285 0.0 15 0.0 0.0 0.0 0.4789205976282254 0.0 16 0.0 0.0 0.0 0.5773680293154574 0.0 17 0.0 0.0 0.0 0.6446642205412706 0.0 18 0.0 0.0 0.0 0.7055399270918296 0.0 19 0.0 0.0 0.0 0.7552392343928719 0.0 20 0.0 0.0 0.0 0.8163527366721439 0.0 21 0.0 0.0 0.0 0.8762772603078504 0.0 22 0.0 0.0 0.0 0.9397687198742536 0.0 23 0.0 0.0 0.0 1.0042113623555093 0.0 24 0.0 0.0 0.0 1.0767390596130113 0.0 25 0.0 0.0 0.0 1.1540226714447754 0.0 26 0.0 0.0 0.0 1.2308306918191136 0.0 27 0.0 0.0 0.0 1.3040717762627547 0.0 28 0.0 0.0 0.0 1.3782640436212485 0.0 29 0.0 0.0 0.0 1.4491271707777584 0.0 30 0.0 0.0 0.0 1.5145209961738668 0.0 31 0.0 0.0 0.0 1.5832439617719587 0.0 32 0.0 0.0 0.0 1.6579118205878787 0.0 33 0.0 0.0 0.0 1.729488334930528 0.0 34 0.0 0.0 0.0 1.8255578093306288 0.0 35 0.0 0.0 0.0 1.9235296495604346 0.0 36 0.0 0.0 0.0 2.0317267061249047 0.0 37 0.0 0.0 0.0 2.139923762689375 0.0 38 0.0 0.0 0.0 2.2302861396003606 0.0 39 0.0 0.0 0.0 2.3215996994261987 0.0 40 0.0 0.0 0.0 2.41386444216689 0.0 41 0.0 0.0 0.0 2.514214239683827 0.0 42 0.0 0.0 0.0 2.6131372628284852 0.0 43 0.0 0.0 0.0 2.728943782711775 0.0 44 0.0 0.0 0.0 2.853073153100024 0.0 45 0.0 0.0 0.0 2.9669773071536087 0.0 46 0.0 0.0 0.0 3.1412815763003263 0.0 47 0.0 0.0 0.0 3.279678690411363 0.0 48 0.0 0.0 0.0 3.4166490301501202 0.0 49 0.0 0.0 0.0 3.5640823819522556 0.0 50 0.0 0.0 0.0 3.68559599932466 0.0 51 0.0 0.0 0.0 3.821139564691139 0.0 52 0.0 0.0 0.0 3.9543051727704865 0.0 53 0.0 0.0 0.0 4.106018847689458 0.0 54 0.0 0.0 0.0 4.250123059289609 0.0 55 0.0 0.0 0.0 4.394702862347186 0.0 56 0.0 0.0 0.0 4.5580685279731 0.0 57 0.0 0.0 0.0 4.735226345864375 0.0 58 0.0 0.0 0.0 4.918091261244766 0.0 59 0.0 0.0 0.0 5.100718380896442 0.0 60 0.0 0.0 0.0 5.277400607330291 0.0 61 0.0 0.0 0.0 5.4624056842691 0.0 62 0.0 0.0 0.0 5.67047694689308 0.0 63 0.0 0.0 0.0 5.8338426125189935 0.0 64 0.0 0.0 0.0 6.050474521376647 0.0 65 0.0 0.0 0.0 6.234528415400603 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGCAT 40 0.0022474164 35.52494 66 TACGTAG 60 1.10320325E-5 35.49536 2 CGACCAG 40 0.0022579401 35.49114 39 GGTAAAG 40 0.0022592582 35.48692 5 ATACGTA 75 1.332819E-6 33.132946 1 GTAGGCT 65 1.9114459E-5 32.757156 5 CAAACCG 45 0.0040215543 31.54768 34 CTGGTCG 45 0.0040215543 31.54768 24 CGTAGGC 70 3.171898E-5 30.417358 4 ACGTAGG 70 3.171898E-5 30.417358 3 CAGTCAT 50 0.006727441 28.396288 42 GAATACG 50 0.0067313504 28.39291 12 CTCATCC 50 0.0067313504 28.39291 60 GCAGGCA 50 0.0067313504 28.39291 60 CCGACCA 50 0.0067313504 28.39291 38 ATAATGC 50 0.0067352615 28.389534 27 GCTAGTG 50 0.0067352615 28.389534 32 CCGTCAC 210 0.0 27.069843 68 CGTCACC 225 0.0 25.265186 69 GTGGTAG 325 0.0 25.12577 20 >>END_MODULE