FastQCFastQC Report
Thu 26 May 2016
SRR936038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420178
Sequences flagged as poor quality0
Sequence length77
%GC24

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7620218.135647273298456No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6659315.848759335329312No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC17350.4129202385655603No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA17270.4110162835750563No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15190.36151345382195166No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG15100.3593715044576346No Hit
AGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGC11830.2815473442207826No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10550.2510840643727182No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC8460.2013432402458006No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8120.19325143153615848No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG7860.1870635778170204No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG7430.17682981974306128No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT6960.16564408417385013No Hit
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT6420.15279238798794798No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6230.14827049488550093No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA5740.13660877056866375No Hit
ACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACC5730.13637077619485075No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5440.12946893935427367No Hit
GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC4940.11756922066362352No Hit
TGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTC4920.1170932319159975No Hit
AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG4740.11280933318736344No Hit
TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4590.10923941758016839No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4370.10400354135628234No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA4350.10352755260865634No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCGT252.226416E-456.80590454
CGTAGGC451.5279402E-647.326984
ACGTAGG503.1555155E-642.5942843
TACGTAG503.1555155E-642.5942842
ATACGTA556.0840357E-638.712861
AGTTGGC601.1032276E-535.49523523
TGTTAAG501.5731774E-435.49523518
ATGCTAC651.9083776E-532.76483519
CAGAACG450.00400991531.56635546
CTTCGAG450.004016935431.55507955
AGCAGCG450.004016935431.55507959
CTACGTA450.004019277631.55132162
TACGTAT450.004019277631.55132163
GCCTTGA604.5857934E-429.5793633
GAACGAC500.0067080128.41310348
CGTATTC500.0067158228.40633465
CGACTCG500.0067158228.40633451
GCTGAAC500.00672363728.39956939
GCCTACG500.00672363728.39956960
ACGTATT500.00672363728.39956964
Produced by FastQC (version 0.11.5)