##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936038_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 420178 Sequences flagged as poor quality 0 Sequence length 77 %GC 24 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.81667769373932 40.0 35.0 41.0 31.0 41.0 2 36.57281437866809 39.0 35.0 41.0 31.0 41.0 3 36.32890108477836 39.0 35.0 41.0 31.0 41.0 4 36.159644246010025 39.0 35.0 41.0 31.0 41.0 5 35.929303771258844 38.0 35.0 40.0 30.0 41.0 6 35.54160617642999 37.0 35.0 40.0 30.0 41.0 7 35.062280747683126 37.0 35.0 40.0 30.0 41.0 8 34.56033395370533 35.0 35.0 40.0 30.0 41.0 9 34.304935051335384 35.0 34.0 40.0 29.0 41.0 10-11 34.042654541646634 35.0 34.0 40.0 27.0 41.0 12-13 33.55996863234154 35.0 33.0 40.0 23.5 41.0 14-15 33.138685271480185 35.0 33.0 39.0 21.5 41.0 16-17 32.68357696024066 35.0 33.0 39.0 20.0 41.0 18-19 32.18466459452898 35.0 33.0 39.0 15.5 41.0 20-21 31.49372170841881 35.0 33.0 38.0 4.5 41.0 22-23 30.605945575446597 35.0 31.0 38.0 2.0 41.0 24-25 29.995419798275968 35.0 30.5 38.0 2.0 41.0 26-27 28.998974244248867 34.0 27.5 37.0 2.0 40.0 28-29 29.05031201062407 35.0 27.5 37.0 2.0 39.5 30-31 29.37922975500859 35.0 29.5 37.0 2.0 40.0 32-33 29.253828139502783 35.0 29.5 37.0 2.0 40.0 34-35 29.029879003660355 35.0 28.0 36.0 2.0 40.0 36-37 28.7249772715373 35.0 27.0 35.5 2.0 40.0 38-39 28.372668250122565 35.0 27.0 35.0 2.0 40.0 40-41 28.02688265449405 35.0 26.0 35.0 2.0 39.5 42-43 27.684415890408353 35.0 26.0 35.0 2.0 39.0 44-45 27.358176772701093 35.0 25.5 35.0 2.0 39.0 46-47 26.986257014884167 34.5 24.0 35.0 2.0 38.0 48-49 26.601004812246238 34.0 22.5 35.0 2.0 37.0 50-51 26.190559715168334 34.0 19.5 35.0 2.0 37.0 52-53 25.72056485584681 33.0 12.5 35.0 2.0 36.0 54-55 25.278079766194328 33.0 3.5 35.0 2.0 36.0 56-57 24.831931943128865 33.0 2.0 35.0 2.0 35.0 58-59 24.37112366663652 33.0 2.0 35.0 2.0 35.0 60-61 23.95069470557716 33.0 2.0 35.0 2.0 35.0 62-63 23.478926788170728 33.0 2.0 35.0 2.0 35.0 64-65 22.99465583633603 33.0 2.0 35.0 2.0 35.0 66-67 22.50896048817406 32.0 2.0 35.0 2.0 35.0 68-69 22.003643693863076 31.0 2.0 35.0 2.0 35.0 70-71 21.47245572114675 31.0 2.0 35.0 2.0 35.0 72-73 20.928690935746282 30.5 2.0 35.0 2.0 35.0 74-75 20.393645074230445 29.5 2.0 35.0 2.0 35.0 76-77 19.61098986619956 27.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6780099125313654 1101 2 0.587790602496483 1101 3 0.5769413401899683 1101 4 0.5566183217710616 1101 5 0.4710662627589599 1101 6 0.38574580391603774 1101 7 0.3874392754412952 1101 8 0.11548972059731 1101 9 0.19270089292954395 1101 10-11 0.39813386946061513 1101 12-13 0.7618231106692548 1101 14-15 0.5213256613015389 1101 16-17 0.6900103962133812 1101 18-19 0.656573207532773 1101 20-21 0.4309564776533641 1101 22-23 0.3673702826326952 1101 24-25 0.6579164244954541 1101 26-27 0.5208956610175406 1101 28-29 0.483685034690577 1101 30-31 0.41032799582490753 1101 32-33 0.4830060119566255 1101 34-35 0.17779802179644832 1101 36-37 0.08782796558941897 1101 38-39 0.11511826379491907 1101 40-41 -0.10077731667261602 1101 42-43 -0.23692904445477936 1101 44-45 -0.17366476010509757 1101 46-47 0.15814623830999608 1101 48-49 0.3121382562044914 1101 50-51 0.05827146391809279 1101 52-53 0.22366335589701336 1101 54-55 0.2529246147238702 1101 56-57 0.13707649845471082 1101 58-59 0.1479709565254801 1101 60-61 0.1392262076559625 1101 62-63 0.4133681893009218 1101 64-65 0.561667568065694 1101 66-67 0.8901442693797179 1101 68-69 1.1922789561682414 1101 70-71 1.1358164172392158 1101 72-73 1.201064825095557 1101 74-75 1.1251293381742933 1101 76-77 1.2357335202025013 1102 1 0.8341631918239258 1102 2 1.0356412534422006 1102 3 1.0923920893916375 1102 4 1.0748448017514107 1102 5 0.8705749829751213 1102 6 0.6445028660629362 1102 7 0.5061079072270971 1102 8 0.6913924472001156 1102 9 0.9535213301685559 1102 10-11 1.2723416808091699 1102 12-13 1.146974179202779 1102 14-15 1.2445631950770206 1102 16-17 1.4687871095516272 1102 18-19 1.1769490365674073 1102 20-21 1.2948351315467619 1102 22-23 1.4598537019399949 1102 24-25 1.077457077897563 1102 26-27 0.9747890533044483 1102 28-29 0.9854904878961754 1102 30-31 1.0565574234839588 1102 32-33 1.0658112195223346 1102 34-35 1.0214482305903587 1102 36-37 1.2463639518335867 1102 38-39 1.1557225379290408 1102 40-41 1.1756903816815978 1102 42-43 1.1260775878223015 1102 44-45 1.2674430758074564 1102 46-47 1.379688856831244 1102 48-49 1.4137963254749408 1102 50-51 1.1800720043258544 1102 52-53 1.426168882794581 1102 54-55 1.3318978399756496 1102 56-57 1.292308628152572 1102 58-59 1.4164676403869372 1102 60-61 1.2440775955213539 1102 62-63 1.4216830996423582 1102 64-65 1.1700807343639 1102 66-67 1.019006956680137 1102 68-69 0.6187835005062432 1102 70-71 1.0931119220291983 1102 72-73 1.3983996322682444 1102 74-75 1.3713956118171318 1102 76-77 1.075527182693289 1103 1 0.44220056924660867 1103 2 0.3818750316715409 1103 3 0.2530934548683206 1103 4 0.24597582285702657 1103 5 0.17750704781602167 1103 6 0.2753007539614529 1103 7 0.3227076085771756 1103 8 0.5133493738769417 1103 9 0.29511371921395124 1103 10-11 0.5170652756921044 1103 12-13 0.7980194539145273 1103 14-15 0.6436610875938342 1103 16-17 0.27148573483834326 1103 18-19 0.6142440996071699 1103 20-21 0.6060660618258567 1103 22-23 0.6503738762037585 1103 24-25 0.9240025912213525 1103 26-27 0.9473024848535765 1103 28-29 0.9002944411590015 1103 30-31 1.3097632918008983 1103 32-33 1.433774088408864 1103 34-35 1.0954025710204753 1103 36-37 1.1743185077842604 1103 38-39 1.169531248038492 1103 40-41 1.3792803384235448 1103 42-43 1.2856551675264214 1103 44-45 1.403340334078056 1103 46-47 1.4862945619683288 1103 48-49 1.4741767192617914 1103 50-51 1.411006132006392 1103 52-53 1.1807063602803822 1103 54-55 1.3059199154386327 1103 56-57 1.4767518923653853 1103 58-59 1.3895852992178952 1103 60-61 1.0237501582147424 1103 62-63 0.6969759766878774 1103 64-65 0.5909808405847485 1103 66-67 0.5966482381038034 1103 68-69 0.5229230346739477 1103 70-71 0.5501117219029616 1103 72-73 0.9230882820319408 1103 74-75 0.9507187233182286 1103 76-77 0.6722497307620685 1104 1 0.4665301261087862 1104 2 0.4386296566536174 1104 3 0.376483674286078 1104 4 0.07381893199226397 1104 5 0.4871227082204399 1104 6 0.27872826005638984 1104 7 0.5018557514138351 1104 8 0.4948566160969321 1104 9 0.2764918921667672 1104 10-11 0.5489919930304055 1104 12-13 0.6282037361459913 1104 14-15 0.7101891243068721 1104 16-17 0.6495260331317638 1104 18-19 0.4071452899126413 1104 20-21 0.4012654021384918 1104 22-23 -0.14319417198209905 1104 24-25 -0.1742155358401689 1104 26-27 -0.15856658154539005 1104 28-29 -0.2803881918540476 1104 30-31 0.004855074390889058 1104 32-33 -0.04580787057579272 1104 34-35 0.23140214078958365 1104 36-37 0.22036038359094334 1104 38-39 0.2258162042983507 1104 40-41 0.01894045601998684 1104 42-43 0.2185248075665811 1104 44-45 -0.17400800953301498 1104 46-47 -0.4091306495618596 1104 48-49 -0.5261531244209863 1104 50-51 -0.38570260168068415 1104 52-53 -0.5861192527986354 1104 54-55 -0.4222279405774252 1104 56-57 -0.45010885623636554 1104 58-59 -0.3249314080816923 1104 60-61 -0.32492329653930696 1104 62-63 -0.45972486943287194 1104 64-65 -0.38928590256740847 1104 66-67 -0.020496462885731148 1104 68-69 0.0362530217670205 1104 70-71 -0.1452095171620016 1104 72-73 -0.25128071266187746 1104 74-75 -0.11485922017811134 1104 76-77 -0.069224527089645 1105 1 -0.16742977364058476 1105 2 -0.24620743607821538 1105 3 -0.32326569914750536 1105 4 0.0020144207140688764 1105 5 -0.03129834441114099 1105 6 0.006297182362153819 1105 7 -0.005913672145062776 1105 8 0.13608468627236903 1105 9 0.09741921046250468 1105 10-11 0.05431780756674698 1105 12-13 0.4048328088276989 1105 14-15 0.47409889874296596 1105 16-17 0.271769140901192 1105 18-19 0.03947611698030329 1105 20-21 -0.19591504898932754 1105 22-23 0.3020439232559937 1105 24-25 0.37622306065105704 1105 26-27 -0.14183725823711768 1105 28-29 0.05094012894796052 1105 30-31 0.09602048792472218 1105 32-33 -0.1563968430068705 1105 34-35 0.08986078740612413 1105 36-37 0.09968369186162107 1105 38-39 0.20470091607780105 1105 40-41 0.27069484198489846 1105 42-43 0.3383243063134458 1105 44-45 0.3110797097652309 1105 46-47 0.0956312552000469 1105 48-49 -0.13292004171421823 1105 50-51 0.026202160224077176 1105 52-53 0.2527905216374542 1105 54-55 0.032972560675705864 1105 56-57 0.019815955793710316 1105 58-59 -0.22098403966063884 1105 60-61 -0.25374535167714996 1105 62-63 -0.5549003080293637 1105 64-65 -0.507832268482197 1105 66-67 -0.37751400674537905 1105 68-69 -0.533546476979847 1105 70-71 -0.2368761838286666 1105 72-73 -0.32991479787490974 1105 74-75 -0.7418642327094425 1105 76-77 -0.6810039757111994 1106 1 0.44763657052406813 1106 2 0.8519056771287481 1106 3 0.5144020362758397 1106 4 0.8216007931288942 1106 5 0.6500246638576641 1106 6 0.9910658371662464 1106 7 0.43024530384853676 1106 8 0.6188689536740384 1106 9 0.7586483431445856 1106 10-11 0.7882130194118204 1106 12-13 0.9485049567786135 1106 14-15 1.060799440621345 1106 16-17 0.6922186437105822 1106 18-19 0.8125528237097228 1106 20-21 1.2487814803639807 1106 22-23 0.9194321569324551 1106 24-25 1.1396215818918165 1106 26-27 1.1228581461091593 1106 28-29 1.1822971999296534 1106 30-31 1.5222294017304314 1106 32-33 1.552285711259092 1106 34-35 1.405706511441899 1106 36-37 1.3221913325343735 1106 38-39 1.516874742164216 1106 40-41 1.6203907775844968 1106 42-43 1.739170561602542 1106 44-45 1.728807320562801 1106 46-47 1.4501553459756131 1106 48-49 1.2433573100192277 1106 50-51 1.0684055489952549 1106 52-53 1.4145665327375205 1106 54-55 1.276633458556315 1106 56-57 0.877952569147542 1106 58-59 0.483570363804386 1106 60-61 0.49183743321525597 1106 62-63 0.7082794618441959 1106 64-65 0.46944883952472694 1106 66-67 0.512913433426121 1106 68-69 0.3913718037594087 1106 70-71 0.33017111021692 1106 72-73 0.1980072516378968 1106 74-75 0.32559556391428757 1106 76-77 0.39729338864733066 1107 1 0.5103462266438115 1107 2 0.5956845278742193 1107 3 0.47781501682682403 1107 4 0.415370505962656 1107 5 0.4451232059185841 1107 6 0.528062588786014 1107 7 0.48296810677030777 1107 8 0.20737994569753226 1107 9 -0.20793637708792545 1107 10-11 -0.2139948179687181 1107 12-13 -0.05816866649180952 1107 14-15 -0.09397909766649803 1107 16-17 -0.25493769711584235 1107 18-19 -0.3694006849536109 1107 20-21 -0.5579015755895185 1107 22-23 -0.4984946572377922 1107 24-25 -0.5340046362507707 1107 26-27 -0.5195812636407027 1107 28-29 -0.356647990286298 1107 30-31 -0.27769749497579 1107 32-33 -0.2446227268654546 1107 34-35 -0.35730322190794084 1107 36-37 -0.05703789935045123 1107 38-39 0.005363921108109793 1107 40-41 -0.16255139417287268 1107 42-43 -0.17734429782100491 1107 44-45 -0.27968798962508856 1107 46-47 -0.38907341427506026 1107 48-49 -0.188128986060903 1107 50-51 0.031125816883452728 1107 52-53 -0.139724453744261 1107 54-55 -0.5521427097699139 1107 56-57 -0.41079070269648454 1107 58-59 -0.23915002199235502 1107 60-61 -0.40446790273565014 1107 62-63 -0.25957867060246187 1107 64-65 0.16043414222542296 1107 66-67 0.21642651832616622 1107 68-69 0.03195415403030566 1107 70-71 -0.04271169459138946 1107 72-73 -0.1352081731570891 1107 74-75 -0.16830266922626436 1107 76-77 -0.0988126818737669 1108 1 0.02866634901900511 1108 2 0.09135411485794975 1108 3 0.10440937088050362 1108 4 -0.03915320423064372 1108 5 -0.0507410688693426 1108 6 -0.0675875294172954 1108 7 0.2663139557481955 1108 8 0.4013891238368714 1108 9 0.4150367838076363 1108 10-11 0.2561901354452729 1108 12-13 0.34756906122338904 1108 14-15 0.15167519241833105 1108 16-17 0.17601210124322364 1108 18-19 0.11370875740489339 1108 20-21 -0.6930844430627481 1108 22-23 -0.649850518731327 1108 24-25 -0.7200204894005395 1108 26-27 -0.8998204515144295 1108 28-29 -0.8002024333401145 1108 30-31 -0.9537981763831098 1108 32-33 -0.6814270656222341 1108 34-35 -0.6254244845974135 1108 36-37 -0.8734167061923372 1108 38-39 -0.6909175418316771 1108 40-41 -0.8740935997075674 1108 42-43 -0.9625897435170145 1108 44-45 -0.9921813841460185 1108 46-47 -0.7817003090045844 1108 48-49 -0.6810757250401807 1108 50-51 -0.9243713323308427 1108 52-53 -1.0193545469162828 1108 54-55 -0.8924291789675216 1108 56-57 -0.903793066762681 1108 58-59 -1.1601790861002677 1108 60-61 -0.9826322748674805 1108 62-63 -0.822062330733182 1108 64-65 -0.8594407013290208 1108 66-67 -0.8756357817711802 1108 68-69 -1.2497841299357653 1108 70-71 -1.2227172571000828 1108 72-73 -1.0744850470272027 1108 74-75 -0.892057362592972 1108 76-77 -0.7112059512382487 1109 1 -0.4452025904807826 1109 2 -0.32987265613547834 1109 3 -0.3471317776454157 1109 4 -0.3134357248398416 1109 5 -0.3440874430484655 1109 6 -0.5689652016263196 1109 7 -0.4455876790149702 1109 8 -0.5537932151319751 1109 9 -0.5517928934314327 1109 10-11 -0.5306898421990027 1109 12-13 -0.7801129284370845 1109 14-15 -0.37997214585189454 1109 16-17 -0.6389131467657236 1109 18-19 -0.7810907395910505 1109 20-21 -0.8573012936984874 1109 22-23 -0.6829928968227073 1109 24-25 -0.8675990837836594 1109 26-27 -0.7616200882337267 1109 28-29 -0.8791880418352953 1109 30-31 -0.7828215101821847 1109 32-33 -0.4746896057926939 1109 34-35 -0.3194135861762888 1109 36-37 -0.21390014897562537 1109 38-39 -0.15159659730185027 1109 40-41 0.027247744431036836 1109 42-43 -0.12335542746280126 1109 44-45 -0.08180273387355896 1109 46-47 -0.17323866306354319 1109 48-49 0.11947757941699422 1109 50-51 0.17741153758672112 1109 52-53 0.38603039090682856 1109 54-55 0.3258186902514275 1109 56-57 0.2139741541399296 1109 58-59 0.49229261992180895 1109 60-61 0.11743199787249736 1109 62-63 -0.038544096836297115 1109 64-65 -0.16035728649040237 1109 66-67 -0.10750108846392692 1109 68-69 0.03370895375852356 1109 70-71 -0.08308612673819837 1109 72-73 0.1253444904885157 1109 74-75 0.15615278132207422 1109 76-77 0.23911401522814657 1110 1 -0.5915487618560462 1110 2 -0.2562438137039891 1110 3 -0.6349930989110248 1110 4 -0.25669669115136884 1110 5 -0.521971981098794 1110 6 -0.5588742111328671 1110 7 -0.45333018181653273 1110 8 -0.816234033778322 1110 9 -0.4480358493088161 1110 10-11 -0.6083829624169041 1110 12-13 -0.4749060416845978 1110 14-15 -0.3523747345502031 1110 16-17 -0.4162545300142497 1110 18-19 -0.3169963282410997 1110 20-21 -0.3333579791137353 1110 22-23 -0.3760621772096151 1110 24-25 -0.4043598865589537 1110 26-27 -0.5715373549801086 1110 28-29 -0.13566917082517804 1110 30-31 -0.029227734210753198 1110 32-33 0.14269456497131117 1110 34-35 0.17539346786573873 1110 36-37 0.0871834797059492 1110 38-39 -0.08650112966862267 1110 40-41 -0.1717034788736811 1110 42-43 0.030797588842567336 1110 44-45 0.17946116000482704 1110 46-47 -0.11066326213165922 1110 48-49 -0.26606045482374086 1110 50-51 -0.3669845311092246 1110 52-53 -0.31789126182798455 1110 54-55 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-0.38991015817501307 2314 5 -0.5558801477567101 2314 6 -0.5844092348436476 2314 7 -0.45138782263757804 2314 8 -0.7672200872894379 2314 9 -0.6207133301914496 2314 10-11 -0.5613201244726511 2314 12-13 -0.43483175957004505 2314 14-15 -0.517442454640495 2314 16-17 -0.7779117473659909 2314 18-19 -0.9731790674925058 2314 20-21 -1.0410438593874147 2314 22-23 -0.6722741393423597 2314 24-25 -0.6524897096467797 2314 26-27 -0.701222640296919 2314 28-29 -0.7757268291449471 2314 30-31 -0.9006426816270654 2314 32-33 -1.1789168057043113 2314 34-35 -0.9890792953589269 2314 36-37 -1.490053733096122 2314 38-39 -1.0028965659994853 2314 40-41 -0.9253475896756491 2314 42-43 -0.6834743764399498 2314 44-45 -0.6825963408263647 2314 46-47 -0.7476761411979247 2314 48-49 -0.6389234222586033 2314 50-51 -0.49166955394506573 2314 52-53 -0.594064989232745 2314 54-55 -1.0732887320910969 2314 56-57 -0.8462736869177192 2314 58-59 -1.1390276953113947 2314 60-61 -0.9862962055560338 2314 62-63 -1.039294432876364 2314 64-65 -1.0581251518534174 2314 66-67 -0.8807269810415832 2314 68-69 -0.9304544445732468 2314 70-71 -0.7047464408666109 2314 72-73 -0.6259237155178532 2314 74-75 -0.38381066856652524 2314 76-77 -0.2653281582605942 2315 1 -0.7114926334929166 2315 2 -0.649817813372799 2315 3 -0.8650932409750496 2315 4 -0.5203387595338498 2315 5 -0.6210158814970939 2315 6 -0.7290547108844763 2315 7 -1.0888575182468045 2315 8 -1.08111371513656 2315 9 -0.7527806134215282 2315 10-11 -1.0883022561059477 2315 12-13 -1.2076273957377737 2315 14-15 -1.1820177442157842 2315 16-17 -0.9869858546959129 2315 18-19 -0.713470438597831 2315 20-21 -0.3846470140370819 2315 22-23 -0.6703024833009401 2315 24-25 -0.8799409981445763 2315 26-27 -0.8985940149938756 2315 28-29 -1.1332026604579895 2315 30-31 -1.090984185922057 2315 32-33 -1.20936710650345 2315 34-35 -1.209519419868819 2315 36-37 -1.3695806852974926 2315 38-39 -1.38687938602882 2315 40-41 -1.4839959390216713 2315 42-43 -1.2842795005008867 2315 44-45 -1.296503437291598 2315 46-47 -1.3136012856114405 2315 48-49 -1.0232878531346614 2315 50-51 -1.1838330629690432 2315 52-53 -1.441428133107518 2315 54-55 -1.4672645185087418 2315 56-57 -1.3224417154113297 2315 58-59 -1.1759728630705162 2315 60-61 -1.1331228494757006 2315 62-63 -1.10588926691306 2315 64-65 -1.2000357501593655 2315 66-67 -1.0437234492706082 2315 68-69 -1.047855899184004 2315 70-71 -1.0622334960280888 2315 72-73 -0.9294203184330598 2315 74-75 -0.9748622683390558 2315 76-77 -1.0567067461508088 2316 1 -1.8096667830017665 2316 2 -1.262413248350164 2316 3 -1.303122939785034 2316 4 -2.339295733521624 2316 5 -1.9917436054075708 2316 6 -2.1061090899922377 2316 7 -1.9287289238266325 2316 8 -2.027682020021082 2316 9 -2.759404573844254 2316 10-11 -2.5301850306375933 2316 12-13 -3.164876886354822 2316 14-15 -3.900142659979302 2316 16-17 -3.075436141298148 2316 18-19 -3.051559994859872 2316 20-21 -2.839189045067819 2316 22-23 -2.745804136317993 2316 24-25 -2.7112440671273177 2316 26-27 -2.7541869208947887 2316 28-29 -3.2587843876824287 2316 30-31 -2.9603859285270886 2316 32-33 -3.033203850258058 2316 34-35 -3.1097655885954794 2316 36-37 -2.8111483162901756 2316 38-39 -2.9575270357319106 2316 40-41 -2.7066458403163374 2316 42-43 -3.1708516108922993 2316 44-45 -3.1829942842547254 2316 46-47 -3.118489776331554 2316 48-49 -2.984998904168094 2316 50-51 -3.120334612920285 2316 52-53 -3.068819458137444 2316 54-55 -2.9687669009146127 2316 56-57 -2.920712984627155 2316 58-59 -3.0563887732605544 2316 60-61 -2.81932511216667 2316 62-63 -2.6431059350332156 2316 64-65 -2.4531138502600527 2316 66-67 -2.5690915131397 2316 68-69 -2.865665275001593 2316 70-71 -2.7122715510969435 2316 72-73 -2.6427348356490157 2316 74-75 -2.747489178029621 2316 76-77 -2.8915199988285423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10761.0 3 1930.0 4 3476.0 5 4398.0 6 4139.0 7 4032.0 8 5160.0 9 5748.0 10 8402.0 11 10232.0 12 6581.0 13 4069.0 14 3624.0 15 3513.0 16 3697.0 17 3913.0 18 4246.0 19 4338.0 20 4391.0 21 4686.0 22 5245.0 23 5693.0 24 6397.0 25 6996.0 26 8160.0 27 9020.0 28 10100.0 29 11387.0 30 12728.0 31 14671.0 32 17861.0 33 23891.0 34 51117.0 35 56818.0 36 25511.0 37 26076.0 38 21184.0 39 5939.0 40 48.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 12.815026549220185 33.12809552483203 43.10588985703168 10.95098806891611 2 14.104517044237424 32.767254880492786 43.098523832418564 10.029704242851228 3 11.901933948229694 34.20529604284439 42.76108301100863 11.131686997917287 4 12.220690837159811 34.46497135199084 43.47237198850747 9.841965822341876 5 11.527338422319177 34.38707235738106 43.32659721081683 10.758992009482938 6 10.981862325049986 36.58288108159574 43.34095020470342 9.094306388650862 7 12.564656147124422 35.94370005736634 41.51537584472527 9.976267950783967 8 10.774365090133516 36.60946586399872 41.86465571210596 10.751513333761805 9 10.879656078610603 36.854433272394886 41.9273213741621 10.338589274832419 10-11 10.59933228991234 36.96757261793533 41.93826359670787 10.494831495444455 12-13 11.431740791038186 37.240162011552194 41.07704594334356 10.25105125406606 14-15 11.963407392068587 37.22942022828132 40.79668776680126 10.010484612848828 16-17 10.671358664089798 38.221055437607696 40.806691927606465 10.30089397069604 18-19 10.719267260462436 38.57998390943583 40.097995344209544 10.602753485892192 20-21 10.952220005998086 38.67147148290783 39.13513307658985 11.24117543450423 22-23 10.67036885401988 39.21311459801196 38.2980014853182 11.818515062649961 24-25 11.031628612271279 39.61247719176297 37.19221526452794 12.163678931437815 26-27 11.410695541025282 40.16040693232156 36.26698625865529 12.161911267997876 28-29 10.669670430892596 40.61722356503019 35.80977270644631 12.903333297630907 30-31 10.563984533892507 40.53165042027207 35.589065190666545 13.315299855168877 32-33 11.439826247359495 40.36726071821725 34.637707893249235 13.55520514117402 34-35 11.083566792262413 39.64513449376504 35.57283323534802 13.698465478624525 36-37 11.791916732418638 39.688603428147076 35.28756520155805 13.23191463787623 38-39 12.424795318296562 39.55358619959129 35.470306722033975 12.551311760078171 40-41 12.073635753424005 39.784038922027406 36.14581002661132 11.996515297937266 42-43 12.0197136967585 39.708150644824975 35.80432930109447 12.46780635732206 44-45 12.274725719413757 39.752296482495794 35.56923487082806 12.403742927262384 46-47 11.461430247864245 39.86072662094837 35.64158349380756 13.036259637379825 48-49 11.635883685198344 39.32021560583502 35.551397998421876 13.492502710544763 50-51 11.4199297956599 39.38025446395094 36.03846597922437 13.161349761164786 52-53 10.896992566228416 39.27114518173756 37.02172994399376 12.81013230804026 54-55 10.657742661561114 39.03105587104868 37.232050715900264 13.079150751489946 56-57 10.231486991502226 39.67710837637762 36.73097522077551 13.360429411344649 58-59 10.22533464569272 39.23772073166813 35.95178388440129 14.58516073823786 60-61 10.723896647063933 38.63487858089073 35.896209067245586 14.745015704799751 62-63 10.949549774738584 38.38109351473275 36.06015699857761 14.609199711951055 64-65 11.365216149304894 37.54665777947058 36.71276899638164 14.375357074842887 66-67 11.066981038880353 37.961515985017726 36.50280352252399 14.468699453577926 68-69 10.676786060756836 38.3179205406956 36.263648624037764 14.741644774509794 70-71 10.76896883453347 38.08673372194917 36.17020516630472 14.974092277212634 72-73 11.65458836529737 37.39946987251696 35.62273308581349 15.323208676372174 74-75 12.15395132036194 36.64120501039665 35.51972504269412 15.68511862654729 76-77 12.181723128149127 36.72642092514502 36.27650744238833 14.815348504317527 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 121657.0 1 67884.0 2 11769.5 3 8301.5 4 7175.0 5 6642.0 6 5416.0 7 4648.5 8 4098.5 9 3623.0 10 3441.5 11 3056.5 12 2832.5 13 2812.0 14 2742.5 15 2691.5 16 2723.0 17 2736.0 18 2909.5 19 3196.0 20 3415.0 21 3812.0 22 4103.0 23 4248.5 24 4563.0 25 4891.5 26 5051.0 27 5346.0 28 5836.0 29 6147.0 30 6263.0 31 6503.0 32 7160.0 33 7850.0 34 8148.0 35 8173.0 36 8554.5 37 9106.5 38 8858.0 39 8439.0 40 8211.5 41 7640.0 42 7081.0 43 6866.0 44 6917.5 45 6969.5 46 7545.0 47 10779.5 48 13439.0 49 12312.0 50 12443.0 51 9903.5 52 6106.0 53 5563.0 54 4695.0 55 4287.5 56 4045.5 57 3886.0 58 4334.0 59 4404.0 60 3901.0 61 3776.0 62 3548.0 63 2451.5 64 1206.0 65 829.0 66 685.5 67 511.5 68 468.0 69 422.5 70 390.0 71 353.5 72 318.0 73 328.5 74 338.0 75 333.5 76 268.5 77 167.5 78 127.0 79 116.5 80 103.5 81 82.0 82 49.0 83 35.0 84 24.5 85 12.5 86 9.0 87 7.0 88 7.5 89 9.0 90 8.0 91 6.0 92 5.0 93 3.0 94 3.0 95 3.0 96 2.0 97 3.5 98 5.5 99 10.5 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003569915607195046 2 0.00856779745726811 3 0.012613701812089163 4 0.01880155553122724 5 0.01308969055971517 6 0.013803673681154179 7 0.016897600540723215 8 0.01880155553122724 9 0.01951553865266625 10-11 0.020348518961011763 12-13 0.02011052458719876 14-15 0.009162783391800617 16-17 0.007139831214390092 18-19 0.013327684933528173 20-21 0.010471752447772134 22-23 0.0157076286716582 24-25 0.023680440194393807 26-27 0.01404166805496718 28-29 0.009162783391800617 30-31 0.007972811522735603 32-33 0.009638772139426623 34-35 0.011185735569211144 36-37 0.009162783391800617 38-39 0.017730580849068728 40-41 0.013327684933528173 42-43 0.015231639924032194 44-45 0.01880155553122724 46-47 0.016421611793097212 48-49 0.014160665241873684 50-51 0.02820233329684086 52-53 0.03224823765166192 54-55 0.026774367053962845 56-57 0.01618361741928421 58-59 0.016540608980003715 60-61 0.019872530213385756 62-63 0.02629837830633684 64-65 0.0233234486336743 66-67 0.01939654146575975 68-69 0.03010628828734489 70-71 0.053786728481738696 72-73 0.06640043029382786 74-75 0.07877613773210401 76-77 0.07603920243325447 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 420178.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.90810751353417 #Duplication Level Percentage of deduplicated Percentage of total 1 86.06094877511381 32.62407698883771 2 6.91864991879411 5.245458499403031 3 2.49959173996118 2.842643772551711 4 1.2742703664538444 1.9322071221137167 5 0.7746893997869158 1.4683504528358826 6 0.43743618605284523 0.9949426782720957 7 0.32235974730771605 0.8554033571283626 8 0.23408763526337634 0.7099055396122433 9 0.1806473326384061 0.6163198656920881 >10 1.136177353446969 7.927260146967048 >50 0.08213829734899201 2.080717148081262 >100 0.06771706957016135 5.06179488068752 >500 0.006270099034274199 1.6255454164642162 >1k 0.0037620594205645193 2.0747749583734674 >5k 0.0 0.0 >10k+ 0.00125401980685484 33.94059917297964 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76202 18.135647273298456 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66593 15.848759335329312 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 1735 0.4129202385655603 No Hit CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA 1727 0.4110162835750563 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1519 0.36151345382195166 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1510 0.3593715044576346 No Hit AGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGC 1183 0.2815473442207826 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1055 0.2510840643727182 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 846 0.2013432402458006 No Hit TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 812 0.19325143153615848 No Hit AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG 786 0.1870635778170204 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 743 0.17682981974306128 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 696 0.16564408417385013 No Hit TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT 642 0.15279238798794798 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 623 0.14827049488550093 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 574 0.13660877056866375 No Hit ACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACC 573 0.13637077619485075 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 544 0.12946893935427367 No Hit GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 494 0.11756922066362352 No Hit TGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTC 492 0.1170932319159975 No Hit AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG 474 0.11280933318736344 No Hit TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 459 0.10923941758016839 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 437 0.10400354135628234 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 435 0.10352755260865634 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0019039549905040245 0.0 2 0.0 0.0 0.0 0.007853814335829101 0.0 3 0.0 0.0 0.0 0.009995763700146128 0.0 4 0.0 0.0 0.0 0.01784957803597523 0.0 5 0.0 0.0 0.0 0.031653251717129405 0.0 6 0.0 0.0 0.0 0.05878461033181175 0.0 7 0.0 0.0 0.0 0.09424577202994922 0.0 8 0.0 0.0 0.0 0.12209111376607057 0.0 9 0.0 0.0 0.0 0.15802826421183402 0.0 10 0.0 0.0 0.0 0.19658335276954053 0.0 11 0.0 0.0 0.0 0.234424458205808 0.0 12 0.0 0.0 0.0 0.2777394342397746 0.0 13 0.0 0.0 0.0 0.3274802583666922 0.0 14 0.0 0.0 0.0 0.391024756174764 0.0 15 0.0 0.0 0.0 0.4598051302067219 0.0 16 0.0 0.0 0.0 0.5552408741057362 0.0 17 0.0 0.0 0.0 0.6278291581187021 0.0 18 0.0 0.0 0.0 0.6889937121886439 0.0 19 0.0 0.0 0.0 0.7430184350441955 0.0 20 0.0 0.0 0.0 0.7989471128902512 0.0 21 0.0 0.0 0.0 0.85749372884825 0.0 22 0.0 0.0 0.0 0.9234181703944518 0.0 23 0.0 0.0 0.0 0.9957684600336048 0.0 24 0.0 0.0 0.0 1.0690707271680098 0.0 25 0.0 0.0 0.0 1.1402310449380977 0.0 26 0.0 0.0 0.0 1.2204351489130798 0.0 27 0.0 0.0 0.0 1.2984973035237446 0.0 28 0.0 0.0 0.0 1.3734655312748407 0.0 29 0.0 0.0 0.0 1.4422459053067986 0.0 30 0.0 0.0 0.0 1.5119782568340085 0.0 31 0.0 0.0 0.0 1.5826625858564705 0.0 32 0.0 0.0 0.0 1.6576308136075664 0.0 33 0.0 0.0 0.0 1.7264111876395243 0.0 34 0.0 0.0 0.0 1.8118511678383924 0.0 35 0.0 0.0 0.0 1.9039549905040245 0.0 36 0.0 0.0 0.0 2.019620256177144 0.0 37 0.0 0.0 0.0 2.1245757750286782 0.0 38 0.0 0.0 0.0 2.2181075639371883 0.0 39 0.0 0.0 0.0 2.3047375160051216 0.0 40 0.0 0.0 0.0 2.394699389306437 0.0 41 0.0 0.0 0.0 2.483947279486313 0.0 42 0.0 0.0 0.0 2.57724107402101 0.0 43 0.0 0.0 0.0 2.6879084578440566 0.0 44 0.0 0.0 0.0 2.804287706638615 0.0 45 0.0 0.0 0.0 2.9289967585166288 0.0 46 0.0 0.0 0.0 3.0913089214570966 0.0 47 0.0 0.0 0.0 3.2276796976519475 0.0 48 0.0 0.0 0.0 3.3766641756588873 0.0 49 0.0 0.0 0.0 3.5092270418727303 0.0 50 0.0 0.0 0.0 3.6417899080865728 0.0 51 0.0 0.0 0.0 3.7812546111409926 0.0 52 0.0 0.0 0.0 3.908105612383323 0.0 53 0.0 0.0 0.0 4.0520922085401905 0.0 54 0.0 0.0 0.0 4.212024427742528 0.0 55 0.0 0.0 0.0 4.345063282703997 0.0 56 0.0 0.0 0.0 4.516419231849359 0.0 57 0.0 0.0 0.0 4.675161479182632 0.0 58 0.0 0.0 0.0 4.863653023242531 0.0 59 0.0 0.0 0.0 5.052858550423868 0.0 60 0.0 0.0 0.0 5.234924246390816 0.0 61 0.0 0.0 0.0 5.42627172293647 0.0 62 0.0 0.0 0.0 5.648796462451628 0.0 63 0.0 0.0 0.0 5.823008344082746 0.0 64 0.0 0.0 0.0 6.024351584328547 0.0 65 0.0 0.0 0.0 6.207845246538372 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGCGT 25 2.226416E-4 56.805904 54 CGTAGGC 45 1.5279402E-6 47.32698 4 ACGTAGG 50 3.1555155E-6 42.594284 3 TACGTAG 50 3.1555155E-6 42.594284 2 ATACGTA 55 6.0840357E-6 38.71286 1 AGTTGGC 60 1.1032276E-5 35.495235 23 TGTTAAG 50 1.5731774E-4 35.495235 18 ATGCTAC 65 1.9083776E-5 32.764835 19 CAGAACG 45 0.004009915 31.566355 46 CTTCGAG 45 0.0040169354 31.555079 55 AGCAGCG 45 0.0040169354 31.555079 59 CTACGTA 45 0.0040192776 31.551321 62 TACGTAT 45 0.0040192776 31.551321 63 GCCTTGA 60 4.5857934E-4 29.579363 3 GAACGAC 50 0.00670801 28.413103 48 CGTATTC 50 0.00671582 28.406334 65 CGACTCG 50 0.00671582 28.406334 51 GCTGAAC 50 0.006723637 28.399569 39 GCCTACG 50 0.006723637 28.399569 60 ACGTATT 50 0.006723637 28.399569 64 >>END_MODULE