FastQCFastQC Report
Thu 26 May 2016
SRR936037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3421576
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36260.10597455675396368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTC12900.015.95938325
TCGAACT14300.015.88719224
CGCGTTC1350.002685043715.77525136
GCCTCGA14100.015.35726821
CCTCGAA14950.014.48453322
GTAGACC22150.014.2603648
CTCGAAC15100.014.10576223
CGCGGGT2555.590251E-613.9205613
ACGCCCG3253.5760422E-713.106359557
CGTGCGC7150.012.9121246
TAAGGCG12300.012.69808117
AGTCGGG7250.012.24675965
GTCGGGT6300.011.839745566
TAGACCA27000.011.5676729
TAAAACG6155.456968E-1211.54776148
CGAATTG2500.001022407911.35967455
GGCCTCG19200.011.27799320
GGCGGAA14050.011.11679520
TGTAGAC29600.010.9108277
ACGCCCC3003.9087507E-410.6467382
Produced by FastQC (version 0.11.5)