FastQCFastQC Report
Thu 26 May 2016
SRR936037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3421576
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48020.1403446832687627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35550.10389948959193074No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11750.039.77084100-101
GGTATCA65550.034.9478951
TACGCAG7900.029.7648100-101
CCTGTCG7450.028.76030396-97
GTATCAA121650.028.32041
TCGTATC5500.027.600086100-101
CCGTACA7250.027.50678100-101
TTATACG8250.027.05356896-97
CGGTATC11000.025.435373100-101
CCGTATA3900.024.422401100-101
CCGTATC7050.023.220766100-101
GCGTATC2950.023.206453100-101
ATACACG7900.022.9784196-97
GCGTACA5450.022.391867100-101
GACCGTA2000.022.32327598-99
CGTACAT19350.022.304373100-101
ATCAACG153700.022.0633013
AGTATCA30550.021.921701100-101
TCAACGC154650.021.8886594
TATCAAC155800.021.8053742
Produced by FastQC (version 0.11.5)