FastQCFastQC Report
Thu 26 May 2016
SRR936036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432467
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36300.10575484046896882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG552.7562186E-432.27142756
GTCAACG2351.4551915E-1121.1450043
TACGCGA900.004862759719.72257857
CGGCGTA1200.001218991917.7523946
CGAACTC11700.015.16878225
CGCGGAA1901.0051387E-414.9485159
TCGAACT12200.014.83913424
GACGCGA5800.014.69634569
GTCGGGT6300.014.65554366
TTAGTCG1707.353108E-414.61620715
GCCTCGA12250.014.48942421
GTAGACC22150.014.4227488
TAAGGCG10850.014.39489517
CTCGAAC12450.013.97173323
CCTCGAA13850.013.58448922
TCGCGGA2102.353602E-413.52405358
CGCGACC6150.013.28382571
TAAGTCG5350.013.2753367
TAGACCA26900.012.7998549
CGACTCG2254.2220025E-412.6206136
Produced by FastQC (version 0.11.5)