##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936036_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3432467 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.713059440921064 40.0 36.0 41.0 30.0 41.0 2 36.58345295089509 39.0 36.0 41.0 30.0 41.0 3 36.445930871294614 39.0 36.0 41.0 30.0 41.0 4 36.34832206689824 39.0 36.0 41.0 29.0 41.0 5 36.26990383301573 39.0 36.0 41.0 29.0 41.0 6 36.19584135841656 39.0 36.0 41.0 29.0 41.0 7 36.05631896825228 39.0 35.0 40.0 29.0 41.0 8 35.93192913435147 39.0 35.0 40.0 28.0 41.0 9 36.103249645226015 39.0 35.0 41.0 29.0 41.0 10-11 36.32616307746003 39.0 36.0 41.0 30.0 41.0 12-13 36.2565491525483 40.0 36.0 41.0 29.5 41.0 14-15 36.1087770108205 39.5 35.0 41.0 28.5 41.0 16-17 35.9737776066019 39.0 35.0 41.0 28.0 41.0 18-19 35.83352731431941 39.0 35.0 41.0 27.5 41.0 20-21 35.66642752865505 39.0 35.0 41.0 27.0 41.0 22-23 35.44899572231867 39.0 35.0 41.0 26.5 41.0 24-25 35.251150411642705 39.0 35.0 41.0 26.0 41.0 26-27 34.31253250213331 38.0 34.0 40.0 24.5 40.5 28-29 34.329317077192584 38.0 33.5 40.0 24.0 40.5 30-31 34.75385225844852 38.0 34.0 40.0 24.0 41.0 32-33 34.64242132553642 38.0 34.0 40.0 24.0 41.0 34-35 34.40310977498109 38.0 34.0 40.0 23.0 41.0 36-37 34.10842405768213 38.0 33.5 40.0 23.0 41.0 38-39 33.81122877510549 37.5 33.0 40.0 22.0 41.0 40-41 33.471799583215216 37.0 33.0 40.0 21.5 41.0 42-43 33.11422673546461 36.5 32.5 39.5 20.5 41.0 44-45 32.760907096848996 36.0 32.0 39.0 19.5 41.0 46-47 32.34693865957051 35.5 32.0 39.0 18.0 40.5 48-49 31.93049969599125 35.0 31.0 38.5 15.5 40.0 50-51 31.48116485897752 35.0 31.0 37.0 11.5 39.5 52-53 30.99226824321982 35.0 31.0 37.0 7.5 39.0 54-55 30.52031119308649 35.0 30.0 36.0 4.5 39.0 56-57 30.07074896859897 34.0 30.0 36.0 2.0 37.5 58-59 29.630391930934806 34.0 29.5 35.5 2.0 37.0 60-61 29.25569408241944 34.0 29.0 35.0 2.0 37.0 62-63 28.877824754032595 34.0 29.0 35.0 2.0 36.0 64-65 28.490094879280704 34.0 29.0 35.0 2.0 36.0 66-67 28.132448323610976 34.0 28.5 35.0 2.0 35.5 68-69 27.767486329803027 34.0 27.5 35.0 2.0 35.0 70-71 27.317837141624373 33.5 26.5 35.0 2.0 35.0 72-73 26.846088250812024 33.0 25.5 35.0 2.0 35.0 74-75 26.280485726446898 33.0 25.0 35.0 2.0 35.0 76-77 24.50822192900908 31.0 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.0066648840667654 1101 2 0.9739828128211983 1101 3 1.1282167064229895 1101 4 0.8062791293861835 1101 5 0.9168372571362795 1101 6 1.0372523538213798 1101 7 1.0469046330824696 1101 8 1.0374573659271036 1101 9 1.0011642999648558 1101 10-11 0.9543245752479166 1101 12-13 1.1004158366495815 1101 14-15 1.049730293799776 1101 16-17 1.0744543588772828 1101 18-19 1.0556602618044124 1101 20-21 1.0008910281122283 1101 22-23 1.0135343038088749 1101 24-25 1.0760162777885185 1101 26-27 0.9857391544780612 1101 28-29 1.0441895355041524 1101 30-31 1.0029348567636944 1101 32-33 1.146628065605391 1101 34-35 1.0819569918971084 1101 36-37 1.0098855623060032 1101 38-39 0.905778267196915 1101 40-41 0.8981461972893854 1101 42-43 0.9713292612489752 1101 44-45 0.8554253754210208 1101 46-47 0.8927062204083782 1101 48-49 0.8812934080008432 1101 50-51 0.7595617091078246 1101 52-53 0.8820200696242289 1101 54-55 0.9928174577797719 1101 56-57 1.0629864516289587 1101 58-59 1.028308914914657 1101 60-61 0.9344804985690764 1101 62-63 0.9912211315892279 1101 64-65 0.9433738756519041 1101 66-67 1.0629952115950978 1101 68-69 1.006521767641125 1101 70-71 1.0213208151053301 1101 72-73 0.9505512304932928 1101 74-75 1.1108761488674155 1101 76-77 1.199754938967736 1102 1 0.7410093786366616 1102 2 0.7363492308933388 1102 3 0.8031344671264975 1102 4 0.657252938781717 1102 5 0.7000580762850177 1102 6 0.7908471616699941 1102 7 0.7728240329516964 1102 8 0.8414438254648218 1102 9 0.8554401813604215 1102 10-11 0.9313941818442757 1102 12-13 0.9345273925546635 1102 14-15 0.9111825155495552 1102 16-17 0.912908066302677 1102 18-19 0.93448045978338 1102 20-21 1.0368936130250006 1102 22-23 0.9929764661432543 1102 24-25 1.0185383545870579 1102 26-27 0.8622906254054499 1102 28-29 1.0094509667372975 1102 30-31 1.1014588808709433 1102 32-33 1.1640932328495168 1102 34-35 0.9780096025659901 1102 36-37 1.0961628886790535 1102 38-39 1.153522476337244 1102 40-41 1.1395904325152841 1102 42-43 1.0686587467354443 1102 44-45 0.9714876296091433 1102 46-47 0.9899823622719808 1102 48-49 0.9294086290634027 1102 50-51 1.06680198428921 1102 52-53 1.1233763678151796 1102 54-55 1.0093581475782223 1102 56-57 1.0218454853157724 1102 58-59 1.0520085030108 1102 60-61 1.0026000627037135 1102 62-63 1.0529682888247152 1102 64-65 1.0505842377063637 1102 66-67 1.043233555359425 1102 68-69 1.2101515274329948 1102 70-71 1.1762781770291646 1102 72-73 1.080923626152007 1102 74-75 1.0885456168575622 1102 76-77 1.1514322543329065 1103 1 0.5620221042714348 1103 2 0.5412654209386645 1103 3 0.5867853255150095 1103 4 0.5714799002343867 1103 5 0.5113337931067861 1103 6 0.5522295224588589 1103 7 0.5827584971642423 1103 8 0.46375203602550386 1103 9 0.5951254824691006 1103 10-11 0.6606672409814252 1103 12-13 0.7579513831318607 1103 14-15 0.6236770836927263 1103 16-17 0.5745092620539154 1103 18-19 0.5306296376103816 1103 20-21 0.5784446147524775 1103 22-23 0.47584536279045864 1103 24-25 0.6425244471532352 1103 26-27 0.5959367641191378 1103 28-29 0.5016581912996116 1103 30-31 0.5993833051505248 1103 32-33 0.4741998899790545 1103 34-35 0.7636802373321672 1103 36-37 0.7078006854145045 1103 38-39 0.6461112236170905 1103 40-41 0.5961655224476559 1103 42-43 0.4725310518904351 1103 44-45 0.5528915843205766 1103 46-47 0.4882082064215538 1103 48-49 0.5895294734226368 1103 50-51 0.6089869925335982 1103 52-53 0.6537056845360105 1103 54-55 0.5246261545949302 1103 56-57 0.5272076282211344 1103 58-59 0.5106325617318284 1103 60-61 0.43921262046096743 1103 62-63 0.34141610435801084 1103 64-65 0.44231466229093996 1103 66-67 0.4322394019271236 1103 68-69 0.4164683044855231 1103 70-71 0.29149079837980807 1103 72-73 0.38930398230130336 1103 74-75 0.3987106118003645 1103 76-77 0.7199907490612567 1104 1 0.7147221637119472 1104 2 0.7395092006919555 1104 3 0.9680389061179682 1104 4 0.9244459199819062 1104 5 0.9334825759432945 1104 6 0.9272688259275697 1104 7 0.8507505542635911 1104 8 0.7583506958888648 1104 9 0.8058518616084456 1104 10-11 0.8441092204945875 1104 12-13 0.8343499440917483 1104 14-15 0.8853465455765175 1104 16-17 0.8372069026792204 1104 18-19 0.658808889498367 1104 20-21 0.6311509992033777 1104 22-23 0.8072856587936599 1104 24-25 0.8062821103043234 1104 26-27 0.6301487361790024 1104 28-29 0.8165023071351598 1104 30-31 0.9025564097481507 1104 32-33 0.8557456149737988 1104 34-35 0.8977214249977763 1104 36-37 0.8807549956903458 1104 38-39 0.8933460821893107 1104 40-41 0.9126396499653282 1104 42-43 0.9143564954568504 1104 44-45 0.8703110457031045 1104 46-47 0.7758073855919676 1104 48-49 0.7813065680263804 1104 50-51 0.863735945215776 1104 52-53 0.7481611931917023 1104 54-55 0.8508165552706686 1104 56-57 0.8205943741709874 1104 58-59 0.6778181708932145 1104 60-61 0.6604463091796866 1104 62-63 0.652384055147607 1104 64-65 0.6828924930019475 1104 66-67 0.6741011459493969 1104 68-69 0.5223352619933017 1104 70-71 0.5311753352969966 1104 72-73 0.5195657484155412 1104 74-75 0.446136159410468 1104 76-77 0.48738279598955714 1105 1 0.09874766317592787 1105 2 0.15448282123979595 1105 3 0.05679493668466051 1105 4 0.15036908862148124 1105 5 -0.07076084288037521 1105 6 0.040833343511401665 1105 7 0.033425152940061764 1105 8 0.011545959479207113 1105 9 0.08770745648899236 1105 10-11 0.15683584213422108 1105 12-13 0.32369423397359043 1105 14-15 0.23530128968214825 1105 16-17 0.18644589912471332 1105 18-19 0.2008734019216334 1105 20-21 0.07339725319552315 1105 22-23 0.07557799257529041 1105 24-25 0.12650469660801633 1105 26-27 -0.04406271856577604 1105 28-29 0.10525802358061043 1105 30-31 0.26390760670710023 1105 32-33 0.28544999548410743 1105 34-35 0.11090320829989508 1105 36-37 0.24368846616003026 1105 38-39 0.1290870650028353 1105 40-41 0.20146541544510654 1105 42-43 0.2672730886415877 1105 44-45 -0.010983214539152186 1105 46-47 -0.018553510084338143 1105 48-49 -0.019986265208004284 1105 50-51 -0.06458787248800135 1105 52-53 -0.12386312517644527 1105 54-55 -0.18466523265352563 1105 56-57 -0.14968540463381785 1105 58-59 -0.01630430676939909 1105 60-61 0.06114854962692107 1105 62-63 -0.16071659512821412 1105 64-65 -0.17640547297333953 1105 66-67 -0.30217893171932175 1105 68-69 -0.35702351785213793 1105 70-71 -0.3922295683148995 1105 72-73 -0.6937809036579488 1105 74-75 -0.9909011568454424 1105 76-77 -0.9439018835551884 1106 1 0.26831761083433747 1106 2 0.21704055885474816 1106 3 0.1133897655675895 1106 4 0.2334313296244659 1106 5 0.20276954482992693 1106 6 0.10429009738112427 1106 7 -0.16791856699001784 1106 8 0.08981281850356027 1106 9 0.24318581153440277 1106 10-11 0.022516698098989707 1106 12-13 0.3558983863672154 1106 14-15 0.30230579464173246 1106 16-17 0.2327266021552461 1106 18-19 0.34326128644057263 1106 20-21 0.3817275893589951 1106 22-23 0.4495446873951465 1106 24-25 0.24791432284408899 1106 26-27 0.1391491788739856 1106 28-29 0.3993719860816398 1106 30-31 0.5566258534323296 1106 32-33 0.39001754765169494 1106 34-35 0.36849372619737863 1106 36-37 0.4183247309724507 1106 38-39 0.5104960805795926 1106 40-41 0.535150820821876 1106 42-43 0.623621139313741 1106 44-45 0.438453744090026 1106 46-47 0.42759630530346726 1106 48-49 0.36060213199535696 1106 50-51 0.2139158864344708 1106 52-53 0.2297052581164536 1106 54-55 0.1461942568801824 1106 56-57 0.3324205708024124 1106 58-59 0.2727990612413045 1106 60-61 0.07825307250217506 1106 62-63 0.08648359920513826 1106 64-65 0.1442644921517271 1106 66-67 0.07772543723385894 1106 68-69 0.19795450065712217 1106 70-71 0.1258751337170203 1106 72-73 0.10947453918007 1106 74-75 0.2833412327004261 1106 76-77 0.18176322997132033 1107 1 -0.10608320944874094 1107 2 0.009683353059884325 1107 3 -0.039107784990520145 1107 4 -0.15925610050118877 1107 5 -0.19313956695447132 1107 6 -0.30934885170351833 1107 7 -0.18502801553944437 1107 8 -0.21941825064176612 1107 9 -0.394123107815318 1107 10-11 -0.1880267211397566 1107 12-13 -0.17641777607999387 1107 14-15 -0.31637255161222555 1107 16-17 -0.177511581286538 1107 18-19 -0.18320959219425959 1107 20-21 -0.33166570530091377 1107 22-23 -0.32940066567176274 1107 24-25 -0.3543592768738364 1107 26-27 -0.6606326611037119 1107 28-29 -0.5299274434726868 1107 30-31 -0.27050315666548386 1107 32-33 -0.13708679155759285 1107 34-35 -0.31729379409771497 1107 36-37 -0.2022271997664049 1107 38-39 -0.13161939190190708 1107 40-41 -0.21419582764531953 1107 42-43 -0.2457061576257189 1107 44-45 -0.301759882623827 1107 46-47 -0.33913552004685954 1107 48-49 -0.3972678683697808 1107 50-51 -0.39299263337060353 1107 52-53 -0.3813691647429245 1107 54-55 -0.35722446073383196 1107 56-57 -0.3744812562289539 1107 58-59 -0.35107659852631556 1107 60-61 -0.5323200023471735 1107 62-63 -0.361630498294808 1107 64-65 -0.49672118261101517 1107 66-67 -0.4235447518709812 1107 68-69 -0.5334854051630025 1107 70-71 -0.5661053373270164 1107 72-73 -0.6975126207161324 1107 74-75 -0.6667823091049634 1107 76-77 -0.49357541522827475 1108 1 0.34794540294363685 1108 2 0.4518798362867855 1108 3 0.4017775316737442 1108 4 0.44673810031734007 1108 5 0.3086732332535007 1108 6 0.216872996888128 1108 7 0.4453779518746259 1108 8 0.30597355488846034 1108 9 0.3850708583473903 1108 10-11 0.3505072681228185 1108 12-13 0.41983546538765637 1108 14-15 0.3707309910085925 1108 16-17 0.40863646043949586 1108 18-19 0.3859486368956553 1108 20-21 0.42361713947089896 1108 22-23 0.3878026254403295 1108 24-25 0.3676131169663961 1108 26-27 0.34099023493852343 1108 28-29 0.3895753066163934 1108 30-31 0.41208778358377174 1108 32-33 0.4530722188946754 1108 34-35 0.3287422622414269 1108 36-37 0.3792923449182837 1108 38-39 0.5098938069936025 1108 40-41 0.4517679341424454 1108 42-43 0.4687117076488292 1108 44-45 0.3935527834033081 1108 46-47 0.2790278682091838 1108 48-49 0.356872709640669 1108 50-51 0.3238348031727405 1108 52-53 0.4317221390053909 1108 54-55 0.5151887282278551 1108 56-57 0.5644817904219863 1108 58-59 0.517674170604085 1108 60-61 0.4520654766684764 1108 62-63 0.4511603194866467 1108 64-65 0.47550882358156343 1108 66-67 0.5988504721813364 1108 68-69 0.5226815009534214 1108 70-71 0.5390792070191317 1108 72-73 0.521693004104403 1108 74-75 0.6239986529344677 1108 76-77 0.5538704787644271 1109 1 -0.1705966108818302 1109 2 -0.2064350240768178 1109 3 -0.3212979211241631 1109 4 -0.07564598108353238 1109 5 -0.2282787455129096 1109 6 -0.30684503940621966 1109 7 -0.22511511278410978 1109 8 -0.22163437385541584 1109 9 -0.2086549588744262 1109 10-11 -0.23162187405400658 1109 12-13 -0.14836708976641688 1109 14-15 -0.09402774882392606 1109 16-17 -0.022722929778673517 1109 18-19 -0.11917621459016203 1109 20-21 -0.04103660599205483 1109 22-23 -0.02354005545236504 1109 24-25 -0.13905535170234629 1109 26-27 -0.3259372241944689 1109 28-29 -0.14199551688059842 1109 30-31 -0.032019955248017595 1109 32-33 -0.04128040614411077 1109 34-35 -0.08844509162134528 1109 36-37 -0.06137032992202762 1109 38-39 0.053324954436384076 1109 40-41 0.019671298189798847 1109 42-43 0.07876927051609073 1109 44-45 0.05608822971466765 1109 46-47 0.0041508132290246635 1109 48-49 0.02745632327184211 1109 50-51 -0.024429561958587698 1109 52-53 -0.09876442277626651 1109 54-55 0.1063166183070301 1109 56-57 0.05476044621331155 1109 58-59 0.0592029326955803 1109 60-61 0.11804190635065126 1109 62-63 0.005736662104698809 1109 64-65 0.1740834954302919 1109 66-67 0.14529124058505616 1109 68-69 0.061111643747395306 1109 70-71 0.09831631213542735 1109 72-73 -0.04601946850851135 1109 74-75 -0.07157610983049167 1109 76-77 0.07998343132362251 1110 1 -0.26804006737047104 1110 2 -0.3017945879931787 1110 3 -0.5241140306898586 1110 4 -0.39388964218434097 1110 5 -0.4208479347530343 1110 6 -0.35907277021452444 1110 7 -0.4023208105678222 1110 8 -0.742997024374823 1110 9 -0.6714940160430984 1110 10-11 -0.45398022724279485 1110 12-13 -0.4298641793483782 1110 14-15 -0.3185301350420602 1110 16-17 -0.37172405973827694 1110 18-19 -0.4847953524792885 1110 20-21 -0.6471094856153314 1110 22-23 -0.5542173106403183 1110 24-25 -0.4934282241198602 1110 26-27 -0.5778098927124589 1110 28-29 -0.3440452414381028 1110 30-31 -0.4099444482904957 1110 32-33 -0.39759982600844523 1110 34-35 -0.4325259878606431 1110 36-37 -0.5108186926683587 1110 38-39 -0.5237628775535867 1110 40-41 -0.47882958511079465 1110 42-43 -0.45843383794181847 1110 44-45 -0.4109239179449986 1110 46-47 -0.5285023667718818 1110 48-49 -0.5265453332631971 1110 50-51 -0.4979662959398077 1110 52-53 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-0.32795948463437696 2314 10-11 -0.4034528817782146 2314 12-13 -0.3556169477553013 2314 14-15 -0.17784552650671515 2314 16-17 -0.3826722783013352 2314 18-19 -0.321740469256099 2314 20-21 -0.30613769013256587 2314 22-23 -0.21592258242284146 2314 24-25 -0.147389481053942 2314 26-27 -0.14673810433621526 2314 28-29 -0.32493414147446487 2314 30-31 -0.43917878364291596 2314 32-33 -0.46479780942934923 2314 34-35 -0.5288993643347268 2314 36-37 -0.9568007926027278 2314 38-39 -0.5723507705545003 2314 40-41 -0.4026265978649022 2314 42-43 -0.43749803488434225 2314 44-45 -0.38780829489145674 2314 46-47 -0.28264107764945123 2314 48-49 -0.16190068008219427 2314 50-51 -0.09018946569780795 2314 52-53 -0.057731535663865685 2314 54-55 -0.1590602370879246 2314 56-57 -0.2774887756879352 2314 58-59 -0.2318596506228161 2314 60-61 -0.18381766498007224 2314 62-63 -0.16212392289529376 2314 64-65 -0.03015770435167653 2314 66-67 -0.04105572334317387 2314 68-69 -0.02552836394090363 2314 70-71 -0.10915810296562611 2314 72-73 -0.15650469361011332 2314 74-75 0.05309187059342335 2314 76-77 -0.017119672885851145 2315 1 -1.0909168216295413 2315 2 -0.8623243354469636 2315 3 -0.7393951104790375 2315 4 -0.8307790845532352 2315 5 -0.8761078816692702 2315 6 -0.8702488732959779 2315 7 -1.2464910308277055 2315 8 -1.3238734515775263 2315 9 -0.9498005349473786 2315 10-11 -1.036116450412642 2315 12-13 -1.0004345572662103 2315 14-15 -0.927696937982212 2315 16-17 -0.836256264307842 2315 18-19 -0.9012722845228396 2315 20-21 -0.8233194893471278 2315 22-23 -0.9488745513747645 2315 24-25 -1.0812510350435431 2315 26-27 -0.9120977694038288 2315 28-29 -0.8197231496191222 2315 30-31 -0.9803299135701096 2315 32-33 -1.0095210305821922 2315 34-35 -1.1066298324501744 2315 36-37 -1.0679455863456937 2315 38-39 -0.9516339502565359 2315 40-41 -1.1195837435486382 2315 42-43 -1.1546137776460412 2315 44-45 -1.1550622711742626 2315 46-47 -1.0086353696944244 2315 48-49 -0.9756928901940682 2315 50-51 -0.9242719007634683 2315 52-53 -0.9205700846083893 2315 54-55 -1.0681785297210915 2315 56-57 -1.199345839686476 2315 58-59 -1.0723639200407575 2315 60-61 -1.196465781947726 2315 62-63 -1.1275145374897662 2315 64-65 -0.9006833997729196 2315 66-67 -0.8689578766449593 2315 68-69 -0.9101695842453346 2315 70-71 -0.9966898704992673 2315 72-73 -0.9696134350037191 2315 74-75 -0.8073552114898028 2315 76-77 -0.8739180598149865 2316 1 -1.8414397500741302 2316 2 -1.5028985148428475 2316 3 -1.6378304870554672 2316 4 -2.6111211086345563 2316 5 -2.025145885854961 2316 6 -1.9955638477922477 2316 7 -1.6590395298220528 2316 8 -1.4346827534185067 2316 9 -1.6669207365763512 2316 10-11 -1.6554553323933234 2316 12-13 -2.1952811073540843 2316 14-15 -3.1776427199003834 2316 16-17 -2.4316721608795646 2316 18-19 -1.9392147493702723 2316 20-21 -1.680703850530719 2316 22-23 -1.9634757073099323 2316 24-25 -1.922371599636385 2316 26-27 -1.86238835284135 2316 28-29 -2.3446876742893394 2316 30-31 -2.725441084703327 2316 32-33 -2.7345291845287036 2316 34-35 -2.694821912816373 2316 36-37 -2.6390024170878803 2316 38-39 -2.6757894080779856 2316 40-41 -2.3847154014724623 2316 42-43 -2.5075081596901434 2316 44-45 -2.506056401465269 2316 46-47 -2.5596061706384674 2316 48-49 -2.2713103279749234 2316 50-51 -2.422885588295177 2316 52-53 -2.3202998294125123 2316 54-55 -2.542532952331083 2316 56-57 -2.401079784905548 2316 58-59 -2.4686550634958557 2316 60-61 -2.351176866105515 2316 62-63 -2.3213460911110246 2316 64-65 -2.2960512078788504 2316 66-67 -2.291623214897843 2316 68-69 -2.495803788578705 2316 70-71 -2.488659224117807 2316 72-73 -2.4304914791437504 2316 74-75 -2.60849404601905 2316 76-77 -3.029413182735734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79476.0 3 6942.0 4 8508.0 5 10894.0 6 12409.0 7 13494.0 8 14950.0 9 14668.0 10 16277.0 11 17818.0 12 15628.0 13 14539.0 14 14893.0 15 15008.0 16 16037.0 17 16729.0 18 17947.0 19 19122.0 20 20289.0 21 21917.0 22 24505.0 23 28159.0 24 32599.0 25 37935.0 26 44937.0 27 52835.0 28 62853.0 29 74660.0 30 89250.0 31 107355.0 32 130550.0 33 164096.0 34 217191.0 35 291828.0 36 416023.0 37 575301.0 38 553613.0 39 159928.0 40 1304.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.26244728836245 26.92327190027602 28.72544615153332 22.088834659828212 2 21.96229264934215 27.050445359771036 28.740863284995584 22.24639870589123 3 22.113760779290708 27.305244338359298 28.252551540714517 22.328443341635477 4 22.006775484704168 27.33613187776387 28.407469700890264 22.2496229366417 5 22.048915745079608 27.593502454295553 28.35377171292522 22.00381008769962 6 22.431515381085234 27.134026325949346 28.269990311700987 22.164467981264433 7 22.15253831319937 27.145973333302255 28.407160461162885 22.294327892335495 8 22.110848695799138 27.22869518721252 28.173856538633572 22.486599578354767 9 21.98796734217663 26.987840587535445 28.442517644391746 22.58167442589618 10-11 22.477540351603135 27.24251175070155 28.223043322425262 22.056904575270057 12-13 22.508467997612875 27.0064870806214 28.243467461442346 22.24157746032338 14-15 22.145231834803273 27.32426629240442 28.183881676179347 22.346620196612964 16-17 22.618036204800813 27.08790030568851 28.221325240687456 22.07273824882322 18-19 22.375894124213172 27.02288523844065 28.11693678387569 22.484283853470487 20-21 22.389055348094633 27.23463774642126 28.17022961561059 22.20607728987352 22-23 22.493338008977062 27.31637038149669 27.750610837304933 22.439680772221315 24-25 22.325962316416046 27.35541340729459 27.881609339260223 22.437014937029144 26-27 22.49403442992402 27.47526176902554 27.83410652521696 22.19659727583348 28-29 22.722436145465732 27.363340341520946 27.637175366298234 22.277048146715085 30-31 22.398893066016583 27.313068679612947 27.880710323429724 22.407327930940745 32-33 22.376534037271423 27.544855657727584 27.739283591482817 22.339326713518176 34-35 22.382993564662446 27.368315086061152 27.838556694212276 22.41013465506413 36-37 22.315399014400843 27.46296719044802 27.966445282959494 22.255188512191644 38-39 22.35809870697505 27.3925332362047 27.98190493534875 22.267463121471497 40-41 22.20024944460624 27.4077588898331 27.87423283254534 22.517758833015325 42-43 22.13605577163943 27.502987755991647 27.792257024377147 22.568699447991776 44-45 22.449297444598784 27.22299100567074 27.93198612168613 22.395725428044347 46-47 22.35490373051099 27.17520384101821 27.796958101228107 22.67293432724269 48-49 22.292372646773444 27.28338369284293 27.776872892986287 22.64737076739734 50-51 22.272510764511637 27.495806900157234 27.639139445212567 22.592542890118565 52-53 22.27731262515735 27.282674091752696 27.741433201109565 22.69858008198039 54-55 22.3319524891694 27.449104228891713 27.655101641466295 22.56384164047259 56-57 22.446446949291417 27.396410876231524 27.631512236284955 22.525629938192104 58-59 22.47865907671077 27.418497137449066 27.707840895316423 22.395002890523745 60-61 22.472517129624503 27.43650951373838 27.604644128663487 22.486329227973638 62-63 22.377444066516375 27.378965233467074 27.669866556940605 22.573724143075953 64-65 22.39690200819023 27.380756556781243 27.518469688322778 22.703871746705747 66-67 22.282446845399853 27.45293844422519 27.688384635154563 22.576230075220398 68-69 22.399294998692984 27.247708935174675 27.772015772772296 22.580980293360042 70-71 22.148943150477994 27.312462716553696 27.821696477148826 22.716897655819484 72-73 22.278011587156495 27.06518026967899 27.88752234340015 22.769285799764365 74-75 22.17205645376432 27.094967099153873 27.72766222206154 23.00531422502027 76-77 22.11190831845099 26.97651386262724 28.002750469794176 22.90882734912759 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3269.0 1 2018.0 2 764.0 3 793.5 4 826.0 5 865.0 6 979.0 7 1111.0 8 1249.0 9 1330.0 10 1387.0 11 1582.0 12 1836.0 13 1952.0 14 2104.5 15 2479.5 16 2956.5 17 3211.0 18 3700.5 19 4779.5 20 6187.0 21 8404.5 22 9804.0 23 11929.0 24 17060.5 25 24056.0 26 28045.0 27 32634.0 28 44482.0 29 56017.5 30 60294.0 31 68217.0 32 82660.0 33 95307.0 34 108775.5 35 116117.0 36 121565.5 37 132280.5 38 140875.5 39 144204.0 40 148718.0 41 155860.0 42 160747.5 43 163007.0 44 163153.5 45 164272.5 46 164608.0 47 163865.5 48 163760.0 49 161666.5 50 157731.0 51 149892.5 52 143896.0 53 140254.0 54 130415.5 55 116044.5 56 100356.0 57 92842.0 58 86676.5 59 72630.0 60 58224.5 61 51700.0 62 45469.0 63 33653.5 64 23814.0 65 19559.0 66 16406.5 67 11351.5 68 7927.5 69 5499.5 70 4593.0 71 3861.5 72 2590.0 73 1771.0 74 1492.0 75 1295.5 76 869.0 77 538.0 78 437.0 79 367.0 80 247.0 81 181.5 82 142.0 83 118.0 84 100.5 85 75.5 86 66.0 87 64.0 88 54.0 89 45.5 90 42.0 91 37.0 92 31.0 93 31.5 94 33.5 95 24.5 96 20.0 97 25.0 98 34.5 99 66.0 100 93.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0032920928300257516 2 0.008681802330510389 3 0.012323497938945953 4 0.01989822480449193 5 0.015295121555429376 6 0.014333713914802386 7 0.018091943782707887 8 0.019577755590949597 9 0.018645481515190097 10-11 0.022054108604685783 12-13 0.02106356739919131 14-15 0.008521567723739222 16-17 0.006263716446509172 18-19 0.011871927683499943 20-21 0.010531783699595655 22-23 0.01449394852157355 24-25 0.02215607608172198 26-27 0.014027811483693798 28-29 0.008477867376437995 30-31 0.008273932422365604 32-33 0.01010934700901713 34-35 0.011697126294295037 36-37 0.009949112402245965 38-39 0.017247070401550837 40-41 0.012891602453861901 42-43 0.015834092505477838 44-45 0.018587214385455124 46-47 0.016445897367695014 48-49 0.013896710441790118 50-51 0.027283583498399256 52-53 0.03019693998514771 54-55 0.025360768217145276 56-57 0.015819525723044098 58-59 0.016402197020393788 60-61 0.020189560453166777 62-63 0.02595800629692871 64-65 0.020262394365335487 66-67 0.019956491934226897 68-69 0.028667427829604773 70-71 0.05309592197099054 72-73 0.06419581018550215 74-75 0.07621340569333951 76-77 0.07305241390521745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3432467.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.5067306609079 #Duplication Level Percentage of deduplicated Percentage of total 1 72.64908383032605 39.59864045101302 2 14.002567503442922 15.264683509426902 3 5.477397145452745 8.956650327900556 4 2.713489743178607 5.9161381833029 5 1.504446004290347 4.1001216574866515 6 0.9417010291982495 3.079742661696525 7 0.6141513974685578 2.343276936478721 8 0.4117933188675453 1.7956406015579727 9 0.31042142135945655 1.522805112487449 >10 1.2971323833030213 11.870003149269861 >50 0.053877615454866655 2.0272454556768476 >100 0.021259456116035285 2.090756907270236 >500 0.001875406083063439 0.7228573681216146 >1k 8.037454586269805E-4 0.7114376783107781 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3630 0.10575484046896882 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.826712973496905E-5 0.0 2 0.0 0.0 0.0 1.7480138920490714E-4 0.0 3 0.0 0.0 0.0 3.4960277840981427E-4 0.0 4 0.0 0.0 0.0 0.0010196747703619584 0.0 5 2.9133564867484524E-5 0.0 0.0 0.0023598187542662463 0.0 6 2.9133564867484524E-5 0.0 0.0 0.00457396968419507 0.0 7 2.9133564867484524E-5 0.0 0.0 0.009963679184679707 0.0 8 2.9133564867484524E-5 0.0 0.0 0.015499056509501766 0.0 9 5.826712973496905E-5 0.0 0.0 0.02138403661273364 0.0 10 5.826712973496905E-5 0.0 0.0 0.028317825051194956 0.0 11 5.826712973496905E-5 0.0 0.0 0.03545554844372867 0.0 12 5.826712973496905E-5 0.0 0.0 0.04699244013125253 0.0 13 5.826712973496905E-5 0.0 0.0 0.06141355474065738 0.0 14 5.826712973496905E-5 0.0 0.0 0.07828188879893092 0.0 15 5.826712973496905E-5 0.0 0.0 0.09852971638183265 2.9133564867484524E-5 16 5.826712973496905E-5 0.0 0.0 0.11694212937808288 2.9133564867484524E-5 17 5.826712973496905E-5 0.0 0.0 0.1351797409851282 2.9133564867484524E-5 18 5.826712973496905E-5 0.0 0.0 0.15461182875174037 2.9133564867484524E-5 19 5.826712973496905E-5 0.0 0.0 0.17686987231049855 5.826712973496905E-5 20 5.826712973496905E-5 0.0 0.0 0.20297354643176468 5.826712973496905E-5 21 5.826712973496905E-5 0.0 0.0 0.229689025415248 5.826712973496905E-5 22 5.826712973496905E-5 0.0 0.0 0.25687064143661104 5.826712973496905E-5 23 5.826712973496905E-5 0.0 0.0 0.28451839449585387 8.740069460245357E-5 24 5.826712973496905E-5 0.0 0.0 0.31292362024165127 1.165342594699381E-4 25 5.826712973496905E-5 0.0 0.0 0.3424359214524131 1.165342594699381E-4 26 5.826712973496905E-5 0.0 0.0 0.3743954421120436 1.165342594699381E-4 27 5.826712973496905E-5 0.0 0.0 0.40565575721485453 1.165342594699381E-4 28 5.826712973496905E-5 0.0 0.0 0.4382562163015697 1.165342594699381E-4 29 5.826712973496905E-5 0.0 0.0 0.47152674738023703 1.165342594699381E-4 30 5.826712973496905E-5 0.0 0.0 0.5095169159674369 1.165342594699381E-4 31 5.826712973496905E-5 0.0 0.0 0.5488763621034084 1.165342594699381E-4 32 5.826712973496905E-5 0.0 0.0 0.5913239661153333 1.165342594699381E-4 33 5.826712973496905E-5 0.0 0.0 0.6356652518436449 1.165342594699381E-4 34 5.826712973496905E-5 0.0 0.0 0.6831820961425121 1.165342594699381E-4 35 5.826712973496905E-5 0.0 0.0 0.7306115397467768 1.165342594699381E-4 36 5.826712973496905E-5 0.0 0.0 0.7820905488676221 1.165342594699381E-4 37 8.740069460245357E-5 0.0 0.0 0.8342396299804193 1.165342594699381E-4 38 8.740069460245357E-5 0.0 0.0 0.886592646047289 1.165342594699381E-4 39 8.740069460245357E-5 0.0 0.0 0.940285806098063 1.165342594699381E-4 40 8.740069460245357E-5 0.0 0.0 0.9978245967113449 1.165342594699381E-4 41 8.740069460245357E-5 0.0 0.0 1.061335768122461 1.456678243374226E-4 42 1.456678243374226E-4 0.0 0.0 1.1306153853773393 1.456678243374226E-4 43 1.456678243374226E-4 0.0 0.0 1.205576047781377 1.456678243374226E-4 44 1.456678243374226E-4 0.0 0.0 1.281876854169319 1.456678243374226E-4 45 1.456678243374226E-4 0.0 0.0 1.3609162156548045 1.456678243374226E-4 46 1.456678243374226E-4 0.0 0.0 1.446860232013884 1.456678243374226E-4 47 1.456678243374226E-4 0.0 0.0 1.531114501610649 1.456678243374226E-4 48 1.456678243374226E-4 0.0 0.0 1.6186899976023077 1.456678243374226E-4 49 1.456678243374226E-4 0.0 0.0 1.711072531797101 1.456678243374226E-4 50 1.7480138920490714E-4 0.0 0.0 1.8090778440113189 1.456678243374226E-4 51 1.7480138920490714E-4 0.0 0.0 1.9083067659499713 1.7480138920490714E-4 52 1.7480138920490714E-4 0.0 0.0 2.017848969851713 1.7480138920490714E-4 53 1.7480138920490714E-4 0.0 0.0 2.1364517124272426 1.7480138920490714E-4 54 1.7480138920490714E-4 0.0 0.0 2.2635031888143424 1.7480138920490714E-4 55 1.7480138920490714E-4 0.0 0.0 2.394662497847758 1.7480138920490714E-4 56 2.0393495407239166E-4 0.0 0.0 2.5341248728684063 1.7480138920490714E-4 57 2.0393495407239166E-4 0.0 0.0 2.6696833501968116 1.7480138920490714E-4 58 2.0393495407239166E-4 0.0 0.0 2.8209448189887913 1.7480138920490714E-4 59 2.0393495407239166E-4 0.0 0.0 2.972031486391566 1.7480138920490714E-4 60 2.0393495407239166E-4 0.0 0.0 3.130751147789622 1.7480138920490714E-4 61 2.0393495407239166E-4 0.0 0.0 3.294627450169222 1.7480138920490714E-4 62 2.0393495407239166E-4 0.0 0.0 3.467243822009068 1.7480138920490714E-4 63 2.0393495407239166E-4 0.0 0.0 3.651309684841835 1.7480138920490714E-4 64 2.0393495407239166E-4 0.0 0.0 3.842746339586076 1.7480138920490714E-4 65 2.0393495407239166E-4 0.0 0.0 4.040242775822754 1.7480138920490714E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACGCG 55 2.7562186E-4 32.271427 56 GTCAACG 235 1.4551915E-11 21.145004 3 TACGCGA 90 0.0048627597 19.722578 57 CGGCGTA 120 0.0012189919 17.75239 46 CGAACTC 1170 0.0 15.168782 25 CGCGGAA 190 1.0051387E-4 14.94851 59 TCGAACT 1220 0.0 14.839134 24 GACGCGA 580 0.0 14.696345 69 GTCGGGT 630 0.0 14.655543 66 TTAGTCG 170 7.353108E-4 14.616207 15 GCCTCGA 1225 0.0 14.489424 21 GTAGACC 2215 0.0 14.422748 8 TAAGGCG 1085 0.0 14.394895 17 CTCGAAC 1245 0.0 13.971733 23 CCTCGAA 1385 0.0 13.584489 22 TCGCGGA 210 2.353602E-4 13.524053 58 CGCGACC 615 0.0 13.283825 71 TAAGTCG 535 0.0 13.27533 67 TAGACCA 2690 0.0 12.799854 9 CGACTCG 225 4.2220025E-4 12.62061 36 >>END_MODULE