FastQCFastQC Report
Thu 26 May 2016
SRR936036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432467
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48780.1421135294235895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35990.10485169995807679No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64800.036.9228551
CGTATCA12050.034.5752100-101
TCGTATA4100.031.211046100-101
CCGTACA6800.030.197008100-101
CGTATAG4300.029.759367100-101
CCTGTCG9300.029.75568496-97
TTATACG6650.029.53195896-97
TACGCAG8100.027.922373100-101
GTATCAA115450.027.580621
CGTATAA5450.025.664042100-101
CGTACAT18600.025.279465100-101
TCGTACA8300.024.739716100-101
ACCGTAC3200.024.177998-99
CGGTATC10100.024.161072100-101
TCGAGTA5600.023.38236100-101
CGTATTA4800.022.939514100-101
GCGTATA5000.022.617119100-101
AGCGTAC4400.022.31806298-99
ATACACG7450.021.96728396-97
TCGCATA2850.021.927956100-101
Produced by FastQC (version 0.11.5)