FastQCFastQC Report
Thu 26 May 2016
SRR936035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3274435
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58780.17951188525654044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC2950.026.4683634
TCGCGCT4050.022.78721237
ATTCGCG3950.022.46926156
CGCGGAA4200.021.97741159
AATTCGC4050.021.91412555
CTCGCGC4350.021.21503336
CGCGCTT4500.020.50880438
TCGCGGA4550.020.28653158
GTAACGT1801.4715079E-719.7184962
CAATTCG5000.019.17282154
CGATCGT5050.018.2746121
CCTCGCG5200.017.7471935
TAGCGCG1200.001221873117.7461055
TAAGGCG8100.016.65295617
CGCTCCG5600.015.84667425
ATCGTTC6000.015.3799583
ACTCGAG5900.015.03976214
AACTCGA6250.014.76566213
CGGCCAA6050.014.67180550
ACGTAAT4850.014.635962532
Produced by FastQC (version 0.11.5)