##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936035_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3274435 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.636710760787736 40.0 36.0 41.0 30.0 41.0 2 36.497193256241154 39.0 36.0 41.0 30.0 41.0 3 36.41687130756909 39.0 36.0 41.0 30.0 41.0 4 36.29798148382851 39.0 36.0 41.0 29.0 41.0 5 36.2246454731885 39.0 36.0 41.0 29.0 41.0 6 36.128201353821346 39.0 36.0 41.0 29.0 41.0 7 35.969503135655465 39.0 35.0 40.0 28.0 41.0 8 35.785831143388094 39.0 35.0 40.0 27.0 41.0 9 35.701408334567645 39.0 35.0 40.0 27.0 41.0 10-11 35.91314623744249 39.0 35.0 40.5 28.5 41.0 12-13 36.09686083247949 39.0 35.0 41.0 29.0 41.0 14-15 36.04815746838767 39.0 35.0 41.0 28.5 41.0 16-17 35.882320308694474 39.0 35.0 41.0 28.0 41.0 18-19 35.73347493537053 39.0 35.0 41.0 27.0 41.0 20-21 35.543240742296 39.0 35.0 41.0 27.0 41.0 22-23 35.29472840352611 39.0 35.0 41.0 26.0 41.0 24-25 35.07481412823892 39.0 34.5 41.0 24.5 41.0 26-27 34.13675275276498 38.0 33.5 40.0 23.5 40.5 28-29 34.16251124239754 38.0 33.5 40.0 23.5 40.5 30-31 34.53171066153398 38.0 34.0 40.0 23.5 41.0 32-33 34.37468601453381 38.0 34.0 40.0 23.0 41.0 34-35 34.10476005784204 38.0 33.5 40.0 22.5 41.0 36-37 33.814685281582925 37.5 33.0 40.0 22.0 41.0 38-39 33.53484097256473 37.0 33.0 40.0 21.0 41.0 40-41 33.15535153392875 36.5 32.5 40.0 19.5 41.0 42-43 32.79701719533294 36.0 32.0 39.0 18.5 41.0 44-45 32.43076698728178 36.0 32.0 39.0 17.5 41.0 46-47 32.04246152389649 35.0 31.5 38.5 14.0 40.0 48-49 31.585735401679983 35.0 31.0 37.5 9.5 40.0 50-51 31.1464938225984 35.0 31.0 37.0 7.5 39.0 52-53 30.69851775955241 35.0 30.5 36.5 4.5 39.0 54-55 30.245577786702132 34.5 30.0 36.0 2.0 38.5 56-57 29.80856972271552 34.0 30.0 35.5 2.0 37.0 58-59 29.385414735671958 34.0 29.0 35.0 2.0 37.0 60-61 29.03921730008383 34.0 29.0 35.0 2.0 36.5 62-63 28.7062306932341 34.0 29.0 35.0 2.0 36.0 64-65 28.3451112940095 34.0 29.0 35.0 2.0 36.0 66-67 27.97909242357842 34.0 28.0 35.0 2.0 35.0 68-69 27.63308738759511 34.0 27.0 35.0 2.0 35.0 70-71 27.221688474500183 33.5 26.5 35.0 2.0 35.0 72-73 26.756000500849765 33.0 25.5 35.0 2.0 35.0 74-75 26.196382429335138 33.0 25.0 35.0 2.0 35.0 76-77 24.50893268609699 31.0 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8086892524925133 1101 2 0.7758589958795099 1101 3 0.8863761404303858 1101 4 0.7891949753796155 1101 5 0.889523011250624 1101 6 0.7354042587632179 1101 7 0.7471711943133599 1101 8 0.9825083512531023 1101 9 0.9271573052464817 1101 10-11 0.8480534998894314 1101 12-13 0.9130054280448263 1101 14-15 0.9233532852385977 1101 16-17 0.8757219321732919 1101 18-19 0.9617127250717132 1101 20-21 0.7681162387956846 1101 22-23 0.8507038973893728 1101 24-25 0.9092719517648291 1101 26-27 0.7260664220696285 1101 28-29 0.7736805897682757 1101 30-31 0.8379275886133897 1101 32-33 0.9813845089189925 1101 34-35 0.8661776625964279 1101 36-37 0.8776632462116538 1101 38-39 1.0060046958712618 1101 40-41 0.9295263469429642 1101 42-43 0.7915035033737823 1101 44-45 0.7653920160784935 1101 46-47 0.7207177743301756 1101 48-49 0.7708467006119655 1101 50-51 0.7271479538643426 1101 52-53 0.6929476155110308 1101 54-55 0.751847458623093 1101 56-57 0.7851047240299742 1101 58-59 0.8663986409036006 1101 60-61 0.86464576427149 1101 62-63 0.7322635473920336 1101 64-65 0.8007881904839529 1101 66-67 0.6065770856754966 1101 68-69 0.7341179352352967 1101 70-71 0.7055610707626165 1101 72-73 0.5800388187909142 1101 74-75 0.6654383218373034 1101 76-77 0.716776599925872 1102 1 0.9952986968394129 1102 2 0.9454002714757621 1102 3 0.9727844767943665 1102 4 1.036078938496459 1102 5 1.0342337151262697 1102 6 1.061108931925709 1102 7 0.8596891183939164 1102 8 1.0356407978494744 1102 9 0.953671221866081 1102 10-11 0.796673527984602 1102 12-13 1.089953007795863 1102 14-15 1.0392379565339027 1102 16-17 1.0208084288166503 1102 18-19 1.0330312449546213 1102 20-21 1.1599247258442489 1102 22-23 1.0907141543213825 1102 24-25 1.0861644114154743 1102 26-27 0.9065380709885815 1102 28-29 0.9860451881029348 1102 30-31 1.2397536945723715 1102 32-33 1.078413168041017 1102 34-35 1.2085729318219407 1102 36-37 1.1075459885331114 1102 38-39 1.2247261920328967 1102 40-41 1.22367499230333 1102 42-43 1.2583864283954043 1102 44-45 1.1407909047424383 1102 46-47 1.074178174040469 1102 48-49 1.0576937700082887 1102 50-51 1.1450533395230345 1102 52-53 1.1237805594629613 1102 54-55 1.0306563522972247 1102 56-57 1.0676952010909986 1102 58-59 1.0359226340517118 1102 60-61 1.0939112188854274 1102 62-63 1.1235529868286491 1102 64-65 1.0829056107667974 1102 66-67 1.1857431001833376 1102 68-69 1.1679655467607724 1102 70-71 1.125616944171039 1102 72-73 1.1024248140595425 1102 74-75 1.0327115890364702 1102 76-77 1.3040877001839384 1103 1 0.6192066353446322 1103 2 0.462005544553044 1103 3 0.6273946362908305 1103 4 0.6832178887050588 1103 5 0.741716915767519 1103 6 0.6880908297969768 1103 7 0.6866302731773004 1103 8 0.8100046829195762 1103 9 0.741066475216094 1103 10-11 0.7267741407129549 1103 12-13 0.6558563281397127 1103 14-15 0.697774539103655 1103 16-17 0.7989229363824322 1103 18-19 0.7411373425234231 1103 20-21 0.8175515246618943 1103 22-23 0.776440213572883 1103 24-25 0.6192838186986975 1103 26-27 0.41522956671158084 1103 28-29 0.46002664529770243 1103 30-31 0.7967967116610808 1103 32-33 0.7460361241228597 1103 34-35 0.8184814238095157 1103 36-37 0.6693842859351804 1103 38-39 0.671139573649576 1103 40-41 0.6645258648061301 1103 42-43 0.6848471609547531 1103 44-45 0.6629312306321111 1103 46-47 0.5076379706781253 1103 48-49 0.4984801001405579 1103 50-51 0.5248144570896507 1103 52-53 0.5896360404973642 1103 54-55 0.6692357575260957 1103 56-57 0.6140696967892438 1103 58-59 0.5616808865332104 1103 60-61 0.5839354675572075 1103 62-63 0.4942946806955888 1103 64-65 0.586043195382647 1103 66-67 0.5897118483777568 1103 68-69 0.5341531630631415 1103 70-71 0.4648936592097108 1103 72-73 0.5137208409091691 1103 74-75 0.5293326297116892 1103 76-77 0.6917522201767135 1104 1 0.3960489458945773 1104 2 0.5383303278197218 1104 3 0.4667874693595664 1104 4 0.4927335262804746 1104 5 0.5895828445735063 1104 6 0.3670325359761222 1104 7 0.620699876974733 1104 8 0.5553567216219193 1104 9 0.6133620020240613 1104 10-11 0.6057901011907632 1104 12-13 0.6755927430485187 1104 14-15 0.5551750334844456 1104 16-17 0.5512926357695846 1104 18-19 0.5444553854787202 1104 20-21 0.5156551246481129 1104 22-23 0.6418880341363362 1104 24-25 0.5501267176289204 1104 26-27 0.48653097599721207 1104 28-29 0.6082665070018294 1104 30-31 0.702547256531254 1104 32-33 0.7021493378542019 1104 34-35 0.8146867133129376 1104 36-37 0.6470416820785587 1104 38-39 0.8384588366546737 1104 40-41 0.6836472301664287 1104 42-43 0.7197938908943868 1104 44-45 0.7555842293837927 1104 46-47 0.6146846417797249 1104 48-49 0.7417874952679604 1104 50-51 0.5306043929597344 1104 52-53 0.5766034392822625 1104 54-55 0.6661801666323832 1104 56-57 0.6352776145679577 1104 58-59 0.5531635076701953 1104 60-61 0.373558374102803 1104 62-63 0.5182903924104529 1104 64-65 0.5450715860565545 1104 66-67 0.43440860725050356 1104 68-69 0.49481285132760533 1104 70-71 0.49874512720085207 1104 72-73 0.46770222521072213 1104 74-75 0.35864519878673917 1104 76-77 0.4778734468347565 1105 1 0.15652316066622518 1105 2 0.2893608575940476 1105 3 0.3624349579878441 1105 4 0.3081881952814385 1105 5 0.4181661958937397 1105 6 0.1925918487963898 1105 7 0.20812918843598993 1105 8 0.3303470886347384 1105 9 0.12673003009961548 1105 10-11 0.21239825045809368 1105 12-13 0.3497875292441748 1105 14-15 0.05964697790890483 1105 16-17 0.1735290876326303 1105 18-19 0.25418190457282464 1105 20-21 0.2634710346656206 1105 22-23 0.32778118392328537 1105 24-25 0.3256494970455108 1105 26-27 0.18599200836565188 1105 28-29 0.26837394878421605 1105 30-31 0.26800488337891437 1105 32-33 0.35907109881222965 1105 34-35 0.35499113169635166 1105 36-37 0.31855697894243207 1105 38-39 0.35055594995619543 1105 40-41 0.42231332125211907 1105 42-43 0.24794091985729239 1105 44-45 0.12710879949674592 1105 46-47 -0.04166505564055001 1105 48-49 0.11484566891754255 1105 50-51 0.2197078456461803 1105 52-53 0.15906660609681822 1105 54-55 0.2091514435427939 1105 56-57 0.2331840986475484 1105 58-59 0.1441510287548482 1105 60-61 -0.1324660401236386 1105 62-63 -0.15337279678781357 1105 64-65 0.06067942355085876 1105 66-67 0.013095802972017623 1105 68-69 -0.2932635090820135 1105 70-71 -0.37298841078349554 1105 72-73 -0.28745106319531644 1105 74-75 -0.21405499547183737 1105 76-77 -0.11216676389183533 1106 1 0.3747904552896699 1106 2 0.3767159999829772 1106 3 -0.05494377249109306 1106 4 0.15302960442493685 1106 5 0.03805071750027622 1106 6 0.19531726595612753 1106 7 0.342545403063788 1106 8 0.43213284735848845 1106 9 0.23889295999983773 1106 10-11 -0.012081368743828591 1106 12-13 0.10179728216488826 1106 14-15 0.12983888594977344 1106 16-17 0.2573267965963666 1106 18-19 0.31071204623372495 1106 20-21 0.10990616212338011 1106 22-23 0.2944500214355017 1106 24-25 0.36336142584178077 1106 26-27 0.3224266328226051 1106 28-29 0.3650586440583865 1106 30-31 0.2949933117132204 1106 32-33 0.2400942024330348 1106 34-35 0.15473232454949937 1106 36-37 0.25138053420501905 1106 38-39 0.2630215804412188 1106 40-41 0.11472240385808874 1106 42-43 0.18909056788910306 1106 44-45 0.05704359470924203 1106 46-47 0.09887130205125771 1106 48-49 0.04186724235698236 1106 50-51 -0.004131003487994889 1106 52-53 0.10210388375061186 1106 54-55 0.23421899125506584 1106 56-57 0.08919122537463053 1106 58-59 0.13543492505196753 1106 60-61 0.06217091243279427 1106 62-63 0.04128390516833136 1106 64-65 0.170979026709702 1106 66-67 0.18928300398437514 1106 68-69 -0.0908907219546009 1106 70-71 -0.24565968917325876 1106 72-73 -0.20685990489037565 1106 74-75 -0.4857738550896116 1106 76-77 -0.4188414256237998 1107 1 0.09286133533677088 1107 2 0.208104098460133 1107 3 0.29719422956927133 1107 4 0.2045578807092454 1107 5 0.11494796860328194 1107 6 -0.06518113608303366 1107 7 0.014470498885785332 1107 8 0.09307965255491979 1107 9 0.1331921827738256 1107 10-11 0.16465348759183485 1107 12-13 0.2672301100669685 1107 14-15 0.27070327661864724 1107 16-17 0.22957525242165389 1107 18-19 0.30624654751463964 1107 20-21 0.1604717000626792 1107 22-23 0.14426283961473274 1107 24-25 0.06908631109380536 1107 26-27 0.011803907500635091 1107 28-29 0.054456442722980114 1107 30-31 0.2613031877636516 1107 32-33 0.38353782513518553 1107 34-35 0.53729019325705 1107 36-37 0.5004376816629161 1107 38-39 0.4603284147551605 1107 40-41 0.47967727814663164 1107 42-43 0.4390004308287061 1107 44-45 0.3689093486405284 1107 46-47 0.28775360148110707 1107 48-49 0.41472880377899557 1107 50-51 0.31477197615578234 1107 52-53 0.5605488354064114 1107 54-55 0.49309640679298994 1107 56-57 0.43466112715254823 1107 58-59 0.509426633157176 1107 60-61 0.37135731607793687 1107 62-63 0.48576616412202256 1107 64-65 0.40881134042976086 1107 66-67 0.4205838908350259 1107 68-69 0.39805833659175605 1107 70-71 0.3429083073558381 1107 72-73 0.2963149311908744 1107 74-75 0.2955202976470339 1107 76-77 0.2981467375498603 1108 1 0.022291332856525514 1108 2 0.137660896912692 1108 3 0.18241238147503225 1108 4 0.13267838464511073 1108 5 0.22046747818893664 1108 6 0.16965360443734312 1108 7 -0.0414016513341835 1108 8 0.17715454142065568 1108 9 0.20311017798846365 1108 10-11 0.060994067461507484 1108 12-13 0.15915931874989298 1108 14-15 0.23400614739763625 1108 16-17 0.2106149417985037 1108 18-19 0.12098935735873084 1108 20-21 0.11102985409156929 1108 22-23 0.10881082510893947 1108 24-25 0.17933644452340758 1108 26-27 -0.10619169849481835 1108 28-29 0.17902676514076887 1108 30-31 0.1919058003912042 1108 32-33 0.24878610204650187 1108 34-35 0.3479180877989876 1108 36-37 0.37431766790658116 1108 38-39 0.21524755225443215 1108 40-41 0.09591097735348342 1108 42-43 0.15143748898835696 1108 44-45 0.11986478290681646 1108 46-47 0.03826239511601415 1108 48-49 0.15846323801055107 1108 50-51 0.2586457348690665 1108 52-53 0.14254770143819329 1108 54-55 0.2801563520318133 1108 56-57 0.29911817015674913 1108 58-59 0.30013621450374117 1108 60-61 0.16465482032053202 1108 62-63 0.1893888941676316 1108 64-65 0.2748049641822412 1108 66-67 0.12204929281892873 1108 68-69 0.23175304279957132 1108 70-71 0.07440273364276706 1108 72-73 0.07920976909163358 1108 74-75 0.05422272100267378 1108 76-77 0.03141554525418755 1109 1 0.20578634330433232 1109 2 0.17955273977685238 1109 3 -0.04498186325767506 1109 4 0.26070606766317894 1109 5 0.09029110582920197 1109 6 0.1301653951163928 1109 7 -0.03454561342201856 1109 8 0.09822146329300097 1109 9 0.24415599832536117 1109 10-11 0.0602399719299882 1109 12-13 0.12071392926869606 1109 14-15 0.19887934697678133 1109 16-17 0.36360798672457406 1109 18-19 0.1918677185774058 1109 20-21 0.19087175926997446 1109 22-23 0.20753605507351125 1109 24-25 0.08931804931871312 1109 26-27 0.0428816773880456 1109 28-29 0.16723466429269251 1109 30-31 0.295674856244581 1109 32-33 0.16233565094234592 1109 34-35 0.198913448795075 1109 36-37 0.2636369766940163 1109 38-39 0.20206688800712413 1109 40-41 0.2975845879811345 1109 42-43 0.2865211450058709 1109 44-45 0.11145167328375294 1109 46-47 0.02548410145755753 1109 48-49 0.22715648228338026 1109 50-51 0.18433499122624752 1109 52-53 0.2288560211499906 1109 54-55 0.3467912160435951 1109 56-57 0.3370595055348673 1109 58-59 0.2595725819018675 1109 60-61 0.25792375409485757 1109 62-63 0.2993999646293055 1109 64-65 0.4419554528416363 1109 66-67 0.3817644318958955 1109 68-69 0.3460285453092986 1109 70-71 0.46113038753836477 1109 72-73 0.35055075598063823 1109 74-75 0.4531749003343464 1109 76-77 0.5869119461284491 1110 1 -0.4083170266129912 1110 2 -0.3701395541014918 1110 3 -0.4373227090868923 1110 4 -0.327119682953267 1110 5 -0.41330743265305614 1110 6 -0.43467046041713786 1110 7 -0.501057103189325 1110 8 -0.3012646571233475 1110 9 -0.3472890117242997 1110 10-11 -0.2684409323323109 1110 12-13 -0.21579086072446074 1110 14-15 -0.47148831176297534 1110 16-17 -0.5155180726340447 1110 18-19 -0.39286197661740374 1110 20-21 -0.4775049614323308 1110 22-23 -0.4838374676953592 1110 24-25 -0.4408706494746397 1110 26-27 -0.3709796027523069 1110 28-29 -0.37955151621441985 1110 30-31 -0.3127337056523558 1110 32-33 -0.1551559398050557 1110 34-35 -0.04740858241068224 1110 36-37 -0.2109004280759521 1110 38-39 -0.1536859386759417 1110 40-41 -0.12263629735603843 1110 42-43 -0.07036960723371521 1110 44-45 -0.14778641734968545 1110 46-47 -0.18759979767285273 1110 48-49 -0.08978082302602175 1110 50-51 -0.12128867486253725 1110 52-53 -0.129150208949266 1110 54-55 -0.27249929859575417 1110 56-57 -0.17481877959923153 1110 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-0.7157976770365408 2314 20-21 -0.6019987332770924 2314 22-23 -0.5278377666625502 2314 24-25 -0.5850195469347952 2314 26-27 -0.526222248061174 2314 28-29 -0.5955341643162768 2314 30-31 -0.775527042617874 2314 32-33 -0.7594197626119197 2314 34-35 -0.7893305677101452 2314 36-37 -0.8781233966234154 2314 38-39 -0.7779043460069275 2314 40-41 -0.5252438026125432 2314 42-43 -0.45036870246460126 2314 44-45 -0.5984784673794863 2314 46-47 -0.49519081056187986 2314 48-49 -0.5100682052339316 2314 50-51 -0.47628368879794536 2314 52-53 -0.5011425892197927 2314 54-55 -0.5475382921777943 2314 56-57 -0.32396701815144624 2314 58-59 -0.47913931413329536 2314 60-61 -0.46080908565292233 2314 62-63 -0.4561881739482061 2314 64-65 -0.41998150277312973 2314 66-67 -0.4437515643809178 2314 68-69 -0.28621382157036734 2314 70-71 -0.26301992882664393 2314 72-73 -0.1979277270042381 2314 74-75 -0.22766466045284872 2314 76-77 -0.128754821825396 2315 1 -1.1885636119137288 2315 2 -1.0301283602996847 2315 3 -1.0819709674535645 2315 4 -0.8765654074892453 2315 5 -0.8232139472527109 2315 6 -0.9291148393380553 2315 7 -1.0662475961776536 2315 8 -1.0438795892689683 2315 9 -0.9271794111092859 2315 10-11 -0.9816670417678068 2315 12-13 -1.1187095587695168 2315 14-15 -1.3326401368137155 2315 16-17 -1.1259319538060524 2315 18-19 -1.005331003346356 2315 20-21 -1.0618273129026932 2315 22-23 -1.0514039630596912 2315 24-25 -1.1160799167416613 2315 26-27 -1.163225214492499 2315 28-29 -1.046267325529513 2315 30-31 -1.2591557098360937 2315 32-33 -1.3612052568651976 2315 34-35 -1.1214257205072329 2315 36-37 -1.3986306167086653 2315 38-39 -1.2749344335706425 2315 40-41 -1.2084112161634408 2315 42-43 -1.3705007376897598 2315 44-45 -1.252261632835733 2315 46-47 -1.2081158705354973 2315 48-49 -1.1213102995013458 2315 50-51 -1.1294082019351315 2315 52-53 -1.203406716418769 2315 54-55 -1.2473917834160382 2315 56-57 -1.3023498493685715 2315 58-59 -1.1675415292228202 2315 60-61 -1.3460590832227162 2315 62-63 -1.3983094626578207 2315 64-65 -1.4129703498215527 2315 66-67 -1.2352879083534916 2315 68-69 -1.1942384898993872 2315 70-71 -1.332231755563516 2315 72-73 -2.7103716450055693 2315 74-75 -3.311480204174675 2315 76-77 -3.02892781329707 2316 1 -2.9695542105682264 2316 2 -2.9141043333603847 2316 3 -2.4009995125202863 2316 4 -2.4045759145677152 2316 5 -3.1415395097695153 2316 6 -2.5540778333742082 2316 7 -2.8311250397481302 2316 8 -2.736561666456872 2316 9 -2.3848670797283305 2316 10-11 -2.332826439922684 2316 12-13 -2.501027716955228 2316 14-15 -2.647417311221382 2316 16-17 -3.8037875850414835 2316 18-19 -3.5495799365808054 2316 20-21 -3.0652203544215553 2316 22-23 -3.132276770292883 2316 24-25 -3.0958090173947497 2316 26-27 -2.8789540306050263 2316 28-29 -2.9684726822146956 2316 30-31 -3.204280703690223 2316 32-33 -3.4014225014874384 2316 34-35 -3.3411807331807992 2316 36-37 -3.2795035808576003 2316 38-39 -3.2490103828847623 2316 40-41 -3.222137421197928 2316 42-43 -3.455748879022728 2316 44-45 -3.140638831094595 2316 46-47 -2.9050937778443213 2316 48-49 -3.0740925203194642 2316 50-51 -3.4083837408622664 2316 52-53 -4.622835187421263 2316 54-55 -3.7450210479937702 2316 56-57 -3.6459817375776034 2316 58-59 -3.6464567601975695 2316 60-61 -3.4192315116612875 2316 62-63 -3.3615237039126455 2316 64-65 -3.2988185892791684 2316 66-67 -3.40291812488676 2316 68-69 -3.2755184934979873 2316 70-71 -3.1349389706542077 2316 72-73 -3.2193757321377454 2316 74-75 -3.214150596369894 2316 76-77 -3.322974848269407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 81656.0 3 7041.0 4 9084.0 5 10427.0 6 12027.0 7 12935.0 8 14714.0 9 15417.0 10 18164.0 11 20015.0 12 16459.0 13 14366.0 14 14464.0 15 15199.0 16 15595.0 17 16391.0 18 17056.0 19 18033.0 20 19629.0 21 20982.0 22 23250.0 23 26899.0 24 30895.0 25 36421.0 26 43083.0 27 50733.0 28 60448.0 29 71436.0 30 84766.0 31 102640.0 32 124792.0 33 157221.0 34 208872.0 35 283969.0 36 404857.0 37 550901.0 38 503096.0 39 139399.0 40 1103.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.83885837014214 26.56690671037095 28.33510378036765 22.259131139119265 2 22.437672114884165 26.79555378536678 28.28756227901487 22.479211820734175 3 22.73498368342796 26.83449950091204 27.784538169538408 22.64597864612159 4 22.43572023967518 26.91394534582433 28.109694431556438 22.540639982944054 5 22.704223442064354 26.824764234217835 28.010282185893026 22.460730137824786 6 22.726790985628135 26.734115124591302 28.09338735318507 22.445706536595498 7 22.663537271427185 26.490408081854618 28.12329141189932 22.72276323481888 8 22.5983770223458 26.798893904716113 27.84875585662058 22.7539732163175 9 22.532120213581898 26.684633154676433 28.05866324075491 22.724583390986762 10-11 22.954421327351234 26.763854372087454 27.78429931504004 22.49742498552127 12-13 23.02186038292973 26.555408618583915 27.683073089952153 22.739657908534205 14-15 22.744220878839958 26.721717987255957 27.870990914607948 22.66307021929614 16-17 22.938484143900748 26.51648678207908 27.96652280582696 22.578506268193212 18-19 22.827490696009363 26.535754081415718 27.732703618720876 22.90405160385404 20-21 22.853057865579036 26.715757550579532 27.704468643009527 22.7267159408319 22-23 22.968520500322946 26.87383992530925 27.3173200707259 22.840319503641908 24-25 22.966675906601818 26.97912920102052 27.19474805140458 22.85944684097308 26-27 22.990738138585794 26.974193863711342 27.210841754133014 22.824226243569846 28-29 23.196730999883634 26.835827058678724 27.16968459915257 22.79775734228507 30-31 22.952603386886032 26.81382511167757 27.249631813736542 22.983939687699863 32-33 22.86700036850041 27.153990257722743 27.178332970127034 22.80067640364982 34-35 22.92017723663204 26.820907251925068 27.29638999633786 22.962525515105035 36-37 22.912745696324627 26.872093442335544 27.490911900874202 22.724248960465623 38-39 22.81727017448155 26.82844136750137 27.550717304887833 22.803571153129248 40-41 22.788766426277096 26.82308405724329 27.393568322882246 22.99458119359737 42-43 22.68111709012272 27.070902830752736 27.14567483798253 23.102305241142016 44-45 22.723410338063736 26.836897884482518 27.49088423389385 22.948807543559894 46-47 22.76551259930228 26.717351118559122 27.422129434284965 23.09500684785363 48-49 22.7964799702352 26.76773176734153 27.354902325848503 23.080885936574767 50-51 22.73403478071457 27.021401624884156 27.15470829790123 23.08985529650004 52-53 22.857286013495585 26.864930650021712 27.267662640619744 23.010120695862955 54-55 22.781261302873006 26.8342882733609 27.319257896125986 23.065192527640107 56-57 22.726069632304327 27.092281077362728 27.1504711640629 23.031178126270042 58-59 22.838158535168024 27.03516550345314 27.211254940393037 22.9154210209858 60-61 22.796159280974 26.889309607723472 27.14928574526798 23.16524536603455 62-63 22.773232371290884 26.82779231931099 27.274887825185534 23.124087484212588 64-65 22.790338635301765 26.873356833487826 27.17282877496624 23.163475756244168 66-67 22.727613618746282 26.940929478440907 27.29127287973661 23.0401840230762 68-69 22.823328087957332 26.72905308411152 27.336958605627217 23.110660222303935 70-71 22.64102889253188 26.632632014777037 27.422176863057253 23.304162229633828 72-73 22.811248687031178 26.496272490489236 27.385645057590292 23.306833764889294 74-75 22.659868755963085 26.51969172521309 27.242727500438228 23.577712018385597 76-77 22.561196195086268 26.406366608166604 27.582124626681924 23.450312570065197 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5316.0 1 3286.5 2 1215.0 3 1211.0 4 1249.0 5 1361.5 6 1537.5 7 1662.5 8 1820.5 9 1917.0 10 1951.0 11 2062.5 12 2294.5 13 2449.0 14 2588.0 15 2971.5 16 3467.5 17 3719.0 18 3994.5 19 4746.5 20 5957.0 21 7767.0 22 8843.0 23 10441.0 24 14046.0 25 19165.5 26 22278.0 27 25273.0 28 33657.5 29 42833.5 30 46620.0 31 53963.5 32 68059.5 33 80776.5 34 92514.0 35 98287.0 36 105104.0 37 117748.5 38 125155.0 39 126734.0 40 130976.5 41 138341.5 42 146054.5 43 150645.0 44 151232.0 45 152076.0 46 153060.0 47 154666.5 48 155546.0 49 155424.0 50 154267.0 51 148044.0 52 142856.0 53 141531.0 54 134607.0 55 121710.0 56 107678.0 57 100944.0 58 95761.5 59 82008.0 60 67745.5 61 62054.0 62 54270.0 63 39826.5 64 28745.0 65 24323.0 66 20968.0 67 15711.5 68 11708.0 69 8073.5 70 6541.0 71 5454.5 72 3727.5 73 2674.5 74 2262.0 75 2015.0 76 1425.5 77 883.5 78 684.0 79 587.5 80 419.0 81 289.5 82 206.5 83 181.0 84 147.5 85 103.0 86 82.0 87 72.0 88 69.5 89 61.5 90 52.0 91 48.0 92 53.0 93 48.5 94 33.5 95 41.0 96 54.0 97 54.5 98 60.5 99 101.5 100 137.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009436742521992343 2 0.014017685493833287 3 0.01413984397308238 4 0.015391968385385571 5 0.012918259180591462 6 0.010078074538050076 7 0.016277617359941488 8 0.017590821011869224 9 0.017163266334497402 10-11 0.020369926414786063 12-13 0.016628822987782624 14-15 0.00849001430781188 16-17 0.006291161681328229 18-19 0.017438122912807857 20-21 0.023790363833760632 22-23 0.020446275464316745 24-25 0.018400120936894457 26-27 0.024278997750757002 28-29 0.00867325202668552 30-31 0.008459474687999608 32-33 0.010826295223450765 34-35 0.012032610206035545 36-37 0.011391278189977814 38-39 0.01461320808017261 40-41 0.011437087619696222 42-43 0.014735366559421703 44-45 0.013345813857963284 46-47 0.023866712883291317 48-49 0.02350023744554404 50-51 0.0352885306930814 52-53 0.03941137936773825 54-55 0.028432386045226122 56-57 0.020812750902064022 58-59 0.016201268310410803 60-61 0.0205531641336597 62-63 0.026309882468273153 64-65 0.025638010832403146 66-67 0.028478195474944533 68-69 0.04255696020840236 70-71 0.054665919463968586 72-73 0.08387706581440768 74-75 0.08378544695497085 76-77 0.08943527662024135 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3274435.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.44295788412444 #Duplication Level Percentage of deduplicated Percentage of total 1 74.6271908187674 39.13670625119138 2 13.092028268037344 13.731693741568984 3 5.020122009665102 7.898101413780993 4 2.3876645871807933 5.008647735477509 5 1.3073481648157579 3.428060237366009 6 0.7969225910467272 2.5075786727502507 7 0.5408776144269597 1.985565536770112 8 0.3872106270199154 1.624517648407267 9 0.26402901513854715 1.2461816268987915 >10 1.4173304281126853 13.55353609101001 >50 0.10375865090314701 3.754427025147094 >100 0.05282171971138017 4.80947310838259 >500 0.0018165360969458941 0.5988451026010491 >1k 8.203711388317268E-4 0.5360319754832669 >5k 5.859793848798049E-5 0.1806338331646301 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5878 0.17951188525654044 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.107923962454592E-5 3.053961981227296E-5 2 0.0 0.0 0.0 3.3593581793500254E-4 6.107923962454592E-5 3 0.0 0.0 0.0 6.107923962454592E-4 6.107923962454592E-5 4 6.107923962454592E-5 0.0 0.0 0.0012521244123031912 6.107923962454592E-5 5 9.161885943681887E-5 0.0 0.0 0.002259931866108199 6.107923962454592E-5 6 9.161885943681887E-5 0.0 0.0 0.0051306561284618565 6.107923962454592E-5 7 9.161885943681887E-5 0.0 0.0 0.009864297199364165 1.2215847924909183E-4 8 9.161885943681887E-5 0.0 0.0 0.015727904203320573 1.2215847924909183E-4 9 1.2215847924909183E-4 0.0 0.0 0.021530431967652433 1.2215847924909183E-4 10 1.526980990613648E-4 0.0 0.0 0.02831022756597703 1.2215847924909183E-4 11 1.526980990613648E-4 0.0 0.0 0.03710563807191164 1.2215847924909183E-4 12 1.526980990613648E-4 0.0 0.0 0.048008282344893086 1.2215847924909183E-4 13 1.526980990613648E-4 0.0 0.0 0.06266729985478411 1.2215847924909183E-4 14 1.526980990613648E-4 3.053961981227296E-5 0.0 0.0820904980553897 1.2215847924909183E-4 15 1.526980990613648E-4 3.053961981227296E-5 0.0 0.10258258294942486 1.526980990613648E-4 16 1.526980990613648E-4 3.053961981227296E-5 0.0 0.12225009810852865 1.526980990613648E-4 17 1.526980990613648E-4 3.053961981227296E-5 0.0 0.14277272262237609 1.526980990613648E-4 18 1.526980990613648E-4 3.053961981227296E-5 0.0 0.16357020371453396 1.526980990613648E-4 19 2.137773386859107E-4 6.107923962454592E-5 0.0 0.18479523948406365 1.526980990613648E-4 20 2.4431695849818367E-4 6.107923962454592E-5 0.0 0.20913531647444522 1.526980990613648E-4 21 2.4431695849818367E-4 6.107923962454592E-5 0.0 0.23536884989318768 1.526980990613648E-4 22 3.053961981227296E-4 6.107923962454592E-5 0.0 0.264045552896912 1.526980990613648E-4 23 3.664754377472755E-4 6.107923962454592E-5 0.0 0.2947378708082463 1.8323771887363775E-4 24 3.664754377472755E-4 6.107923962454592E-5 0.0 0.32628529807432427 2.137773386859107E-4 25 3.664754377472755E-4 6.107923962454592E-5 0.0 0.35780218572058997 2.137773386859107E-4 26 3.664754377472755E-4 6.107923962454592E-5 0.0 0.39264789192639343 3.053961981227296E-4 27 3.664754377472755E-4 6.107923962454592E-5 0.0 0.42489773044815365 3.053961981227296E-4 28 3.664754377472755E-4 9.161885943681887E-5 0.0 0.4574224255482244 3.3593581793500254E-4 29 3.664754377472755E-4 9.161885943681887E-5 0.0 0.49297054300971005 3.3593581793500254E-4 30 4.275546773718214E-4 9.161885943681887E-5 0.0 0.5310534489156145 3.664754377472755E-4 31 4.275546773718214E-4 9.161885943681887E-5 0.0 0.5711825093489411 3.664754377472755E-4 32 4.5809429718409437E-4 9.161885943681887E-5 0.0 0.6150374033993651 3.664754377472755E-4 33 4.5809429718409437E-4 9.161885943681887E-5 0.0 0.6599001049035941 3.9701505755954846E-4 34 4.5809429718409437E-4 9.161885943681887E-5 0.0 0.7077251495296135 3.9701505755954846E-4 35 4.5809429718409437E-4 9.161885943681887E-5 0.0 0.7596730428302898 3.9701505755954846E-4 36 4.5809429718409437E-4 9.161885943681887E-5 0.0 0.814186264195197 4.275546773718214E-4 37 4.5809429718409437E-4 9.161885943681887E-5 0.0 0.8683635497421692 4.275546773718214E-4 38 4.5809429718409437E-4 9.161885943681887E-5 0.0 0.924831306775062 4.275546773718214E-4 39 4.5809429718409437E-4 9.161885943681887E-5 0.0 0.9788864338427851 4.275546773718214E-4 40 4.5809429718409437E-4 9.161885943681887E-5 0.0 1.0384081528569051 4.5809429718409437E-4 41 4.5809429718409437E-4 9.161885943681887E-5 0.0 1.101167071571126 4.5809429718409437E-4 42 5.191735368086402E-4 9.161885943681887E-5 0.0 1.1724770838327834 4.886339169963673E-4 43 5.191735368086402E-4 9.161885943681887E-5 0.0 1.247848865529473 5.191735368086402E-4 44 5.191735368086402E-4 9.161885943681887E-5 0.0 1.3267632431243863 5.191735368086402E-4 45 5.191735368086402E-4 9.161885943681887E-5 0.0 1.409739390154332 5.191735368086402E-4 46 5.191735368086402E-4 9.161885943681887E-5 0.0 1.4988234611467321 5.802527764331862E-4 47 5.497131566209132E-4 9.161885943681887E-5 0.0 1.590992033740172 5.802527764331862E-4 48 5.497131566209132E-4 9.161885943681887E-5 0.0 1.684290572266666 6.413320160577321E-4 49 5.497131566209132E-4 9.161885943681887E-5 0.0 1.7800322803781417 7.32950875494551E-4 50 5.497131566209132E-4 9.161885943681887E-5 0.0 1.8821873086501946 7.32950875494551E-4 51 5.497131566209132E-4 9.161885943681887E-5 0.0 1.9865411895487313 7.32950875494551E-4 52 5.497131566209132E-4 9.161885943681887E-5 0.0 2.095750869997419 7.634904953068239E-4 53 5.497131566209132E-4 9.161885943681887E-5 0.0 2.215985353198338 7.940301151190969E-4 54 5.497131566209132E-4 9.161885943681887E-5 0.0 2.3442822960296965 8.245697349313698E-4 55 5.497131566209132E-4 9.161885943681887E-5 0.0 2.4774044987913943 8.245697349313698E-4 56 6.413320160577321E-4 9.161885943681887E-5 0.0 2.617978368787287 8.245697349313698E-4 57 6.718716358700051E-4 9.161885943681887E-5 0.0 2.762216993160652 9.161885943681887E-4 58 6.718716358700051E-4 9.161885943681887E-5 0.0 2.9171139448485004 9.467282141804616E-4 59 6.718716358700051E-4 9.161885943681887E-5 0.0 3.0734462586675257 9.467282141804616E-4 60 7.02411255682278E-4 9.161885943681887E-5 0.0 3.2274881010006307 9.772678339927347E-4 61 7.32950875494551E-4 9.161885943681887E-5 0.0 3.3969829909587457 0.0010383470736172805 62 7.32950875494551E-4 9.161885943681887E-5 0.0 3.5757924649596036 0.0010383470736172805 63 7.32950875494551E-4 9.161885943681887E-5 0.0 3.7576864405615016 0.0011605055528663723 64 7.32950875494551E-4 9.161885943681887E-5 0.0 3.9470931626372185 0.0011605055528663723 65 7.32950875494551E-4 9.161885943681887E-5 0.0 4.15509851317861 0.0011605055528663723 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 295 0.0 26.468363 4 TCGCGCT 405 0.0 22.787212 37 ATTCGCG 395 0.0 22.469261 56 CGCGGAA 420 0.0 21.977411 59 AATTCGC 405 0.0 21.914125 55 CTCGCGC 435 0.0 21.215033 36 CGCGCTT 450 0.0 20.508804 38 TCGCGGA 455 0.0 20.286531 58 GTAACGT 180 1.4715079E-7 19.718496 2 CAATTCG 500 0.0 19.172821 54 CGATCGT 505 0.0 18.274612 1 CCTCGCG 520 0.0 17.74719 35 TAGCGCG 120 0.0012218731 17.746105 5 TAAGGCG 810 0.0 16.652956 17 CGCTCCG 560 0.0 15.846674 25 ATCGTTC 600 0.0 15.379958 3 ACTCGAG 590 0.0 15.039762 14 AACTCGA 625 0.0 14.765662 13 CGGCCAA 605 0.0 14.671805 50 ACGTAAT 485 0.0 14.6359625 32 >>END_MODULE