FastQCFastQC Report
Thu 26 May 2016
SRR936035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3274435
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67510.20617297335265472No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.1541334611925416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33580.10255204332961258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13050.043.33237100-101
CGGTATC11150.039.23845100-101
GGTATCA82400.035.6010781
CTACGCG1450.034.88836396-97
CGTACAT20050.034.4384100-101
CGAGTAC13500.032.628513100-101
TACGCAG11750.031.915527100-101
GCGTATC4000.030.50656100-101
GCGTACA7150.030.386887100-101
TCGTATC5800.029.762495100-101
CCGTATC6050.029.024582100-101
GTATCAA156850.028.7559781
CCGTACA9150.028.298767100-101
GGGTATC15600.027.473072100-101
CCTGTCG10350.027.3138596-97
CGCTAGT1651.8189894E-1225.24897696-97
TCGAGTA5600.024.979239100-101
TCGTACA10750.024.363718100-101
GGCGTAC3800.024.27955898-99
AGTATCA37400.024.271553100-101
Produced by FastQC (version 0.11.5)