FastQCFastQC Report
Thu 26 May 2016
SRR936034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286492
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59040.17964443546492734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA3100.029.77945559
CGGTTAC2650.026.7865854
TCGCGGA3750.023.66976758
AATTCGC4000.023.07591455
ATTCGCG4100.022.51274556
CGATCGT4500.022.0837141
CCTCGCG5150.021.363635
TCGCGCT4500.021.2966337
CGCGCTT4350.021.21729538
AACTCGA4800.020.70505713
CTCGCGC4650.019.84541736
CGCTCCG5300.019.42175125
CAATTCG4700.018.88401454
TCGCGGG4350.018.77514663
TTACGCG1159.1676693E-418.52219256
ACTCGAG5400.018.40393614
GCGCGAG4300.017.33657624
TTCGCGG5950.017.30478957
CGCGGGG4800.017.01393964
ACGATCG3900.016.3875159
Produced by FastQC (version 0.11.5)