FastQCFastQC Report
Thu 26 May 2016
SRR936034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286492
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70120.21335819469513387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48600.14787804138881216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34730.10567498718998859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14850.043.893154100-101
GGTATCA83400.039.2458231
CGGTATC11250.034.922115100-101
CGTACAT19950.032.970726100-101
CTACGCG2100.032.592396-97
GTATCAA161650.029.562191
ACGTATC6150.029.52119100-101
TACGCAG11350.028.05867100-101
CCTGTCG9300.027.83830396-97
CGCTAGT2050.027.58075796-97
TCGTATC6700.027.542038100-101
CCGTACA7750.027.2669100-101
TCGGTAT4650.026.882862100-101
TACGCGC907.281542E-726.4517296-97
CCGTATC7600.026.238583100-101
ACCGTAC3300.026.15292498-99
AGTATCA35650.026.131475100-101
GCGTACA7950.025.457802100-101
TCGAGTA6300.025.038855100-101
GGGTATC16250.024.909481100-101
Produced by FastQC (version 0.11.5)