FastQCFastQC Report
Thu 26 May 2016
SRR936033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632963
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54800.2081305358259877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA1751.6916601E-1024.3426659
ATTCGCG2257.2759576E-1222.08829356
CGGTTAC2009.0039975E-1021.2957844
CGCGCTT2351.4551915E-1121.1451538
TCGCGGA2401.8189894E-1120.70777358
CGATCGT2653.6379788E-1220.0903631
CGCTCCG2507.185008E-1018.458125
TCGCGCT2701.0186341E-1018.4058637
CAATTCG2601.2114469E-917.7508754
ATCGTTC3253.6379788E-1217.4731313
AGTACGC2107.401559E-716.90623346
ACGCACG2351.3127283E-716.61909749
CTCGCGC3004.5474735E-1016.56464636
CGTTAGG1502.8409518E-416.56370229
TTAGTCG1754.9884387E-516.22628415
GTAGACC13750.016.005328
GCCTCGA7700.015.67460321
AATTCGC2956.4792403E-915.64364555
CGCACGG2301.9116815E-615.43671250
GACGCGA4950.015.07021969
Produced by FastQC (version 0.11.5)