##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936033_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2632963 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.747129754576875 40.0 36.0 41.0 30.0 41.0 2 36.610468889992 39.0 36.0 41.0 30.0 41.0 3 36.51941481897011 39.0 36.0 41.0 30.0 41.0 4 36.40225137990925 39.0 36.0 41.0 30.0 41.0 5 36.32851695979017 39.0 36.0 41.0 30.0 41.0 6 36.22409505944444 39.0 36.0 41.0 29.0 41.0 7 36.06752316686562 39.0 35.0 40.0 29.0 41.0 8 35.88440019855957 39.0 35.0 40.0 28.0 41.0 9 35.79990755661967 39.0 35.0 40.0 28.0 41.0 10-11 36.01375503567654 39.0 35.0 40.5 29.0 41.0 12-13 36.20665558156344 39.0 35.0 41.0 30.0 41.0 14-15 36.148617546087806 39.0 35.0 41.0 29.0 41.0 16-17 35.98631408796858 39.0 35.0 41.0 28.5 41.0 18-19 35.841936062147475 39.0 35.0 41.0 28.0 41.0 20-21 35.64715759393505 39.0 35.0 41.0 27.0 41.0 22-23 35.396760227925725 39.0 35.0 41.0 26.5 41.0 24-25 35.169931556197334 39.0 35.0 41.0 26.0 41.0 26-27 34.23824147927639 38.0 33.5 40.0 24.0 40.5 28-29 34.27239444686462 38.0 33.5 40.0 24.0 40.5 30-31 34.64811127235742 38.0 34.0 40.0 24.0 41.0 32-33 34.48755185697634 38.0 34.0 40.0 23.5 41.0 34-35 34.216615083463005 38.0 34.0 40.0 23.0 41.0 36-37 33.925199670485306 38.0 33.0 40.0 22.0 41.0 38-39 33.64195812854187 37.0 33.0 40.0 22.0 41.0 40-41 33.261907592320895 36.5 33.0 40.0 20.5 41.0 42-43 32.894463955627174 36.0 32.0 39.0 19.5 41.0 44-45 32.5244549581593 36.0 32.0 39.0 18.5 41.0 46-47 32.131984384133006 35.0 32.0 38.5 17.5 40.0 48-49 31.670392253897987 35.0 31.0 37.5 13.0 40.0 50-51 31.23111794582757 35.0 31.0 37.0 9.0 39.0 52-53 30.787804462121194 35.0 31.0 36.5 7.0 39.0 54-55 30.333476391426693 34.5 30.0 36.0 3.5 38.5 56-57 29.888796956128893 34.0 30.0 35.5 2.0 37.0 58-59 29.46001519960592 34.0 29.0 35.0 2.0 37.0 60-61 29.10664620049731 34.0 29.0 35.0 2.0 36.5 62-63 28.77660168411026 34.0 29.0 35.0 2.0 36.0 64-65 28.40863202407326 34.0 29.0 35.0 2.0 36.0 66-67 28.02247866756958 34.0 28.0 35.0 2.0 35.0 68-69 27.665585691861224 34.0 27.0 35.0 2.0 35.0 70-71 27.24983431214187 33.0 26.5 35.0 2.0 35.0 72-73 26.7724546452039 33.0 25.5 35.0 2.0 35.0 74-75 26.189070640187502 33.0 25.0 35.0 2.0 35.0 76-77 24.467799015785637 31.0 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6993441780314171 1101 2 0.7851381354478093 1101 3 0.8281002176608325 1101 4 0.8540282534751782 1101 5 0.9073418268752675 1101 6 0.7887230516785095 1101 7 0.9061868447557302 1101 8 1.063018012789776 1101 9 1.0431755875500812 1101 10-11 0.8094112089068233 1101 12-13 0.8395263999076761 1101 14-15 0.8518477638877187 1101 16-17 0.948360078090758 1101 18-19 0.9006899498014604 1101 20-21 0.8761453014438061 1101 22-23 0.9516522485877061 1101 24-25 0.7827886628289704 1101 26-27 0.7522273563331368 1101 28-29 0.8538696627968463 1101 30-31 0.942922360207973 1101 32-33 0.9746905376786188 1101 34-35 0.8125209670656446 1101 36-37 0.9745615736458646 1101 38-39 1.0595857669406072 1101 40-41 1.117393382482632 1101 42-43 1.0495850313335282 1101 44-45 0.8922531679017354 1101 46-47 0.8847295194918914 1101 48-49 0.9394148820295101 1101 50-51 0.9254926145728213 1101 52-53 1.019320406327978 1101 54-55 1.0084868413190016 1101 56-57 0.9564383400867662 1101 58-59 0.9840099895302217 1101 60-61 0.9983903729412162 1101 62-63 0.8424797619139923 1101 64-65 0.8249701459197354 1101 66-67 0.820062529637891 1101 68-69 0.8184629228372309 1101 70-71 0.7677347578340239 1101 72-73 0.7182978451568367 1101 74-75 0.7844363378522381 1101 76-77 0.8044714942276521 1102 1 0.8141806768942885 1102 2 0.8804196524433223 1102 3 0.7267175266533954 1102 4 0.8922235943074313 1102 5 0.8742294659434364 1102 6 0.8878373180747232 1102 7 0.7690524173170417 1102 8 0.8177626190962499 1102 9 0.879511563946906 1102 10-11 0.679255083760836 1102 12-13 0.9251049542134169 1102 14-15 0.7695521403091377 1102 16-17 0.9172570600810488 1102 18-19 1.0043289257680215 1102 20-21 1.0003407037426584 1102 22-23 1.0189778371303362 1102 24-25 1.0882762465772018 1102 26-27 0.9987088548886689 1102 28-29 1.0193286645793762 1102 30-31 1.1730654237029654 1102 32-33 1.054077254747888 1102 34-35 1.0670198606669672 1102 36-37 1.0761383454204037 1102 38-39 1.2122578734007519 1102 40-41 1.3490794111260627 1102 42-43 1.277689555274769 1102 44-45 1.205808549301942 1102 46-47 1.0142443944073776 1102 48-49 1.0827609696808693 1102 50-51 1.179300623669878 1102 52-53 1.2171589335884114 1102 54-55 1.2784050907504358 1102 56-57 1.3709502953390817 1102 58-59 1.1441384547093953 1102 60-61 1.0723763278247347 1102 62-63 1.1253235138348217 1102 64-65 1.0293457990022858 1102 66-67 1.0954602184939972 1102 68-69 1.1805633899833374 1102 70-71 1.1211785773681058 1102 72-73 1.1491388618142828 1102 74-75 1.1912055000625763 1102 76-77 1.4480738975714296 1103 1 0.5251589816824804 1103 2 0.4392454259793155 1103 3 0.5319772686783182 1103 4 0.5660565097915011 1103 5 0.6843492542056779 1103 6 0.4518655621316441 1103 7 0.5675135622775827 1103 8 0.6307343985163172 1103 9 0.5381928613101934 1103 10-11 0.582875853732034 1103 12-13 0.6212264161380574 1103 14-15 0.6120447697692981 1103 16-17 0.6774443755858641 1103 18-19 0.627862463268805 1103 20-21 0.7036370896450919 1103 22-23 0.6928635758462462 1103 24-25 0.4529983438633778 1103 26-27 0.311496139132025 1103 28-29 0.37480623293139814 1103 30-31 0.558072877647902 1103 32-33 0.4823307179131078 1103 34-35 0.5189152725213617 1103 36-37 0.4661045416411014 1103 38-39 0.424573980594225 1103 40-41 0.48782781561733657 1103 42-43 0.5141897443263375 1103 44-45 0.5476506189594161 1103 46-47 0.4477398682904621 1103 48-49 0.5033388465699886 1103 50-51 0.5093841183560457 1103 52-53 0.5136067720370541 1103 54-55 0.6128802502395239 1103 56-57 0.6130507509162904 1103 58-59 0.38548378519748283 1103 60-61 0.3512563486574791 1103 62-63 0.30904966460900596 1103 64-65 0.4167386388904326 1103 66-67 0.35197153131503 1103 68-69 0.3115561238543343 1103 70-71 0.1372948372855909 1103 72-73 0.1763030543355022 1103 74-75 0.2422657179290617 1103 76-77 0.5180794301099247 1104 1 0.5446338498031764 1104 2 0.5281536653501533 1104 3 0.4293846157046417 1104 4 0.43803843299655654 1104 5 0.5465087618275106 1104 6 0.22334323595237748 1104 7 0.5860663462044684 1104 8 0.5725695926085663 1104 9 0.6085851446489201 1104 10-11 0.4870368048840703 1104 12-13 0.46426677612071643 1104 14-15 0.48590807448577067 1104 16-17 0.6944509272529373 1104 18-19 0.6037574504530099 1104 20-21 0.37293445964031235 1104 22-23 0.4621133931706396 1104 24-25 0.5204420072003373 1104 26-27 0.3800941027189495 1104 28-29 0.3773276339446525 1104 30-31 0.4475354924037802 1104 32-33 0.4263635665461436 1104 34-35 0.6551420405145691 1104 36-37 0.5323784955670945 1104 38-39 0.5208746108597282 1104 40-41 0.5400090902199892 1104 42-43 0.5245619587737806 1104 44-45 0.5045570511438555 1104 46-47 0.3331610453469622 1104 48-49 0.48272092688685575 1104 50-51 0.4645356782408214 1104 52-53 0.5128840053406876 1104 54-55 0.4802716302932275 1104 56-57 0.5064141177316763 1104 58-59 0.4422161554993487 1104 60-61 0.30184957053737804 1104 62-63 0.427416829782576 1104 64-65 0.3104145034907546 1104 66-67 0.22634071878689 1104 68-69 0.24822115920448695 1104 70-71 0.25383894462210677 1104 72-73 0.39715832132540996 1104 74-75 0.2677434558519032 1104 76-77 0.13359320389666252 1105 1 0.05025967425354594 1105 2 0.1838967689248392 1105 3 0.05125694134321179 1105 4 0.12910821453761656 1105 5 0.09923168920317949 1105 6 -0.1067212537032205 1105 7 0.08853607759306925 1105 8 0.24124913502590317 1105 9 0.06524947620719956 1105 10-11 0.13523886384587058 1105 12-13 0.15064585478182835 1105 14-15 -0.07876272144115859 1105 16-17 0.10074361306503476 1105 18-19 0.25277032805205124 1105 20-21 0.25058186566760554 1105 22-23 0.24523404064258614 1105 24-25 0.2131961165906162 1105 26-27 -0.05377134574816722 1105 28-29 0.2579247316328903 1105 30-31 0.17993946492219948 1105 32-33 0.2908570421054506 1105 34-35 0.28202961213123245 1105 36-37 0.3061835084718041 1105 38-39 0.2614315927413813 1105 40-41 0.2991993436377811 1105 42-43 0.09775821077079172 1105 44-45 -0.05580041759866816 1105 46-47 -0.08833264154272769 1105 48-49 0.013128320123421133 1105 50-51 0.19861781737261808 1105 52-53 0.09268386991672273 1105 54-55 -0.006480942484831331 1105 56-57 0.12980041304833634 1105 58-59 0.10068175516319045 1105 60-61 -0.11523174730167796 1105 62-63 -0.04223400065959737 1105 64-65 0.14809859351626642 1105 66-67 0.2744008228748065 1105 68-69 -0.2205372162254875 1105 70-71 -0.32226987758988557 1105 72-73 -0.3010235287828138 1105 74-75 -0.5870961332922455 1105 76-77 -0.48151747346344465 1106 1 0.30234207140364333 1106 2 0.43558562022684555 1106 3 0.15406590972278167 1106 4 0.3047817780254931 1106 5 0.14160485440032744 1106 6 0.15876310075599065 1106 7 0.23946184070452148 1106 8 0.36782436508276106 1106 9 0.10359182015882595 1106 10-11 0.254496978751952 1106 12-13 0.3190485889485828 1106 14-15 0.24522948021061097 1106 16-17 0.5465249969508648 1106 18-19 0.502346777821927 1106 20-21 0.3829300318648947 1106 22-23 0.3830111392514084 1106 24-25 0.4933477294308162 1106 26-27 0.5026824805315755 1106 28-29 0.4851639656419522 1106 30-31 0.5790054215316758 1106 32-33 0.4764901996921864 1106 34-35 0.3361670535994321 1106 36-37 0.44661680896313527 1106 38-39 0.3652581515969544 1106 40-41 0.3416427516517828 1106 42-43 0.42362975666167557 1106 44-45 0.19966363622129535 1106 46-47 0.4336870859895683 1106 48-49 0.29254889596565903 1106 50-51 0.2303996683034022 1106 52-53 0.23174557474245105 1106 54-55 0.5128193181113687 1106 56-57 0.28524381268902843 1106 58-59 0.25116256957050354 1106 60-61 0.2196990286348992 1106 62-63 0.1826302353264957 1106 64-65 0.011294427698558707 1106 66-67 -0.03960573436192405 1106 68-69 -0.07207692946732891 1106 70-71 -0.18088736870254607 1106 72-73 -0.33326777282589504 1106 74-75 -0.34045034409326647 1106 76-77 -0.21663436960548665 1107 1 0.051713705902990625 1107 2 -0.01291203930487228 1107 3 0.037426975554318176 1107 4 0.06711397114592899 1107 5 -0.0381394834736426 1107 6 -0.21956970349002347 1107 7 -0.16382292954673972 1107 8 0.0903229710268505 1107 9 -0.19619707994591806 1107 10-11 -0.2116473776698058 1107 12-13 -0.15124887999279935 1107 14-15 -0.09996610969796649 1107 16-17 -0.08954407450683277 1107 18-19 0.03186316228887165 1107 20-21 -0.025710186975132387 1107 22-23 -0.08613846286304039 1107 24-25 -0.37672365871596725 1107 26-27 -0.34444242033886496 1107 28-29 -0.23456792976283936 1107 30-31 -0.05767061414189101 1107 32-33 0.02044212230038056 1107 34-35 0.09955935341715616 1107 36-37 0.015336557355361435 1107 38-39 -0.04923539294328094 1107 40-41 -0.03050144009896627 1107 42-43 0.052422837150722046 1107 44-45 -0.05620856422314091 1107 46-47 -0.07426742974097067 1107 48-49 0.0412432689734068 1107 50-51 -0.0023080192758833107 1107 52-53 -0.08592424742476013 1107 54-55 -0.1534326243524653 1107 56-57 -0.20126899360199957 1107 58-59 -0.12484789803567864 1107 60-61 -0.04266110505179199 1107 62-63 0.01923410111190549 1107 64-65 0.056377497379955344 1107 66-67 0.06588209009046864 1107 68-69 0.11753362362509634 1107 70-71 0.1192992386112266 1107 72-73 0.18876893691680152 1107 74-75 0.14552780162808077 1107 76-77 0.19804903702724985 1108 1 -0.18494592355138195 1108 2 -0.037001599964426646 1108 3 -0.09796049578117305 1108 4 -0.1372560011905435 1108 5 -0.1813801197346976 1108 6 -0.026111580969732984 1108 7 -0.16318594050295587 1108 8 -0.10199084617549659 1108 9 -0.0707329879283023 1108 10-11 -0.20372767013877535 1108 12-13 -0.14427896862527945 1108 14-15 -0.15172033202534152 1108 16-17 -0.1632249384459783 1108 18-19 -0.1837252108411107 1108 20-21 -0.2324114254638303 1108 22-23 -0.20452628909242776 1108 24-25 -0.15131200454641913 1108 26-27 -0.2840672337920722 1108 28-29 -0.12002990606683994 1108 30-31 0.03977107861100393 1108 32-33 0.03475446987800268 1108 34-35 0.026189021636866983 1108 36-37 0.1042181410921188 1108 38-39 -0.060023257392010976 1108 40-41 0.11811834114079289 1108 42-43 0.10305095384710938 1108 44-45 0.07137465384550268 1108 46-47 -0.025412076303048536 1108 48-49 -0.05986281700191398 1108 50-51 -0.06582663300572733 1108 52-53 0.09550558823206146 1108 54-55 0.17059630859988673 1108 56-57 0.0474507721680304 1108 58-59 -0.139734104492927 1108 60-61 -0.252722460746476 1108 62-63 -0.10017290758754882 1108 64-65 -0.173511279783078 1108 66-67 -0.2502726345482671 1108 68-69 -0.20177021559886654 1108 70-71 -0.04593344438662328 1108 72-73 -0.08974712885686742 1108 74-75 -0.17415936850258973 1108 76-77 -0.18307117645508342 1109 1 0.18855038275702185 1109 2 0.17557239038769268 1109 3 -0.04646495850634835 1109 4 0.26014195620965097 1109 5 0.27028262408389736 1109 6 0.32990028079065326 1109 7 0.12265743299102638 1109 8 0.26259020530314814 1109 9 0.344438529864334 1109 10-11 0.1720717315297975 1109 12-13 0.3299280383193306 1109 14-15 0.39775780295170193 1109 16-17 0.4120696392846739 1109 18-19 0.1972147724965012 1109 20-21 0.24561016282356007 1109 22-23 0.29827150750984543 1109 24-25 0.20515829153151088 1109 26-27 0.16571429075874278 1109 28-29 0.31002015394107474 1109 30-31 0.2871928498351579 1109 32-33 0.386358152500172 1109 34-35 0.4626836704448678 1109 36-37 0.5646654914218701 1109 38-39 0.5827332619153864 1109 40-41 0.64006186960669 1109 42-43 0.619661642248694 1109 44-45 0.4885329873730413 1109 46-47 0.1494816718034926 1109 48-49 0.44998984315515145 1109 50-51 0.4787095144855904 1109 52-53 0.48110710940379064 1109 54-55 0.45657156844002245 1109 56-57 0.46411877396243995 1109 58-59 0.4550302041924894 1109 60-61 0.20601619309519492 1109 62-63 0.32557529800076423 1109 64-65 0.29940599375983723 1109 66-67 0.3809951347882681 1109 68-69 0.40720832428601383 1109 70-71 0.3810039958967515 1109 72-73 0.2446459317802656 1109 74-75 0.2804946683411096 1109 76-77 0.31812075277052543 1110 1 -0.1685533459416746 1110 2 -0.12396734616089589 1110 3 -0.20300182899725172 1110 4 -0.09302341390710467 1110 5 -0.022497875429010605 1110 6 -0.3422029537601645 1110 7 -0.2820870925564236 1110 8 -0.22447017956746151 1110 9 -0.1052572162283667 1110 10-11 -0.20903805401002273 1110 12-13 -0.11570545925934539 1110 14-15 -0.16120003917510672 1110 16-17 -0.2487624351481159 1110 18-19 -0.21528328620827608 1110 20-21 -0.3173096863907503 1110 22-23 -0.5160792527719593 1110 24-25 -0.43202527734564455 1110 26-27 -0.24413231162164095 1110 28-29 -0.27883822327729746 1110 30-31 -0.3726308099652087 1110 32-33 -0.17238402937447717 1110 34-35 -0.05236525019498828 1110 36-37 -0.11147034277033896 1110 38-39 -0.12259471109002362 1110 40-41 -0.03371582032257692 1110 42-43 0.04672400371079988 1110 44-45 -0.13115315813232797 1110 46-47 -0.12436308105197114 1110 48-49 -0.14221815074564859 1110 50-51 -0.2304512812336732 1110 52-53 -0.2087918020590287 1110 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-0.6580945196448269 2314 20-21 -0.35187808262855214 2314 22-23 -0.18093647982225747 2314 24-25 -0.25324110579588677 2314 26-27 -0.08417069196627125 2314 28-29 -0.2103844994901749 2314 30-31 -0.3786450993370636 2314 32-33 -0.37014835618468567 2314 34-35 -0.4740251457540765 2314 36-37 -0.7316444190093065 2314 38-39 -0.46618031447541597 2314 40-41 -0.29871917769696665 2314 42-43 -0.14268239317433284 2314 44-45 -0.17818804101068508 2314 46-47 -0.23632185539293715 2314 48-49 -0.1981314773481273 2314 50-51 -0.1378901601121676 2314 52-53 -0.018368048272922266 2314 54-55 -0.10147215220560923 2314 56-57 -0.07667075530519796 2314 58-59 -0.20563561589924362 2314 60-61 -0.18421418708265946 2314 62-63 -0.16947518287000918 2314 64-65 -0.0889169045986904 2314 66-67 -0.17718536488722592 2314 68-69 -0.02932908609076179 2314 70-71 0.06830261965777495 2314 72-73 0.2846382767629656 2314 74-75 0.295083876814882 2314 76-77 0.18243531509682853 2315 1 -1.1467375722042235 2315 2 -1.11465768809488 2315 3 -1.1760239343696526 2315 4 -1.2005245629120438 2315 5 -1.024802556944607 2315 6 -1.0972617877548956 2315 7 -1.0688794661288128 2315 8 -1.197691692893443 2315 9 -0.9877854224463363 2315 10-11 -0.9487144299743235 2315 12-13 -1.0006607839706092 2315 14-15 -1.140780244661677 2315 16-17 -1.0598424256595536 2315 18-19 -1.0220227561339996 2315 20-21 -1.1026202142411492 2315 22-23 -1.067944841433139 2315 24-25 -1.2786292882377168 2315 26-27 -1.2372112247865132 2315 28-29 -1.104398023424558 2315 30-31 -1.407392533522028 2315 32-33 -1.3568361393428958 2315 34-35 -1.244622686604366 2315 36-37 -1.32901589525639 2315 38-39 -1.2873737562051417 2315 40-41 -1.3502523487956601 2315 42-43 -1.5170045049549188 2315 44-45 -1.477566285946157 2315 46-47 -1.3334708601860399 2315 48-49 -1.290449177338317 2315 50-51 -1.1140998290396915 2315 52-53 -1.2912577396223455 2315 54-55 -1.2623536275699756 2315 56-57 -1.295230251520497 2315 58-59 -1.1118382454983866 2315 60-61 -1.1603585484282775 2315 62-63 -1.2551554817569368 2315 64-65 -1.2943708606250652 2315 66-67 -1.2856889273430063 2315 68-69 -1.2263105315682274 2315 70-71 -1.2252568653555365 2315 72-73 -2.5630943515322677 2315 74-75 -3.092466100848359 2315 76-77 -2.8845558133778546 2316 1 -3.3412172352131293 2316 2 -3.333366304063844 2316 3 -2.660427344307351 2316 4 -2.753056467205546 2316 5 -3.3157288353518837 2316 6 -2.9348692531679603 2316 7 -3.3631130072605515 2316 8 -3.1493737741136627 2316 9 -2.746914127581256 2316 10-11 -2.8087195717073072 2316 12-13 -2.915265035534425 2316 14-15 -3.0846477966006276 2316 16-17 -4.1579195186536 2316 18-19 -3.9499232577457093 2316 20-21 -3.3018578432102856 2316 22-23 -3.4695131232213967 2316 24-25 -3.484040323482539 2316 26-27 -3.104911933345001 2316 28-29 -3.3972129306178793 2316 30-31 -3.611116670498646 2316 32-33 -3.6435424441933755 2316 34-35 -3.5874175271999924 2316 36-37 -3.5954744973863235 2316 38-39 -3.6390611069032666 2316 40-41 -3.6563048232147395 2316 42-43 -3.625088017131141 2316 44-45 -3.55726727234984 2316 46-47 -3.302992918539921 2316 48-49 -3.4577775892986473 2316 50-51 -3.7133258278985046 2316 52-53 -4.595322582943201 2316 54-55 -3.919576884171196 2316 56-57 -3.845842921474464 2316 58-59 -4.1203310204586785 2316 60-61 -3.959524906378057 2316 62-63 -3.7807324097471273 2316 64-65 -3.797160357224918 2316 66-67 -3.8929660497183782 2316 68-69 -3.728605149674795 2316 70-71 -3.453360321707226 2316 72-73 -3.5963715037147885 2316 74-75 -3.681060794963148 2316 76-77 -3.5050489460239227 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61515.0 3 5321.0 4 6701.0 5 7761.0 6 9273.0 7 9866.0 8 11633.0 9 11798.0 10 14199.0 11 15832.0 12 13190.0 13 11209.0 14 11429.0 15 11875.0 16 11996.0 17 12922.0 18 13512.0 19 14652.0 20 15303.0 21 16773.0 22 18829.0 23 20995.0 24 24750.0 25 29039.0 26 34236.0 27 40784.0 28 48110.0 29 57498.0 30 68845.0 31 83746.0 32 103229.0 33 130786.0 34 174099.0 35 235573.0 36 333156.0 37 445743.0 38 394715.0 39 101380.0 40 690.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.771872569039285 26.492343993096075 28.31799549056787 22.417787947296773 2 22.4430120727686 26.713702210612322 28.28225428746051 22.561031429158568 3 22.680157046743286 26.858130694008036 27.74341789384758 22.718294365401096 4 22.40670741041674 26.820942880118942 28.22505622873023 22.547293480734087 5 22.625917002294667 26.874107118099143 28.050232714319197 22.449743165286993 6 22.69745026115765 26.729279169947517 28.02084247318628 22.552428095708553 7 22.57699235793346 26.468300124518812 28.121422173464804 22.833285344082928 8 22.524939306535142 26.738818860159448 27.882830368650836 22.85341146465458 9 22.432156978641306 26.517875898714195 28.225029259136996 22.824937863507504 10-11 22.93109408417318 26.69628442161805 27.85485274062368 22.51776875358509 12-13 22.955108084525758 26.430747233737367 27.848512624581055 22.765632057155816 14-15 22.65881935126707 26.718114821113687 27.936917611734508 22.686148215884735 16-17 22.951056595345783 26.535211995156484 27.989198555452077 22.524532854045663 18-19 22.871069889564886 26.482205481033972 27.812520892719306 22.83420373668184 20-21 22.83714454492443 26.700571539281214 27.803325146778658 22.658958769015694 22-23 23.001276013640695 26.82221319798497 27.371366314683428 22.80514447369091 24-25 22.891288913504525 26.908082248137017 27.351467612393364 22.84916122596509 26-27 22.969047264482953 27.021436473155404 27.25571507588697 22.753801186474675 28-29 23.204236372669524 26.837412505070795 27.207903297117358 22.750447825142324 30-31 22.893008947558037 26.835039452401016 27.378790718909784 22.893160881131163 32-33 22.857445645949863 27.160606373112916 27.204383231545016 22.777564749392205 34-35 22.912669190232428 26.81058499789471 27.37613671089269 22.90060910098017 36-37 22.805334232805198 26.96674005263126 27.54455956266219 22.68336615190135 38-39 22.782287932209417 26.881881562483617 27.600644691249414 22.73518581405755 40-41 22.679609799196207 26.91951002119732 27.411125960987135 22.98975421861934 42-43 22.63264444468502 27.118919946584587 27.279865120574982 22.96857048815541 44-45 22.83155183809285 26.78326154173409 27.554318511596815 22.830868108576247 46-47 22.756768533321885 26.798884569337662 27.441828042014986 23.00251885532547 48-49 22.830669559892357 26.840214621426583 27.310401784561055 23.018714034120006 50-51 22.77101553675375 27.121576342402044 27.155048770581647 22.952359350262554 52-53 22.79313118433346 26.9368411973922 27.31319670222075 22.95683091605359 54-55 22.761736174441836 27.021290458243573 27.25267173092985 22.964301636384736 56-57 22.7719673060296 27.1986110076994 27.157033169579837 22.872388516691156 58-59 22.929210817709926 27.156675979616608 27.198384826792427 22.715728375881042 60-61 22.816557433283304 27.131475511094855 27.04896641401105 23.003000641610797 62-63 22.828602135259978 27.015335380482085 27.21151519272843 22.944547291529506 64-65 22.845721568854643 27.044213193582422 27.03010007398466 23.079965163578276 66-67 22.74143544799743 27.181833049955163 27.228143583930052 22.848587918117353 68-69 22.83507334493721 26.962276761203686 27.323235604676356 22.879414289182748 70-71 22.670536850217285 26.910702044253398 27.341129180581035 23.07763192494828 72-73 22.85218821890856 26.703067292501988 27.300469376491034 23.14427511209842 74-75 22.62232211890442 26.767711324436906 27.255315849133204 23.354650707525472 76-77 22.62095035209454 26.604981459655146 27.57047830459385 23.203589883656463 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5235.0 1 3178.5 2 1065.5 3 1065.0 4 1121.0 5 1160.5 6 1247.0 7 1371.0 8 1488.0 9 1528.0 10 1579.0 11 1731.0 12 1887.0 13 1942.0 14 2075.0 15 2394.5 16 2799.0 17 3017.0 18 3311.5 19 4003.0 20 5011.5 21 6520.5 22 7418.0 23 8904.0 24 11957.0 25 16043.5 26 18563.0 27 21042.5 28 28115.0 29 35256.5 30 37805.0 31 43395.0 32 54367.0 33 64956.5 34 75000.5 35 79837.0 36 85128.0 37 94686.5 38 100453.0 39 101952.0 40 105310.5 41 110760.0 42 116394.5 43 119938.0 44 120489.5 45 121995.5 46 123290.5 47 124587.5 48 125544.0 49 125367.5 50 123626.5 51 118971.5 52 115881.0 53 114011.5 54 107960.5 55 97076.0 56 85735.5 57 81098.0 58 77090.5 59 65820.5 60 53507.5 61 48457.0 62 42564.5 63 31540.5 64 22879.0 65 19349.0 66 16662.0 67 12388.5 68 9108.5 69 6289.0 70 5163.0 71 4400.5 72 3084.5 73 2263.0 74 1995.0 75 1766.5 76 1240.5 77 804.5 78 666.0 79 508.0 80 330.0 81 249.5 82 156.5 83 124.0 84 122.5 85 108.5 86 81.5 87 67.0 88 68.5 89 64.5 90 47.0 91 35.0 92 40.0 93 41.5 94 38.0 95 45.0 96 52.0 97 52.0 98 53.5 99 93.5 100 132.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00983682641951292 2 0.013065128526302877 3 0.01409058919551851 4 0.01564777021173484 5 0.012761288328016764 6 0.009950766493870214 7 0.016293430633092833 8 0.01792657169888069 9 0.017166971203165408 10-11 0.020243353210812304 12-13 0.016711210905736237 14-15 0.00818469534133218 16-17 0.0063426641392226165 18-19 0.01773667157495187 20-21 0.022712054821886977 22-23 0.02073709353302724 24-25 0.018610212145024445 26-27 0.023813475540674138 28-29 0.009077225923797637 30-31 0.008868335787475935 32-33 0.011147137274621787 34-35 0.011868757745551305 36-37 0.011659867609229602 38-39 0.014622309542519207 40-41 0.01209663789426589 42-43 0.014793219654055147 44-45 0.013027148501517111 46-47 0.023414685280423612 48-49 0.023053875044958854 50-51 0.03592910344733291 52-53 0.03835982503362182 54-55 0.027630468031643436 56-57 0.02092699365695606 58-59 0.016255450608307066 60-61 0.019977493037311955 62-63 0.025978336953462695 64-65 0.02508580637099724 66-67 0.027820368155572258 68-69 0.04117034686776837 70-71 0.05283021447699797 72-73 0.085037275495326 74-75 0.08526515564404058 76-77 0.08919608820936717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2632963.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.04938827323011 #Duplication Level Percentage of deduplicated Percentage of total 1 77.4164205104549 44.93975852935051 2 12.556198922384038 14.577593329627689 3 4.288881003781257 7.469007559385372 4 1.9561534171982944 4.542140369477987 5 1.0421106088562595 3.024694167857462 6 0.633407265836203 2.2061342585766495 7 0.41612838508175065 1.6909198737985929 8 0.2853583112157511 1.3251900323805097 9 0.20746489912479518 1.0838889434125576 >10 1.0827514422115725 11.42121086632501 >50 0.07898785176007478 3.1275126528790067 >100 0.034033604835728926 3.3526682837861026 >500 0.0014463468658706762 0.572031684933761 >1k 5.916873542056426E-4 0.45811383987062837 >5k 6.574303935618251E-5 0.2091356083381409 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5480 0.2081305358259877 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.1394007435729252E-4 7.596004957152834E-5 3 0.0 0.0 0.0 1.8990012392882088E-4 7.596004957152834E-5 4 0.0 0.0 0.0 7.596004957152835E-4 7.596004957152834E-5 5 0.0 0.0 0.0 0.001519200991430567 1.1394007435729252E-4 6 0.0 0.0 0.0 0.002582641685431964 1.1394007435729252E-4 7 0.0 0.0 0.0 0.005507103593935805 1.8990012392882088E-4 8 0.0 0.0 0.0 0.007785905081081656 1.8990012392882088E-4 9 0.0 0.0 0.0 0.01128006736137196 1.8990012392882088E-4 10 0.0 0.0 0.0 0.014888169716019556 2.2788014871458505E-4 11 0.0 0.0 0.0 0.019027992417667852 2.2788014871458505E-4 12 0.0 0.0 0.0 0.025522576656033524 2.2788014871458505E-4 13 0.0 0.0 0.0 0.03384020208411588 2.2788014871458505E-4 14 0.0 0.0 0.0 0.04436066894977256 2.658601735003492E-4 15 0.0 0.0 0.0 0.05522295603850111 2.658601735003492E-4 16 0.0 0.0 0.0 0.06680686359815918 3.038401982861134E-4 17 0.0 0.0 0.0 0.07763117066210197 3.418202230718776E-4 18 0.0 0.0 0.0 0.0893669983209031 3.418202230718776E-4 19 0.0 0.0 0.0 0.10216626667370562 3.418202230718776E-4 20 0.0 0.0 0.0 0.11420593453079288 3.418202230718776E-4 21 0.0 0.0 0.0 0.12837248377588292 4.1778027264340593E-4 22 0.0 0.0 0.0 0.1445139943098327 4.557602974291701E-4 23 0.0 0.0 0.0 0.1616809655129981 5.697003717864626E-4 24 0.0 0.0 0.0 0.17972147728623608 6.076803965722268E-4 25 0.0 0.0 0.0 0.19859754960476086 6.076803965722268E-4 26 0.0 0.0 0.0 0.2179673622455006 6.076803965722268E-4 27 0.0 0.0 0.0 0.2376789951093122 6.076803965722268E-4 28 0.0 0.0 0.0 0.25647910737826546 6.076803965722268E-4 29 0.0 0.0 0.0 0.2751272995480757 6.45660421357991E-4 30 0.0 0.0 0.0 0.2958643930811029 6.45660421357991E-4 31 0.0 0.0 0.0 0.3185764479029899 6.45660421357991E-4 32 0.0 0.0 0.0 0.3429596238154505 6.45660421357991E-4 33 0.0 0.0 0.0 0.3680644201988406 6.45660421357991E-4 34 0.0 0.0 0.0 0.3946124575240898 6.45660421357991E-4 35 0.0 0.0 0.0 0.4225277757416264 6.45660421357991E-4 36 0.0 0.0 0.0 0.4535574559915958 6.45660421357991E-4 37 0.0 0.0 0.0 0.48466309629113663 6.45660421357991E-4 38 0.0 0.0 0.0 0.5147432759214619 6.45660421357991E-4 39 0.0 0.0 0.0 0.5480137776337913 6.45660421357991E-4 40 0.0 0.0 0.0 0.5797270983299043 6.45660421357991E-4 41 0.0 0.0 0.0 0.6165677223720957 6.45660421357991E-4 42 0.0 0.0 0.0 0.6548895673809316 6.45660421357991E-4 43 0.0 0.0 0.0 0.6979589154879883 7.216204709295193E-4 44 0.0 0.0 0.0 0.7432690850574049 7.216204709295193E-4 45 0.0 0.0 0.0 0.7865663133131761 7.216204709295193E-4 46 0.0 0.0 0.0 0.8367759060799563 7.975805205010476E-4 47 0.0 0.0 0.0 0.8933661430107449 8.355605452868119E-4 48 0.0 0.0 0.0 0.9454367569920276 9.874806444298686E-4 49 0.0 0.0 0.0 1.000925573204029 0.0010254606692156327 50 0.0 0.0 0.0 1.060174411869821 0.0010254606692156327 51 0.0 0.0 0.0 1.1235250932124758 0.0010254606692156327 52 0.0 0.0 0.0 1.1869137545799162 0.0010254606692156327 53 0.0 0.0 0.0 1.2540624384011474 0.0010254606692156327 54 0.0 0.0 0.0 1.32899702730346 0.0010254606692156327 55 0.0 0.0 0.0 1.4089449794774935 0.0010254606692156327 56 0.0 0.0 0.0 1.498084097649682 0.0010254606692156327 57 0.0 0.0 0.0 1.5835771334424373 0.0011773807683586894 58 0.0 0.0 0.0 1.6756786935479153 0.0012153607931444535 59 0.0 0.0 0.0 1.7695653148183244 0.0012153607931444535 60 0.0 0.0 0.0 1.868351359286097 0.0012153607931444535 61 0.0 0.0 0.0 1.9762146296776673 0.0012153607931444535 62 0.0 0.0 0.0 2.082254858879521 0.0012533408179302178 63 0.0 0.0 0.0 2.1992333352196747 0.0012533408179302178 64 0.0 0.0 0.0 2.3235799363682665 0.0012533408179302178 65 0.0 0.0 0.0 2.4558263826722975 0.0012533408179302178 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 175 1.6916601E-10 24.34266 59 ATTCGCG 225 7.2759576E-12 22.088293 56 CGGTTAC 200 9.0039975E-10 21.295784 4 CGCGCTT 235 1.4551915E-11 21.14515 38 TCGCGGA 240 1.8189894E-11 20.707773 58 CGATCGT 265 3.6379788E-12 20.090363 1 CGCTCCG 250 7.185008E-10 18.4581 25 TCGCGCT 270 1.0186341E-10 18.40586 37 CAATTCG 260 1.2114469E-9 17.75087 54 ATCGTTC 325 3.6379788E-12 17.473131 3 AGTACGC 210 7.401559E-7 16.906233 46 ACGCACG 235 1.3127283E-7 16.619097 49 CTCGCGC 300 4.5474735E-10 16.564646 36 CGTTAGG 150 2.8409518E-4 16.563702 29 TTAGTCG 175 4.9884387E-5 16.226284 15 GTAGACC 1375 0.0 16.00532 8 GCCTCGA 770 0.0 15.674603 21 AATTCGC 295 6.4792403E-9 15.643645 55 CGCACGG 230 1.9116815E-6 15.436712 50 GACGCGA 495 0.0 15.070219 69 >>END_MODULE