FastQCFastQC Report
Thu 26 May 2016
SRR936033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632963
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63200.2400337566460296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45870.17421437369230028No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33500.12723308303230998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA10400.044.355927100-101
GGTATCA67250.037.3397521
GCGTATC2700.030.86367100-101
ACGTATC3500.030.611721100-101
GTATCAA127800.030.5438841
CGTACAT18050.030.50337100-101
CGGTATC9050.028.939257100-101
CCGTACA6800.028.886059100-101
TACGCAG8000.028.645344100-101
TCGTATC4950.027.657053100-101
GCGTACA6150.027.583733100-101
CGAGTAC11100.026.543947100-101
TCGTACA8900.026.417418100-101
AGTATCA28550.025.643795100-101
ACCGTAC2750.024.88992998-99
CGCATTA1800.024.801163100-101
AGGTATC20350.024.569605100-101
GGGTATC13100.023.627367100-101
TCGGTAT3300.023.448372100-101
TATCAAC166250.023.3334792
Produced by FastQC (version 0.11.5)