FastQCFastQC Report
Thu 26 May 2016
SRR936032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646965
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54140.2045361385586889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA1900.028.02916559
CGGTTAC2850.023.661474
CAATTCG2351.4551915E-1121.1474954
AATTCGC2201.2914825E-1020.97623455
TCGCGGA2401.8189894E-1120.71004958
CTCGCGC2601.8189894E-1220.4766636
ATTCGCG2302.3646862E-1020.06498156
TCGCGCT2503.45608E-1119.87788837
CGCGCTT2900.019.58560838
CCTCGCG3051.8189894E-1218.61815835
GACGCGA3950.017.08543669
CCCGTAT2401.6748527E-716.2672622
CGATTCG2008.720213E-615.97451115
TTAGTCG2008.720213E-615.97451115
ATCGTAC2604.1573003E-715.01536733
GCGGTTA4301.8189894E-1214.85720353
CGATCGT3402.6793714E-914.61416151
ACGCGAC4750.014.208158570
TAGTCGG2252.6578982E-514.19902916
TCGAACT8550.014.11599324
Produced by FastQC (version 0.11.5)