##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936032_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2646965 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.726813539279895 40.0 36.0 41.0 30.0 41.0 2 36.59769925178459 39.0 36.0 41.0 30.0 41.0 3 36.464505197461996 39.0 36.0 41.0 30.0 41.0 4 36.360154365471395 39.0 36.0 41.0 30.0 41.0 5 36.286817921657445 39.0 36.0 41.0 30.0 41.0 6 36.193569994314245 39.0 36.0 41.0 29.0 41.0 7 36.05550658962245 39.0 35.0 40.0 29.0 41.0 8 35.92920495737571 39.0 35.0 40.0 29.0 41.0 9 36.10632025735134 39.0 35.0 41.0 29.0 41.0 10-11 36.32758612221922 39.0 36.0 41.0 30.0 41.0 12-13 36.24863740170346 40.0 35.5 41.0 29.5 41.0 14-15 36.099517182886814 39.0 35.0 41.0 29.0 41.0 16-17 35.95427234587537 39.0 35.0 41.0 28.0 41.0 18-19 35.80050926249497 39.0 35.0 41.0 27.5 41.0 20-21 35.60263112659215 39.0 35.0 41.0 27.0 41.0 22-23 35.34640786712329 39.0 35.0 41.0 26.0 41.0 24-25 35.12577253571543 39.0 35.0 41.0 25.5 41.0 26-27 34.175620002531204 38.0 33.5 40.0 23.5 40.5 28-29 34.19212003181002 38.0 33.5 39.5 23.5 40.5 30-31 34.59397800877609 38.0 34.0 40.0 24.0 41.0 32-33 34.46938833720884 38.0 34.0 40.0 23.5 41.0 34-35 34.21652213006217 38.0 34.0 40.0 23.0 41.0 36-37 33.91088831926376 38.0 33.0 40.0 22.0 41.0 38-39 33.59229740476357 37.0 33.0 40.0 22.0 41.0 40-41 33.2364481585514 36.5 33.0 40.0 20.5 41.0 42-43 32.868293120611725 36.0 32.0 39.0 19.5 41.0 44-45 32.50262564862021 36.0 32.0 39.0 18.0 40.5 46-47 32.0854374727282 35.0 31.5 38.5 17.0 40.0 48-49 31.669595177873525 35.0 31.0 37.5 12.5 40.0 50-51 31.21785743294679 35.0 31.0 37.0 8.0 39.0 52-53 30.737031657010952 35.0 30.5 36.5 7.0 39.0 54-55 30.27552347688768 34.5 30.0 36.0 2.0 38.5 56-57 29.844851556405168 34.0 30.0 35.5 2.0 37.0 58-59 29.420951731511373 34.0 29.0 35.0 2.0 37.0 60-61 29.064026347156084 34.0 29.0 35.0 2.0 36.5 62-63 28.69680124217736 34.0 29.0 35.0 2.0 36.0 64-65 28.321448338002202 34.0 29.0 35.0 2.0 36.0 66-67 27.97133320614364 34.0 27.5 35.0 2.0 35.0 68-69 27.60494434191612 34.0 27.0 35.0 2.0 35.0 70-71 27.159083894195806 33.0 26.0 35.0 2.0 35.0 72-73 26.68500943533443 33.0 25.0 35.0 2.0 35.0 74-75 26.10734161577505 33.0 24.5 35.0 2.0 35.0 76-77 24.32471868725125 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9194737456811879 1101 2 0.8221721005833658 1101 3 0.8888055139921036 1101 4 0.8553946938654562 1101 5 0.8644347700082662 1101 6 0.8676680385437123 1101 7 0.8906323563800669 1101 8 0.779591331600983 1101 9 0.7587082957298108 1101 10-11 0.9294491857866944 1101 12-13 1.0670313542521583 1101 14-15 0.9999182409116685 1101 16-17 0.9890930227152026 1101 18-19 1.0470148832021522 1101 20-21 1.0277657186434155 1101 22-23 1.0328455372499477 1101 24-25 1.137103565578677 1101 26-27 1.1226858699821634 1101 28-29 1.2124618310574746 1101 30-31 1.1377476191259603 1101 32-33 1.1480627005682535 1101 34-35 1.179043687777245 1101 36-37 1.0272884710841268 1101 38-39 0.9805905099944496 1101 40-41 1.0096651945088198 1101 42-43 0.8812443647912573 1101 44-45 1.0219753427992018 1101 46-47 1.0730084427836104 1101 48-49 1.0530692398105792 1101 50-51 0.9350087216743042 1101 52-53 1.0785367997631852 1101 54-55 1.0791674508496456 1101 56-57 0.9884798549338711 1101 58-59 1.0871178721236952 1101 60-61 1.0668405992989811 1101 62-63 0.9879296560983697 1101 64-65 0.9535608931879764 1101 66-67 1.0148151102095326 1101 68-69 0.9926945602995687 1101 70-71 0.8942006066079387 1101 72-73 0.8583418362366793 1101 74-75 1.0894770592166374 1101 76-77 1.2471577385055461 1102 1 0.5625478006766471 1102 2 0.8128201737798193 1102 3 0.8334071525723274 1102 4 0.7161196674960877 1102 5 0.8311284192834094 1102 6 0.7153469209731256 1102 7 0.8256324790282861 1102 8 0.8679839040247543 1102 9 0.834009397110826 1102 10-11 0.9335309185607557 1102 12-13 0.8799627693673244 1102 14-15 0.8091082720643215 1102 16-17 0.896729407536256 1102 18-19 0.9091966110702856 1102 20-21 0.8453589926150045 1102 22-23 0.8884554668498694 1102 24-25 0.7658618136714992 1102 26-27 0.6002495244085395 1102 28-29 0.8175658367483578 1102 30-31 0.9033325284889244 1102 32-33 0.8166463589193711 1102 34-35 0.889861874055363 1102 36-37 0.9390571810701474 1102 38-39 0.9562794863724022 1102 40-41 0.8895285030672184 1102 42-43 0.9379240934933932 1102 44-45 0.8167517550934562 1102 46-47 0.9261681675610021 1102 48-49 0.9745703927038498 1102 50-51 0.8747050446806561 1102 52-53 0.815604464786361 1102 54-55 0.9207770860938389 1102 56-57 0.9639569565111259 1102 58-59 0.9890400763575116 1102 60-61 0.780894858871676 1102 62-63 0.8061996416258772 1102 64-65 0.6557584426765359 1102 66-67 0.7421285622663412 1102 68-69 0.6347733249866927 1102 70-71 0.7845181428504873 1102 72-73 0.7668146819206889 1102 74-75 0.7011853649541173 1102 76-77 0.7108136979825765 1103 1 0.5516879948626965 1103 2 0.3809806771659012 1103 3 0.43903398601653265 1103 4 0.40873127358412376 1103 5 0.2312118444025515 1103 6 0.3957146639874125 1103 7 0.4426339366959411 1103 8 0.43958682953829964 1103 9 0.3529263609454034 1103 10-11 0.38109305119033365 1103 12-13 0.42996467778507963 1103 14-15 0.5427517687218568 1103 16-17 0.5829853009120427 1103 18-19 0.5195806301617267 1103 20-21 0.5779819401571444 1103 22-23 0.4595263073170486 1103 24-25 0.5604058744936751 1103 26-27 0.5555550620916065 1103 28-29 0.49196841111303513 1103 30-31 0.6256023096933703 1103 32-33 0.6012091676740994 1103 34-35 0.699905694962176 1103 36-37 0.7058471678748077 1103 38-39 0.6858837259678907 1103 40-41 0.6260548465024343 1103 42-43 0.8513943876078329 1103 44-45 0.6607840418868491 1103 46-47 0.6081787401953775 1103 48-49 0.4856880621077302 1103 50-51 0.533092358973434 1103 52-53 0.5485107214885439 1103 54-55 0.5467503791504704 1103 56-57 0.5415759418144894 1103 58-59 0.5564283521293305 1103 60-61 0.49674463186083884 1103 62-63 0.3717135785281336 1103 64-65 0.5130158373409621 1103 66-67 0.4303005979483032 1103 68-69 0.4219134064852206 1103 70-71 0.3575055800090148 1103 72-73 0.3448632884122169 1103 74-75 0.4678369914713123 1103 76-77 0.29632876176007983 1104 1 0.5017534261190519 1104 2 0.39457611236562684 1104 3 0.5922431681953668 1104 4 0.5572380188131589 1104 5 0.5376871835257759 1104 6 0.4175691970926678 1104 7 0.42325578710174483 1104 8 0.5037018342241382 1104 9 0.43358288969275804 1104 10-11 0.5450282937615256 1104 12-13 0.6239473798873618 1104 14-15 0.6940262762826848 1104 16-17 0.6656655553873705 1104 18-19 0.5288699843594884 1104 20-21 0.4440939328069504 1104 22-23 0.4618712451091298 1104 24-25 0.43079860110407964 1104 26-27 0.24115529573301586 1104 28-29 0.30767702690886267 1104 30-31 0.46119938056235554 1104 32-33 0.5341476255062645 1104 34-35 0.5280200051839401 1104 36-37 0.5100251229472832 1104 38-39 0.4374165490406057 1104 40-41 0.517825895874438 1104 42-43 0.6443267800948789 1104 44-45 0.633390395156411 1104 46-47 0.45152034159902854 1104 48-49 0.35093287190846567 1104 50-51 0.4705726477233405 1104 52-53 0.49931144125479676 1104 54-55 0.4685089972566914 1104 56-57 0.35828318617836175 1104 58-59 0.33441025209869224 1104 60-61 0.3879488496085237 1104 62-63 0.25383503168074384 1104 64-65 0.3364952279532254 1104 66-67 0.3984889394756834 1104 68-69 0.1742230154364499 1104 70-71 0.16981522484985945 1104 72-73 0.19157156011634768 1104 74-75 0.059768467427286254 1104 76-77 0.07257898775250382 1105 1 0.01979482787822917 1105 2 0.2684193398766226 1105 3 0.1263097150675776 1105 4 0.2124927109578323 1105 5 0.07762374638621594 1105 6 0.14378012323739142 1105 7 0.09041930183810365 1105 8 0.21822562197932172 1105 9 0.1912996391137156 1105 10-11 0.29928366341297163 1105 12-13 0.4581607833860133 1105 14-15 0.5088740335945943 1105 16-17 0.36540045741848814 1105 18-19 0.2955850171564407 1105 20-21 0.08845115411489246 1105 22-23 0.26154938543508166 1105 24-25 0.1519486659243583 1105 26-27 0.00841391636203781 1105 28-29 0.23217267703405042 1105 30-31 0.5518353255788142 1105 32-33 0.43265435603205304 1105 34-35 0.27151446029501614 1105 36-37 0.2587161165613736 1105 38-39 0.07918344978703118 1105 40-41 0.3122440546970253 1105 42-43 0.2860311902185657 1105 44-45 0.11761462645581844 1105 46-47 0.1824758664440651 1105 48-49 0.05487750272588343 1105 50-51 0.13505463342771407 1105 52-53 0.08553729188916748 1105 54-55 -0.01981858957670468 1105 56-57 0.017662625723190217 1105 58-59 0.07545958760285743 1105 60-61 0.05295874554441937 1105 62-63 -0.015327977855925212 1105 64-65 0.12075979610712295 1105 66-67 0.2004945441446182 1105 68-69 0.2983633114998767 1105 70-71 0.1436313608810309 1105 72-73 -0.3438781320752078 1105 74-75 -0.5219555675358123 1105 76-77 -0.49998192924939744 1106 1 0.007025016601218681 1106 2 -0.013805864148231706 1106 3 0.0424221721759892 1106 4 0.11341690876920296 1106 5 0.09121690227975421 1106 6 -0.115200737840226 1106 7 -0.3167925710173165 1106 8 -0.19771324011842495 1106 9 0.03963701043895185 1106 10-11 -0.1266973367725086 1106 12-13 0.22038155334798404 1106 14-15 0.07491824091167132 1106 16-17 0.01270683667286221 1106 18-19 0.06907410298599359 1106 20-21 0.0930856192451941 1106 22-23 0.04014546838973132 1106 24-25 -0.15670949391056155 1106 26-27 -0.1861746431761233 1106 28-29 -0.002317666562355214 1106 30-31 0.1518430054916422 1106 32-33 -0.007639973004394562 1106 34-35 0.0026762137495310867 1106 36-37 0.04328499813421871 1106 38-39 0.1806616156499743 1106 40-41 0.1773323302233223 1106 42-43 0.2170105095174648 1106 44-45 0.18081190457878904 1106 46-47 0.197251728912768 1106 48-49 3.851884348726742E-4 1106 50-51 -0.0438938560928257 1106 52-53 0.008154924969012711 1106 54-55 0.05980051572794309 1106 56-57 -0.04705426081116926 1106 58-59 -0.13499972914572567 1106 60-61 -0.2870537451817228 1106 62-63 -0.12053640779193486 1106 64-65 -0.028963582127133236 1106 66-67 -0.268391230917981 1106 68-69 -0.11608811087793924 1106 70-71 -0.17304288909578247 1106 72-73 -0.31441421897124755 1106 74-75 -0.19725537394404924 1106 76-77 -0.16443697422760195 1107 1 -0.2353304640020255 1107 2 -0.05123999253850542 1107 3 -0.1063761419658249 1107 4 -0.3248089586884433 1107 5 -0.13058492975439862 1107 6 -0.39536119013254734 1107 7 -0.18073261043363686 1107 8 -0.24238023478044823 1107 9 -0.20384095367684552 1107 10-11 -0.2868857160678502 1107 12-13 -0.16051091631481285 1107 14-15 -0.2866374808534786 1107 16-17 -0.29180542191639347 1107 18-19 -0.2825865867711741 1107 20-21 -0.23051966584059613 1107 22-23 -0.32525035848559725 1107 24-25 -0.2926130405261844 1107 26-27 -0.5050737163522641 1107 28-29 -0.5053292042254114 1107 30-31 -0.37866767189459694 1107 32-33 -0.23025416231079987 1107 34-35 -0.3150119309248609 1107 36-37 -0.2800019134293237 1107 38-39 -0.09051370734487563 1107 40-41 -0.2214689700033361 1107 42-43 -0.2115516764104015 1107 44-45 -0.23993697706134043 1107 46-47 -0.2538226928532339 1107 48-49 -0.14106735698916495 1107 50-51 -0.10570367945880221 1107 52-53 -0.07108083638308216 1107 54-55 -0.09736082670204738 1107 56-57 -0.1638551117031568 1107 58-59 -0.03806572730857383 1107 60-61 -0.22699902873180733 1107 62-63 -0.13948084101881264 1107 64-65 -0.19061045057729586 1107 66-67 -0.18048771842903477 1107 68-69 -0.3541773294053172 1107 70-71 -0.2710821689090004 1107 72-73 -0.3888479293096445 1107 74-75 -0.4600585682016636 1107 76-77 -0.1601563505055772 1108 1 0.11435404968565877 1108 2 0.13928503836105222 1108 3 0.12168421493808523 1108 4 0.08885536847629538 1108 5 0.17058420043235145 1108 6 0.26615262928806516 1108 7 0.27780529352344985 1108 8 0.0895575405117981 1108 9 0.05384203290317657 1108 10-11 0.042307387189659096 1108 12-13 0.1942493269597989 1108 14-15 0.13398085533862059 1108 16-17 0.30363969737793894 1108 18-19 0.20261319018784718 1108 20-21 0.21225713680237135 1108 22-23 0.243010614793576 1108 24-25 0.3033283339857391 1108 26-27 0.15868448028037818 1108 28-29 0.4274047293065095 1108 30-31 0.5573376570976905 1108 32-33 0.544839933101116 1108 34-35 0.5701980340551884 1108 36-37 0.5637953893195018 1108 38-39 0.5805242685034884 1108 40-41 0.47476032565111836 1108 42-43 0.465171324717673 1108 44-45 0.42314044340842116 1108 46-47 0.41948156992979335 1108 48-49 0.4728756618474428 1108 50-51 0.35729346926467187 1108 52-53 0.511957902471373 1108 54-55 0.5035923968617482 1108 56-57 0.5752130321900424 1108 58-59 0.6729906307970808 1108 60-61 0.6173786762630868 1108 62-63 0.6396089031221841 1108 64-65 0.6107112260532688 1108 66-67 0.6719856975459422 1108 68-69 0.5535033840409795 1108 70-71 0.44020766467844297 1108 72-73 0.7447914508971678 1108 74-75 0.6718917093260508 1108 76-77 0.4365007872575539 1109 1 -0.12675672597264764 1109 2 -0.08419241299719715 1109 3 -0.15636746073000296 1109 4 -0.06696185532746313 1109 5 -0.0019301225512151632 1109 6 -0.14359579783068455 1109 7 -0.1700074766359947 1109 8 -0.062335834482155406 1109 9 0.0446749980593637 1109 10-11 -0.11154642097312006 1109 12-13 -0.051445033903881665 1109 14-15 0.07595489400311806 1109 16-17 0.1389111857169496 1109 18-19 0.04927345176963627 1109 20-21 0.030572932069759418 1109 22-23 0.109876718468918 1109 24-25 0.019321000357862772 1109 26-27 -0.07128676190978211 1109 28-29 0.12303782070260638 1109 30-31 0.14973457642086885 1109 32-33 0.10823719120601538 1109 34-35 0.22965136589005652 1109 36-37 0.2935516223812584 1109 38-39 0.21236519536906684 1109 40-41 0.24261099760887106 1109 42-43 0.23099737257198427 1109 44-45 0.30693221208354515 1109 46-47 0.15635960756072365 1109 48-49 0.11101482426894549 1109 50-51 0.05716011208963678 1109 52-53 0.052450587890646716 1109 54-55 0.1489871006148249 1109 56-57 0.05975711590749455 1109 58-59 0.08041971133751957 1109 60-61 0.006529676252107208 1109 62-63 -0.17855512498300286 1109 64-65 -0.028103669659845565 1109 66-67 -0.03460716558799959 1109 68-69 -0.04712956433202109 1109 70-71 -0.051956838724354526 1109 72-73 -0.032990988876214544 1109 74-75 0.06193534967190928 1109 76-77 0.01468602345104486 1110 1 -0.2927615859247297 1110 2 -0.3708950200201713 1110 3 -0.6321495143929283 1110 4 -0.43706866834206437 1110 5 -0.6111852702982006 1110 6 -0.5105267797557573 1110 7 -0.4147679270509599 1110 8 -0.7049436486510885 1110 9 -0.6515256886004224 1110 10-11 -0.545125038761114 1110 12-13 -0.3977946878599923 1110 14-15 -0.4314360366886163 1110 16-17 -0.519421158619167 1110 18-19 -0.5650039618863048 1110 20-21 -0.5576908317159095 1110 22-23 -0.5588218282111583 1110 24-25 -0.5189657436490478 1110 26-27 -0.5543423808794472 1110 28-29 -0.3633753945608831 1110 30-31 -0.2912661862569905 1110 32-33 -0.3135590196400386 1110 34-35 -0.28064537664879907 1110 36-37 -0.21869374040461054 1110 38-39 -0.31992303403938394 1110 40-41 -0.20948959964031388 1110 42-43 -0.34902426576730505 1110 44-45 -0.3076423607386545 1110 46-47 -0.4624430762557452 1110 48-49 -0.5678744681045274 1110 50-51 -0.4716761555754623 1110 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18-19 -0.5605805336498122 2314 20-21 -0.4131647755099337 2314 22-23 -0.24126503904430052 2314 24-25 -0.1751050827588827 2314 26-27 -0.09360235561754138 2314 28-29 -0.3863714543167305 2314 30-31 -0.59119919856834 2314 32-33 -0.6997928727176443 2314 34-35 -0.6992059786888589 2314 36-37 -1.0865420675403996 2314 38-39 -0.6668517428634786 2314 40-41 -0.5611603999662904 2314 42-43 -0.5189197862014936 2314 44-45 -0.41989297017330074 2314 46-47 -0.3402727459050148 2314 48-49 -0.2451506048018679 2314 50-51 -0.20176306266660404 2314 52-53 -0.14990186924040216 2314 54-55 -0.2503986934985889 2314 56-57 -0.3673478323029009 2314 58-59 -0.2600935229635475 2314 60-61 -0.3562262121170363 2314 62-63 -0.3455878838603006 2314 64-65 -0.24015820587812797 2314 66-67 -0.176164433045237 2314 68-69 -0.20588890165140938 2314 70-71 -0.07870336179136217 2314 72-73 -0.15427817056479753 2314 74-75 -0.12416251490636654 2314 76-77 -0.046408981352143996 2315 1 -0.9021469224074039 2315 2 -0.8028205492661584 2315 3 -0.719413557911075 2315 4 -0.7761548247665075 2315 5 -0.8493110804976922 2315 6 -0.6932040367645911 2315 7 -1.3178996982943616 2315 8 -1.2309701664398673 2315 9 -0.9096855226599985 2315 10-11 -1.0014783764757595 2315 12-13 -0.9399903113186738 2315 14-15 -0.9648645153680349 2315 16-17 -0.9030493991460133 2315 18-19 -0.8779855177151461 2315 20-21 -0.7443309719179254 2315 22-23 -0.8906977965644884 2315 24-25 -0.9514259232250382 2315 26-27 -0.8604063784155045 2315 28-29 -0.9407449727763648 2315 30-31 -0.8743638251919705 2315 32-33 -0.943033327143155 2315 34-35 -0.991348035151475 2315 36-37 -1.057362009214792 2315 38-39 -1.012873602561747 2315 40-41 -0.999214589102742 2315 42-43 -1.0846326284359158 2315 44-45 -1.1089498139594625 2315 46-47 -0.9483746149859016 2315 48-49 -0.8103839410672684 2315 50-51 -0.9333611753166764 2315 52-53 -0.9474470512880231 2315 54-55 -0.9797509544617036 2315 56-57 -1.0968509820210848 2315 58-59 -0.9167092844104516 2315 60-61 -1.0024824117503002 2315 62-63 -0.9499178723801229 2315 64-65 -0.7062225580150425 2315 66-67 -0.7644640137594436 2315 68-69 -1.0169982835939244 2315 70-71 -1.013517051393567 2315 72-73 -0.7262152303316292 2315 74-75 -0.8376035679249298 2315 76-77 -1.0986693806096532 2316 1 -1.5669046519343652 2316 2 -1.2773949026937714 2316 3 -1.5888410301898759 2316 4 -2.242287587374811 2316 5 -1.7591464984348733 2316 6 -1.7198551312295578 2316 7 -1.4455495615581597 2316 8 -1.3568211719208207 2316 9 -1.6063183217057215 2316 10-11 -1.4789794216543228 2316 12-13 -2.091565558421898 2316 14-15 -3.1085092543802375 2316 16-17 -2.2943993479418623 2316 18-19 -1.9033751104215213 2316 20-21 -1.5076977509652565 2316 22-23 -1.9708775672772418 2316 24-25 -1.835678438365747 2316 26-27 -1.6782342480539256 2316 28-29 -2.2230060871476596 2316 30-31 -2.28434633523144 2316 32-33 -2.4188645797081563 2316 34-35 -2.400888693556439 2316 36-37 -2.4279504926894617 2316 38-39 -2.428066088015548 2316 40-41 -2.179334909291761 2316 42-43 -2.289408615026943 2316 44-45 -2.1161283688131185 2316 46-47 -2.081731536752848 2316 48-49 -1.9019183782625895 2316 50-51 -1.9954645348465938 2316 52-53 -2.1488936167737336 2316 54-55 -2.095440331228442 2316 56-57 -2.050847591423473 2316 58-59 -2.1262509016948314 2316 60-61 -1.9509156027859262 2316 62-63 -1.8737801619540342 2316 64-65 -1.782940582263187 2316 66-67 -1.8017814910627692 2316 68-69 -1.9547267366145853 2316 70-71 -1.892178532440358 2316 72-73 -1.9912922667038409 2316 74-75 -2.256713459341423 2316 76-77 -2.830648584432897 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61655.0 3 5230.0 4 6566.0 5 8433.0 6 9707.0 7 10150.0 8 11750.0 9 12075.0 10 14543.0 11 16043.0 12 13220.0 13 11935.0 14 11380.0 15 11996.0 16 12440.0 17 12930.0 18 13582.0 19 14508.0 20 15750.0 21 17187.0 22 18992.0 23 21570.0 24 24989.0 25 29652.0 26 34586.0 27 40753.0 28 48931.0 29 57966.0 30 69180.0 31 84092.0 32 102989.0 33 130946.0 34 173494.0 35 236079.0 36 333967.0 37 450307.0 38 397486.0 39 99293.0 40 613.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.806600637123633 26.444899988514848 28.38736391607377 22.361135458287745 2 22.47591758024199 26.670505345435753 28.293027206257953 22.56054986806431 3 22.726347030496985 26.84078582210375 27.732590760790508 22.700276386608756 4 22.405636493620932 26.842228963282384 28.195126485564913 22.557008057531775 5 22.64818657626627 26.82423006760382 28.06113966770901 22.466443688420902 6 22.677593357233107 26.71445865802567 28.06497977788928 22.542968206851942 7 22.633833955474138 26.49507445096499 28.13444287130168 22.73664872225919 8 22.457217716658462 26.75107057258299 27.901730637779 22.889981072979555 9 22.410088157644655 26.54988137202632 28.231492883644076 22.80853758668495 10-11 22.902747742522276 26.687076575322124 27.90065624330465 22.50951943885095 12-13 22.95819244349969 26.369815715436324 27.85453650936821 22.817455331695776 14-15 22.657931450085623 26.684990974805967 27.958417044251316 22.698660530857097 16-17 22.978799930936276 26.535846074040997 27.95658279655936 22.52877119846337 18-19 22.859467054874976 26.454109266332388 27.812466821753517 22.873956857039115 20-21 22.838112172011744 26.680874280189354 27.799076813681022 22.681936734117883 22-23 22.976955109107212 26.792505300162432 27.400205620987094 22.83033396974326 24-25 22.90501685017617 26.881949386933968 27.36594441189513 22.847089350994736 26-27 22.946180614592897 26.979592916274658 27.269130932544407 22.805095536588038 28-29 23.17574059444022 26.82629963664996 27.239238631095613 22.75872113781421 30-31 22.93874708889799 26.82823251336349 27.35026621187231 22.882754185866215 32-33 22.8817750648069 27.138004215801587 27.20693888145618 22.77328183793534 34-35 22.884727280970875 26.81410295162241 27.364263907990388 22.936905859416328 36-37 22.80304703337791 26.952809823382314 27.547586990694654 22.696556152545117 38-39 22.810035395371113 26.850699925279585 27.620102908239364 22.719161771109935 40-41 22.719348425207265 26.877157929657063 27.414141887393573 22.9893517577421 42-43 22.6315685068735 27.126770328849243 27.250911790793957 22.9907493734833 44-45 22.827431625355846 26.838658435486273 27.524010758270666 22.809899180887218 46-47 22.760730528561812 26.813328071135643 27.39147709120876 23.03446430909378 48-49 22.81903614712759 26.845650389854566 27.34589069563233 22.989422767385516 50-51 22.72879286518376 27.132314624506527 27.17584725187064 22.963045258439074 52-53 22.813566220381073 26.9455861865549 27.316574429776104 22.92427316328792 54-55 22.77283338913405 27.04916484086215 27.257340565509413 22.92066120449439 56-57 22.76625035966439 27.152408339188412 27.170620629121263 22.910720672025942 58-59 22.91687487920524 27.13335118057218 27.193467532730065 22.756306407492517 60-61 22.828692793700522 27.066515545497605 27.11886886598476 22.985922794817114 62-63 22.826375916113836 27.017679964917065 27.201294788705972 22.954649330263134 64-65 22.847880873115002 27.036936763153584 27.045948817200973 23.069233546530445 66-67 22.736996711261533 27.165372703225128 27.210281827901504 22.887348757611832 68-69 22.81897303284422 26.962961115463237 27.312969939373875 22.90509591231867 70-71 22.714921693310693 26.88019686055049 27.36911636715173 23.035765078987083 72-73 22.842265870815133 26.660572648671167 27.389019501991708 23.10814197852199 74-75 22.648102725515496 26.75466579707268 27.241695141143612 23.355536336268212 76-77 22.59346125207066 26.624578235087398 27.59687053223145 23.18508998061049 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5159.0 1 3116.0 2 1048.0 3 1045.5 4 1068.0 5 1135.5 6 1224.0 7 1306.5 8 1407.5 9 1447.0 10 1541.0 11 1669.0 12 1831.0 13 1959.0 14 2044.5 15 2302.0 16 2706.0 17 2938.0 18 3221.5 19 3888.5 20 4934.5 21 6542.0 22 7487.0 23 8946.0 24 11923.5 25 16102.0 26 18762.0 27 21310.0 28 28318.5 29 35320.0 30 37861.0 31 43618.5 32 54646.5 33 65414.0 34 75722.0 35 80533.0 36 85620.5 37 95091.5 38 101135.5 39 102796.0 40 106215.0 41 111673.0 42 117037.5 43 120363.0 44 121075.0 45 122296.5 46 123706.0 47 125650.0 48 126694.0 49 126968.5 50 125158.5 51 119654.0 52 116234.0 53 114285.0 54 108103.5 55 97544.0 56 86182.0 57 81147.0 58 77275.5 59 66073.0 60 53687.0 61 48632.0 62 42595.5 63 31628.5 64 23051.0 65 19404.0 66 16608.0 67 12421.5 68 9286.0 69 6398.0 70 5255.0 71 4432.0 72 3077.5 73 2321.5 74 2097.0 75 1842.5 76 1266.5 77 782.5 78 620.0 79 520.5 80 340.5 81 226.0 82 160.5 83 129.0 84 113.5 85 84.0 86 68.5 87 67.0 88 58.0 89 51.0 90 51.5 91 50.0 92 41.5 93 31.5 94 28.5 95 30.0 96 33.0 97 29.5 98 29.0 99 55.5 100 79.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0026067590617934126 2 0.008349184821106437 3 0.011711526219651562 4 0.018776221068280086 5 0.013940494113069118 6 0.013487144711018091 7 0.017302835511614247 8 0.018285092549391473 9 0.017227277277939074 10-11 0.020325164858621098 12-13 0.02045739176755265 14-15 0.007839166743799031 16-17 0.005062401656236482 18-19 0.01042703624717365 20-21 0.00989812861144745 22-23 0.013147132659479819 24-25 0.02091074116960368 26-27 0.012656004140591204 28-29 0.007782498068542652 30-31 0.0075936024843547235 32-33 0.009671453910421936 34-35 0.010767048298711921 36-37 0.009803680819353486 38-39 0.016339468032255812 40-41 0.012504887673240862 42-43 0.014639407774564454 44-45 0.01673614875905046 46-47 0.015697223046016853 48-49 0.012731562374266377 50-51 0.025463124748532753 52-53 0.0298266127432739 54-55 0.0242919721265676 56-57 0.015130536293453067 58-59 0.01626390979858064 60-61 0.019777367664476107 62-63 0.02510422313857569 64-65 0.019248460028749907 66-67 0.019248460028749907 68-69 0.02818322116083892 70-71 0.05430748045402942 72-73 0.06603789623209978 74-75 0.07754163730914462 76-77 0.07474598266316329 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2646965.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.10756723912858 #Duplication Level Percentage of deduplicated Percentage of total 1 77.55860421823859 45.06741809584259 2 12.497066866655347 14.523483064921228 3 4.221548612995087 7.359117596485859 4 1.9356259236160251 4.498980540252742 5 1.0582135957721792 3.0745108834845967 6 0.6277134245451914 2.188494001419803 7 0.40564081395577484 1.649956061030902 8 0.28487626305476305 1.3242773288229057 9 0.2152623551287003 1.1257534597224714 >10 1.0805087049787736 11.463768690559231 >50 0.08022535883501683 3.2060380176838423 >100 0.03275473211255629 3.315745279099912 >500 0.001240782404283944 0.49941732904290315 >1k 6.530433706698746E-4 0.497595884484023 >5k 6.530433706698746E-5 0.20544376714683904 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5414 0.2045361385586889 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.7779116837585687E-5 3.7779116837585687E-5 2 0.0 0.0 0.0 1.1333735051275706E-4 3.7779116837585687E-5 3 0.0 0.0 0.0 1.5111646735034275E-4 3.7779116837585687E-5 4 0.0 0.0 0.0 6.800241030765424E-4 3.7779116837585687E-5 5 0.0 0.0 0.0 0.0012844899724779133 3.7779116837585687E-5 6 0.0 0.0 0.0 0.0024934217112806555 3.7779116837585687E-5 7 0.0 0.0 0.0 0.0055913092919626815 1.1333735051275706E-4 8 0.0 0.0 0.0 0.008386963937944024 1.5111646735034275E-4 9 0.0 0.0 0.0 0.012013759154352249 1.5111646735034275E-4 10 0.0 0.0 0.0 0.015036088501359103 1.8889558418792843E-4 11 0.0 0.0 0.0 0.01998515280708283 1.8889558418792843E-4 12 0.0 0.0 0.0 0.025538682982207926 1.8889558418792843E-4 13 0.0 0.0 0.0 0.033736751335964016 1.8889558418792843E-4 14 0.0 0.0 0.0 0.04280373937698458 1.8889558418792843E-4 15 0.0 0.0 0.0 0.05579975556911406 1.8889558418792843E-4 16 0.0 0.0 0.0 0.0678135147234663 2.6445381786309983E-4 17 0.0 0.0 0.0 0.07929836624209236 2.6445381786309983E-4 18 0.0 0.0 0.0 0.09032986835866738 2.6445381786309983E-4 19 0.0 0.0 0.0 0.1037414548360103 3.400120515382712E-4 20 0.0 0.0 0.0 0.11775750718275459 3.400120515382712E-4 21 0.0 0.0 0.0 0.1329824912683016 4.155702852134426E-4 22 0.0 0.0 0.0 0.14843415005487418 4.5334940205102824E-4 23 0.0 0.0 0.0 0.1636591341404212 4.5334940205102824E-4 24 0.0 0.0 0.0 0.18122642346989853 5.289076357261997E-4 25 0.0 0.0 0.0 0.19792479311211142 5.289076357261997E-4 26 0.0 0.0 0.0 0.21518984950688808 5.666867525637853E-4 27 0.0 0.0 0.0 0.23249268501850232 5.666867525637853E-4 28 0.0 0.0 0.0 0.24934217112806553 5.666867525637853E-4 29 0.0 0.0 0.0 0.26645611105549183 6.800241030765424E-4 30 0.0 0.0 0.0 0.2866301594467626 6.800241030765424E-4 31 0.0 0.0 0.0 0.30952430425033955 6.800241030765424E-4 32 0.0 0.0 0.0 0.33185176230135266 6.800241030765424E-4 33 0.0 0.0 0.0 0.3568615376478344 7.178032199141281E-4 34 0.0 0.0 0.0 0.38270245356474303 7.178032199141281E-4 35 0.0 0.0 0.0 0.410696779141394 7.555823367517137E-4 36 0.0 0.0 0.0 0.43971114087265983 7.555823367517137E-4 37 0.0 0.0 0.0 0.4704633419784546 7.555823367517137E-4 38 0.0 0.0 0.0 0.5014799969021124 7.933614535892994E-4 39 0.0 0.0 0.0 0.5330255594614964 7.933614535892994E-4 40 0.0 0.0 0.0 0.5655155999418202 7.933614535892994E-4 41 0.0 0.0 0.0 0.599176793044109 8.311405704268852E-4 42 0.0 0.0 0.0 0.6391093195414371 8.311405704268852E-4 43 0.0 0.0 0.0 0.6827441994888486 8.311405704268852E-4 44 0.0 0.0 0.0 0.7289858384980534 8.311405704268852E-4 45 0.0 0.0 0.0 0.7770030959986248 8.311405704268852E-4 46 0.0 0.0 0.0 0.8291004981176555 8.689196872644708E-4 47 0.0 0.0 0.0 0.8811601211198485 8.689196872644708E-4 48 0.0 0.0 0.0 0.9333330814725544 0.0010578152714523993 49 0.0 0.0 0.0 0.9881505800038912 0.0010578152714523993 50 0.0 0.0 0.0 1.045612616713859 0.0010578152714523993 51 0.0 0.0 0.0 1.10662589040656 0.0011333735051275707 52 0.0 0.0 0.0 1.1733815898585738 0.0011333735051275707 53 0.0 0.0 0.0 1.2415351166335784 0.0011333735051275707 54 0.0 0.0 0.0 1.3211734949272091 0.0011333735051275707 55 0.0 0.0 0.0 1.4012652226228908 0.0011333735051275707 56 0.0 0.0 0.0 1.4880816331156625 0.0011333735051275707 57 0.0 0.0 0.0 1.5750113809589472 0.0013600482061530848 58 0.0 0.0 0.0 1.6674946589773572 0.0013978273229906704 59 0.0 0.0 0.0 1.7585423305559387 0.0014356064398282561 60 0.0 0.0 0.0 1.8588458857597285 0.0014356064398282561 61 0.0 0.0 0.0 1.9631918064651404 0.0014356064398282561 62 0.0 0.0 0.0 2.0719578838405495 0.0014356064398282561 63 0.0 0.0 0.0 2.183595174095615 0.0014356064398282561 64 0.0 0.0 0.0 2.3041105568075135 0.001473385556665842 65 0.0 0.0 0.0 2.433692927560432 0.001473385556665842 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 190 0.0 28.029165 59 CGGTTAC 285 0.0 23.66147 4 CAATTCG 235 1.4551915E-11 21.14749 54 AATTCGC 220 1.2914825E-10 20.976234 55 TCGCGGA 240 1.8189894E-11 20.710049 58 CTCGCGC 260 1.8189894E-12 20.47666 36 ATTCGCG 230 2.3646862E-10 20.064981 56 TCGCGCT 250 3.45608E-11 19.877888 37 CGCGCTT 290 0.0 19.585608 38 CCTCGCG 305 1.8189894E-12 18.618158 35 GACGCGA 395 0.0 17.085436 69 CCCGTAT 240 1.6748527E-7 16.267262 2 CGATTCG 200 8.720213E-6 15.974511 15 TTAGTCG 200 8.720213E-6 15.974511 15 ATCGTAC 260 4.1573003E-7 15.015367 33 GCGGTTA 430 1.8189894E-12 14.8572035 3 CGATCGT 340 2.6793714E-9 14.6141615 1 ACGCGAC 475 0.0 14.2081585 70 TAGTCGG 225 2.6578982E-5 14.199029 16 TCGAACT 855 0.0 14.115993 24 >>END_MODULE