FastQCFastQC Report
Thu 26 May 2016
SRR936032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2646965
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62530.2362328175854233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45760.1728772386487921No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32770.12380216587676829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA9950.042.47709100-101
GGTATCA64450.039.3401871
ACGTATC4100.031.215773100-101
CGTACAT16250.031.137594100-101
GTATCAA128900.030.2900961
TACGCAG9150.029.275944100-101
CGGTATC9200.028.146275100-101
GCGTACA6300.027.401663100-101
GCGTATC3300.027.058071100-101
CGAGTAC11300.026.866508100-101
TCGAGTA4400.026.381617100-101
CCTGTCG5900.026.23132996-97
TCGTACA6950.026.12369100-101
CCGTACA7250.024.632174100-101
AGGTATC19200.024.493189100-101
TCGTATC6050.024.106277100-101
ACCGTAC2600.024.03914598-99
AGTATCA31250.023.430122100-101
ATCAACG165200.023.3012853
TATCAAC167700.023.2740942
Produced by FastQC (version 0.11.5)