FastQCFastQC Report
Thu 26 May 2016
SRR936031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404771
Sequences flagged as poor quality0
Sequence length77
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50450.2097912857398896No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT3150.028.1680741
ATTCGCG3200.027.73371556
CTCGCGC3300.026.88994636
CGGTTAC2650.026.784024
TCGCGCT3450.026.75187537
CGCGGAA3450.023.66561159
CAATTCG3500.023.33044254
CCTCGCG4000.022.1832835
CGCTCCG4100.021.64177725
TCGCGGA4150.020.52920558
AATTCGC4100.019.91374855
GACGCTC4500.019.72380623
ACGCTCC4700.018.88292324
CGCGCTT4700.018.8809638
ACTCGAG4900.018.83393714
AACTCGA4750.018.68065813
ATCGTTC5050.018.2714733
GCCCTCG5200.017.74662633
CGATTGC2055.7139914E-717.32927968
CGAACTC5750.017.2833125
Produced by FastQC (version 0.11.5)