##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936031_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2404771 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.107842285190564 39.0 36.0 41.0 28.0 41.0 2 35.96916795819644 39.0 36.0 41.0 27.0 41.0 3 35.878073213624084 39.0 35.0 41.0 27.0 41.0 4 35.75050347829377 39.0 35.0 41.0 27.0 41.0 5 35.67863218576738 39.0 35.0 41.0 27.0 41.0 6 35.5716045311591 39.0 35.0 40.0 27.0 41.0 7 35.408018892443394 39.0 35.0 40.0 27.0 41.0 8 35.227475713903736 38.0 34.0 40.0 26.0 41.0 9 35.13525279538052 38.0 34.0 40.0 25.0 41.0 10-11 35.34254176385194 39.0 35.0 40.0 26.0 41.0 12-13 35.518013565532854 39.0 35.0 41.0 27.0 41.0 14-15 35.46575786218314 39.0 35.0 41.0 26.0 41.0 16-17 35.30203707546373 39.0 35.0 41.0 25.0 41.0 18-19 35.14998268026352 39.0 35.0 41.0 25.0 41.0 20-21 34.94778255393133 39.0 34.5 41.0 24.0 41.0 22-23 34.6925293510276 39.0 34.0 41.0 23.5 41.0 24-25 34.465751832502974 39.0 34.0 40.5 22.5 41.0 26-27 33.54780850234804 37.5 33.0 39.5 21.0 40.5 28-29 33.57991842050657 38.0 33.0 39.5 20.5 40.5 30-31 33.94654043981734 38.0 33.0 40.0 20.5 41.0 32-33 33.78597047286415 38.0 33.0 40.0 19.0 41.0 34-35 33.51371897781535 38.0 33.0 40.0 17.5 41.0 36-37 33.22202197215452 37.5 32.5 40.0 15.0 41.0 38-39 32.9370430697975 37.0 32.0 40.0 13.0 41.0 40-41 32.5561878864973 36.5 32.0 40.0 9.5 41.0 42-43 32.199367424174696 36.0 31.0 39.0 8.0 41.0 44-45 31.825853064595343 35.5 31.0 39.0 7.0 40.5 46-47 31.432686937758316 35.0 31.0 38.5 2.0 40.0 48-49 30.973234457667694 35.0 30.5 37.5 2.0 40.0 50-51 30.526638919048843 35.0 30.0 37.0 2.0 39.0 52-53 30.068354325630175 35.0 30.0 36.5 2.0 39.0 54-55 29.608726361054753 34.0 29.0 36.0 2.0 38.5 56-57 29.154973800000082 34.0 29.0 35.5 2.0 37.0 58-59 28.724189954053838 34.0 29.0 35.0 2.0 37.0 60-61 28.37153038688507 34.0 28.5 35.0 2.0 36.5 62-63 28.02902147439403 34.0 27.5 35.0 2.0 36.0 64-65 27.653812982608322 34.0 27.0 35.0 2.0 36.0 66-67 27.267953372691203 33.5 26.0 35.0 2.0 35.0 68-69 26.902326666447657 33.0 25.5 35.0 2.0 35.0 70-71 26.48230663127591 33.0 25.0 35.0 2.0 35.0 72-73 25.998809450047425 33.0 24.0 35.0 2.0 35.0 74-75 25.41186333334858 33.0 21.5 35.0 2.0 35.0 76-77 23.743620078585444 30.5 9.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7520126339313791 1101 2 0.6199088752894752 1101 3 0.674011995306401 1101 4 0.738036305909155 1101 5 0.917696984147284 1101 6 0.7381239300485802 1101 7 0.7326721489914334 1101 8 0.9754910541130499 1101 9 1.0175744797309605 1101 10-11 0.9298946332012861 1101 12-13 0.9565700365376912 1101 14-15 0.9607795276512476 1101 16-17 0.8114039913119981 1101 18-19 0.9692401007185865 1101 20-21 0.8163044345995019 1101 22-23 0.8420992309848074 1101 24-25 0.9315599736483406 1101 26-27 0.7401977974672675 1101 28-29 0.858956969828192 1101 30-31 0.8711199514781782 1101 32-33 0.8379898641690957 1101 34-35 0.926568969749205 1101 36-37 0.7570942387729147 1101 38-39 0.8067750561889468 1101 40-41 0.7279287176488722 1101 42-43 0.8312945312765834 1101 44-45 0.6899656870590114 1101 46-47 0.6846640851096986 1101 48-49 0.816437890105437 1101 50-51 0.7934328121898986 1101 52-53 0.8295167225713556 1101 54-55 0.8226032286938754 1101 56-57 0.7525840929695597 1101 58-59 0.9306310465396557 1101 60-61 0.8062766620199184 1101 62-63 0.7111700259555747 1101 64-65 0.6823833597673108 1101 66-67 0.6659291844911657 1101 68-69 0.8254344589299514 1101 70-71 0.9292429915461184 1101 72-73 0.9373334861478604 1101 74-75 0.9790709018637678 1101 76-77 0.9573364533455013 1102 1 0.8799292006212269 1102 2 0.9740888586068834 1102 3 0.8452126839036396 1102 4 0.9538429632102918 1102 5 1.0261334354916158 1102 6 0.9888659765586425 1102 7 0.7013194851980984 1102 8 0.8740566330972115 1102 9 0.7802994569205879 1102 10-11 0.8020386397396635 1102 12-13 0.9036090723136638 1102 14-15 0.8361316645847054 1102 16-17 0.8130394684744218 1102 18-19 0.877417153818314 1102 20-21 0.8140963006815909 1102 22-23 0.8397654492073414 1102 24-25 0.8460888979030301 1102 26-27 0.8516946157581486 1102 28-29 0.9412002460041506 1102 30-31 0.9948477669002997 1102 32-33 0.9567587969725437 1102 34-35 0.9964211554152484 1102 36-37 1.003104919232399 1102 38-39 1.0140973515600535 1102 40-41 1.0647002095978095 1102 42-43 0.9203577266699696 1102 44-45 1.0574745205732476 1102 46-47 0.8569558746679284 1102 48-49 0.9018246134715149 1102 50-51 1.026679764250737 1102 52-53 1.1103214873260718 1102 54-55 1.1427802003769152 1102 56-57 1.213078703224383 1102 58-59 1.2469606885920008 1102 60-61 0.9941236370183439 1102 62-63 0.9122544530972334 1102 64-65 0.9022711260889089 1102 66-67 0.8974237207664082 1102 68-69 0.7957499563995789 1102 70-71 0.7407470316532283 1102 72-73 0.8245821463933076 1102 74-75 0.7450857196998122 1102 76-77 1.010421597580244 1103 1 0.6810431261866654 1103 2 0.4252517260159152 1103 3 0.5804173651372793 1103 4 0.5755182931458265 1103 5 0.6203031424849286 1103 6 0.560845198521406 1103 7 0.6391309332936643 1103 8 0.6292689777950784 1103 9 0.7158298929172418 1103 10-11 0.7170313368188062 1103 12-13 0.8069869033461643 1103 14-15 0.7019398225303206 1103 16-17 0.7338011470108938 1103 18-19 0.7356102715825159 1103 20-21 0.7650737183933174 1103 22-23 0.7405333682124322 1103 24-25 0.7664591567578256 1103 26-27 0.6434788106111213 1103 28-29 0.7818849562039958 1103 30-31 1.0021015924954995 1103 32-33 0.9069397590556534 1103 34-35 0.9297865093460942 1103 36-37 0.9555005380935739 1103 38-39 0.9568757639111922 1103 40-41 0.9132800523088562 1103 42-43 0.955578114553262 1103 44-45 0.9594494927270283 1103 46-47 0.7770383700575714 1103 48-49 0.913169743221097 1103 50-51 0.8913019646698892 1103 52-53 0.9218124453588246 1103 54-55 0.8969444762063965 1103 56-57 0.8887586343069032 1103 58-59 1.0234254343552323 1103 60-61 0.914310939294829 1103 62-63 0.711128503027119 1103 64-65 0.629879273755197 1103 66-67 0.8586561998932503 1103 68-69 0.8615459555956413 1103 70-71 0.7393416796555137 1103 72-73 0.8271029773613741 1103 74-75 0.7259748119590235 1103 76-77 0.9071997590852554 1104 1 0.7625808100466358 1104 2 0.8573882223150804 1104 3 0.7515981250664083 1104 4 0.6353617967856664 1104 5 0.8254474857125729 1104 6 0.5120834227916902 1104 7 0.6821295014503193 1104 8 0.7551421506318334 1104 9 0.7099845335230341 1104 10-11 0.6824013897924317 1104 12-13 0.7462460197204237 1104 14-15 0.6515635061951031 1104 16-17 0.628775190085129 1104 18-19 0.5358530673824404 1104 20-21 0.5046137520622196 1104 22-23 0.5770769761526751 1104 24-25 0.6718775363503156 1104 26-27 0.3989849840059847 1104 28-29 0.4347464318413117 1104 30-31 0.5509535255602529 1104 32-33 0.5512345723975685 1104 34-35 0.4952368271268739 1104 36-37 0.43037809185250353 1104 38-39 0.45592892128587437 1104 40-41 0.42874098692591645 1104 42-43 0.39715568493316766 1104 44-45 0.40706808107282555 1104 46-47 0.3074856266823325 1104 48-49 0.4539352495202955 1104 50-51 0.4012292784158902 1104 52-53 0.36879793098092506 1104 54-55 0.42915370831750366 1104 56-57 0.4784457407024689 1104 58-59 0.3662715737152169 1104 60-61 0.26184373521674686 1104 62-63 0.39982694751764924 1104 64-65 0.3616520528731577 1104 66-67 0.4420619322986532 1104 68-69 0.3825153508972434 1104 70-71 0.2767495400027542 1104 72-73 0.1660439508013667 1104 74-75 0.10087093088588617 1104 76-77 0.16669906374919563 1105 1 0.09081282576083538 1105 2 0.1397055682630537 1105 3 0.19263754718986803 1105 4 0.33062143023219903 1105 5 0.2062458310812545 1105 6 -0.026745632435343225 1105 7 -0.11469519867493005 1105 8 0.14490801752629068 1105 9 -0.12273761499384506 1105 10-11 0.08846646593549679 1105 12-13 0.1688798986190463 1105 14-15 -0.08894997900892321 1105 16-17 0.03245553033706727 1105 18-19 0.12369041769338907 1105 20-21 0.06024746922349067 1105 22-23 0.026531239056673428 1105 24-25 -0.07313264110911888 1105 26-27 -0.22148461283693877 1105 28-29 0.01952510934693663 1105 30-31 0.012688770680448158 1105 32-33 0.01015009620454066 1105 34-35 0.01663872835613489 1105 36-37 0.026568436675439955 1105 38-39 0.0952794342631833 1105 40-41 0.17676307766338795 1105 42-43 -0.04592963049498877 1105 44-45 -0.22257134955917834 1105 46-47 -0.28064905424995956 1105 48-49 -0.18622544957493048 1105 50-51 -0.09298940736589856 1105 52-53 -0.09902373661562791 1105 54-55 -0.17122269182110728 1105 56-57 -0.2226338140268851 1105 58-59 -0.09241455665933884 1105 60-61 -0.24556741559051076 1105 62-63 -0.2311804101267647 1105 64-65 -0.0398891561389334 1105 66-67 0.03104334487117555 1105 68-69 -0.27405953528219484 1105 70-71 -0.5206692591263753 1105 72-73 -0.5038403460749663 1105 74-75 -0.5238029940338649 1105 76-77 -0.4002996549085722 1106 1 0.22292059654240148 1106 2 0.3856576244652885 1106 3 0.12782691211204877 1106 4 0.19583601889833346 1106 5 0.06384677276768969 1106 6 0.30869484887105614 1106 7 0.372622116023976 1106 8 0.3834016929060482 1106 9 0.37210459647454996 1106 10-11 -0.0038275868662083212 1106 12-13 0.1040437595334609 1106 14-15 0.35675245554947566 1106 16-17 0.36954947477008915 1106 18-19 0.4095651210808384 1106 20-21 0.22987228938753645 1106 22-23 0.20834501308929276 1106 24-25 0.33850195092018254 1106 26-27 0.4294794947096321 1106 28-29 0.42480985738976784 1106 30-31 0.27625026990504864 1106 32-33 0.18532376635270253 1106 34-35 0.24791093926399554 1106 36-37 0.25543364846146943 1106 38-39 0.21439286637394872 1106 40-41 0.36431684160651656 1106 42-43 0.5419649342552972 1106 44-45 0.17407323854642343 1106 46-47 0.2986285190390312 1106 48-49 0.12779614942359174 1106 50-51 0.02614196162944893 1106 52-53 0.19805180382644494 1106 54-55 0.24418527888198227 1106 56-57 0.13056997513998425 1106 58-59 0.016795379627350115 1106 60-61 0.07045984473721134 1106 62-63 0.040191668122890434 1106 64-65 0.1965279511647431 1106 66-67 0.07069899405560776 1106 68-69 -0.1377589243070645 1106 70-71 -0.2662831303171238 1106 72-73 -0.11282747262342241 1106 74-75 -0.35209983078953044 1106 76-77 -0.07446260029449192 1107 1 0.312012962801667 1107 2 0.4286878060648931 1107 3 0.36208730809754996 1107 4 0.28761667863130924 1107 5 0.4017193069656102 1107 6 0.16157712788237433 1107 7 0.15064965650593365 1107 8 0.2729297525042398 1107 9 0.22769101566545658 1107 10-11 0.35923993467255855 1107 12-13 0.4820094614332433 1107 14-15 0.38603898949239124 1107 16-17 0.3488302711125968 1107 18-19 0.37345831064023116 1107 20-21 0.4416773566904979 1107 22-23 0.3990381698623935 1107 24-25 0.14654378986664796 1107 26-27 0.18086380464934138 1107 28-29 0.4063612863254846 1107 30-31 0.5816349623514512 1107 32-33 0.61916704789585 1107 34-35 0.6907986826566557 1107 36-37 0.6396671779053733 1107 38-39 0.4495494208993662 1107 40-41 0.4931074829427402 1107 42-43 0.5294951249622102 1107 44-45 0.6098938064777961 1107 46-47 0.53763664275262 1107 48-49 0.5823825868291017 1107 50-51 0.5011527993191116 1107 52-53 0.5260298005842472 1107 54-55 0.5717011711243813 1107 56-57 0.5905266243813472 1107 58-59 0.5247638137178043 1107 60-61 0.567505526965224 1107 62-63 0.6840960596640322 1107 64-65 0.723263711897701 1107 66-67 0.7843524794201748 1107 68-69 0.6803310011952028 1107 70-71 0.7610296690854348 1107 72-73 0.7029700538332904 1107 74-75 0.5124887012954922 1107 76-77 0.4326843551343664 1108 1 0.11915667792247575 1108 2 0.31707458044737535 1108 3 0.09425726954400204 1108 4 0.23588476779653433 1108 5 0.15717375015611879 1108 6 0.1049422630208312 1108 7 0.02703721918815205 1108 8 0.24332829973089787 1108 9 0.17772151288188098 1108 10-11 0.08422749399220208 1108 12-13 0.14091564527036127 1108 14-15 0.36903429973288127 1108 16-17 0.23778435091910666 1108 18-19 0.27461881682295797 1108 20-21 0.1914885582080288 1108 22-23 0.09180267709965761 1108 24-25 0.18681831507985436 1108 26-27 0.11596201885922852 1108 28-29 0.23459210631495608 1108 30-31 0.20315705337719692 1108 32-33 0.3588613893492365 1108 34-35 0.38179376202308646 1108 36-37 0.38039551279202755 1108 38-39 0.277101398330565 1108 40-41 0.23258481663844321 1108 42-43 0.20078461729751496 1108 44-45 0.10878632159943535 1108 46-47 0.21391939346115052 1108 48-49 0.1964808285982791 1108 50-51 0.22530855334393607 1108 52-53 0.1352560275317174 1108 54-55 0.22225636952615346 1108 56-57 0.22472694299449358 1108 58-59 0.17695125018193636 1108 60-61 0.1716678640209075 1108 62-63 0.13607130691334746 1108 64-65 0.04119021663937161 1108 66-67 -0.06681625121790447 1108 68-69 0.08617849362029872 1108 70-71 -0.0333407475573253 1108 72-73 0.12865226330505308 1108 74-75 0.07443548967600933 1108 76-77 0.030916417891901915 1109 1 0.10740111239248762 1109 2 0.09306178779296914 1109 3 -0.06653619566111502 1109 4 0.12330671346960287 1109 5 0.012315063268822257 1109 6 0.1573746602726871 1109 7 -0.03452468134459252 1109 8 0.2536344590776167 1109 9 0.031890466505672066 1109 10-11 -0.1222992318838152 1109 12-13 0.10166773687183905 1109 14-15 0.1725287465724179 1109 16-17 0.20190201581632294 1109 18-19 0.010625521013757577 1109 20-21 0.08178510923136884 1109 22-23 -0.04890064933453431 1109 24-25 -0.12333119915715685 1109 26-27 -0.2007642668215155 1109 28-29 0.04446629899322119 1109 30-31 0.0021690346975375974 1109 32-33 0.014017368544323006 1109 34-35 0.23398634134007779 1109 36-37 0.21462958949651778 1109 38-39 0.21682433173856452 1109 40-41 0.3316543805095833 1109 42-43 0.3590656070222167 1109 44-45 0.21877431385531665 1109 46-47 0.06672548320161198 1109 48-49 0.29691502976053386 1109 50-51 0.3213791073086476 1109 52-53 0.2702648528955649 1109 54-55 0.3333790767537259 1109 56-57 0.351627393280463 1109 58-59 0.19629974981688036 1109 60-61 0.08835050613604878 1109 62-63 0.2654233161222983 1109 64-65 0.21907552007210285 1109 66-67 0.2046673870558493 1109 68-69 0.13276130529095198 1109 70-71 0.1954008369577025 1109 72-73 0.138386675981657 1109 74-75 0.15234909669700514 1109 76-77 0.22815438846787472 1110 1 -0.495454828635161 1110 2 -0.5628205482845772 1110 3 -0.6416637560115319 1110 4 -0.5001536534726156 1110 5 -0.4516157675470751 1110 6 -0.6187352281455603 1110 7 -0.575086066771533 1110 8 -0.503096958862514 1110 9 -0.5876326305746744 1110 10-11 -0.6038245907963429 1110 12-13 -0.6594430489471819 1110 14-15 -0.7059489420290035 1110 16-17 -0.7013904980890473 1110 18-19 -0.665544587947899 1110 20-21 -0.7660176314809704 1110 22-23 -0.8687236850302327 1110 24-25 -0.6507473573401583 1110 26-27 -0.8087917729803991 1110 28-29 -0.677252936709742 1110 30-31 -0.6918076599869138 1110 32-33 -0.5869902570166445 1110 34-35 -0.3822210640492969 1110 36-37 -0.47623295945873423 1110 38-39 -0.4207998514138964 1110 40-41 -0.3873770834212493 1110 42-43 -0.3437579997782976 1110 44-45 -0.37117825508252267 1110 46-47 -0.6390147195362488 1110 48-49 -0.4104962669948726 1110 50-51 -0.32173928826039955 1110 52-53 -0.3737812622104606 1110 54-55 -0.3609247826255704 1110 56-57 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-0.9295652007147908 2314 36-37 -0.9206550754692486 2314 38-39 -0.6929701594302244 2314 40-41 -0.6972543849753166 2314 42-43 -0.6278989752533661 2314 44-45 -0.7140267968017575 2314 46-47 -0.684664163887799 2314 48-49 -0.7176439354120383 2314 50-51 -0.6028682893426236 2314 52-53 -0.4515487617444265 2314 54-55 -0.6373806454729127 2314 56-57 -0.5054074020250923 2314 58-59 -0.5810218702842782 2314 60-61 -0.41103715066752144 2314 62-63 -0.3691915061436326 2314 64-65 -0.1906123811081457 2314 66-67 -0.29112143719218153 2314 68-69 -0.08908624844987045 2314 70-71 -0.02167372770135856 2314 72-73 -0.060017292347705364 2314 74-75 -0.029733270167405124 2314 76-77 0.08827158139515134 2315 1 -1.4801736193658073 2315 2 -1.4267535846518768 2315 3 -1.331885054166932 2315 4 -1.3116692500111995 2315 5 -1.2868565290822787 2315 6 -1.1492405191720252 2315 7 -1.2557293007272534 2315 8 -1.3214160418531407 2315 9 -1.2707765213148505 2315 10-11 -1.1263808613959014 2315 12-13 -1.152013925268669 2315 14-15 -1.4476765507732168 2315 16-17 -1.4172786416923273 2315 18-19 -1.1949466266359607 2315 20-21 -1.2597842557689702 2315 22-23 -1.2853795205146454 2315 24-25 -1.4293182816924102 2315 26-27 -1.3084995857680823 2315 28-29 -1.4090200544047846 2315 30-31 -1.555263663433486 2315 32-33 -1.4630854132585611 2315 34-35 -1.4274087079142035 2315 36-37 -1.7099980118304714 2315 38-39 -1.5924270540069756 2315 40-41 -1.4473227649815747 2315 42-43 -1.6552604970147087 2315 44-45 -1.6553050301289325 2315 46-47 -1.5221477669651868 2315 48-49 -1.4533489602477836 2315 50-51 -1.4037877862434698 2315 52-53 -1.4612004013097675 2315 54-55 -1.476784981050887 2315 56-57 -1.5503932595801793 2315 58-59 -1.3769580354066058 2315 60-61 -1.3674720106995188 2315 62-63 -1.520705607655735 2315 64-65 -1.439626915746505 2315 66-67 -1.3860889225785336 2315 68-69 -1.5351748168993673 2315 70-71 -1.7195966889776493 2315 72-73 -2.9187676802574885 2315 74-75 -3.564438836331643 2315 76-77 -3.318914633703283 2316 1 -3.533526367702571 2316 2 -3.464452080472313 2316 3 -2.954035263380746 2316 4 -2.97241888271828 2316 5 -3.5640456637350937 2316 6 -3.193957553228252 2316 7 -3.5583991052991557 2316 8 -3.405164770733343 2316 9 -2.9048599995479876 2316 10-11 -3.108105728376799 2316 12-13 -3.213901423103316 2316 14-15 -3.147590806812083 2316 16-17 -4.285281140806255 2316 18-19 -4.076555887853527 2316 20-21 -3.40516114867744 2316 22-23 -3.4829822411277647 2316 24-25 -3.5195722447232143 2316 26-27 -3.261042876321241 2316 28-29 -3.5108766322277596 2316 30-31 -3.63852786282915 2316 32-33 -3.747799577102736 2316 34-35 -3.896441801604162 2316 36-37 -3.750312169586312 2316 38-39 -3.7185654808553466 2316 40-41 -3.7566098579423794 2316 42-43 -4.085757152420381 2316 44-45 -3.929076872913793 2316 46-47 -3.6273911225329982 2316 48-49 -3.8331093990133347 2316 50-51 -4.052600380965906 2316 52-53 -5.204289387305884 2316 54-55 -4.494235145544483 2316 56-57 -4.267129205144723 2316 58-59 -4.129450517407985 2316 60-61 -3.9638706918515787 2316 62-63 -4.082043416393791 2316 64-65 -4.046705974867798 2316 66-67 -4.079121265374628 2316 68-69 -4.101945879073522 2316 70-71 -3.8947958824190714 2316 72-73 -4.050715169872902 2316 74-75 -3.957969044951124 2316 76-77 -3.901275803503143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79376.0 3 6199.0 4 7627.0 5 8982.0 6 10082.0 7 10748.0 8 12066.0 9 12251.0 10 14638.0 11 15988.0 12 13626.0 13 11912.0 14 12049.0 15 12612.0 16 12679.0 17 13537.0 18 13935.0 19 14906.0 20 15685.0 21 17177.0 22 18467.0 23 21148.0 24 24591.0 25 28542.0 26 33049.0 27 39118.0 28 45722.0 29 54010.0 30 63930.0 31 76282.0 32 92568.0 33 116577.0 34 153159.0 35 203845.0 36 288971.0 37 389413.0 38 348871.0 39 89783.0 40 650.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.76972099756668 26.75619486710714 28.367070335129803 22.10701380019638 2 22.417203449606728 26.85343995794461 28.281210489892068 22.448146102556596 3 22.67024172840677 27.019165425210257 27.89687718606541 22.413715660317564 4 22.252902188994142 26.881390388921606 28.37149682747239 22.49421059461186 5 22.612557684565658 26.915360480707484 28.14062723543288 22.331454599293977 6 22.693705222296202 26.84102462157681 28.191940418803448 22.273329737323547 7 22.578475727985747 26.532644255843724 28.233669137996003 22.65521087817453 8 22.495376075709665 26.910054014755797 28.078772454678884 22.51579745485565 9 22.416236957970206 26.726189535432148 28.334779328398373 22.522794178199273 10-11 22.97088801262912 26.811240516669304 27.89638663810377 22.321484832597807 12-13 22.937349973350546 26.56583475591991 27.90146835057663 22.595346920152913 14-15 22.60473778099943 26.850870500666545 28.031791203770993 22.512600514563033 16-17 22.86398509856419 26.667402755676044 28.0529551582477 22.41565698751206 18-19 22.77079640349469 26.640371096528188 27.86863222830106 22.720200271676063 20-21 22.729042353275883 26.911733021213657 27.795933459944784 22.56329116556568 22-23 22.87161056303553 26.93835262331077 27.4452006450188 22.7448361686349 24-25 22.88575992400313 27.049242204466605 27.34535632787709 22.719641543653175 26-27 22.937695426436473 27.004353843642214 27.31445731891827 22.743493411003048 28-29 23.058747594923705 26.94924766572699 27.35406361981735 22.637941119531956 30-31 22.824033554768018 26.89419813673805 27.431430417336493 22.850337891157437 32-33 22.819536075089037 27.260095682875335 27.289457229355474 22.630911012680155 34-35 22.903981459435236 26.892594275423875 27.39555596220769 22.807868302933198 36-37 22.968280175837705 27.000008317772167 27.53904154311309 22.492669963277038 38-39 22.85745597025152 27.025811363254963 27.591871738176128 22.524860928317388 40-41 22.715693976626163 26.946312367357137 27.511548220553024 22.826445435463675 42-43 22.705653263039956 27.2444174199041 27.20078905373027 22.84914026332568 44-45 22.739071015524665 26.970548553670888 27.552765060521573 22.737615370282878 46-47 22.687313813392873 26.969844944667066 27.501607620746256 22.84123362119381 48-49 22.831722472455507 26.967982294325644 27.36437178089519 22.835923452323662 50-51 22.67038171219199 27.208389136320555 27.30053036323819 22.82069878824927 52-53 22.758363056535234 27.111137735112816 27.319116948423485 22.811382259928465 54-55 22.79784416133267 27.01561847462393 27.356269102973453 22.83026826106995 56-57 22.710252716485417 27.385525373655174 27.211750165487818 22.692471744371595 58-59 22.87954073681579 27.34270015509241 27.135307208989616 22.642451899102177 60-61 22.792848905946663 27.21664470899939 27.124952817877528 22.865553567176423 62-63 22.851397462275308 27.075156972607704 27.313140153022164 22.76030541209482 64-65 22.881814712406058 27.147894610414795 27.1572951101803 22.81299556699884 66-67 22.839092142400926 27.3141357086094 27.259250293093114 22.587521855896565 68-69 22.884520300871365 27.052463617807415 27.37839165154967 22.684624429771546 70-71 22.759301170035297 26.99993280561121 27.389993112055066 22.850772912298424 72-73 22.89252325363137 26.93121985996838 27.297696840652534 22.878560045747715 74-75 22.7458519180418 26.980709915400645 27.213340452991737 23.06009771356582 76-77 22.62735671283757 26.886270324850063 27.545734749586025 22.940638212726338 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5161.0 1 3080.5 2 999.0 3 1002.0 4 1006.0 5 1052.0 6 1140.0 7 1264.5 8 1349.5 9 1352.0 10 1415.0 11 1583.5 12 1706.0 13 1723.0 14 1869.0 15 2143.0 16 2423.5 17 2576.0 18 2723.5 19 3297.0 20 4261.5 21 5677.5 22 6555.0 23 7703.5 24 10414.5 25 14406.5 26 16836.0 27 19554.0 28 26110.0 29 32588.0 30 35228.0 31 40206.5 32 50396.5 33 60038.0 34 68719.5 35 72971.0 36 78412.0 37 87881.5 38 93293.0 39 94676.0 40 97366.5 41 102986.5 42 109821.0 43 113726.0 44 113448.0 45 113568.0 46 114902.0 47 116617.0 48 117396.0 49 117242.0 50 115142.5 51 109392.5 52 105588.0 53 103914.0 54 97427.5 55 86394.0 56 74713.0 57 69253.0 58 65556.0 59 55724.5 60 45392.0 61 41194.0 62 36288.0 63 26716.5 64 19430.5 65 16810.0 66 14463.0 67 11009.0 68 8308.0 69 5704.5 70 4695.0 71 4013.0 72 2821.0 73 2074.5 74 1838.0 75 1543.5 76 1032.5 77 663.0 78 510.0 79 411.0 80 268.5 81 195.0 82 141.5 83 118.0 84 106.5 85 90.5 86 76.5 87 67.0 88 62.5 89 54.5 90 45.5 91 40.0 92 39.0 93 33.5 94 29.0 95 31.5 96 34.0 97 34.5 98 38.5 99 84.5 100 127.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009980160273057185 2 0.013431632367489461 3 0.014013808383417798 4 0.015760336431202806 5 0.013098960358387556 6 0.010562336288985522 7 0.016508848451682094 8 0.017797952486951978 9 0.017839536488089718 10-11 0.02023061655350967 12-13 0.016903896462490606 14-15 0.008587096234942953 16-17 0.007090072193984375 18-19 0.018172208497191623 20-21 0.023494960642822124 22-23 0.020792000568869134 24-25 0.01919101652506621 26-27 0.0238484246524929 28-29 0.009210856252009028 30-31 0.009335608255422241 32-33 0.01104055230206951 34-35 0.013036584356680947 36-37 0.011685104319704454 38-39 0.014367272393088574 40-41 0.011976192327668621 42-43 0.015469248423238637 44-45 0.014096976385693275 46-47 0.024825648679229748 48-49 0.023702880648510817 50-51 0.03545036096992188 52-53 0.0384652010524079 54-55 0.0289008807907281 56-57 0.02143655258650408 58-59 0.01655043245281983 60-61 0.021769224595605985 62-63 0.025345448693451476 64-65 0.02675930473213458 66-67 0.02792365676399125 68-69 0.04239488915992416 70-71 0.05391365747507767 72-73 0.08433235430733321 74-75 0.08458185831415964 76-77 0.08896897043419104 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2404771.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.89245462188266 #Duplication Level Percentage of deduplicated Percentage of total 1 78.07963774749027 45.98301522937107 2 12.420881601334772 14.629924121407706 3 4.120498398585121 7.279987948746432 4 1.8155345920934831 4.276851543172948 5 0.9492299549495469 2.7951241023798956 6 0.605696375104847 2.1402567771300607 7 0.3912310619942832 1.6128390295618498 8 0.26249394752720523 1.2367130314611827 9 0.20028031239401073 1.0615499288388683 >10 1.0363819266131802 11.13241735993731 >50 0.07905167757395026 3.2098859352836357 >100 0.037734775260343294 3.8499970197950746 >500 0.0011348455402478887 0.47690121585279316 >1k 1.4185569253076925E-4 0.10380130694515903 >5k 7.092784626538463E-5 0.2107354501160271 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5045 0.2097912857398896 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.0792000568869135E-4 0.0 3 0.0 0.0 0.0 2.910880079641679E-4 0.0 4 0.0 0.0 0.0 5.821760159283358E-4 0.0 5 0.0 0.0 0.0 7.900960216170272E-4 0.0 6 0.0 0.0 0.0 0.0015386080420963161 0.0 7 0.0 0.0 0.0 0.003243552088743585 0.0 8 0.0 0.0 0.0 0.00457424012515121 0.0 9 0.0 0.0 0.0 0.005904928161558834 0.0 10 0.0 0.0 0.0 0.007277200199104198 0.0 11 0.0 0.0 0.0 0.010063328275332661 0.0 12 0.0 0.0 0.0 0.013639552373178153 0.0 13 0.0 0.0 0.0 0.018213792498329363 0.0 14 0.0 0.0 0.0 0.023744464649648554 0.0 15 0.0 0.0 0.0 0.029982064820309292 0.0 16 0.0 0.0 0.0 0.03551273697162848 0.0 17 0.0 0.0 0.0 0.04112657712522315 0.0 18 0.0 0.0 0.0 0.047239425292470674 0.0 19 0.0 0.0 0.0 0.05351860946426915 4.1584001137738274E-5 20 0.0 0.0 0.0 0.0606294736588224 4.1584001137738274E-5 21 0.0 0.0 0.0 0.06732449784199826 4.1584001137738274E-5 22 0.0 0.0 0.0 0.07555813006727044 4.1584001137738274E-5 23 0.0 0.0 0.0 0.08395809829709357 4.1584001137738274E-5 24 0.0 0.0 0.0 0.09260757053374313 4.1584001137738274E-5 25 0.0 0.0 0.0 0.10267089880907579 4.1584001137738274E-5 26 0.0 0.0 0.0 0.11202729906506691 4.1584001137738274E-5 27 0.0 0.0 0.0 0.12167478732902218 4.1584001137738274E-5 28 0.0 0.0 0.0 0.13098960358387554 8.316800227547655E-5 29 0.0 0.0 0.0 0.13988857982735153 8.316800227547655E-5 30 0.0 0.0 0.0 0.14957765209244456 1.247520034132148E-4 31 0.0 0.0 0.0 0.16084691640077164 1.247520034132148E-4 32 0.0 0.0 0.0 0.17348845274664407 1.247520034132148E-4 33 0.0 0.0 0.0 0.18750226113006185 1.247520034132148E-4 34 0.0 0.0 0.0 0.20072597349186264 1.247520034132148E-4 35 0.0 0.0 0.0 0.21540512589348423 1.663360045509531E-4 36 0.0 0.0 0.0 0.23033378230193227 1.663360045509531E-4 37 0.0 0.0 0.0 0.2467594627513389 1.663360045509531E-4 38 0.0 0.0 0.0 0.262311879176853 2.0792000568869135E-4 39 0.0 0.0 0.0 0.27807221560805584 2.0792000568869135E-4 40 0.0 0.0 0.0 0.2954543280836304 2.0792000568869135E-4 41 0.0 0.0 0.0 0.3127116885557918 2.0792000568869135E-4 42 0.0 0.0 0.0 0.33221458508939106 2.0792000568869135E-4 43 0.0 0.0 0.0 0.35375509767873947 2.0792000568869135E-4 44 0.0 0.0 0.0 0.37666788230563325 2.0792000568869135E-4 45 0.0 0.0 0.0 0.4002875949518686 2.0792000568869135E-4 46 0.0 0.0 0.0 0.4252795796356493 2.0792000568869135E-4 47 0.0 0.0 0.0 0.45185175636266406 2.0792000568869135E-4 48 0.0 0.0 0.0 0.47750908506464856 2.495040068264296E-4 49 0.0 0.0 0.0 0.5035406697768727 2.910880079641679E-4 50 0.0 0.0 0.0 0.5312771985357442 2.910880079641679E-4 51 0.0 0.0 0.0 0.5598454073173703 2.910880079641679E-4 52 0.0 0.0 0.0 0.5915740001854647 2.910880079641679E-4 53 0.0 0.0 0.0 0.624882785096793 2.910880079641679E-4 54 0.0 0.0 0.0 0.6627658101332725 2.910880079641679E-4 55 0.0 0.0 0.0 0.6989023071219671 2.910880079641679E-4 56 0.0 0.0 0.0 0.740070468248328 2.910880079641679E-4 57 0.0 0.0 0.0 0.7837752534440909 3.326720091019062E-4 58 0.0 0.0 0.0 0.8274384546387161 4.158400113773827E-4 59 0.0 0.0 0.0 0.8706442318208262 4.158400113773827E-4 60 0.0 0.0 0.0 0.9163450490712005 4.57424012515121E-4 61 0.0 0.0 0.0 0.966329018438762 4.57424012515121E-4 62 0.0 0.0 0.0 1.0184753558654858 4.57424012515121E-4 63 0.0 0.0 0.0 1.075653357429876 4.990080136528592E-4 64 0.0 0.0 0.0 1.135534319068219 4.990080136528592E-4 65 0.0 0.0 0.0 1.1957895367168017 4.990080136528592E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCGT 315 0.0 28.168074 1 ATTCGCG 320 0.0 27.733715 56 CTCGCGC 330 0.0 26.889946 36 CGGTTAC 265 0.0 26.78402 4 TCGCGCT 345 0.0 26.751875 37 CGCGGAA 345 0.0 23.665611 59 CAATTCG 350 0.0 23.330442 54 CCTCGCG 400 0.0 22.18328 35 CGCTCCG 410 0.0 21.641777 25 TCGCGGA 415 0.0 20.529205 58 AATTCGC 410 0.0 19.913748 55 GACGCTC 450 0.0 19.723806 23 ACGCTCC 470 0.0 18.882923 24 CGCGCTT 470 0.0 18.88096 38 ACTCGAG 490 0.0 18.833937 14 AACTCGA 475 0.0 18.680658 13 ATCGTTC 505 0.0 18.271473 3 GCCCTCG 520 0.0 17.746626 33 CGATTGC 205 5.7139914E-7 17.329279 68 CGAACTC 575 0.0 17.28331 25 >>END_MODULE