FastQCFastQC Report
Thu 26 May 2016
SRR936031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404771
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58020.24127037460115747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41180.1712429166852062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30020.12483517141549029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA8350.048.834217100-101
CGGTATC6850.039.10588100-101
GGTATCA60800.037.9719661
GCGTATC2950.034.30418100-101
CGTACAT14850.032.870594100-101
CGAGTAC9850.029.915007100-101
GTATCAA114150.029.8685151
ACGTATC4450.029.429495100-101
TACGCAG8100.029.02901100-101
CGTATAG3950.028.633646100-101
TCGGTAT3250.027.47439100-101
AGTATCA27500.025.759321100-101
TCGTACA8250.024.893461100-101
TCGTATC4100.024.682278100-101
AGCGTAC2100.024.09460398-99
CCGTATC4250.023.811138100-101
CCGAGTA5700.023.497831100-101
CCGGTAT2800.023.385939100-101
CCGTACA9200.022.969982100-101
TATCAAC146900.022.9646642
Produced by FastQC (version 0.11.5)