FastQCFastQC Report
Thu 26 May 2016
SRR936030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419127
Sequences flagged as poor quality0
Sequence length77
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50760.20982776017960197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG2700.024.98201256
CGCGGAA2750.023.2387859
TCGCGGA2750.023.238358
ACGCGAA1454.0233317E-722.03495254
CGATCGT3300.021.512051
CGGTTAC2008.9858077E-1021.298254
TAACGCG1355.3296244E-621.03835352
CCTCGCG3600.020.7049235
CTCGCGC3300.020.43729436
CCGTATA1106.782447E-419.37085766
CAATTCG3500.019.27183754
TCGCGCT3700.019.18721837
CGACACG950.00665154118.6815172
AACGCGA1803.1965465E-617.7511153
CGCTCCG4000.017.7474425
AACTCGA4300.017.33650413
AATTCGC3950.017.07701955
CGCGCTT4200.016.90372338
TTGCGTA4450.016.75108536
TGCGTAA3900.016.3829337
Produced by FastQC (version 0.11.5)