##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936030_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2419127 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.09218077430412 39.0 36.0 41.0 28.0 41.0 2 35.95949158518755 39.0 36.0 41.0 27.0 41.0 3 35.81867095030562 39.0 35.0 41.0 27.0 41.0 4 35.70623534853689 39.0 35.0 41.0 27.0 41.0 5 35.632826222021414 39.0 35.0 40.0 27.0 41.0 6 35.54083477221328 39.0 35.0 40.0 27.0 41.0 7 35.39387762610231 39.0 35.0 40.0 27.0 41.0 8 35.27116269629499 38.0 34.0 40.0 26.0 41.0 9 35.43584937872216 39.0 35.0 40.0 27.0 41.0 10-11 35.65045923591444 39.0 35.0 41.0 27.0 41.0 12-13 35.56330610174662 39.0 35.0 41.0 26.5 41.0 14-15 35.41512868071829 39.0 35.0 41.0 25.5 41.0 16-17 35.26022279938176 39.0 35.0 41.0 25.0 41.0 18-19 35.10349890683705 39.0 35.0 41.0 25.0 41.0 20-21 34.904779906139694 39.0 34.0 41.0 24.0 41.0 22-23 34.637301183443455 39.0 34.0 41.0 23.5 41.0 24-25 34.41722881849527 39.0 34.0 40.5 22.5 41.0 26-27 33.47895666494566 37.5 32.0 39.5 20.5 40.5 28-29 33.490078445654156 38.0 33.0 39.5 19.5 40.5 30-31 33.890824251889214 38.0 33.0 40.0 20.0 41.0 32-33 33.76555158121091 38.0 33.0 40.0 18.5 41.0 34-35 33.517509622272826 38.0 33.0 40.0 18.0 41.0 36-37 33.20742937431561 37.5 32.5 40.0 14.5 41.0 38-39 32.89349463670159 37.0 32.0 40.0 13.0 41.0 40-41 32.53538673248656 36.5 32.0 40.0 8.5 41.0 42-43 32.16618598362136 36.0 31.0 39.0 8.0 41.0 44-45 31.80176795182725 35.5 31.0 39.0 6.5 40.5 46-47 31.384098685186846 35.0 31.0 38.5 2.0 40.0 48-49 30.963356409150904 35.0 30.5 37.5 2.0 40.0 50-51 30.504889367114664 35.0 30.0 37.0 2.0 39.0 52-53 30.016713880668522 35.0 29.5 36.5 2.0 39.0 54-55 29.54557470525524 34.0 29.0 36.0 2.0 38.5 56-57 29.10392282009171 34.0 29.0 35.5 2.0 37.0 58-59 28.6803954484407 34.0 29.0 35.0 2.0 37.0 60-61 28.31835430715295 34.0 28.0 35.0 2.0 36.5 62-63 27.95058816672295 34.0 27.0 35.0 2.0 36.0 64-65 27.573954984587417 34.0 26.5 35.0 2.0 36.0 66-67 27.210373824937676 33.5 26.0 35.0 2.0 35.0 68-69 26.840380641446274 33.0 25.5 35.0 2.0 35.0 70-71 26.384723290674692 33.0 25.0 35.0 2.0 35.0 72-73 25.906919314281556 33.0 23.5 35.0 2.0 35.0 74-75 25.329477948036626 33.0 20.0 35.0 2.0 35.0 76-77 23.599062802407644 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.0461428646731505 1101 2 1.0163859778331883 1101 3 1.008964379129992 1101 4 0.9358174055994084 1101 5 1.090591596292029 1101 6 0.9343867267261601 1101 7 1.0044951613634154 1101 8 0.9752692284769537 1101 9 0.9482612513351043 1101 10-11 0.9531102096734472 1101 12-13 1.0117008393446199 1101 14-15 1.0560462453240689 1101 16-17 1.0434150823819124 1101 18-19 0.9791148256544062 1101 20-21 1.0800203351829225 1101 22-23 1.0234519742869637 1101 24-25 1.252439577812197 1101 26-27 1.2568252949836065 1101 28-29 1.3696278315606136 1101 30-31 1.2780178961578486 1101 32-33 1.3673872792515382 1101 34-35 1.5257529101868457 1101 36-37 1.4102474154898204 1101 38-39 1.2806165765548698 1101 40-41 1.3208572744207956 1101 42-43 1.3563767963236941 1101 44-45 1.1945046791613692 1101 46-47 1.3194426624156108 1101 48-49 1.3140190596568466 1101 50-51 1.2946196667436318 1101 52-53 1.3105366775134364 1101 54-55 1.3948448246281053 1101 56-57 1.2674803773168826 1101 58-59 1.2794782038016237 1101 60-61 1.1922493635532838 1101 62-63 1.0994855337739615 1101 64-65 1.0683596804254982 1101 66-67 1.3445854482597959 1101 68-69 1.4143740633327155 1101 70-71 1.3006916949469165 1101 72-73 1.2903319269922484 1101 74-75 1.3375916201323328 1101 76-77 1.6039885088218142 1102 1 1.1165514762724484 1102 2 1.1852551139694611 1102 3 1.3616349949816282 1102 4 1.14951276121041 1102 5 1.1131514178413084 1102 6 1.1854359850249736 1102 7 1.1581055009359673 1102 8 1.1232376923505143 1102 9 1.1790089746135877 1102 10-11 1.2784148479174533 1102 12-13 1.3526775252246424 1102 14-15 1.4129622862251665 1102 16-17 1.3579744246213963 1102 18-19 1.5051400416510674 1102 20-21 1.491993248034575 1102 22-23 1.252550917961912 1102 24-25 1.4780339526816206 1102 26-27 1.3285762044432161 1102 28-29 1.493921156864488 1102 30-31 1.5416866746552813 1102 32-33 1.5478716125807779 1102 34-35 1.4669618001545146 1102 36-37 1.4564001862691285 1102 38-39 1.4669348167505234 1102 40-41 1.473484030353795 1102 42-43 1.4572837979336768 1102 44-45 1.306900521456157 1102 46-47 1.449197738183429 1102 48-49 1.2884412762802917 1102 50-51 1.2148122816858056 1102 52-53 1.2750182087238748 1102 54-55 1.374224517869834 1102 56-57 1.2818765698765198 1102 58-59 1.4694542536744208 1102 60-61 1.492505840380499 1102 62-63 1.4234544032173453 1102 64-65 1.3168353370184676 1102 66-67 1.342844109633571 1102 68-69 1.4448520980396609 1102 70-71 1.2877545551973881 1102 72-73 1.1605691699777338 1102 74-75 1.123949649185878 1102 76-77 1.0880979297172608 1103 1 0.4765152050986856 1103 2 0.5513696112210766 1103 3 0.5212318988443201 1103 4 0.631881979323559 1103 5 0.4640858679777935 1103 6 0.34161853746860515 1103 7 0.38406033023709085 1103 8 0.47632859464270183 1103 9 0.4475068993041589 1103 10-11 0.5338411276915949 1103 12-13 0.572519913887092 1103 14-15 0.604020133138377 1103 16-17 0.5851491993287894 1103 18-19 0.6214740355934012 1103 20-21 0.5053989990722272 1103 22-23 0.4467991324479428 1103 24-25 0.39403271871385215 1103 26-27 0.3218637937189115 1103 28-29 0.4164973450258742 1103 30-31 0.5882012766071938 1103 32-33 0.5582913485864651 1103 34-35 0.6162894746861767 1103 36-37 0.4867825293121655 1103 38-39 0.30516482086375163 1103 40-41 0.49307476437764564 1103 42-43 0.39424244131699737 1103 44-45 0.4876109877463932 1103 46-47 0.5032015896585804 1103 48-49 0.48502374645940804 1103 50-51 0.5814203660443304 1103 52-53 0.6643339175074843 1103 54-55 0.6300140702760721 1103 56-57 0.6150521672290985 1103 58-59 0.3928859928211139 1103 60-61 0.3792425565305173 1103 62-63 0.3418296789904467 1103 64-65 0.338753409037313 1103 66-67 0.6180153694024817 1103 68-69 0.6492741898019894 1103 70-71 0.5852514702779708 1103 72-73 0.6223206563479984 1103 74-75 0.6998414713449463 1103 76-77 0.8462350123183171 1104 1 0.2770404907859074 1104 2 0.2711345675224308 1104 3 0.4467854601261436 1104 4 0.4163065010736702 1104 5 0.31004980870276455 1104 6 0.3308274521728194 1104 7 0.29350122206828644 1104 8 0.3608806496966821 1104 9 0.16629470156440362 1104 10-11 0.36718296925807437 1104 12-13 0.4566998314504289 1104 14-15 0.3998287610279476 1104 16-17 0.4608578028661796 1104 18-19 0.32300804297121744 1104 20-21 0.3509623012287477 1104 22-23 0.2749012853616435 1104 24-25 0.08055712050688868 1104 26-27 0.12583193745093268 1104 28-29 0.23062810724931637 1104 30-31 0.29755937100348007 1104 32-33 0.31017566668013785 1104 34-35 0.42210405533250395 1104 36-37 0.3994164437908907 1104 38-39 0.3712876821554971 1104 40-41 0.4319964134716585 1104 42-43 0.3659617958938526 1104 44-45 0.25901935587930325 1104 46-47 0.22102874324872346 1104 48-49 0.11230108178901688 1104 50-51 0.13298882725671035 1104 52-53 0.06760685140482892 1104 54-55 0.1235843714314484 1104 56-57 0.0732643559495294 1104 58-59 0.050660563726566465 1104 60-61 0.04420832817915965 1104 62-63 0.08994500012363105 1104 64-65 0.05120296844311767 1104 66-67 -0.10606831192660238 1104 68-69 -0.5241107641543401 1104 70-71 -0.388191658346674 1104 72-73 -0.5213240542668522 1104 74-75 -0.6290366143927884 1104 76-77 -0.4079743984489568 1105 1 0.3567497220564064 1105 2 0.7178721756908644 1105 3 0.5881829098630362 1105 4 0.6202590682699309 1105 5 0.4766591992822242 1105 6 0.46599021999351464 1105 7 0.5460768180323683 1105 8 0.5402641830385875 1105 9 0.543367764510613 1105 10-11 0.7808163959986913 1105 12-13 0.8574762776211031 1105 14-15 0.7952013109147629 1105 16-17 0.7035294211352934 1105 18-19 0.5410180701801792 1105 20-21 0.615643630692631 1105 22-23 0.6836434472019306 1105 24-25 0.5863058325082804 1105 26-27 0.4895888897186964 1105 28-29 0.6212105988143932 1105 30-31 0.8196389013621541 1105 32-33 0.814322193832119 1105 34-35 0.8105468525717612 1105 36-37 0.7969256562646123 1105 38-39 0.6900834112557135 1105 40-41 0.7733644196735199 1105 42-43 0.8950240451623586 1105 44-45 0.6090905928270018 1105 46-47 0.5734846211930531 1105 48-49 0.5804136085240508 1105 50-51 0.7400026125735728 1105 52-53 0.6033169016015059 1105 54-55 0.5014894683730837 1105 56-57 0.6357789907774674 1105 58-59 0.7435677926940087 1105 60-61 0.5855794374549781 1105 62-63 0.558242017088709 1105 64-65 0.4704343227583472 1105 66-67 0.38753989074621487 1105 68-69 0.3743287463232612 1105 70-71 0.3984374100017405 1105 72-73 -0.06429003655115295 1105 74-75 -0.1043109109714031 1105 76-77 -0.19416885810658968 1106 1 0.5536873373542193 1106 2 0.5631433818015239 1106 3 0.5117344806929793 1106 4 0.6613907522019815 1106 5 0.5099665352031835 1106 6 0.48971878348769593 1106 7 0.4012885151929808 1106 8 0.4958232191707168 1106 9 0.6193655599870667 1106 10-11 0.49122206076179253 1106 12-13 0.8700202518254017 1106 14-15 0.7840962819033308 1106 16-17 0.7941596258519965 1106 18-19 0.8217472699783954 1106 20-21 0.8451807359850108 1106 22-23 0.9430541451994898 1106 24-25 0.8303883920221296 1106 26-27 0.7516802256311053 1106 28-29 0.9694588561057387 1106 30-31 1.211130976427853 1106 32-33 1.1014296689880183 1106 34-35 1.019700578888326 1106 36-37 1.0324523230346259 1106 38-39 1.1577377479501862 1106 40-41 1.1740045903619531 1106 42-43 1.1740036315807671 1106 44-45 1.103888259367693 1106 46-47 1.2505260194440098 1106 48-49 1.2208098810760042 1106 50-51 1.1982564524484012 1106 52-53 1.1964544578483078 1106 54-55 1.124444666759711 1106 56-57 1.160308324465035 1106 58-59 1.162220659884298 1106 60-61 0.8081425898737642 1106 62-63 0.8973543845335321 1106 64-65 0.96887029063274 1106 66-67 0.7701049996529186 1106 68-69 0.7733192662661637 1106 70-71 0.5024769517274095 1106 72-73 0.6089583053787919 1106 74-75 0.7426172515318505 1106 76-77 0.5829059144784203 1107 1 0.07304548333108585 1107 2 0.2019424426449703 1107 3 0.26067543403253524 1107 4 0.2038950726132498 1107 5 0.15380763557190136 1107 6 0.06120937216582689 1107 7 0.060541508633164653 1107 8 0.01819438842338883 1107 9 -0.03142927090861036 1107 10-11 0.18731084945919463 1107 12-13 0.25277359637481567 1107 14-15 0.05546039500417521 1107 16-17 0.12783332371504486 1107 18-19 0.22419089320387542 1107 20-21 0.1853989990722269 1107 22-23 0.25787114554123747 1107 24-25 0.1583780542293951 1107 26-27 0.08621731254051923 1107 28-29 0.07212745959215283 1107 30-31 0.2937986579492531 1107 32-33 0.33683472010855553 1107 34-35 0.31585575946522937 1107 36-37 0.3560051152368757 1107 38-39 0.3238718585069549 1107 40-41 0.2846786923645581 1107 42-43 0.28880872609605035 1107 44-45 0.2578974034583439 1107 46-47 0.2360984800022763 1107 48-49 0.22044109506824228 1107 50-51 0.20822069337657467 1107 52-53 0.08689529230290205 1107 54-55 0.2604805187212449 1107 56-57 0.24806362385757907 1107 58-59 0.1557010501042555 1107 60-61 0.2634487758431199 1107 62-63 0.10210791139633457 1107 64-65 0.12261592949557709 1107 66-67 0.2715587409245792 1107 68-69 0.2137668248265392 1107 70-71 0.054490422814794215 1107 72-73 0.1818705745149387 1107 74-75 0.08625718329257381 1107 76-77 -0.003347639072845965 1108 1 0.48646544531830926 1108 2 0.6879430497934891 1108 3 0.67031391668921 1108 4 0.5834262483695412 1108 5 0.6278744747039369 1108 6 0.5322860048673732 1108 7 0.7142233364141717 1108 8 0.6553419783077672 1108 9 0.5575721017749871 1108 10-11 0.5856530700388873 1108 12-13 0.7975851163579293 1108 14-15 0.781064573769811 1108 16-17 0.7420066118202158 1108 18-19 0.7645042346325255 1108 20-21 0.5576793696967997 1108 22-23 0.6529410336147308 1108 24-25 0.7194779829554392 1108 26-27 0.6957845212412153 1108 28-29 0.8431093903244289 1108 30-31 0.9385976389956596 1108 32-33 0.7720173266235264 1108 34-35 0.8989130162098533 1108 36-37 0.7990783426271904 1108 38-39 0.9729693850367056 1108 40-41 0.8119637485917863 1108 42-43 0.9589455984892723 1108 44-45 0.9384534723037063 1108 46-47 0.7675632917862316 1108 48-49 0.8023015433022316 1108 50-51 0.8546058499152984 1108 52-53 0.9689804514814035 1108 54-55 0.9651710709624162 1108 56-57 1.0294756401186014 1108 58-59 1.0491685940565283 1108 60-61 1.133725226400113 1108 62-63 0.8589007153665591 1108 64-65 0.8715897165184394 1108 66-67 0.862493749635842 1108 68-69 0.928128856926218 1108 70-71 0.9426751147529195 1108 72-73 1.1449450557989245 1108 74-75 1.1859730773847552 1108 76-77 0.9925656574796058 1109 1 -0.22147100275262943 1109 2 -0.4536964509214627 1109 3 -0.31074529306036425 1109 4 -0.12285744943575594 1109 5 -0.27629847784692885 1109 6 -0.2648937102016973 1109 7 -0.24562445773241137 1109 8 -0.26621559820510754 1109 9 -0.3741628678559934 1109 10-11 -0.41674759112681414 1109 12-13 -0.31699857226451655 1109 14-15 -0.13848728641463026 1109 16-17 -0.1175154368751663 1109 18-19 -0.36724582112053383 1109 20-21 -0.24949063778066716 1109 22-23 -0.2649746382424354 1109 24-25 -0.40354958857245293 1109 26-27 -0.5535538211219233 1109 28-29 -0.26958746717075144 1109 30-31 -0.009627281966174905 1109 32-33 -0.026125686534200554 1109 34-35 -0.21219053362026585 1109 36-37 -0.15736047986818136 1109 38-39 -0.0016564955336804132 1109 40-41 0.024946914447305346 1109 42-43 -0.0480932253179418 1109 44-45 -0.037737839401813744 1109 46-47 -0.08004752561921435 1109 48-49 -0.02150788290039074 1109 50-51 -0.13751009349206456 1109 52-53 -0.05197023657694544 1109 54-55 0.10246109510020673 1109 56-57 -0.012119750087226322 1109 58-59 -0.0680180245531865 1109 60-61 -0.02962672954841139 1109 62-63 -0.13588752139373028 1109 64-65 -0.01259232050159298 1109 66-67 0.011971310669043334 1109 68-69 -0.14850478971744607 1109 70-71 -0.148082093789764 1109 72-73 -0.36014774024982543 1109 74-75 -0.4483782685597042 1109 76-77 -0.2874290787756202 1110 1 -0.6961274533480477 1110 2 -0.7191739569316908 1110 3 -0.9499966637533106 1110 4 -0.7668390859263425 1110 5 -0.7930837683904031 1110 6 -0.7777753259835976 1110 7 -0.7058450235792435 1110 8 -0.9043944921761664 1110 9 -1.097220063580835 1110 10-11 -0.9013715129619797 1110 12-13 -0.8342879284639437 1110 14-15 -0.7868504780938537 1110 16-17 -0.845185863197834 1110 18-19 -0.8747965873338615 1110 20-21 -1.0548586256677623 1110 22-23 -0.8816213706052949 1110 24-25 -0.9247222621146634 1110 26-27 -1.0119717574927733 1110 28-29 -0.8582358830522168 1110 30-31 -0.7477485684337069 1110 32-33 -0.5378157945690276 1110 34-35 -0.7261146887434791 1110 36-37 -0.6880649758879471 1110 38-39 -0.7820264513045956 1110 40-41 -0.6779985885030868 1110 42-43 -0.8109299350289731 1110 44-45 -0.7591843242184524 1110 46-47 -0.9423208856774465 1110 48-49 -0.8836581404496826 1110 50-51 -0.832223136115072 1110 52-53 -0.7845075229857095 1110 54-55 -0.8295256468713426 1110 56-57 -0.663674745534351 1110 58-59 -0.6935567976123522 1110 60-61 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-0.7479335832909157 2314 30-31 -0.7916457248474273 2314 32-33 -0.938686440245398 2314 34-35 -1.0687953528324599 2314 36-37 -1.4453696251033108 2314 38-39 -1.1081326369485964 2314 40-41 -0.9952218266941699 2314 42-43 -0.7349834463383544 2314 44-45 -0.6500732323001799 2314 46-47 -0.6813120181677128 2314 48-49 -0.5549281682834746 2314 50-51 -0.46850878733859247 2314 52-53 -0.3445312553507236 2314 54-55 -0.39918272504503705 2314 56-57 -0.5670091678527847 2314 58-59 -0.6006799284486988 2314 60-61 -0.6610627622415741 2314 62-63 -0.635487118440988 2314 64-65 -0.4464551739549769 2314 66-67 -0.5259465392355303 2314 68-69 -0.6353774935588525 2314 70-71 -0.5183678874296156 2314 72-73 -0.5367604479548014 2314 74-75 -0.5753177451216125 2314 76-77 -0.5579307687656332 2315 1 -1.2564077865569487 2315 2 -1.0598552556077578 2315 3 -0.9644702903972941 2315 4 -0.8633992966563682 2315 5 -1.1864455850505067 2315 6 -1.080589794581769 2315 7 -1.4154805756347528 2315 8 -1.3014276636323814 2315 9 -1.0248015035655627 2315 10-11 -1.2419343441808053 2315 12-13 -1.129578648172206 2315 14-15 -1.0801776068006532 2315 16-17 -0.933612231837877 2315 18-19 -0.9518757196179806 2315 20-21 -0.9864764820374816 2315 22-23 -1.179222205729836 2315 24-25 -1.3049510236268844 2315 26-27 -1.1237588007325456 2315 28-29 -1.2609319126026008 2315 30-31 -1.269329745346809 2315 32-33 -1.1768841602351685 2315 34-35 -1.2396571952648756 2315 36-37 -1.3684791943692076 2315 38-39 -1.2301983592329364 2315 40-41 -1.447900695178344 2315 42-43 -1.595209786413399 2315 44-45 -1.5161489038692082 2315 46-47 -1.2995134086479183 2315 48-49 -1.3442552817759612 2315 50-51 -1.3417344820019785 2315 52-53 -1.2179891110973742 2315 54-55 -1.4648529125117484 2315 56-57 -1.5407229967330878 2315 58-59 -1.3463506977599131 2315 60-61 -1.5823725864937792 2315 62-63 -1.4874083971865417 2315 64-65 -1.359057195390438 2315 66-67 -1.4985608229798189 2315 68-69 -1.5367586950087997 2315 70-71 -1.5477831388300167 2315 72-73 -1.48204335510626 2315 74-75 -1.452610263729106 2315 76-77 -1.4376940759595378 2316 1 -2.4035119389052255 2316 2 -2.0840276594824303 2316 3 -2.236788409504946 2316 4 -3.2171998416418788 2316 5 -2.820498820486833 2316 6 -2.70880773925127 2316 7 -2.29557123956355 2316 8 -2.240232889008823 2316 9 -2.5395662653722226 2316 10-11 -2.312798643239489 2316 12-13 -2.9232891512171406 2316 14-15 -4.137668174250912 2316 16-17 -3.2057645108758592 2316 18-19 -2.7158984579309333 2316 20-21 -2.4909004787367195 2316 22-23 -2.9019001070234083 2316 24-25 -2.8273491428020776 2316 26-27 -2.7323758845253323 2316 28-29 -3.2101668389575124 2316 30-31 -3.4467380217865156 2316 32-33 -3.4340973237572783 2316 34-35 -3.4351143171987637 2316 36-37 -3.246691158382827 2316 38-39 -3.3958409968907475 2316 40-41 -3.1285176286807683 2316 42-43 -3.408679180371724 2316 44-45 -3.2129911457061056 2316 46-47 -3.3615596175875453 2316 48-49 -3.113850935964024 2316 50-51 -3.33987484982498 2316 52-53 -3.1849504904139287 2316 54-55 -3.163151268155037 2316 56-57 -3.1242674120388365 2316 58-59 -3.2295031760592785 2316 60-61 -2.9529632248112883 2316 62-63 -2.94307055851959 2316 64-65 -2.9482408063106362 2316 66-67 -3.022486049864792 2316 68-69 -3.1913126337702593 2316 70-71 -3.1330106514231666 2316 72-73 -3.0054761277495885 2316 74-75 -3.138633499069744 2316 76-77 -3.4055814015284334 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 80290.0 3 6158.0 4 7665.0 5 9412.0 6 10429.0 7 10999.0 8 12504.0 9 12494.0 10 14534.0 11 16032.0 12 13411.0 13 12091.0 14 12257.0 15 12890.0 16 12998.0 17 13699.0 18 14494.0 19 15028.0 20 16122.0 21 17201.0 22 19063.0 23 21707.0 24 24554.0 25 29026.0 26 34045.0 27 39516.0 28 46122.0 29 53939.0 30 63906.0 31 76826.0 32 92825.0 33 116327.0 34 153062.0 35 203073.0 36 288296.0 37 393871.0 38 353799.0 39 87891.0 40 571.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.73466107657494 26.678371024562615 28.398928170738664 22.18803972812378 2 22.423564439362316 26.870855869096 28.256880202965522 22.448699488576164 3 22.768697645229068 27.029262859708435 27.804429737633917 22.39760975742858 4 22.17224484733219 26.98658342463771 28.336179273561697 22.5049924544684 5 22.624767754242438 26.943319218744705 28.090511970113784 22.341401056899073 6 22.669781713802355 26.830321023219394 28.1817366236191 22.318160639359153 7 22.57889268478028 26.495257570501803 28.33037788528286 22.595471859435058 8 22.464329569556824 26.834830712747088 28.110077025803438 22.590762691892653 9 22.431140695414893 26.71860917021541 28.28738578575268 22.562864348617026 10-11 22.928004501747175 26.813614312149113 27.94254483916735 22.315836346936358 12-13 22.91808060563274 26.574575869992383 27.920421809476654 22.586921714898224 14-15 22.613384419556354 26.831677151386344 28.062765278841727 22.49217315021557 16-17 22.84732463887043 26.660307217217227 28.04097607558581 22.45139206832653 18-19 22.77859336648698 26.610721093880162 27.911365741994405 22.699319797638452 20-21 22.711575995128282 26.91397059522692 27.81150120780087 22.562952201843927 22-23 22.8708286486282 26.97046501421897 27.444217125566222 22.714489211586606 24-25 22.876436781609193 27.04963615314645 27.348796824609277 22.725130240635078 26-27 22.913324349515573 27.020868048471275 27.323105506939722 22.742702095073433 28-29 23.095427769893202 26.96887754870027 27.31413365171115 22.62156102969537 30-31 22.822969563214173 26.90183661670452 27.429073539287906 22.8461202807934 32-33 22.84001043454247 27.229463609119726 27.32421785373368 22.60630810260413 34-35 22.923155186409442 26.91760628115239 27.362217379252296 22.797021153185877 36-37 22.922320949824485 26.96386646927577 27.59029041845244 22.523522162447303 38-39 22.84946169874975 26.997709532314612 27.622482139313355 22.53034662962228 40-41 22.784703107054767 26.92110361341779 27.491692223226387 22.802501056301054 42-43 22.69925663775354 27.255905967569895 27.205445810628675 22.839391584047895 44-45 22.783047487335644 26.96620805206108 27.538809627909238 22.71193483269404 46-47 22.6603674631835 26.985281668784523 27.49704027153222 22.857310596499758 48-49 22.81380901903261 26.96050018273554 27.425111501755417 22.80057929647643 50-51 22.66687506215168 27.198215737704512 27.30088359272162 22.834025607422188 52-53 22.796433273568116 27.11923243597293 27.29763905337188 22.786695237087073 54-55 22.817244595170948 27.022026178531107 27.3491079316033 22.811621294694643 56-57 22.661865818952393 27.386206741155878 27.21008194557537 22.741845494316358 58-59 22.85453226196555 27.295278691371244 27.184103956026235 22.66608509063697 60-61 22.777426571610846 27.199109916321053 27.172132045617204 22.8513314664509 62-63 22.85665849352282 27.049047132715042 27.36341684274072 22.730877531021424 64-65 22.878861330618278 27.136509052194178 27.177296900599003 22.80733271658854 66-67 22.80232626888615 27.267630487749916 27.298164213976715 22.631879029387214 68-69 22.87982302440134 27.045613599830464 27.40862032428297 22.665943051485222 70-71 22.761206144754496 27.016677975299135 27.34754078087262 22.874575099073756 72-73 22.90345211572079 26.93218210413018 27.284678139247827 22.879687640901206 74-75 22.76024012124795 26.949433754944526 27.229179435424232 23.061146688383293 76-77 22.631526582696885 26.837944721261707 27.58430676735649 22.946221928684913 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5146.0 1 3060.0 2 953.5 3 966.5 4 1000.0 5 1051.5 6 1127.0 7 1254.0 8 1378.0 9 1399.0 10 1443.5 11 1542.0 12 1655.5 13 1715.0 14 1777.0 15 2084.5 16 2429.0 17 2528.0 18 2801.5 19 3421.0 20 4261.5 21 5732.0 22 6708.0 23 7757.0 24 10360.5 25 14364.5 26 16814.0 27 19488.5 28 26147.5 29 32762.5 30 35393.0 31 40490.5 32 50615.0 33 60385.5 34 69496.0 35 73863.0 36 79034.0 37 87669.5 38 93310.5 39 95487.0 40 98366.0 41 103902.0 42 110649.0 43 114739.0 44 114915.5 45 115240.0 46 116114.5 47 117633.0 48 118425.0 49 117923.5 50 114986.0 51 109528.5 52 106507.0 53 104920.5 54 98414.0 55 86926.0 56 74738.0 57 69118.0 58 65661.0 59 56062.5 60 45551.0 61 41181.0 62 36355.5 63 26815.5 64 19426.0 65 16751.0 66 14551.0 67 11245.5 68 8402.5 69 5691.0 70 4717.0 71 4009.5 72 2808.5 73 2046.0 74 1777.0 75 1506.0 76 1036.0 77 672.5 78 508.0 79 409.0 80 269.5 81 181.0 82 128.5 83 124.0 84 100.5 85 76.0 86 66.5 87 58.0 88 53.0 89 47.0 90 43.5 91 41.0 92 36.0 93 26.5 94 23.0 95 22.5 96 21.0 97 23.5 98 32.5 99 59.5 100 80.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003513664226805786 2 0.008019421882356735 3 0.012111807275930532 4 0.019717856896310115 5 0.01459204084779344 6 0.013641284645245992 7 0.017775007265017503 8 0.018891112372355814 9 0.01765099558642436 10-11 0.02110265397393357 12-13 0.020647944485758706 14-15 0.008680817501520176 16-17 0.006386601447546987 18-19 0.011905121144941955 20-21 0.011078376620987655 22-23 0.014530035008496867 24-25 0.02178471820619587 26-27 0.013785964936937994 28-29 0.008184770787147595 30-31 0.008081427721653307 32-33 0.0098382598350562 34-35 0.011223056912679656 36-37 0.0098382598350562 38-39 0.016824251062470055 40-41 0.012773202895093974 42-43 0.015997506538515754 44-45 0.01775433865191865 46-47 0.01688625690176663 48-49 0.013682621871443708 50-51 0.026807191189218257 52-53 0.032036350303229225 54-55 0.02556707440328681 56-57 0.016018175151614612 58-59 0.01703093719345863 60-61 0.019759194122507828 62-63 0.026331813087944536 64-65 0.02126800287872443 66-67 0.020255240836880413 68-69 0.02953544811826746 70-71 0.05611528456339829 72-73 0.0683304349048231 74-75 0.07984285240088676 76-77 0.07723860715043071 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2419127.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.69095919441579 #Duplication Level Percentage of deduplicated Percentage of total 1 77.88546863482492 45.7117286148446 2 12.573896378273759 14.759480785041553 3 4.089618651507108 7.2007092418897605 4 1.8564436037129484 4.3582582316900345 5 0.9686733136622997 2.842618296243678 6 0.6026644451721438 2.1222572615714115 7 0.3832754341508162 1.5746362006177284 8 0.27751309611572667 1.303000784003528 9 0.20345412225404585 1.07468258284331 >10 1.0365649740563572 11.077031318029366 >50 0.08356725218750255 3.3671723084343963 >100 0.037517742478647066 3.827385637587822 >500 0.0011304013505312204 0.46710561555492874 >1k 1.4130016881621675E-4 0.10345570446590407 >5k 7.065008440810837E-5 0.2104774171819355 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5076 0.20982776017960197 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.133722619771513E-5 0.0 2 0.0 0.0 0.0 8.267445239543025E-5 0.0 3 0.0 0.0 0.0 8.267445239543025E-5 0.0 4 0.0 0.0 0.0 3.7203503577943616E-4 0.0 5 0.0 0.0 0.0 6.200583929657268E-4 0.0 6 0.0 0.0 0.0 0.0015294773693154597 0.0 7 0.0 0.0 0.0 0.002645582476653768 0.0 8 0.0 0.0 0.0 0.004175059845969228 0.0 9 0.0 0.0 0.0 0.006076572251064123 0.0 10 0.0 0.0 0.0 0.00822610801334531 0.0 11 0.0 0.0 0.0 0.01008628319224249 0.0 12 0.0 0.0 0.0 0.01314523793087341 0.0 13 0.0 0.0 0.0 0.016948262741063203 0.0 14 0.0 0.0 0.0 0.022487451051557028 4.133722619771513E-5 15 0.0 0.0 0.0 0.02831599994543486 4.133722619771513E-5 16 0.0 0.0 0.0 0.033607164898742395 4.133722619771513E-5 17 0.0 0.0 0.0 0.039477051018817946 4.133722619771513E-5 18 0.0 0.0 0.0 0.045594960496079785 4.133722619771513E-5 19 0.0 0.0 0.0 0.051919556104330196 4.133722619771513E-5 20 0.0 0.0 0.0 0.05807880280778975 4.133722619771513E-5 21 0.0 0.0 0.0 0.06481677067801732 4.133722619771513E-5 22 0.0 0.0 0.0 0.07374561153672378 4.133722619771513E-5 23 0.0 0.0 0.0 0.08275712684782568 4.133722619771513E-5 24 0.0 0.0 0.0 0.09131393267075272 8.267445239543025E-5 25 0.0 0.0 0.0 0.09937469177930716 8.267445239543025E-5 26 0.0 0.0 0.0 0.10896492825717707 8.267445239543025E-5 27 0.0 0.0 0.0 0.11880318809223327 8.267445239543025E-5 28 0.0 0.0 0.0 0.12822807566531233 8.267445239543025E-5 29 0.0 0.0 0.0 0.13736360265500736 8.267445239543025E-5 30 0.0 0.0 0.0 0.14749122307344756 1.2401167859314537E-4 31 0.0 0.0 0.0 0.15964436757557582 1.2401167859314537E-4 32 0.0 0.0 0.0 0.17097076755374976 1.2401167859314537E-4 33 0.0 0.0 0.0 0.183123912055878 1.2401167859314537E-4 34 0.0 0.0 0.0 0.19573176604618112 1.2401167859314537E-4 35 0.0 0.0 0.0 0.210117120762986 1.2401167859314537E-4 36 0.0 0.0 0.0 0.22561858058712916 1.2401167859314537E-4 37 0.0 0.0 0.0 0.24004527253013175 1.2401167859314537E-4 38 0.0 0.0 0.0 0.2547199878303206 1.653489047908605E-4 39 0.0 0.0 0.0 0.2705521454640455 1.653489047908605E-4 40 0.0 0.0 0.0 0.286590989228759 1.653489047908605E-4 41 0.0 0.0 0.0 0.30378727532700844 1.653489047908605E-4 42 0.0 0.0 0.0 0.32209966653259625 1.653489047908605E-4 43 0.0 0.0 0.0 0.34338833802441954 1.653489047908605E-4 44 0.0 0.0 0.0 0.3666198591475355 1.653489047908605E-4 45 0.0 0.0 0.0 0.3888592868419062 1.653489047908605E-4 46 0.0 0.0 0.0 0.41200813351262666 1.653489047908605E-4 47 0.0 0.0 0.0 0.4362317480644877 1.653489047908605E-4 48 0.0 0.0 0.0 0.46091007210452367 1.653489047908605E-4 49 0.0 0.0 0.0 0.48554705891836186 1.653489047908605E-4 50 0.0 0.0 0.0 0.5127882909826561 1.653489047908605E-4 51 0.0 0.0 0.0 0.5412696398328819 1.653489047908605E-4 52 0.0 0.0 0.0 0.5714458149572139 2.0668613098857562E-4 53 0.0 0.0 0.0 0.6060450732847015 2.0668613098857562E-4 54 0.0 0.0 0.0 0.641512413362341 2.4802335718629074E-4 55 0.0 0.0 0.0 0.6794599870118435 2.4802335718629074E-4 56 0.0 0.0 0.0 0.7205905270785701 2.4802335718629074E-4 57 0.0 0.0 0.0 0.7625891488954486 2.4802335718629074E-4 58 0.0 0.0 0.0 0.8096309123084484 2.4802335718629074E-4 59 0.0 0.0 0.0 0.8567966874000414 2.4802335718629074E-4 60 0.0 0.0 0.0 0.9025156595747144 2.4802335718629074E-4 61 0.0 0.0 0.0 0.9528644010835313 2.4802335718629074E-4 62 0.0 0.0 0.0 1.0065614579143634 2.4802335718629074E-4 63 0.0 0.0 0.0 1.0609612475905563 2.4802335718629074E-4 64 0.0 0.0 0.0 1.1191227248507416 2.4802335718629074E-4 65 0.0 0.0 0.0 1.1831954254572001 2.4802335718629074E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCGCG 270 0.0 24.982012 56 CGCGGAA 275 0.0 23.23878 59 TCGCGGA 275 0.0 23.2383 58 ACGCGAA 145 4.0233317E-7 22.034952 54 CGATCGT 330 0.0 21.51205 1 CGGTTAC 200 8.9858077E-10 21.29825 4 TAACGCG 135 5.3296244E-6 21.038353 52 CCTCGCG 360 0.0 20.70492 35 CTCGCGC 330 0.0 20.437294 36 CCGTATA 110 6.782447E-4 19.370857 66 CAATTCG 350 0.0 19.271837 54 TCGCGCT 370 0.0 19.187218 37 CGACACG 95 0.006651541 18.681517 2 AACGCGA 180 3.1965465E-6 17.75111 53 CGCTCCG 400 0.0 17.74744 25 AACTCGA 430 0.0 17.336504 13 AATTCGC 395 0.0 17.077019 55 CGCGCTT 420 0.0 16.903723 38 TTGCGTA 445 0.0 16.751085 36 TGCGTAA 390 0.0 16.38293 37 >>END_MODULE