FastQCFastQC Report
Thu 26 May 2016
SRR936030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419127
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57080.23595288713655796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41260.1705573952917726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29730.12289557348580708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA9100.045.45802100-101
CGGTATC7450.036.750957100-101
GGTATCA63100.035.1801071
CGTACAT12550.034.14726100-101
GTATCAA119950.030.6623821
TCGTATC3500.029.760284100-101
GCGTACA5000.029.760284100-101
AGCGTAC2650.029.19635698-99
ACGTATC3300.028.858458100-101
CGAGTAC9300.028.800278100-101
CCGGTAT4150.028.68461100-101
GCGTATC3750.027.776266100-101
ACGGATA1950.025.944864100-101
TCGAGTA4550.025.508816100-101
CCGTATC4800.025.420244100-101
CGATTTA1900.025.061293100-101
TACGCAG8450.025.005684100-101
CGGGTAT2500.024.998638100-101
CACGTAT3600.024.79818598-99
GGGTATC13850.024.710705100-101
Produced by FastQC (version 0.11.5)