FastQCFastQC Report
Thu 26 May 2016
SRR936029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916790
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161760.8439109135586058No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63460.3310743482593294No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCA651.9119987E-532.7646182
TTCGCGA703.172799E-530.4242925
TCGCGAT703.172799E-530.4242926
CGGTTAC2650.029.4683174
ATACGTA2001.8189894E-1224.8465021
CGATCGT2700.023.6633341
TACGTAG2153.6379788E-1223.1130262
GCCGATA1153.700589E-521.60396229
GCCTAGT3900.020.93513717
TCGCGCT3150.020.28338837
CTCGCGC3200.019.9654236
CGTCAAC900.004865725519.7199614
ATTCGCG2950.019.25253356
ACGTAGG2950.019.2510263
TCGCGGT2052.7632268E-819.04560333
CCTCGCG3850.017.51655835
CGCGGAA3104.0017767E-1117.17724259
GATCGTT3950.017.0735452
CGATCCG1250.0016025117.04160346
AATTCGC3357.2759576E-1216.95372255
Produced by FastQC (version 0.11.5)