FastQCFastQC Report
Thu 26 May 2016
SRR936029_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936029_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1916790
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150060.7828713630601161No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105240.5490429311505173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79640.41548630783758267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22590.1178532859624685No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14200.052.80843100-101
CGGTATC12000.045.37962100-101
CGTACAT16700.044.724785100-101
AGTATCA33400.041.606525100-101
GGTATCA103600.041.468581
ACGTATC4350.041.044315100-101
AGGTATC21700.040.59037100-101
TCGAGTA4800.039.676174100-101
CCGTACA7750.039.548187100-101
GGGTATC12450.038.242096100-101
TACGCAG9300.037.75636100-101
TCGTATC6300.036.842163100-101
CGAGTAC12300.036.53111100-101
ACCGTAT2350.035.45530398-99
TCGGTAT2900.034.88767100-101
GCGTACA5000.034.518272100-101
CCGTATC6600.034.265785100-101
GTATCAA211850.033.0590021
CCGGTAT5050.032.99801100-101
TCGTACA7850.032.979237100-101
Produced by FastQC (version 0.11.5)