FastQCFastQC Report
Thu 26 May 2016
SRR936028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921174
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164330.8553623982002672No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63680.33146399024762985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGC2550.030.62519336
ATTCGCG2950.027.67736656
CGGTTAC2600.027.3042584
CCTCGCG3150.027.04421835
CGATCGT2950.026.4698681
AATTCGC3250.025.12318855
TAGCGCG851.1900405E-425.0563245
CGCGGAA3150.024.79440759
AACTCGA3650.024.31327413
CGCGCTT3250.024.02962538
ACTCGAG3550.023.99823214
TCGCGCT3300.023.66553737
CGCTCCG3700.023.02353125
TCGCGGA3550.023.000758
CAATTCG3600.022.68006554
GATCGTT3850.021.2045732
ATCGTTC4200.021.1277453
CTCGAGG4150.019.67376115
CGGTATA1106.797399E-419.36422510
GCGTCGT1106.805742E-419.3606973
Produced by FastQC (version 0.11.5)