##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936028_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1921174 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.61500259737015 39.0 36.0 41.0 30.0 41.0 2 36.47753977515831 39.0 36.0 41.0 30.0 41.0 3 36.332515430668956 39.0 36.0 41.0 30.0 41.0 4 36.22565681192854 39.0 35.0 41.0 29.0 41.0 5 36.13526468711319 39.0 35.0 41.0 29.0 41.0 6 36.00463050197431 39.0 35.0 40.0 29.0 41.0 7 35.804678805771886 39.0 35.0 40.0 28.0 41.0 8 35.632427880035856 38.0 35.0 40.0 28.0 41.0 9 35.7430461790551 39.0 35.0 40.0 29.0 41.0 10-11 35.90037445853421 39.0 35.0 41.0 29.0 41.0 12-13 35.75005985923191 39.0 35.0 41.0 27.5 41.0 14-15 35.555480398964384 39.0 35.0 41.0 27.0 41.0 16-17 35.33588888877322 39.0 35.0 41.0 26.0 41.0 18-19 35.08725966518389 39.0 35.0 41.0 25.0 41.0 20-21 34.74450726482869 39.0 34.0 41.0 23.5 41.0 22-23 34.2859832061021 38.0 34.0 41.0 21.0 41.0 24-25 33.92251300506878 38.0 33.0 40.0 17.5 41.0 26-27 32.91786870944537 37.5 32.0 39.5 14.0 40.5 28-29 32.89551935431148 37.5 32.5 39.5 12.0 40.5 30-31 33.265007490211715 38.0 33.0 40.0 10.0 41.0 32-33 33.123615299811476 38.0 33.0 40.0 8.0 41.0 34-35 32.84677988563243 37.0 32.5 40.0 7.0 41.0 36-37 32.5229240037602 37.0 32.0 40.0 3.5 41.0 38-39 32.202827021394214 36.0 32.0 40.0 2.0 41.0 40-41 31.828439277233606 36.0 31.0 39.0 2.0 41.0 42-43 31.45406871006999 35.0 31.0 39.0 2.0 41.0 44-45 31.08998013714531 35.0 31.0 38.5 2.0 40.0 46-47 30.680940404148714 35.0 30.5 37.5 2.0 40.0 48-49 30.276153279192826 35.0 30.0 37.0 2.0 39.5 50-51 29.836215251715878 35.0 29.5 36.5 2.0 39.0 52-53 29.372547202908223 34.5 29.0 36.0 2.0 39.0 54-55 28.92463930908913 34.0 29.0 35.5 2.0 37.5 56-57 28.49948963498361 34.0 28.5 35.0 2.0 37.0 58-59 28.0834101960572 34.0 27.5 35.0 2.0 36.5 60-61 27.733875224211864 34.0 27.0 35.0 2.0 36.0 62-63 27.357660992705505 34.0 26.0 35.0 2.0 36.0 64-65 26.978381968525497 33.5 25.5 35.0 2.0 35.5 66-67 26.626008367800104 33.0 25.0 35.0 2.0 35.0 68-69 26.253833072902296 33.0 24.5 35.0 2.0 35.0 70-71 25.807015137618976 33.0 23.0 35.0 2.0 35.0 72-73 25.33373681925739 33.0 19.0 35.0 2.0 35.0 74-75 24.77333599143024 32.5 9.5 35.0 2.0 35.0 76-77 23.110850969251093 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9307796539233948 1101 2 0.8434953755794226 1101 3 0.9844664078248542 1101 4 0.8669617011267903 1101 5 0.8451796300749805 1101 6 1.02391748007512 1101 7 0.8861098863678478 1101 8 0.9032365991835931 1101 9 0.863160533964205 1101 10-11 0.8478385469968117 1101 12-13 0.9236787766400099 1101 14-15 0.9645835968987768 1101 16-17 1.0980154994597342 1101 18-19 1.1188110817219155 1101 20-21 1.0329467556270657 1101 22-23 1.038193201525992 1101 24-25 1.0553014311383677 1101 26-27 1.0454478039923885 1101 28-29 1.0990581105717823 1101 30-31 1.0678035642532535 1101 32-33 1.0900023874796645 1101 34-35 0.9970737963961795 1101 36-37 1.017183675992932 1101 38-39 1.1377241298279088 1101 40-41 1.1158314846828397 1101 42-43 1.1435956254333703 1101 44-45 0.9660607330411679 1101 46-47 0.9569204283307116 1101 48-49 0.8897149441982783 1101 50-51 0.8106482711583922 1101 52-53 0.8534212710163835 1101 54-55 0.8909094877031656 1101 56-57 0.9147613155911181 1101 58-59 0.9496796782760342 1101 60-61 0.838136301349401 1101 62-63 0.9231608625303842 1101 64-65 0.8895728433021866 1101 66-67 0.8470708294011686 1101 68-69 0.7144086990802627 1101 70-71 0.5012137648462947 1101 72-73 0.6063889732527201 1101 74-75 0.6856437039442476 1101 76-77 0.8986646457748186 1102 1 0.9654422733972652 1102 2 0.9777360854420252 1102 3 0.95630710847378 1102 4 0.9099153499347068 1102 5 0.8594985794087151 1102 6 0.9658796348981582 1102 7 1.006210801721032 1102 8 1.008139426886558 1102 9 0.9946883279156822 1102 10-11 0.8841477912577815 1102 12-13 0.968908971394093 1102 14-15 1.0093563070121974 1102 16-17 0.9909273028193937 1102 18-19 1.074056287090933 1102 20-21 1.1342450522485876 1102 22-23 1.0266687475323053 1102 24-25 1.1081610620538314 1102 26-27 0.9954354551062821 1102 28-29 1.053020759350268 1102 30-31 1.2249584960656392 1102 32-33 1.3159089428826576 1102 34-35 1.0963572410827709 1102 36-37 1.128643890185323 1102 38-39 1.2598184625209754 1102 40-41 1.3002195493884408 1102 42-43 1.229664802229749 1102 44-45 1.0952393593587573 1102 46-47 1.0988711940256373 1102 48-49 1.1746776480028878 1102 50-51 1.1181857625045737 1102 52-53 1.0471778533565832 1102 54-55 1.2063784190574403 1102 56-57 1.254778936747769 1102 58-59 1.288173843587309 1102 60-61 1.1007571188840508 1102 62-63 0.9707347862897677 1102 64-65 0.9644736363182176 1102 66-67 0.906723627351635 1102 68-69 0.8625857347589445 1102 70-71 0.8689763898227802 1102 72-73 1.0402206982449442 1102 74-75 1.1349179349541565 1102 76-77 1.1201192037934682 1103 1 0.21565706477532842 1103 2 0.4092886982612356 1103 3 0.29471309016501834 1103 4 0.5649276727679151 1103 5 0.24432933535245382 1103 6 0.3265218980132687 1103 7 0.39068351856408157 1103 8 0.25476259843284055 1103 9 0.33643921611572836 1103 10-11 0.32491525897811613 1103 12-13 0.5789208162001884 1103 14-15 0.4876874020664488 1103 16-17 0.4396893730040432 1103 18-19 0.4115240202606856 1103 20-21 0.5816530865690055 1103 22-23 0.46399730743873135 1103 24-25 0.7735364673342033 1103 26-27 0.6539550211731608 1103 28-29 0.7432973670132057 1103 30-31 0.8529003679684877 1103 32-33 0.7422181757357578 1103 34-35 0.7601314159405987 1103 36-37 0.7796890692016731 1103 38-39 0.6619916571986408 1103 40-41 0.7938726967638807 1103 42-43 0.6821423301430016 1103 44-45 0.5200254823278634 1103 46-47 0.5792393583181017 1103 48-49 0.5757438409628648 1103 50-51 0.4892758054885107 1103 52-53 0.6252789820780542 1103 54-55 0.47418541031627726 1103 56-57 0.5028551182286769 1103 58-59 0.32270745274335866 1103 60-61 0.1764719619147037 1103 62-63 0.2855655294005608 1103 64-65 0.29181874430422283 1103 66-67 0.4160618389142492 1103 68-69 0.2955486879783784 1103 70-71 0.32352339015450937 1103 72-73 0.2512475325697281 1103 74-75 0.3418574770362639 1103 76-77 0.281131889798953 1104 1 0.44336163926102046 1104 2 0.482944882577371 1104 3 0.614770306371696 1104 4 0.7288108792490817 1104 5 0.6575056156119246 1104 6 0.6260103251609834 1104 7 0.4397820197618074 1104 8 0.5648297775052029 1104 9 0.5387097500816012 1104 10-11 0.4906945330943415 1104 12-13 0.627221975827787 1104 14-15 0.664214234655951 1104 16-17 0.7154564980458176 1104 18-19 0.560012100738156 1104 20-21 0.6045142716815519 1104 22-23 0.7174298113660953 1104 24-25 0.7534141333377207 1104 26-27 0.4263125974796509 1104 28-29 0.5282589053546118 1104 30-31 0.8135378352144045 1104 32-33 0.7062222483303842 1104 34-35 0.7800504325214277 1104 36-37 0.662388818412289 1104 38-39 0.758226537222626 1104 40-41 0.9339666725094524 1104 42-43 0.7325670792755474 1104 44-45 0.7523379602705766 1104 46-47 0.6349028882957093 1104 48-49 0.618547622479408 1104 50-51 0.5876409898015282 1104 52-53 0.6030360255742409 1104 54-55 0.5008162439801467 1104 56-57 0.5278369258485505 1104 58-59 0.4796353921791301 1104 60-61 0.4700624662730668 1104 62-63 0.269583094026455 1104 64-65 0.3443100853559109 1104 66-67 0.3430240393646038 1104 68-69 0.1692353230595316 1104 70-71 0.20176998825750658 1104 72-73 0.09438096313730071 1104 74-75 -0.13444667457419257 1104 76-77 0.17954376528499338 1105 1 0.15809158522002065 1105 2 0.23794895829514928 1105 3 0.18913555106571778 1105 4 0.3173113170113666 1105 5 0.12656035099217178 1105 6 0.20236512394128425 1105 7 0.2816200175277146 1105 8 0.2153052779127762 1105 9 0.1871081439468938 1105 10-11 0.21110331249234093 1105 12-13 0.4129796064771867 1105 14-15 0.3500201701086567 1105 16-17 0.2971323728156179 1105 18-19 0.14331222451921377 1105 20-21 0.19381626727373913 1105 22-23 0.16793550227573917 1105 24-25 0.1688822370722889 1105 26-27 0.06433753483436533 1105 28-29 0.22513267886547084 1105 30-31 0.26066369941752043 1105 32-33 0.35272316914069535 1105 34-35 0.34805302890460155 1105 36-37 0.36436025819732976 1105 38-39 0.2800150225651379 1105 40-41 0.43386704385262576 1105 42-43 0.46670046091368533 1105 44-45 0.22223953923383277 1105 46-47 0.2772043102683561 1105 48-49 0.22760930167513393 1105 50-51 0.22178946669070143 1105 52-53 0.0775401465777712 1105 54-55 0.025975498878555214 1105 56-57 -0.07905556577358297 1105 58-59 0.15168804441473682 1105 60-61 0.07213806329024663 1105 62-63 0.10575584407928673 1105 64-65 0.14628454419116466 1105 66-67 0.024390461488206228 1105 68-69 -0.054941230996561785 1105 70-71 -0.02346172384586609 1105 72-73 -0.4186130289527874 1105 74-75 -0.5330624400543726 1105 76-77 -0.47371077083794333 1106 1 0.0072710158013791215 1106 2 -0.23259488765852865 1106 3 0.10140147118918463 1106 4 0.0046523849629451774 1106 5 -0.15428166359772177 1106 6 -0.10813313980739991 1106 7 -0.5474215896936556 1106 8 -0.2413745788801407 1106 9 -0.2212608565645624 1106 10-11 -0.23692130907664932 1106 12-13 0.02225262414477669 1106 14-15 0.1285525666738394 1106 16-17 0.23147829509257178 1106 18-19 0.2814397649644462 1106 20-21 0.32024183278917917 1106 22-23 0.4117437698827686 1106 24-25 0.1674456797849615 1106 26-27 0.1708105668585489 1106 28-29 0.22046588657855892 1106 30-31 0.4651958985794522 1106 32-33 0.29391090860429614 1106 34-35 0.16204443289035453 1106 36-37 0.1882599594006038 1106 38-39 0.22915811300273958 1106 40-41 0.25210845008160376 1106 42-43 0.32542119812509185 1106 44-45 0.2115918771164189 1106 46-47 0.3305651005991841 1106 48-49 0.14696603626646265 1106 50-51 -0.0240623731973173 1106 52-53 0.07591022383422441 1106 54-55 0.15760892104335156 1106 56-57 0.13929216902412378 1106 58-59 0.12696867108032706 1106 60-61 -0.14241504267043226 1106 62-63 -0.12850573317267688 1106 64-65 -0.08605594052354348 1106 66-67 -0.2095673839450498 1106 68-69 -0.12214761609441283 1106 70-71 -0.2413460096498845 1106 72-73 -0.29092946636249906 1106 74-75 -0.3306819363171698 1106 76-77 -0.4368012981738083 1107 1 -0.24473310050851893 1107 2 -0.16054441031484856 1107 3 -0.120820182952734 1107 4 -0.1872547998043217 1107 5 -0.13350867104440312 1107 6 -0.31152662432394607 1107 7 -0.2191714389393482 1107 8 -0.21206930416928316 1107 9 -0.25870643802173987 1107 10-11 -0.17101476226356738 1107 12-13 -0.1564296510963814 1107 14-15 -0.2291586548152722 1107 16-17 -0.22403178563448023 1107 18-19 -0.14250763193536642 1107 20-21 -0.1662446728892064 1107 22-23 -0.1597685933909574 1107 24-25 -0.2940852000663341 1107 26-27 -0.512905894035228 1107 28-29 -0.28800617906653514 1107 30-31 -0.13701694463691183 1107 32-33 -0.033689216259752186 1107 34-35 -0.07691542343077629 1107 36-37 0.13841645132291802 1107 38-39 0.2054276943758424 1107 40-41 0.09916305310850149 1107 42-43 0.07063916911834411 1107 44-45 0.1296894670641251 1107 46-47 0.02360702577789553 1107 48-49 0.07071929172166591 1107 50-51 -0.0354121916530552 1107 52-53 -0.11587738002860704 1107 54-55 -0.10703668631828478 1107 56-57 -0.149608621147177 1107 58-59 -0.06475502374807007 1107 60-61 -0.08287198942625551 1107 62-63 -0.09177011007805547 1107 64-65 -0.25932895508997333 1107 66-67 -0.11731937939813619 1107 68-69 -0.2877299594768026 1107 70-71 -0.5278320324600081 1107 72-73 -0.5287934957731508 1107 74-75 -0.7319329627672744 1107 76-77 -0.5811419827503421 1108 1 0.25094154586791717 1108 2 0.3961099686219285 1108 3 0.2973039836755902 1108 4 0.3811370698129295 1108 5 0.2996171832737815 1108 6 0.3197271044865033 1108 7 0.32941447910409494 1108 8 0.2959213554182156 1108 9 0.28789574621335134 1108 10-11 0.41816960608425546 1108 12-13 0.5460467179206603 1108 14-15 0.4763107370870543 1108 16-17 0.28089283753455874 1108 18-19 0.23985400076958996 1108 20-21 0.20269709901758404 1108 22-23 0.2871116008436587 1108 24-25 0.268245881329257 1108 26-27 0.15938729213971214 1108 28-29 0.236096931469568 1108 30-31 0.366879371358209 1108 32-33 0.337594175462236 1108 34-35 0.4832592983530901 1108 36-37 0.4448257893947343 1108 38-39 0.5189154883484122 1108 40-41 0.3432002147359974 1108 42-43 0.29511029978012004 1108 44-45 0.3653877916362731 1108 46-47 0.21256910848028454 1108 48-49 0.357724585136328 1108 50-51 0.31750596908683804 1108 52-53 0.4097325489607435 1108 54-55 0.6078470625715475 1108 56-57 0.5125250444221159 1108 58-59 0.4618943838847578 1108 60-61 0.4556356671531958 1108 62-63 0.579786956049329 1108 64-65 0.6387352420258168 1108 66-67 0.7480977938636428 1108 68-69 0.7309145582442049 1108 70-71 0.7736552093227793 1108 72-73 0.6364660533729349 1108 74-75 0.7515521473927258 1108 76-77 0.9542806193136677 1109 1 -0.10728340041082873 1109 2 -0.2432637695434181 1109 3 -0.3125696809854688 1109 4 -0.2708336473391384 1109 5 -0.1616720899120878 1109 6 -0.2920150682267604 1109 7 -0.13783902970682504 1109 8 -0.08739051396031527 1109 9 -0.08914813992574011 1109 10-11 -0.1058323726144863 1109 12-13 0.047172424988566775 1109 14-15 0.1572608175473036 1109 16-17 0.2066671769886952 1109 18-19 0.07118584332891231 1109 20-21 0.06365353845385613 1109 22-23 0.14457533719178173 1109 24-25 0.008599396215124955 1109 26-27 -0.021111637643045356 1109 28-29 0.07798393788298341 1109 30-31 0.20209366069792623 1109 32-33 0.23600519411076704 1109 34-35 0.3363695818797936 1109 36-37 0.4042942862963699 1109 38-39 0.25392528059293795 1109 40-41 0.33701073250339064 1109 42-43 0.315160929642218 1109 44-45 0.2286428510064411 1109 46-47 0.21480829381603428 1109 48-49 0.2683813663741397 1109 50-51 0.24537182782867717 1109 52-53 0.17560178390334258 1109 54-55 0.23312152023741461 1109 56-57 0.24040123847239414 1109 58-59 0.3550082282276463 1109 60-61 0.3329785671776264 1109 62-63 0.16698592704182857 1109 64-65 0.3179694771958985 1109 66-67 0.23876998075968103 1109 68-69 0.1444844237458156 1109 70-71 0.2483659193058685 1109 72-73 -0.03569296626125862 1109 74-75 -0.010598348899314658 1109 76-77 0.3086392923862995 1110 1 -0.09845226588640088 1110 2 -0.24457822884979663 1110 3 -0.3907107942245531 1110 4 -0.22092381797754257 1110 5 -0.20467916640218675 1110 6 -0.23108994287687068 1110 7 -0.2830312836679809 1110 8 -0.3726025292613855 1110 9 -0.3620451320364424 1110 10-11 -0.33472393416674606 1110 12-13 -0.31976670094044835 1110 14-15 -0.16300801795605224 1110 16-17 -0.377537594181085 1110 18-19 -0.22941271190885715 1110 20-21 -0.5018283791581197 1110 22-23 -0.546200460500863 1110 24-25 -0.5612048699990808 1110 26-27 -0.591682862287314 1110 28-29 -0.1912066599864417 1110 30-31 -0.11751489713066121 1110 32-33 -0.05676527970418732 1110 34-35 -0.056127840001963136 1110 36-37 -0.07702642145424221 1110 38-39 -0.20233497472967343 1110 40-41 -0.15485231988702353 1110 42-43 -0.27269384736960234 1110 44-45 -0.24077647548239156 1110 46-47 -0.16728927843477592 1110 48-49 -0.08522952319096078 1110 50-51 -0.23554565206707778 1110 52-53 -0.3313220511174073 1110 54-55 -0.14785450707286074 1110 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20-21 -0.6587212755561396 2314 22-23 -0.5899304489011996 2314 24-25 -0.4751222994778743 2314 26-27 -0.36169391889978186 2314 28-29 -0.6965029728560026 2314 30-31 -0.801858929383819 2314 32-33 -0.7661813211328123 2314 34-35 -0.8577931743721621 2314 36-37 -1.3338808695142248 2314 38-39 -0.8400533373952506 2314 40-41 -0.7149913855706878 2314 42-43 -0.6721574170262627 2314 44-45 -0.6997259235206528 2314 46-47 -0.41302292485778125 2314 48-49 -0.38830039412184547 2314 50-51 -0.27983129497976833 2314 52-53 -0.26924014964072995 2314 54-55 -0.3060303180721249 2314 56-57 -0.31653821203056864 2314 58-59 -0.2804648747306082 2314 60-61 -0.22070272346072173 2314 62-63 -0.34493015327838705 2314 64-65 -0.23191591761621666 2314 66-67 -0.12574824856821465 2314 68-69 -0.05268242704410753 2314 70-71 -0.08599046691935541 2314 72-73 -0.07010682956038039 2314 74-75 0.09676321926797726 2314 76-77 0.06275414882531649 2315 1 -1.2092289593192902 2315 2 -1.0756062823940624 2315 3 -0.9204425851376286 2315 4 -0.8139275025824588 2315 5 -0.9527590482820045 2315 6 -1.0133428726033387 2315 7 -1.3089780409052807 2315 8 -1.4346870135718888 2315 9 -0.961968424858334 2315 10-11 -1.3073298277610377 2315 12-13 -1.143095437755015 2315 14-15 -0.9581767959175949 2315 16-17 -0.8812748521433349 2315 18-19 -0.8465644147638685 2315 20-21 -1.0358063336038157 2315 22-23 -0.970278422484256 2315 24-25 -1.1718835733373894 2315 26-27 -1.0449430282053882 2315 28-29 -1.1146090002204687 2315 30-31 -1.0865033600163514 2315 32-33 -1.060271307646886 2315 34-35 -1.0350496607554618 2315 36-37 -1.2323252025638745 2315 38-39 -0.977862835397513 2315 40-41 -0.9788215394690774 2315 42-43 -0.9704936150310957 2315 44-45 -0.9582842237941662 2315 46-47 -0.8664045963705114 2315 48-49 -0.8980655604892718 2315 50-51 -0.7836533569262372 2315 52-53 -0.7034598313978968 2315 54-55 -0.9121029762547543 2315 56-57 -0.8893688251541114 2315 58-59 -0.631129552535139 2315 60-61 -0.7240441415409116 2315 62-63 -0.7608140378947787 2315 64-65 -0.6354165416082829 2315 66-67 -0.6238829534330321 2315 68-69 -0.7085037886574774 2315 70-71 -0.7669966042659944 2315 72-73 -0.834717096357025 2315 74-75 -0.8419025165032679 2315 76-77 -1.005393625386823 2316 1 -1.7089212272210332 2316 2 -1.347016413844763 2316 3 -1.3064296388362848 2316 4 -2.474176897296168 2316 5 -1.7986822565507765 2316 6 -1.6481235446327673 2316 7 -1.6482784233552152 2316 8 -1.4269925137143744 2316 9 -1.6369937498928806 2316 10-11 -1.562952224910262 2316 12-13 -2.135665563553978 2316 14-15 -3.5380793753591746 2316 16-17 -2.56510227310887 2316 18-19 -2.1491033841846416 2316 20-21 -1.933042971205559 2316 22-23 -2.3912703017095893 2316 24-25 -2.3381211712548726 2316 26-27 -2.2499476860959007 2316 28-29 -2.734443710964271 2316 30-31 -2.925114039397826 2316 32-33 -2.938129204866758 2316 34-35 -2.8160761209263043 2316 36-37 -2.8982550683831008 2316 38-39 -2.9710244773697774 2316 40-41 -2.5659612115248507 2316 42-43 -2.829425389507776 2316 44-45 -2.899880982189746 2316 46-47 -2.8084815126314595 2316 48-49 -2.601008293581369 2316 50-51 -2.6002186835413497 2316 52-53 -2.8467061296322136 2316 54-55 -3.0401722147794565 2316 56-57 -2.8588542539793913 2316 58-59 -2.9136431849473503 2316 60-61 -2.71275983300416 2316 62-63 -2.684866474486398 2316 64-65 -2.7360138053071523 2316 66-67 -2.626945905601648 2316 68-69 -2.7656952602874547 2316 70-71 -2.605874304106262 2316 72-73 -2.5587161288568865 2316 74-75 -2.5139511022527543 2316 76-77 -2.958061554912579 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47300.0 3 5229.0 4 7672.0 5 9851.0 6 10763.0 7 10827.0 8 13317.0 9 14836.0 10 19978.0 11 23172.0 12 15886.0 13 11116.0 14 10469.0 15 10434.0 16 10284.0 17 10907.0 18 11328.0 19 11815.0 20 12615.0 21 13752.0 22 15199.0 23 17237.0 24 20068.0 25 23273.0 26 27103.0 27 31616.0 28 37085.0 29 43598.0 30 50988.0 31 61102.0 32 75347.0 33 94368.0 34 127927.0 35 168523.0 36 230798.0 37 297568.0 38 253123.0 39 64182.0 40 518.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.122141036392037 25.73177860473154 30.674970212486862 21.471110146389556 2 21.9402078373948 26.117621827142962 30.33735706168751 21.604813273774727 3 22.091511161688043 26.081003838733626 30.002457122688508 21.825027876889823 4 21.673012116312012 26.2261173605359 30.33175256755132 21.769117955600766 5 22.060660138820417 26.077945795171946 30.07451198631474 21.786882079692894 6 22.020436232561575 26.04656562385796 30.403268880973627 21.529729262606836 7 22.0466037876315 25.76285992885371 30.25659206874525 21.933944214769546 8 21.73079836506729 26.348947034406784 29.963666426664126 21.956588173861796 9 21.818634275046257 25.972732564011526 30.28848006014079 21.920153100801425 10-11 22.22773507916778 26.149979500328648 29.987179570613232 21.635105849890344 12-13 22.402918819550322 25.90462763366795 29.636529809688945 22.055923737092787 14-15 22.190491261897375 26.022143536844123 29.762281547434373 22.02508365382413 16-17 22.285992407787937 26.122727314134607 29.68833409897465 21.90294617910281 18-19 22.282167051115266 26.250707338569327 29.326348186011693 22.14077742430371 20-21 22.160082813212945 26.478337926114143 29.207899530935094 22.153679729737817 22-23 22.36718978369136 26.55790455411412 28.700401558757022 22.374504103437495 24-25 22.49719897872057 26.8286816248839 28.066259251700398 22.607860144695135 26-27 22.531767677530155 26.958731302374566 27.81405997920765 22.695441040887626 28-29 22.66659101219694 27.089982923085692 27.79263248663662 22.45079357808075 30-31 22.350062193473626 26.96841237851841 27.830714023762877 22.850811404245086 32-33 22.47291117363658 27.269279722227285 27.60072254222421 22.657086561911928 34-35 22.659267910441354 26.852029473904498 27.72474711126532 22.763955504388836 36-37 22.537337518675265 26.96877651627546 27.99577301287357 22.4981129521757 38-39 22.53663155982207 26.91167271997482 28.093362809859485 22.45833291034362 40-41 22.38285001835044 27.066350501188225 28.001884498954936 22.548914981506403 42-43 22.380072821017933 27.273053828621762 27.67570907826333 22.671164272096973 44-45 22.387212736243814 27.10624512740018 27.93211744478588 22.574424691570126 46-47 22.233475952894675 27.079391382280033 27.951325575807935 22.735807089017356 48-49 22.367449399330575 27.229913501416135 27.67165990290481 22.73097719634848 50-51 22.087836736733973 27.49465422869124 27.680239832100867 22.737269202473918 52-53 22.123917535489007 27.33318077362352 27.71546520688372 22.827436484003748 54-55 22.170669890487957 27.33602089236123 27.672095931591677 22.821213285559132 56-57 22.049676749466453 27.558250040281045 27.632044400517785 22.760028809734713 58-59 22.078894321674593 27.580631054617545 27.500874613712277 22.839600009995586 60-61 22.037125252825003 27.325508448324783 27.441163684828418 23.1962026140218 62-63 22.142299863564194 27.270396189689766 27.483809800000884 23.103494146745156 64-65 22.092842855610755 27.2212168509481 27.444275648507855 23.241664644933294 66-67 21.987540097193804 27.2944393264999 27.59626645439417 23.121754121912126 68-69 22.14208562438122 27.16874513012666 27.540710383865125 23.148458861627 70-71 21.914859266241866 27.035047379974635 27.605950685245418 23.44414266853808 72-73 22.172922959289174 26.850206806892206 27.46880447855026 23.508065755268355 74-75 22.040404056189473 26.867762316027616 27.395088825840304 23.69674480194261 76-77 21.970983071895866 26.770100853932398 27.823803319985814 23.435112754185923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 17899.0 1 10753.5 2 3299.5 3 3006.0 4 3021.0 5 3137.5 6 3302.0 7 3517.0 8 3675.0 9 3666.0 10 3654.0 11 3797.0 12 4115.0 13 4278.0 14 4542.0 15 4971.5 16 5434.0 17 5731.0 18 6131.5 19 7097.0 20 8157.5 21 9483.5 22 10314.0 23 11420.0 24 13782.0 25 16840.0 26 18642.0 27 19947.0 28 23524.0 29 26959.0 30 28122.0 31 31004.5 32 37043.0 33 42254.0 34 46275.5 35 48242.0 36 51255.5 37 56628.0 38 60193.5 39 61400.0 40 63499.0 41 68576.0 42 76102.5 43 80651.0 44 80861.5 45 81052.5 46 83756.0 47 87241.0 48 88003.0 49 88053.0 50 88219.0 51 86166.0 52 83997.0 53 82534.5 54 77936.0 55 70911.0 56 63665.0 57 60308.0 58 58367.0 59 51438.0 60 43309.5 61 40169.0 62 36323.0 63 26868.5 64 18714.0 65 16168.0 66 13996.5 67 10787.5 68 8331.5 69 5989.5 70 5066.0 71 4174.5 72 2877.0 73 2178.5 74 1886.0 75 1712.5 76 1181.5 77 652.0 78 480.0 79 405.5 80 284.0 81 200.0 82 135.5 83 108.0 84 89.5 85 66.5 86 56.5 87 51.0 88 47.0 89 36.5 90 28.0 91 26.0 92 26.0 93 24.0 94 23.5 95 23.0 96 21.0 97 18.0 98 17.0 99 40.0 100 61.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034874509024169593 2 0.00796388041895216 3 0.011867743369418907 4 0.01951931475233373 5 0.014314164151711401 6 0.014158009633692733 7 0.017853666560134585 8 0.0187905936682466 9 0.018113924090165698 10-11 0.02141919472156088 12-13 0.020690473637473752 14-15 0.00843234397300817 16-17 0.0065845155097872445 18-19 0.01254441294749981 20-21 0.011685563098397125 22-23 0.014808653458770523 24-25 0.023214971678775582 26-27 0.014314164151711401 28-29 0.008302215207992613 30-31 0.00793785466594905 32-33 0.009317219575113967 34-35 0.011477357074372233 36-37 0.009577477105145083 38-39 0.01683866219301323 40-41 0.012440309935487365 42-43 0.015615451801866983 44-45 0.0185563618912186 46-47 0.01715097122905057 48-49 0.014340189904714515 50-51 0.025869598485092966 52-53 0.0319856504408242 54-55 0.024568310834937386 56-57 0.015381220024838977 58-59 0.01696879095802879 60-61 0.01972752077635862 62-63 0.025531263696052516 64-65 0.019779572282364846 66-67 0.01938918598731817 68-69 0.02938307514051304 70-71 0.05512254486059045 72-73 0.06594925810988489 74-75 0.0775827697022758 76-77 0.07635955931112955 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1921174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.11169806097862 #Duplication Level Percentage of deduplicated Percentage of total 1 74.85219480858328 38.258227802578595 2 12.217133300591065 12.488768568610753 3 4.8829389464197135 7.487259032387923 4 2.562938791701361 5.239846146808373 5 1.4596928164115686 3.7303689247103797 6 0.9450447741759167 2.8981705891071106 7 0.6262315143327969 2.2405429253793123 8 0.43141489975418973 1.7640278476194802 9 0.3170948633565875 1.4586531221312256 >10 1.5414442088736 13.890591054809983 >50 0.10669893364077324 3.7387083970649138 >100 0.05522935940344622 4.9945639123895225 >500 0.0016360275837354727 0.5620319189338788 >1k 1.0225172398346704E-4 0.05660038759581464 >5k 1.0225172398346704E-4 0.33280818855969313 >10k+ 1.0225172398346704E-4 0.8588311813130398 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16433 0.8553623982002672 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6368 0.33146399024762985 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.205150600622328E-5 0.0 2 0.0 0.0 0.0 5.72566566068456E-4 0.0 3 0.0 0.0 0.0 9.36927108112019E-4 0.0 4 5.205150600622328E-5 0.0 0.0 0.002082060240248931 0.0 5 2.082060240248931E-4 0.0 0.0 0.004164120480497862 0.0 6 2.602575300311164E-4 0.0 0.0 0.007495416864896152 0.0 7 2.602575300311164E-4 0.0 0.0 0.015979812343910545 0.0 8 2.602575300311164E-4 0.0 0.0 0.025765495473080522 0.0 9 2.602575300311164E-4 0.0 0.0 0.03456219998813226 0.0 10 2.602575300311164E-4 0.0 0.0 0.0445040376353209 0.0 11 3.6436054204356296E-4 0.0 0.0 0.05637178100473981 0.0 12 4.164120480497862E-4 0.0 0.0 0.07318441744474993 0.0 13 4.164120480497862E-4 0.0 0.0 0.09587887406346328 0.0 14 6.246180720746793E-4 0.0 0.0 0.12534002646298564 0.0 15 6.766695780809027E-4 0.0 0.0 0.15880914482498723 0.0 16 6.766695780809027E-4 0.0 0.0 0.19264262372903235 0.0 17 7.287210840871259E-4 0.0 0.0 0.22402968185078498 0.0 18 7.807725900933492E-4 0.0 0.0 0.257134439670743 0.0 19 0.0010410301201244656 0.0 0.0 0.2890420128525579 0.0 20 0.001145133132136912 0.0 0.0 0.3261026851289888 0.0 21 0.001145133132136912 0.0 0.0 0.3656618296937185 0.0 22 0.0014574421681742519 0.0 0.0 0.4078235495587594 0.0 23 0.0015615451801866984 0.0 0.0 0.45336861731420475 0.0 24 0.0015615451801866984 0.0 0.0 0.5021408784420359 0.0 25 0.0015615451801866984 0.0 0.0 0.5508610880638609 0.0 26 0.0015615451801866984 0.0 0.0 0.6073369720806132 0.0 27 0.0015615451801866984 0.0 0.0 0.6614184868210792 0.0 28 0.0015615451801866984 0.0 0.0 0.7123248596951656 0.0 29 0.0015615451801866984 0.0 0.0 0.7618258419070839 0.0 30 0.0015615451801866984 0.0 0.0 0.8165319747196246 0.0 31 0.0015615451801866984 0.0 0.0 0.875610434036688 0.0 32 0.0016135966861929216 0.0 0.0 0.935990181003907 0.0 33 0.0016135966861929216 0.0 0.0 1.003813293330016 0.0 34 0.0016135966861929216 0.0 0.0 1.0765292472207098 0.0 35 0.0016135966861929216 0.0 0.0 1.154190094181995 0.0 36 0.0016135966861929216 0.0 0.0 1.242105087826506 0.0 37 0.0016135966861929216 0.0 0.0 1.3325706052653221 0.0 38 0.0016656481921991449 0.0 0.0 1.4214225260179454 0.0 39 0.0016656481921991449 0.0 0.0 1.5054857082179958 0.0 40 0.0016656481921991449 0.0 0.0 1.5948581440306813 0.0 41 0.0016656481921991449 0.0 0.0 1.6897480394800262 0.0 42 0.0017697512042115914 0.0 0.0 1.7893225704699314 0.0 43 0.0017697512042115914 0.0 0.0 1.8998799692271497 0.0 44 0.0018218027102178147 0.0 0.0 2.021212029727656 0.0 45 0.0018218027102178147 0.0 0.0 2.148425910406866 0.0 46 0.0018218027102178147 0.0 0.0 2.29307704559816 0.0 47 0.0018218027102178147 0.0 0.0 2.4387692109095793 0.0 48 0.0018218027102178147 0.0 0.0 2.582951882546818 0.0 49 0.0020300087342427077 0.0 0.0 2.7253127514738384 0.0 50 0.0020300087342427077 0.0 0.0 2.8681941354609215 0.0 51 0.002082060240248931 0.0 0.0 3.018310678782869 0.0 52 0.002082060240248931 0.0 0.0 3.1746213513195576 0.0 53 0.002082060240248931 0.0 0.0 3.3466515786701256 0.0 54 0.002082060240248931 0.0 0.0 3.5342972578225607 0.0 55 0.002082060240248931 0.0 0.0 3.7282932207077546 0.0 56 0.002446420782292494 0.0 0.0 3.939153871538965 0.0 57 0.002446420782292494 0.0 0.0 4.158550969355196 0.0 58 0.002446420782292494 0.0 0.0 4.388098110842641 0.0 59 0.002446420782292494 0.0 0.0 4.607339054140853 0.0 60 0.002446420782292494 0.0 0.0 4.830171551353495 0.0 61 0.0027066783123236107 0.0 0.0 5.058105096154747 0.0 62 0.0027066783123236107 0.0 0.0 5.290410967460522 0.0 63 0.0027066783123236107 0.0 0.0 5.54140332942253 0.0 64 0.0027066783123236107 0.0 0.0 5.798902129635317 0.0 65 0.0027066783123236107 0.0 0.0 6.075764090082418 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGCGC 255 0.0 30.625193 36 ATTCGCG 295 0.0 27.677366 56 CGGTTAC 260 0.0 27.304258 4 CCTCGCG 315 0.0 27.044218 35 CGATCGT 295 0.0 26.469868 1 AATTCGC 325 0.0 25.123188 55 TAGCGCG 85 1.1900405E-4 25.056324 5 CGCGGAA 315 0.0 24.794407 59 AACTCGA 365 0.0 24.313274 13 CGCGCTT 325 0.0 24.029625 38 ACTCGAG 355 0.0 23.998232 14 TCGCGCT 330 0.0 23.665537 37 CGCTCCG 370 0.0 23.023531 25 TCGCGGA 355 0.0 23.0007 58 CAATTCG 360 0.0 22.680065 54 GATCGTT 385 0.0 21.204573 2 ATCGTTC 420 0.0 21.127745 3 CTCGAGG 415 0.0 19.673761 15 CGGTATA 110 6.797399E-4 19.364225 10 GCGTCGT 110 6.805742E-4 19.360697 3 >>END_MODULE