FastQCFastQC Report
Thu 26 May 2016
SRR936028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1921174
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148450.7727046066623846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103300.5376920570442865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77650.40417994413832375No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23190.12070744242843177No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19560.10181274574817274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14750.052.868847100-101
CGTACAT17500.047.792404100-101
CGGTATC13050.046.75563100-101
GGTATCA104800.041.452091
AGTATCA31200.040.73464100-101
TACGCAG11300.040.036465100-101
CCGTATC6800.039.83117100-101
TCGTACA8700.039.68527100-101
AGGTATC22050.039.550285100-101
CCCGGTA3500.039.1168198-99
ACGTATC4750.038.22318100-101
TCCGTAC2900.036.9469198-99
GGGTATC12200.036.83899100-101
CCGTACA8000.036.83289100-101
TCGAGTA4050.036.74562100-101
TTTACGC3200.036.273498-99
CGAGTAC11350.036.18877100-101
GCGTATC4050.036.010708100-101
TCGTATC4200.034.724613100-101
ATATCAA22200.034.590538100-101
Produced by FastQC (version 0.11.5)