FastQCFastQC Report
Thu 26 May 2016
SRR936027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576255
Sequences flagged as poor quality0
Sequence length77
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429647.455727065274921No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA325765.653052901927098No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG8820.15305724028424916No Hit
GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC6770.11748271164675361No Hit
TACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTA6710.11644150593053423No Hit
ATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGA6670.11574736878638799No Hit
ATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTC6610.11470616307016859No Hit
TACAAAATGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTG6550.11366495735394921No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6220.10793832591474262No Hit
AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG5850.10151755733138976No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT200.00657259853.24700526
CGGTATA1100.048.40637210
GTCCGAG305.479244E-447.3347841
ATGGCGG1100.045.1753586
GAGTCCG350.001170998440.56914539
GCGGTAT1500.037.8645369
GTGGGTA1500.037.861251
ATAGGTG1051.0913936E-1137.1883814
TATAGGT1051.0913936E-1137.1883813
GATGGCG1350.036.8095475
AGTCCGA400.002255529635.50108340
CGATTGC552.7452424E-432.28772768
AGATGGC1550.032.059934
ACGTATA450.00401897531.553782
GCTGAAT1800.029.58423620
GGTATAT1800.029.58166911
GCAAGAG1950.029.12403929
GAACGAT755.0678223E-528.40086765
CGTATAG500.00672988728.3959373
GACGTAT500.00672988728.3959371