##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936027_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 576255 Sequences flagged as poor quality 0 Sequence length 77 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.01662979063089 40.0 36.0 41.0 31.0 41.0 2 36.80806413827212 40.0 36.0 41.0 31.0 41.0 3 36.69511067149092 39.0 35.0 41.0 31.0 41.0 4 36.53525435787976 39.0 35.0 41.0 31.0 41.0 5 36.37281585409237 39.0 35.0 41.0 30.0 41.0 6 36.08857189959306 39.0 35.0 40.0 30.0 41.0 7 35.69636532437897 38.0 35.0 40.0 30.0 41.0 8 35.282086923323874 37.0 35.0 40.0 30.0 41.0 9 35.00967800713226 37.0 35.0 40.0 29.0 41.0 10-11 34.96846534954143 37.0 35.0 40.0 28.5 41.0 12-13 34.73990420907411 38.0 35.0 40.0 26.5 41.0 14-15 34.44500004338357 37.0 35.0 40.0 24.5 41.0 16-17 34.0411172137335 37.0 34.0 40.0 22.0 41.0 18-19 33.53791030012755 37.0 33.0 40.0 18.0 41.0 20-21 32.818032815333495 36.5 33.0 40.0 5.5 41.0 22-23 31.90368847124971 36.0 32.5 40.0 2.0 41.0 24-25 31.222260978212773 36.0 31.0 40.0 2.0 41.0 26-27 30.182876504325343 35.0 29.0 39.0 2.0 40.5 28-29 30.17170263164745 35.0 29.5 39.5 2.0 40.5 30-31 30.454795186158904 35.0 30.5 40.0 2.0 41.0 32-33 30.272889606163936 35.0 30.0 40.0 2.0 41.0 34-35 30.002952685876913 35.0 29.5 40.0 2.0 41.0 36-37 29.722512602927523 35.0 28.5 39.5 2.0 41.0 38-39 29.42230262644142 35.0 28.0 39.0 2.0 41.0 40-41 29.0396395692879 35.0 27.0 39.0 2.0 41.0 42-43 28.678101708445045 35.0 26.5 38.5 2.0 40.5 44-45 28.287778847905876 35.0 26.0 38.0 2.0 40.0 46-47 27.908002533600573 35.0 25.5 37.0 2.0 40.0 48-49 27.442642580107766 35.0 24.0 37.0 2.0 39.5 50-51 26.98653721008928 34.5 21.5 36.0 2.0 39.0 52-53 26.53776626667014 34.0 19.0 36.0 2.0 39.0 54-55 26.049708896235174 34.0 11.5 35.0 2.0 37.5 56-57 25.54238401402157 34.0 2.0 35.0 2.0 37.0 58-59 25.050794353194334 33.0 2.0 35.0 2.0 36.5 60-61 24.615198132771084 33.0 2.0 35.0 2.0 36.0 62-63 24.19325125161604 33.0 2.0 35.0 2.0 36.0 64-65 23.734960217264927 33.0 2.0 35.0 2.0 35.5 66-67 23.25462772557288 32.5 2.0 35.0 2.0 35.0 68-69 22.804924903037715 32.0 2.0 35.0 2.0 35.0 70-71 22.316414608116197 32.0 2.0 35.0 2.0 35.0 72-73 21.798044268596367 31.0 2.0 35.0 2.0 35.0 74-75 21.26804192588351 31.0 2.0 35.0 2.0 35.0 76-77 20.076195434312936 28.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8908963511454502 1101 2 0.8133815420428192 1101 3 0.9216988149566561 1101 4 0.9988214164794087 1101 5 1.1096099278441187 1101 6 0.9401523990680403 1101 7 0.8139443730532676 1101 8 1.1560429936442063 1101 9 1.263368759776391 1101 10-11 1.2084762180096291 1101 12-13 1.3201770219300215 1101 14-15 1.1572140235377901 1101 16-17 1.4043043664091996 1101 18-19 1.7189440101701194 1101 20-21 1.657564939500837 1101 22-23 1.6273072344153654 1101 24-25 1.495819340954526 1101 26-27 1.2809138717332438 1101 28-29 1.0929365008454432 1101 30-31 1.1111229019131699 1101 32-33 1.0454015834748347 1101 34-35 1.0531427405591351 1101 36-37 0.9916135191628328 1101 38-39 0.9992784921164848 1101 40-41 1.0503915483223984 1101 42-43 0.7818858097538488 1101 44-45 0.571925234298515 1101 46-47 0.378961563153684 1101 48-49 0.6654171958096029 1101 50-51 0.44957729484716324 1101 52-53 0.573865671176133 1101 54-55 0.5673235683154907 1101 56-57 0.3828016621928363 1101 58-59 0.739477749845129 1101 60-61 0.7188438324398767 1101 62-63 0.7304168717648984 1101 64-65 0.5639361620356098 1101 66-67 0.6441524904192484 1101 68-69 0.5184681526217787 1101 70-71 0.5562017716670695 1101 72-73 0.3732919731599935 1101 74-75 0.46829006999602285 1101 76-77 0.5014359218464968 1102 1 0.6571075373537028 1102 2 0.7141553690581972 1102 3 0.5094890000874273 1102 4 0.9857936958407549 1102 5 0.8768006419199352 1102 6 0.8541253640988913 1102 7 0.7540952203448725 1102 8 0.7071498601345638 1102 9 0.6884838543008271 1102 10-11 0.5838802732549979 1102 12-13 1.013192988234266 1102 14-15 0.9544884872462802 1102 16-17 0.9588640686327281 1102 18-19 1.2752178861618901 1102 20-21 1.2500813465926228 1102 22-23 1.2954554369817295 1102 24-25 1.051542232520788 1102 26-27 1.1020275949186669 1102 28-29 0.9480520851338206 1102 30-31 1.0899161235803234 1102 32-33 1.1996404903217375 1102 34-35 1.2125609010057978 1102 36-37 1.1934574803735245 1102 38-39 1.5501404766399425 1102 40-41 1.427632218319463 1102 42-43 1.3861565512564447 1102 44-45 1.4911264293225095 1102 46-47 1.498696600865511 1102 48-49 1.4619570136174218 1102 50-51 1.534722754735494 1102 52-53 1.1745121595687245 1102 54-55 1.2430263795604723 1102 56-57 1.3367516083188065 1102 58-59 1.264859765713478 1102 60-61 1.3839922309118116 1102 62-63 1.3468215147269937 1102 64-65 1.5144944929201252 1102 66-67 1.676170807590374 1102 68-69 1.7945113498007395 1102 70-71 1.6915480347682532 1102 72-73 1.794844035066152 1102 74-75 1.6316140978146123 1102 76-77 1.648377346080018 1103 1 0.021896542386606654 1103 2 -0.19292941607536562 1103 3 -0.3108265244963917 1103 4 -0.07973088797650973 1103 5 -0.03812386446798399 1103 6 -0.12071009854144421 1103 7 -0.2169171683504416 1103 8 0.12004184619728164 1103 9 0.1380579928993555 1103 10-11 0.21403416407962794 1103 12-13 0.12679635641080012 1103 14-15 0.08229530102870797 1103 16-17 0.411559577730678 1103 18-19 0.3604385597971955 1103 20-21 0.17737035162553383 1103 22-23 -0.050726328407353805 1103 24-25 -0.09528316430461103 1103 26-27 -0.1357288897890392 1103 28-29 0.2105617638020334 1103 30-31 0.4939279315555467 1103 32-33 0.26983503155286215 1103 34-35 0.41140913948818536 1103 36-37 0.49199600147302647 1103 38-39 0.6024052850023125 1103 40-41 0.5720137514205064 1103 42-43 0.6166056414616286 1103 44-45 0.7579431153465173 1103 46-47 0.5081091057048432 1103 48-49 0.5088743577908623 1103 50-51 0.5212210125752144 1103 52-53 0.5797821944119299 1103 54-55 0.5862803478144372 1103 56-57 0.6906521116095554 1103 58-59 0.7613270518149129 1103 60-61 0.5070323005982225 1103 62-63 0.4144028155400008 1103 64-65 0.5933514422039003 1103 66-67 0.7785711173077559 1103 68-69 0.6974101109312052 1103 70-71 1.0302288322905184 1103 72-73 0.9439197222515929 1103 74-75 1.2691721698239036 1103 76-77 1.1695010394598064 1104 1 0.15216580031543714 1104 2 0.054698842191193364 1104 3 -0.2084309885902158 1104 4 -0.1682375349937999 1104 5 -0.12820399710130914 1104 6 0.024994669503747957 1104 7 0.06877025297527695 1104 8 0.3862986390498193 1104 9 0.31012056389810283 1104 10-11 -0.14719016124044515 1104 12-13 0.036710467122787804 1104 14-15 0.001533273458171891 1104 16-17 0.13527661128814117 1104 18-19 -0.16388906293467898 1104 20-21 -0.11543421378542007 1104 22-23 0.23696248986656698 1104 24-25 0.29066215515134886 1104 26-27 0.19475298058645052 1104 28-29 -0.10149305490172011 1104 30-31 0.2670677645358026 1104 32-33 0.7145423632282792 1104 34-35 0.39655313261090086 1104 36-37 0.31033133044665107 1104 38-39 0.28829917834301355 1104 40-41 0.24770109599083057 1104 42-43 0.543096998804053 1104 44-45 0.4970726324015651 1104 46-47 0.16594314518781772 1104 48-49 0.5698162232540014 1104 50-51 0.3852833686588504 1104 52-53 0.7047994643760127 1104 54-55 0.5188161925843282 1104 56-57 0.4406597871491442 1104 58-59 0.31998352536500363 1104 60-61 0.17877058004926383 1104 62-63 0.12914330554644593 1104 64-65 0.29513562138169647 1104 66-67 0.3911520942504332 1104 68-69 0.3542348169580407 1104 70-71 0.07789201426220771 1104 72-73 0.16530722954335886 1104 74-75 0.5718513169179431 1104 76-77 0.6637156014935179 1105 1 0.391999794043258 1105 2 0.7670798303416504 1105 3 0.432612372097843 1105 4 0.5149909489783369 1105 5 0.5061202409411649 1105 6 0.11119774550231654 1105 7 0.4778855747532731 1105 8 0.8125291323738821 1105 9 0.6689795524616926 1105 10-11 0.7816543675007992 1105 12-13 1.125553869124623 1105 14-15 0.8687338894090431 1105 16-17 0.8004735089989765 1105 18-19 0.9059424895244277 1105 20-21 0.800345771566974 1105 22-23 0.36795721297036366 1105 24-25 0.32702766941399375 1105 26-27 0.4122099297517252 1105 28-29 0.5545265503639065 1105 30-31 0.7741887575688011 1105 32-33 0.8546073385339064 1105 34-35 0.8781027748711416 1105 36-37 1.0962339426041687 1105 38-39 1.0933206802866415 1105 40-41 1.3870449580779258 1105 42-43 1.0103257832400239 1105 44-45 0.7917638119099273 1105 46-47 0.732694943276119 1105 48-49 0.5946213912320673 1105 50-51 0.7872288720296758 1105 52-53 0.6985235258959825 1105 54-55 0.3651946643501134 1105 56-57 0.5653785717729072 1105 58-59 0.5986055230739673 1105 60-61 0.3706881027248947 1105 62-63 0.3561099352811006 1105 64-65 0.43757050523364427 1105 66-67 0.4544110001864752 1105 68-69 0.274835369060348 1105 70-71 0.3751221132274871 1105 72-73 0.4667025085052572 1105 74-75 0.3203799830462799 1105 76-77 0.027320040846770866 1106 1 -0.23081292308881274 1106 2 0.19976654045037634 1106 3 -0.2858004276617834 1106 4 0.06580501819769324 1106 5 -0.23845544777829275 1106 6 -0.222587535525264 1106 7 -0.2871291667539282 1106 8 -0.5808193493447646 1106 9 -0.6879452001251138 1106 10-11 -0.7690470277781642 1106 12-13 -0.6615487204981036 1106 14-15 -0.46945318688999293 1106 16-17 -0.570719520239912 1106 18-19 -0.6339084052944486 1106 20-21 -0.7624361480214006 1106 22-23 -0.5802522103268508 1106 24-25 -0.8244248854676073 1106 26-27 -0.8371619130305703 1106 28-29 -0.6498489543214525 1106 30-31 -0.628483492717578 1106 32-33 -0.6732719501179503 1106 34-35 -0.5541238499809751 1106 36-37 -0.14614836007559262 1106 38-39 -0.025047049896830487 1106 40-41 0.08425815595214559 1106 42-43 0.10209119609612216 1106 44-45 0.11892949893928062 1106 46-47 -0.05069128421256508 1106 48-49 -0.1671276839026703 1106 50-51 -0.19982301431987182 1106 52-53 -0.0853359321423639 1106 54-55 0.0400927883290052 1106 56-57 0.11183967109498383 1106 58-59 -0.03978436631778237 1106 60-61 0.025965937882922674 1106 62-63 -0.012055920759163286 1106 64-65 0.1326597993314067 1106 66-67 0.13970141726784746 1106 68-69 0.05539535854411426 1106 70-71 0.104971317937256 1106 72-73 -0.11902545904465 1106 74-75 -0.0993285670278965 1106 76-77 0.48093030168694284 1107 1 0.5082217679204675 1107 2 0.3665943934484446 1107 3 0.40455831049276014 1107 4 0.40942064793761546 1107 5 0.4631608220685024 1107 6 0.4985659092237711 1107 7 0.06869719729989754 1107 8 0.3028099174545602 1107 9 0.20069359870043257 1107 10-11 0.01167462314094081 1107 12-13 -0.11024972101297692 1107 14-15 -0.07960375115811047 1107 16-17 0.08628408384007713 1107 18-19 0.4003494881973282 1107 20-21 0.06312603279487661 1107 22-23 -0.016660723748223916 1107 24-25 -0.014105249493599814 1107 26-27 -0.1448358899032307 1107 28-29 -0.0670090367004228 1107 30-31 0.12585810170514122 1107 32-33 0.26831481915135313 1107 34-35 0.09356341606692453 1107 36-37 0.1373511459288892 1107 38-39 -0.06409009012615385 1107 40-41 -0.04431461445821938 1107 42-43 0.07071378351941249 1107 44-45 0.2372953478462314 1107 46-47 0.2246572957242563 1107 48-49 0.4091041739379442 1107 50-51 0.29258963196542 1107 52-53 0.324450745285521 1107 54-55 0.38481295030387486 1107 56-57 0.36064468897623314 1107 58-59 0.5253586836522999 1107 60-61 0.6179221601391625 1107 62-63 0.9208476596646982 1107 64-65 0.48056310144793457 1107 66-67 0.5687104344254905 1107 68-69 0.49563581608137497 1107 70-71 0.4422206673938618 1107 72-73 0.5488286522504531 1107 74-75 0.2987413716053382 1107 76-77 0.41686743206001253 1108 1 0.003646818254530615 1108 2 -0.18643590958185996 1108 3 -0.20490385239725128 1108 4 -0.17711226476965436 1108 5 -0.30626748555278027 1108 6 -0.3398553323925597 1108 7 -0.58234667348583 1108 8 -0.02937246498174062 1108 9 -0.3349281712632788 1108 10-11 -0.5185065794905341 1108 12-13 -0.36007339148835626 1108 14-15 -0.4055665103625117 1108 16-17 -0.22868212501102647 1108 18-19 -0.26494341117300735 1108 20-21 0.0070484349800850055 1108 22-23 -0.36672772896757877 1108 24-25 -0.37604243771094303 1108 26-27 -0.1836556362451951 1108 28-29 -0.28099773878687984 1108 30-31 -0.4302243050966936 1108 32-33 -0.3325809348336932 1108 34-35 -0.29499311838466014 1108 36-37 -0.22670359957951547 1108 38-39 -0.24181422098189742 1108 40-41 -0.21533982637424742 1108 42-43 0.13364579085472172 1108 44-45 0.1274628929545294 1108 46-47 0.11817510180025081 1108 48-49 0.0938045419857545 1108 50-51 -0.0024930184917550946 1108 52-53 -0.13101803477065133 1108 54-55 0.052741174569383986 1108 56-57 -0.13164650480671014 1108 58-59 -0.007622952429212404 1108 60-61 0.07016891888190102 1108 62-63 -0.12135921654556014 1108 64-65 -0.18042094553907262 1108 66-67 -0.19480762812981212 1108 68-69 -0.19372285740764283 1108 70-71 0.15522352713815124 1108 72-73 0.11039009828079571 1108 74-75 -0.028558285994783716 1108 76-77 -0.24918689025753338 1109 1 -0.3573561871383575 1109 2 -0.5766905430306863 1109 3 -0.5865337523361092 1109 4 -0.6558335008304894 1109 5 -0.6354793220775292 1109 6 -0.5225696324336511 1109 7 -0.42815287255347556 1109 8 -0.34445992547327364 1109 9 0.049121571385803975 1109 10-11 -0.3505183810763697 1109 12-13 -0.3050753487140838 1109 14-15 -0.23592841919138152 1109 16-17 -0.083051590995737 1109 18-19 0.0033065055235823593 1109 20-21 0.05670050171339369 1109 22-23 -0.07131119574811962 1109 24-25 0.059482341413708895 1109 26-27 -0.12436787230279478 1109 28-29 0.043785267964739916 1109 30-31 0.33087122369393995 1109 32-33 0.36650450696552994 1109 34-35 0.6525111761891331 1109 36-37 0.6096464494320024 1109 38-39 0.2012515385672593 1109 40-41 0.48371649967044306 1109 42-43 0.3995615945627726 1109 44-45 0.19667104148369674 1109 46-47 0.06809923908426896 1109 48-49 0.294554434419215 1109 50-51 0.4605412960425035 1109 52-53 0.38618937615940396 1109 54-55 0.4715502755072336 1109 56-57 0.43900287357193335 1109 58-59 0.5843472459784813 1109 60-61 0.514091975647915 1109 62-63 0.6126408675666148 1109 64-65 0.7451947717922387 1109 66-67 0.8814756487513087 1109 68-69 0.8826235036083006 1109 70-71 0.6464046885979506 1109 72-73 0.6594711727168416 1109 74-75 0.5956834912308722 1109 76-77 0.5169699271963921 1110 1 0.029463910419089245 1110 2 -0.2787544437608602 1110 3 -0.4332425451682269 1110 4 -0.44176484884768286 1110 5 -0.4517451269264612 1110 6 -0.47462298152409943 1110 7 -0.6970297560211662 1110 8 -0.4610766477119128 1110 9 -0.4083613500316119 1110 10-11 -0.6377792356988934 1110 12-13 -0.7075751829026018 1110 14-15 -0.6963130415331165 1110 16-17 -1.0416288681903225 1110 18-19 -0.9303975420854158 1110 20-21 -1.2311060234206117 1110 22-23 -1.0813880116853163 1110 24-25 -1.3882418206353577 1110 26-27 -1.537122392926726 1110 28-29 -1.2898304473459916 1110 30-31 -1.562435117540062 1110 32-33 -1.2367402729875607 1110 34-35 -1.1074631381050217 1110 36-37 -1.2863354861607874 1110 38-39 -1.3095548478880552 1110 40-41 -1.4456357502406227 1110 42-43 -1.4477169868144877 1110 44-45 -1.4182981175807647 1110 46-47 -1.34739569311391 1110 48-49 -1.3249896141545037 1110 50-51 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2313 66-67 -1.2702948490110408 2313 68-69 -1.3763870300829169 2313 70-71 -1.3804840135638372 2313 72-73 -1.2915823281089445 2313 74-75 -1.2537432009688274 2313 76-77 -1.1803201299368347 2314 1 -0.37013056454021154 2314 2 -0.4980187870673163 2314 3 -0.2169244459954598 2314 4 -0.14971542429342577 2314 5 0.0318720422041352 2314 6 -0.04192490729207066 2314 7 -0.09504401603316381 2314 8 0.1992818516550514 2314 9 0.1360226993167899 2314 10-11 -0.06176232930283021 2314 12-13 -0.28674061998434297 2314 14-15 -0.28686602157100793 2314 16-17 -0.5390277011159128 2314 18-19 -0.34250522516664006 2314 20-21 -0.36359419560692885 2314 22-23 -0.4411701133711965 2314 24-25 -0.4273259623216177 2314 26-27 -0.2660274753168501 2314 28-29 -0.1827018692538651 2314 30-31 -0.3015449615176351 2314 32-33 -0.38514006736922823 2314 34-35 -0.4499009528390978 2314 36-37 -0.5352359992546774 2314 38-39 -0.4144796924843881 2314 40-41 -0.31529181550540386 2314 42-43 -0.32347258658221634 2314 44-45 -0.5205765781087095 2314 46-47 -0.48181391699038656 2314 48-49 -0.3970274250808359 2314 50-51 -0.510382889730689 2314 52-53 -0.6397480962143476 2314 54-55 -0.7022935822351357 2314 56-57 -0.5540834139282644 2314 58-59 -0.5132897672145127 2314 60-61 -0.3567536865760168 2314 62-63 -0.4780876333616213 2314 64-65 -0.5222481166263186 2314 66-67 -0.6000219149385693 2314 68-69 -0.6302067763203496 2314 70-71 -0.6830705036807387 2314 72-73 -0.49555303437732334 2314 74-75 -0.6950538500969579 2314 76-77 -0.4479518872696211 2315 1 -0.6015110466862197 2315 2 -0.558655964205343 2315 3 -0.44519364759742075 2315 4 -0.21044483306813788 2315 5 -0.21204579375471866 2315 6 -0.35827737151644357 2315 7 -0.6024594396223293 2315 8 -0.5082536780209921 2315 9 -0.5066555434450777 2315 10-11 -0.6018380002367465 2315 12-13 -0.5636415140163393 2315 14-15 -0.7436124583554431 2315 16-17 -0.6872593132019418 2315 18-19 -0.4659716180078348 2315 20-21 -0.6941595550438606 2315 22-23 -0.6765821428361285 2315 24-25 -1.0144448919427305 2315 26-27 -0.7217275954229372 2315 28-29 -0.8087302520028565 2315 30-31 -1.0758869400297115 2315 32-33 -1.0095564460682276 2315 34-35 -0.7498331113463408 2315 36-37 -0.9090190540484713 2315 38-39 -0.5598162559745923 2315 40-41 -0.7070200724094526 2315 42-43 -0.6831933911700183 2315 44-45 -0.6684525859955386 2315 46-47 -0.4382385678424008 2315 48-49 -0.38296517487485104 2315 50-51 -0.2537381127057934 2315 52-53 -0.413767263998043 2315 54-55 -0.24803227566127362 2315 56-57 -0.42550021484320055 2315 58-59 -0.2903454054369412 2315 60-61 -0.18062097959797896 2315 62-63 -0.07012865799361734 2315 64-65 -0.0663963267176868 2315 66-67 -0.19200760058855693 2315 68-69 0.01740380941203057 2315 70-71 0.17132751067460106 2315 72-73 -0.8917596496402211 2315 74-75 -1.1488867919582333 2315 76-77 -1.0842415519973763 2316 1 -2.1351176146275535 2316 2 -1.9870092101551577 2316 3 -1.69151078018065 2316 4 -2.0259985842442063 2316 5 -2.576304902649021 2316 6 -2.365991093822444 2316 7 -2.677824504257778 2316 8 -2.4191044429490134 2316 9 -2.238711633870267 2316 10-11 -2.1001818376363772 2316 12-13 -2.269144227029784 2316 14-15 -2.3101328413994366 2316 16-17 -3.5846062309351296 2316 18-19 -3.4428111184524823 2316 20-21 -2.5864380996829226 2316 22-23 -2.7194039345849568 2316 24-25 -2.663547857569302 2316 26-27 -2.2748859039460534 2316 28-29 -2.242757627017358 2316 30-31 -2.432954307826691 2316 32-33 -2.667504093286265 2316 34-35 -2.7631404100613643 2316 36-37 -2.7052369457599212 2316 38-39 -2.772755590158564 2316 40-41 -2.956166214259458 2316 42-43 -3.146693371837383 2316 44-45 -3.0255457306423317 2316 46-47 -2.7260983160025773 2316 48-49 -2.761208224791723 2316 50-51 -3.232800866446663 2316 52-53 -4.015482713352977 2316 54-55 -3.3065231699890774 2316 56-57 -3.2089617880043484 2316 58-59 -3.3178235273356655 2316 60-61 -2.951703220637299 2316 62-63 -3.006956043318862 2316 64-65 -2.944910033557573 2316 66-67 -2.8259441122074698 2316 68-69 -2.735692634671537 2316 70-71 -2.5587083191466355 2316 72-73 -2.5085416677097925 2316 74-75 -2.470826757675024 2316 76-77 -2.3150281325693634 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13565.0 3 2236.0 4 4284.0 5 5226.0 6 5594.0 7 5480.0 8 7412.0 9 8568.0 10 13238.0 11 15496.0 12 9592.0 13 5422.0 14 4557.0 15 4270.0 16 4508.0 17 4523.0 18 4850.0 19 5038.0 20 5110.0 21 5572.0 22 5937.0 23 6394.0 24 7407.0 25 8064.0 26 9112.0 27 10252.0 28 11510.0 29 12850.0 30 14404.0 31 17190.0 32 20618.0 33 25848.0 34 43168.0 35 52344.0 36 47291.0 37 64189.0 38 65054.0 39 19965.0 40 117.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.716095664549274 29.094033114651673 40.62150716789892 14.568364052900135 2 15.350302441225733 29.643058605038664 40.77202787492732 14.234611078808287 3 14.942957662140655 30.16587665386331 40.00982381354888 14.881341870447157 4 15.549345645155691 28.667337799840315 41.11917242336932 14.664144131634671 5 14.904791575907261 29.926044463575032 40.74497055490083 14.424193405616872 6 15.01857948914649 30.290207975729512 40.47968231763724 14.211530217486763 7 15.377098462862934 30.34977852451505 40.379875584931334 13.893247427690683 8 14.5593404209156 30.695942720763725 40.110045563028855 14.63467129529182 9 14.920494960312569 30.921133708366384 39.71294523603303 14.445426095288019 10-11 14.722734760262382 30.676312989382215 39.97186296343227 14.629089286923133 12-13 14.912338558363722 30.965725166062935 38.87868684010921 15.243249435464135 14-15 14.629202172894356 31.711501414463978 38.88595775698988 14.773338655651783 16-17 14.382505486342934 31.83354491945988 38.87775759779314 14.906191996404047 18-19 14.546655349756737 31.87064008234267 38.04052564616605 15.542178921734543 20-21 14.894867163856556 31.787896119540815 37.41578133828151 15.901455378321119 22-23 15.203992189195054 32.4598828379258 36.21714037752224 16.11898459535691 24-25 15.095485847439608 33.49142775316442 34.581888714835564 16.831197684560408 26-27 15.594794329134137 33.59115782347759 33.89605148389393 16.917996363494346 28-29 15.235348890487549 34.23030963602299 33.53573080471002 16.998610668779445 30-31 15.653360130375246 34.115550094327666 32.94977463757174 17.281315137725343 32-33 16.060708093313224 34.342560403494126 32.313895103329415 17.282836399863232 34-35 15.604400181894551 33.5571145414973 33.40116773523929 17.43731754136886 36-37 16.25289630574575 34.514592174116785 32.48366353388352 16.74884798625394 38-39 16.583296160848956 34.50046255877447 32.73102337440531 16.18521790597126 40-41 16.620498855350693 34.03010280148362 33.27735920119272 16.07203914197296 42-43 16.48080521256765 34.433199223807854 32.40541459977159 16.68058096385291 44-45 16.644956934892498 34.05757924196733 32.850280953723995 16.44718286941618 46-47 16.331653057823697 34.16418444148198 33.10220865966783 16.401953841026497 48-49 16.21661498297606 34.0945720408158 32.75261868371925 16.9361942924889 50-51 15.685302715727053 34.79876816676272 32.68611167047419 16.82981744703603 52-53 15.075141337587855 34.674127127008084 33.46565060428166 16.785080931122394 54-55 14.938318646056741 34.327780434690155 33.40844053323798 17.32546038601513 56-57 15.001861628495327 34.86010013738732 32.76162389549885 17.376414338618506 58-59 15.128737858685163 34.236598557275066 32.45046274117737 18.1842008428624 60-61 15.132203036785988 33.526759561015616 32.68524264345321 18.655794758745188 62-63 15.048969233226172 34.095674876231605 32.41950714836237 18.43584874217985 64-65 15.196266484284275 33.389170582506914 33.030105245981886 18.384457687226927 66-67 15.14862983498304 33.39492971242679 32.946887661397554 18.509552791192615 68-69 15.502110246719178 33.56837425064974 32.4850390365261 18.444476466104977 70-71 15.27329961471882 32.880337116693845 33.20424069743012 18.642122571157216 72-73 16.038619822445384 32.55199807560877 32.69433007998902 18.71505202195682 74-75 15.96976896864351 32.34074259334521 32.615593834995465 19.07389460301581 76-77 15.829334908097733 32.06952700619629 33.50887324738709 18.59226483831888 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 59928.0 1 34185.5 2 7333.5 3 5819.5 4 5415.0 5 5230.5 6 4745.5 7 4390.5 8 4214.0 9 4092.0 10 4001.0 11 3829.5 12 3853.0 13 3957.0 14 4070.0 15 4322.5 16 4685.5 17 4909.0 18 5113.5 19 5610.0 20 6061.5 21 6704.5 22 7188.0 23 7220.0 24 7433.5 25 8004.5 26 8394.0 27 9029.0 28 10455.5 29 12462.5 30 13678.0 31 15208.5 32 17909.0 33 20220.0 34 21766.0 35 22171.0 36 24499.5 37 28078.0 38 27481.5 39 25635.0 40 24935.0 41 22760.5 42 20270.0 43 19254.0 44 17896.0 45 15919.5 46 14105.5 47 12752.0 48 12594.0 49 11958.5 50 11375.0 51 9882.5 52 8338.0 53 7704.0 54 6575.5 55 5914.0 56 5651.0 57 5555.0 58 6178.0 59 6226.5 60 5556.5 61 5461.0 62 5100.0 63 3541.0 64 1794.0 65 1245.0 66 1134.5 67 927.5 68 789.5 69 735.0 70 722.0 71 635.0 72 482.5 73 391.5 74 366.0 75 353.5 76 253.0 77 135.5 78 106.0 79 97.5 80 87.0 81 68.5 82 39.0 83 26.0 84 24.5 85 20.5 86 14.5 87 11.0 88 11.0 89 10.5 90 7.0 91 4.0 92 4.0 93 3.5 94 3.5 95 3.5 96 3.0 97 5.0 98 5.0 99 9.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013015071452742276 2 0.019088771464022002 3 0.01804756574780262 4 0.019956442894204823 5 0.017006360031583238 6 0.014229811454998222 7 0.020650580038351077 8 0.022559457184753277 9 0.02221238861268015 10-11 0.025336005761338296 12-13 0.020477045752314512 14-15 0.011279728592376638 16-17 0.008937015730883029 18-19 0.021865320040607022 20-21 0.026116910048502834 22-23 0.022559457184753277 24-25 0.02125795003947905 26-27 0.02646397862057596 28-29 0.01362244145387025 30-31 0.012494468594632584 32-33 0.015271017171217604 34-35 0.016832825745546676 36-37 0.01561808574329073 38-39 0.01943584003609513 40-41 0.01665929145951011 42-43 0.01891523717798544 44-45 0.016051921458382138 46-47 0.027591951479813626 48-49 0.02681104719264909 50-51 0.035227460065422427 52-53 0.041734995791793565 54-55 0.03288474720392882 56-57 0.02542277290435658 58-59 0.021344717182497332 60-61 0.02646397862057596 62-63 0.031062637200544894 64-65 0.030542034342435208 66-67 0.033492117205056786 68-69 0.0444247772253603 70-71 0.05414269724340787 72-73 0.08477149872886136 74-75 0.08485826587187964 76-77 0.08806865016355607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 576255.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.56067971143414 #Duplication Level Percentage of deduplicated Percentage of total 1 85.20880669287442 35.41335923556059 2 6.690784747638162 5.561471238294768 3 2.4734521595130845 3.0839505894923525 4 1.2307334155080107 2.0460046916835126 5 0.7696075192524966 1.5992705805582197 6 0.48607345544984526 1.2120925918908645 7 0.37782433573270985 1.0991845343200761 8 0.2947132875405858 0.9798787640142465 9 0.22953144675402926 0.8585534648021679 >10 1.8394220355238151 15.600163207014187 >50 0.23549859441604823 6.83993722407836 >100 0.15895109392021156 11.533929980043503 >500 0.0037646311717944845 1.039941656476958 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 8.365847048432188E-4 13.13226224177021 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42964 7.455727065274921 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32576 5.653052901927098 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 882 0.15305724028424916 No Hit GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC 677 0.11748271164675361 No Hit TACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTA 671 0.11644150593053423 No Hit ATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGA 667 0.11574736878638799 No Hit ATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTC 661 0.11470616307016859 No Hit TACAAAATGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTG 655 0.11366495735394921 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 622 0.10793832591474262 No Hit AGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCG 585 0.10151755733138976 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.4706857207312733E-4 0.0 2 0.0 0.0 0.0 0.004858960009023783 0.0 3 0.0 0.0 0.0 0.007114905727499111 0.0 4 0.0 0.0 0.0 0.01232093430859602 0.0 5 0.0 0.0 0.0 0.022038854326643587 0.0 6 0.0 0.0 0.0 0.038871680072190266 0.0 7 0.0 0.0 0.0 0.06524889154974794 0.0 8 0.0 0.0 0.0 0.0912790344552325 0.0 9 0.0 0.0 0.0 0.1160944373584611 0.0 10 0.0 0.0 0.0 0.14923948599144476 0.0 11 0.0 0.0 0.0 0.18307867176857467 0.0 12 0.0 0.0 0.0 0.22802405185204466 0.0 13 0.0 0.0 0.0 0.28511683195807414 0.0 14 0.0 0.0 0.0 0.3458538320708714 0.0 15 0.0 0.0 0.0 0.4192588350643378 0.0 16 0.0 0.0 0.0 0.5056789095105465 0.0 17 0.0 0.0 0.0 0.5768279667855376 0.0 18 0.0 0.0 0.0 0.6484976269186384 0.0 19 0.0 0.0 0.0 0.7196466841936295 0.0 20 0.0 0.0 0.0 0.8013813329168511 0.0 21 0.0 0.0 0.0 0.8808600359215972 0.0 22 0.0 0.0 0.0 0.9665859732236597 0.0 23 0.0 0.0 0.0 1.061162159113587 0.0 24 0.0 0.0 0.0 1.1475822335597956 0.0 25 0.0 0.0 0.0 1.2609001223416716 0.0 26 0.0 0.0 0.0 1.3702267225447067 0.0 27 0.0 0.0 0.0 1.4821563370382904 0.0 28 0.0 0.0 0.0 1.5826326886534607 0.0 29 0.0 0.0 0.0 1.6779030116875342 0.0 30 0.0 0.0 0.0 1.7856678033162403 0.0 31 0.0 0.0 0.0 1.8963826778075679 0.0 32 0.0 0.0 0.0 2.0138653894543213 1.7353428603656367E-4 33 0.0 0.0 0.0 2.128918621096563 1.7353428603656367E-4 34 0.0 0.0 0.0 2.2644488984911195 1.7353428603656367E-4 35 0.0 0.0 0.0 2.4126471787663446 1.7353428603656367E-4 36 0.0 0.0 0.0 2.564142610476265 1.7353428603656367E-4 37 0.0 0.0 0.0 2.7269177707785617 1.7353428603656367E-4 38 0.0 0.0 0.0 2.867480542468178 1.7353428603656367E-4 39 0.0 0.0 0.0 3.0172406313177325 1.7353428603656367E-4 40 0.0 0.0 0.0 3.15624159443302 1.7353428603656367E-4 41 0.0 0.0 0.0 3.3127695204380005 1.7353428603656367E-4 42 0.0 0.0 0.0 3.4734622693078583 1.7353428603656367E-4 43 0.0 0.0 0.0 3.6589704210809453 1.7353428603656367E-4 44 0.0 0.0 0.0 3.8406608185612274 1.7353428603656367E-4 45 0.0 0.0 0.0 4.042654727507787 1.7353428603656367E-4 46 0.0 0.0 0.0 4.256969570762943 1.7353428603656367E-4 47 0.0 0.0 0.0 4.4780522511735255 1.7353428603656367E-4 48 0.0 0.0 0.0 4.702085014446729 1.7353428603656367E-4 49 0.0 0.0 0.0 4.922647091999202 1.7353428603656367E-4 50 0.0 0.0 0.0 5.120996780938994 1.7353428603656367E-4 51 0.0 0.0 0.0 5.341558858491466 1.7353428603656367E-4 52 0.0 0.0 0.0 5.546676384586685 1.7353428603656367E-4 53 0.0 0.0 0.0 5.7845918907428135 1.7353428603656367E-4 54 0.0 0.0 0.0 6.0490581426625365 1.7353428603656367E-4 55 0.0 0.0 0.0 6.320292231737685 1.7353428603656367E-4 56 0.0 0.0 0.0 6.601764843688992 1.7353428603656367E-4 57 0.0 0.0 0.0 6.875254878482616 1.7353428603656367E-4 58 0.0 0.0 0.0 7.15585981900374 1.7353428603656367E-4 59 0.0 0.0 0.0 7.451562242410044 1.7353428603656367E-4 60 0.0 0.0 0.0 7.741885102949215 1.7353428603656367E-4 61 0.0 0.0 0.0 8.04470243208302 1.7353428603656367E-4 62 0.0 0.0 0.0 8.322010221169448 1.7353428603656367E-4 63 0.0 0.0 0.0 8.61424195885502 1.7353428603656367E-4 64 0.0 0.0 0.0 8.931809702301932 1.7353428603656367E-4 65 0.0 0.0 0.0 9.245386157170003 1.7353428603656367E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGAT 20 0.006572598 53.247005 26 CGGTATA 110 0.0 48.406372 10 GTCCGAG 30 5.479244E-4 47.33478 41 ATGGCGG 110 0.0 45.175358 6 GAGTCCG 35 0.0011709984 40.569145 39 GCGGTAT 150 0.0 37.864536 9 GTGGGTA 150 0.0 37.86125 1 ATAGGTG 105 1.0913936E-11 37.18838 14 TATAGGT 105 1.0913936E-11 37.18838 13 GATGGCG 135 0.0 36.809547 5 AGTCCGA 40 0.0022555296 35.501083 40 CGATTGC 55 2.7452424E-4 32.287727 68 AGATGGC 155 0.0 32.05993 4 ACGTATA 45 0.004018975 31.55378 2 GCTGAAT 180 0.0 29.584236 20 GGTATAT 180 0.0 29.581669 11 GCAAGAG 195 0.0 29.124039 29 GAACGAT 75 5.0678223E-5 28.400867 65 CGTATAG 50 0.006729887 28.395937 3 GACGTAT 50 0.006729887 28.395937 1 >>END_MODULE