FastQCFastQC Report
Thu 26 May 2016
SRR936026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576750
Sequences flagged as poor quality0
Sequence length77
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430787.469094061551799No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA328215.690680537494582No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG9130.15830082358040745No Hit
GACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTAC6980.12102297355873429No Hit
ATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGA6680.11582141309059385No Hit
ATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTGGTC6610.11460771564802774No Hit
TACTGTTGCTTGATTTAGTCGGCCTGGGATGGCATCAGTTTTAAGTCCTA6340.10992631122670135No Hit
TACAAAATGTCAGTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTG6130.10628521889900304No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCAT200.00656218953.2683548
TACCGAT200.006577967753.236021
ATCGCCA400.002254936135.5029953
TATAGGT1700.035.49683413
ATAGGTG1750.034.4856314
CGCTCCG552.7555847E-432.2670510
CGGTATA901.5530895E-731.55000510
CGCCAGG703.164124E-530.43113515
ATGGCGG952.4967085E-729.8868896
TAGTCCA604.5727217E-429.59609650
GACCTAC604.5866822E-429.58069443
CGTGGGC604.5890125E-429.5781324
GCCGTGG604.5890125E-429.5781322
CAGCGCT604.5913435E-429.5755657
GAACGAT657.2923105E-427.32658665
CCGCCAG657.3219545E-427.30762714
TGTTTCG657.325667E-427.30525842
ACGAAGC1304.645699E-927.30525842
TAGGTGG2250.026.82448215
TAACAGA2250.026.8151781