FastQCFastQC Report
Thu 26 May 2016
SRR936026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576750
Sequences flagged as poor quality0
Sequence length125
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201003.485045513654096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142202.4655396618985694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123862.1475509319462507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73951.282184655396619No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45310.7856090160381447No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43990.7627221499783269No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31940.553792804508019No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29700.5149544863459038No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25040.4341569137407889No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22290.38647594278283487No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.24534026874729084No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT9520.16506285218899003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9310.16142175986129173No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG8360.14495015171218031No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC8010.1388816644993498No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT7570.13125270914607717No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.13090593844820114No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG6630.11495448634590377No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG6630.11495448634590377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6570.11391417425227568No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6440.1116601647160815No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT6380.11061985262245341RNA PCR Primer, Index 27 (95% over 23bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.10784568703944516No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA5940.10299089726918075No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGT303.1445234E-759.52372798-99
ACGGGTA201.7684963E-459.5237298-99
ACGGTAC201.7684963E-459.5237298-99
GTAAGCG150.004231325459.51856296-97
CCGTACA2600.058.384098100-101
AACGTAT1400.057.39787798-99
CCCGGTA2400.057.0435798-99
CGTATCA11450.056.669422100-101
CACGGTA950.056.39089698-99
ACGTATC3200.055.80833100-101
TGTACAT17900.055.53812100-101
CCTATCG700.055.2672496-97
TTACGCA4650.055.048218100-101
TGAGTAC10350.054.92762100-101
CCCGTAC1800.054.5634198-99
AAGTATC18450.054.366486100-101
GCCCGTA553.6379788E-1254.11247698-99
ACCGTAC506.91216E-1153.5713598-99
CGTACTT2200.052.764236100-101
TCGAGTA1650.052.313267100-101
Produced by FastQC (version 0.11.5)