FastQCFastQC Report
Thu 26 May 2016
SRR936025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2904889
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87750.30207694682998215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGC3850.023.05140555
TCGCGCT4050.022.78723337
CGATCGT4400.022.5845431
CGCGGAA4000.022.18888959
CTCGCGC4200.021.97151236
CGGTTAC3400.020.8753914
CGCGCTT4500.020.5074538
CGCTCCG4800.019.96690625
AACTCGA5000.019.87747813
CAATTCG4900.019.56278654
ATTCGCG4700.018.88383556
CCTCGCG5300.018.08136735
TCGCGGA5400.017.09278158
ATCGTTC5400.017.0866013
GACGCTC5450.016.93567323
CTCCGCG6000.016.56513427
GCGGTTA5050.016.162653
ACTCGAG5750.016.05013714
CGTAACG1858.0248625E-515.34834832
GATCGTT6050.015.2516372
Produced by FastQC (version 0.11.5)