##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936025_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2904889 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.48884484054296 39.0 36.0 41.0 30.0 41.0 2 36.33606344338803 39.0 36.0 41.0 29.0 41.0 3 36.248794360128734 39.0 36.0 41.0 29.0 41.0 4 36.12489427306861 39.0 36.0 41.0 29.0 41.0 5 36.04791095287978 39.0 35.0 41.0 29.0 41.0 6 35.936517712036505 39.0 35.0 40.0 28.0 41.0 7 35.75319056941591 39.0 35.0 40.0 27.0 41.0 8 35.55971123165119 39.0 35.0 40.0 27.0 41.0 9 35.460072312573736 38.0 35.0 40.0 27.0 41.0 10-11 35.660553122683865 39.0 35.0 40.0 27.5 41.0 12-13 35.82102689638055 39.0 35.0 41.0 28.0 41.0 14-15 35.75057084797388 39.0 35.0 41.0 27.0 41.0 16-17 35.568925525209394 39.0 35.0 41.0 27.0 41.0 18-19 35.389009356295546 39.0 35.0 41.0 26.0 41.0 20-21 35.14753111048305 39.0 35.0 41.0 25.5 41.0 22-23 34.83744249780284 39.0 34.0 41.0 24.0 41.0 24-25 34.56456098666765 39.0 34.0 41.0 23.0 41.0 26-27 33.6086366811262 38.0 33.0 39.5 21.0 40.5 28-29 33.6256621165215 38.0 33.0 39.5 21.0 40.5 30-31 33.98337544050736 38.0 33.0 40.0 20.5 41.0 32-33 33.81271091597648 38.0 33.0 40.0 19.5 41.0 34-35 33.534752963021994 38.0 33.0 40.0 18.0 41.0 36-37 33.236370305371395 37.0 33.0 40.0 16.0 41.0 38-39 32.950977472805334 37.0 32.0 40.0 13.5 41.0 40-41 32.56761101715074 36.0 32.0 39.5 10.5 41.0 42-43 32.20003363295465 36.0 31.5 39.0 8.0 41.0 44-45 31.83368039191859 35.0 31.0 39.0 7.0 40.5 46-47 31.44042646724195 35.0 31.0 38.5 3.5 40.0 48-49 30.982993842449744 35.0 31.0 37.5 2.0 40.0 50-51 30.554346482774385 35.0 30.0 37.0 2.0 39.0 52-53 30.108824295868104 35.0 30.0 36.5 2.0 39.0 54-55 29.654308994250727 34.0 29.5 36.0 2.0 38.5 56-57 29.213132928659235 34.0 29.0 35.5 2.0 37.0 58-59 28.792685882317706 34.0 29.0 35.0 2.0 37.0 60-61 28.447001073018626 34.0 29.0 35.0 2.0 36.0 62-63 28.110041209836247 34.0 28.0 35.0 2.0 36.0 64-65 27.737432996579216 34.0 27.0 35.0 2.0 36.0 66-67 27.356196226430683 34.0 26.0 35.0 2.0 35.0 68-69 26.99695857569773 33.0 26.0 35.0 2.0 35.0 70-71 26.58312692842997 33.0 25.0 35.0 2.0 35.0 72-73 26.099423592433308 33.0 24.5 35.0 2.0 35.0 74-75 25.52030731638971 33.0 21.5 35.0 2.0 35.0 76-77 23.84638156569838 31.0 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.690088011717755 1101 2 0.6669962926991744 1101 3 0.6649768804910821 1101 4 0.6623064997986674 1101 5 0.7315457971604857 1101 6 0.7020520234625494 1101 7 0.701266729758153 1101 8 0.7478622468998353 1101 9 0.7814324237820571 1101 10-11 0.7232034154179914 1101 12-13 0.7880354114400987 1101 14-15 0.8656327711134395 1101 16-17 0.8583773424723233 1101 18-19 0.7686165528296911 1101 20-21 0.6636584029274673 1101 22-23 0.7444134495592891 1101 24-25 0.8325658203477815 1101 26-27 0.7874378465914518 1101 28-29 0.7508106254652276 1101 30-31 0.8666573863045741 1101 32-33 0.8951109393973979 1101 34-35 0.6962352248916375 1101 36-37 0.789186219900536 1101 38-39 0.7810733807943606 1101 40-41 0.6157460016575129 1101 42-43 0.6557533816579095 1101 44-45 0.6695582641943183 1101 46-47 0.6114242530272058 1101 48-49 0.7046478184753724 1101 50-51 0.6619137107332165 1101 52-53 0.815462041021366 1101 54-55 0.6696433767076684 1101 56-57 0.7119037469372742 1101 58-59 0.7873958540778965 1101 60-61 0.6297542980063042 1101 62-63 0.6661422057446593 1101 64-65 0.6572970720655213 1101 66-67 0.5657993173388576 1101 68-69 0.43402821922836665 1101 70-71 0.501378785728253 1101 72-73 0.37874461830695694 1101 74-75 0.3493867988552033 1101 76-77 0.39041766175278525 1102 1 0.6222793658091916 1102 2 0.7529088141157132 1102 3 0.6720029201665412 1102 4 0.7527069359172103 1102 5 0.9496185213438295 1102 6 0.8482919169816583 1102 7 0.5895566636323863 1102 8 0.7334115721904624 1102 9 0.6764623715327929 1102 10-11 0.526014114482031 1102 12-13 0.632549238380328 1102 14-15 0.6090265663359631 1102 16-17 0.7061238273285397 1102 18-19 0.6825344690892692 1102 20-21 0.7394662134141328 1102 22-23 0.7165542535466614 1102 24-25 0.6601011133826304 1102 26-27 0.49859072879696953 1102 28-29 0.5655112187563631 1102 30-31 0.7093690383159625 1102 32-33 0.6441360445331199 1102 34-35 0.6906899351135181 1102 36-37 0.7071725699564624 1102 38-39 0.7397543345912752 1102 40-41 0.6822608056165649 1102 42-43 0.7930298355043917 1102 44-45 0.7952439473287107 1102 46-47 0.638514931701291 1102 48-49 0.6779146720499867 1102 50-51 0.7715660194711624 1102 52-53 0.749990452445406 1102 54-55 0.8107730370166344 1102 56-57 0.7620825130616282 1102 58-59 0.7986836073594041 1102 60-61 0.6815203657179545 1102 62-63 0.6851551335443844 1102 64-65 0.6548789504903958 1102 66-67 0.7624859491726781 1102 68-69 0.6683923782134045 1102 70-71 0.8914071759127573 1102 72-73 0.8418256967091757 1102 74-75 0.8936184939120508 1102 76-77 1.0063282008122982 1103 1 0.7734934711517027 1103 2 0.5631548100471306 1103 3 0.7645991258557032 1103 4 0.8489062613637373 1103 5 0.762630704006142 1103 6 0.6108668566837423 1103 7 0.6370615640402946 1103 8 0.8756822145687266 1103 9 0.6512394143367999 1103 10-11 0.7519995982255452 1103 12-13 0.7356715831914258 1103 14-15 0.723527507955545 1103 16-17 0.8647042440188102 1103 18-19 0.8721131563883446 1103 20-21 0.8780363222895531 1103 22-23 0.8819837027079487 1103 24-25 0.870107246322668 1103 26-27 0.6012395714755172 1103 28-29 0.7124153427914024 1103 30-31 0.9656041823701216 1103 32-33 0.8944919578551662 1103 34-35 0.9329861022707533 1103 36-37 0.8204989521582107 1103 38-39 0.8709113483459916 1103 40-41 1.027280012138597 1103 42-43 1.0311728763657229 1103 44-45 1.000122606725359 1103 46-47 0.7212237471207779 1103 48-49 0.8534576680156754 1103 50-51 0.7620112205679597 1103 52-53 0.8205339484170366 1103 54-55 0.8587577333381979 1103 56-57 0.8217505650594461 1103 58-59 0.7210775099442621 1103 60-61 0.7720131897585176 1103 62-63 0.7321416361811295 1103 64-65 0.6319620346948938 1103 66-67 0.723972414396389 1103 68-69 0.6188543767126831 1103 70-71 0.689656735522906 1103 72-73 0.631485154199229 1103 74-75 0.7068795606520055 1103 76-77 0.7412530215968829 1104 1 0.623516026198395 1104 2 0.6778177908890939 1104 3 0.5741498128219149 1104 4 0.5244897356661156 1104 5 0.6119662928441443 1104 6 0.4512611574085241 1104 7 0.6174599906882534 1104 8 0.5411705175765249 1104 9 0.530900538513329 1104 10-11 0.44312961981787424 1104 12-13 0.5952673802510162 1104 14-15 0.5196386190666615 1104 16-17 0.6148086986405303 1104 18-19 0.7293141301062178 1104 20-21 0.6782295530249343 1104 22-23 0.9117206063401397 1104 24-25 0.8439178114366115 1104 26-27 0.7197018399080832 1104 28-29 0.7386844767601346 1104 30-31 0.8341775756668639 1104 32-33 0.7498171493667627 1104 34-35 0.9004137267017214 1104 36-37 0.7262121179790668 1104 38-39 0.7874128405548717 1104 40-41 0.827496210512983 1104 42-43 0.7726971299174537 1104 44-45 0.738979038351939 1104 46-47 0.6638664696799559 1104 48-49 0.8016152370217391 1104 50-51 0.6728340671798883 1104 52-53 0.5706903896707693 1104 54-55 0.6920159748697401 1104 56-57 0.6195715274997937 1104 58-59 0.49341367641151024 1104 60-61 0.4377130838598262 1104 62-63 0.45379606260904026 1104 64-65 0.39594925808613013 1104 66-67 0.2231607765424215 1104 68-69 0.3088820203979594 1104 70-71 0.33463693109166215 1104 72-73 0.16621050524652858 1104 74-75 0.3490233903338975 1104 76-77 0.459514013743874 1105 1 -0.07453339248466762 1105 2 0.05167174681151465 1105 3 -0.04443722286636387 1105 4 0.01444549623366953 1105 5 0.15306472558132356 1105 6 -0.16109108558465124 1105 7 -0.07529849977598957 1105 8 0.11235769893122693 1105 9 5.010348581819812E-4 1105 10-11 0.023366058004064882 1105 12-13 0.11823627777199164 1105 14-15 -0.09111196764532536 1105 16-17 -0.07697989621799195 1105 18-19 -0.1057008411651239 1105 20-21 0.024569009743125036 1105 22-23 -0.02276861704201849 1105 24-25 0.04078625307563044 1105 26-27 -0.033504439933302876 1105 28-29 0.14322967381442453 1105 30-31 0.15501488785159978 1105 32-33 0.24635622303620863 1105 34-35 0.252874410254897 1105 36-37 0.2843192637264167 1105 38-39 0.34869011929027494 1105 40-41 0.40330425453661434 1105 42-43 0.1754669499924404 1105 44-45 0.058719806067937697 1105 46-47 0.015348895295662857 1105 48-49 0.08898717195740602 1105 50-51 0.09076209717638406 1105 52-53 -0.12804649360726827 1105 54-55 0.030267168525156762 1105 56-57 0.0845317389858593 1105 58-59 0.0031400123614453435 1105 60-61 -0.14994317035505844 1105 62-63 -0.1652286467324906 1105 64-65 -0.10154271677292925 1105 66-67 0.07767414278557538 1105 68-69 -0.33198550408209115 1105 70-71 -0.5658231320304559 1105 72-73 -0.6140305187344843 1105 74-75 -0.5591290738564396 1105 76-77 -0.25938957666372175 1106 1 0.14673615301146725 1106 2 0.23745476805729737 1106 3 -0.07554893809653151 1106 4 0.07054927794167298 1106 5 -0.11650351421493355 1106 6 0.1192049351318758 1106 7 0.23510905047545094 1106 8 0.3510015241893143 1106 9 0.24022157152283086 1106 10-11 -0.0067134966270003815 1106 12-13 0.06981931646350148 1106 14-15 0.17558481875642684 1106 16-17 0.3129659631791242 1106 18-19 0.29826179930370245 1106 20-21 0.16716878615961406 1106 22-23 0.3104973772329984 1106 24-25 0.3062554172275611 1106 26-27 0.22106195093755332 1106 28-29 0.10122468412399144 1106 30-31 0.020936222631242174 1106 32-33 -0.04919637554683476 1106 34-35 -0.003925675154214048 1106 36-37 0.13758937326520737 1106 38-39 0.1640291629757442 1106 40-41 0.11662756817483455 1106 42-43 0.14733478741329975 1106 44-45 0.06525111498404002 1106 46-47 0.19232824887281197 1106 48-49 0.13679647630657854 1106 50-51 0.027830937672717937 1106 52-53 0.0647477276847539 1106 54-55 0.1191030840215106 1106 56-57 0.04930810463534385 1106 58-59 0.0034813009795016114 1106 60-61 0.0030303886738245467 1106 62-63 -0.1947481722944886 1106 64-65 -0.012354140830790783 1106 66-67 -0.14666111367990808 1106 68-69 -0.3511432877904639 1106 70-71 -0.4707059540418186 1106 72-73 -0.3403058090104203 1106 74-75 -0.5804426885296827 1106 76-77 -0.5360727008178579 1107 1 0.09781607572012518 1107 2 0.22802447892829747 1107 3 0.040975991569276005 1107 4 0.09331320375066099 1107 5 -0.028124931385015373 1107 6 0.00362985967870344 1107 7 -0.02945663036629753 1107 8 0.16290169280524225 1107 9 0.1521276347157965 1107 10-11 0.17713319059485144 1107 12-13 0.26220667704253486 1107 14-15 0.21877733202310168 1107 16-17 0.26671098445906694 1107 18-19 0.2397090006981344 1107 20-21 0.007810964133433629 1107 22-23 -0.05464762677686963 1107 24-25 -0.11172193105041828 1107 26-27 -0.07562930404375123 1107 28-29 0.10658306148137342 1107 30-31 0.20791724778092657 1107 32-33 0.13603838115449918 1107 34-35 0.12199451227265712 1107 36-37 0.24406417834344296 1107 38-39 0.1293335015395698 1107 40-41 0.21816553571432706 1107 42-43 0.22676990078824844 1107 44-45 0.31765263806002153 1107 46-47 0.23686501009228422 1107 48-49 0.2538190840778043 1107 50-51 0.20360321775199708 1107 52-53 0.345161289141668 1107 54-55 0.2133264760817184 1107 56-57 0.1765496597488614 1107 58-59 0.3137607194432839 1107 60-61 0.3000652354484288 1107 62-63 0.3247110416274701 1107 64-65 0.2937474961553015 1107 66-67 0.38980020626067 1107 68-69 0.31688499170142137 1107 70-71 0.35865405518267934 1107 72-73 0.3672099318370847 1107 74-75 0.27586833520839704 1107 76-77 0.16848721988456816 1108 1 -0.049038973866196045 1108 2 -0.002360741322924298 1108 3 -0.028319667471080834 1108 4 -0.1682288112728756 1108 5 0.0287555550746319 1108 6 -0.011324459251035535 1108 7 -0.05658630883763749 1108 8 0.06714191625110288 1108 9 0.001171953981817353 1108 10-11 -0.03952543321212687 1108 12-13 -0.03209933421370437 1108 14-15 0.047559597247492036 1108 16-17 0.027595906846286766 1108 18-19 -0.043701410722370326 1108 20-21 -0.0947186829682991 1108 22-23 -0.013382417856021789 1108 24-25 0.06967889864869647 1108 26-27 -0.1577631835388118 1108 28-29 0.0322737880805235 1108 30-31 -0.0927554682944276 1108 32-33 0.0037288392219494426 1108 34-35 0.21929822333647309 1108 36-37 0.18768442740044833 1108 38-39 0.04904185010156681 1108 40-41 0.13792678060173813 1108 42-43 0.042912092001962776 1108 44-45 0.039773390429857614 1108 46-47 0.06778067862206427 1108 48-49 0.09482125957725884 1108 50-51 0.0155402124601558 1108 52-53 0.07951526791489982 1108 54-55 0.19358866021640608 1108 56-57 0.08543027777430723 1108 58-59 -0.02823580879046972 1108 60-61 -0.029530970449105354 1108 62-63 0.00665703484741087 1108 64-65 -0.019231470252954352 1108 66-67 -0.21034456521370615 1108 68-69 -0.10629450946291286 1108 70-71 -0.228042408520011 1108 72-73 -0.17799863064206178 1108 74-75 -0.26279151490967934 1108 76-77 -0.17635552339005045 1109 1 0.021496607202237783 1109 2 0.015664474782290938 1109 3 -0.07909895391522781 1109 4 0.039731298582090346 1109 5 -0.09819266103573909 1109 6 -0.08487618500788585 1109 7 -0.13199189248455667 1109 8 0.041677917153691624 1109 9 0.12729549583852418 1109 10-11 -0.042780254115250216 1109 12-13 0.12564714690984857 1109 14-15 0.08698769118316818 1109 16-17 0.1997437076452968 1109 18-19 0.0826075292135684 1109 20-21 0.15590553767766124 1109 22-23 0.13887979512723092 1109 24-25 0.09878117374183404 1109 26-27 -0.003312019617403905 1109 28-29 0.1117089071101347 1109 30-31 0.1953677722059055 1109 32-33 0.19907327652987306 1109 34-35 0.20294054527504812 1109 36-37 0.3228247559870496 1109 38-39 0.28910495550280046 1109 40-41 0.3375408428832074 1109 42-43 0.32132918692159507 1109 44-45 0.28902288769810625 1109 46-47 0.10150291161872005 1109 48-49 0.279546879023151 1109 50-51 0.3269535566482169 1109 52-53 0.3782328687248757 1109 54-55 0.32327507752419393 1109 56-57 0.34361741270846835 1109 58-59 0.3405333224846423 1109 60-61 0.23700405559265647 1109 62-63 0.2920534426106087 1109 64-65 0.4722921443127355 1109 66-67 0.6482774471242081 1109 68-69 0.4940593753427578 1109 70-71 0.5686080375046281 1109 72-73 0.5225792312664161 1109 74-75 0.4627090844847075 1109 76-77 0.3757128235473317 1110 1 -0.6267994790952756 1110 2 -0.6998821058501008 1110 3 -0.6111642617498063 1110 4 -0.44667375230960005 1110 5 -0.4648020172866154 1110 6 -0.6721913612841703 1110 7 -0.8280512746920081 1110 8 -0.7995214542071452 1110 9 -0.7223317734089392 1110 10-11 -0.823963713017811 1110 12-13 -0.7194818701750947 1110 14-15 -0.7739531772980612 1110 16-17 -0.8738102814413367 1110 18-19 -0.6789350394529947 1110 20-21 -0.8271717333807231 1110 22-23 -1.0533175255102236 1110 24-25 -0.9484329269403275 1110 26-27 -0.9405874147479878 1110 28-29 -0.877911835709547 1110 30-31 -0.8333507588483613 1110 32-33 -0.5852884130313782 1110 34-35 -0.5504822784737584 1110 36-37 -0.5506150383677451 1110 38-39 -0.586507710832926 1110 40-41 -0.6230976013035345 1110 42-43 -0.5334752534165048 1110 44-45 -0.6782395137574468 1110 46-47 -0.6517576983241291 1110 48-49 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-0.6135775561080479 2314 12-13 -0.5830370178971336 2314 14-15 -0.6520511957481574 2314 16-17 -1.0594158383965109 2314 18-19 -0.9238224439678575 2314 20-21 -0.7979278543824719 2314 22-23 -0.559836106622825 2314 24-25 -0.6882118181930181 2314 26-27 -0.7575724656474776 2314 28-29 -0.8377854306472798 2314 30-31 -1.0113490447035005 2314 32-33 -1.1048703506332345 2314 34-35 -1.1143374307056888 2314 36-37 -1.144956863502415 2314 38-39 -1.0104864650006853 2314 40-41 -0.7372954561536815 2314 42-43 -0.6787264811936602 2314 44-45 -0.6996899431295454 2314 46-47 -0.7337087618015303 2314 48-49 -0.6881301333486292 2314 50-51 -0.7717955624497392 2314 52-53 -0.6432853047736771 2314 54-55 -0.7340083306858176 2314 56-57 -0.6447687502779829 2314 58-59 -0.7611175735884892 2314 60-61 -0.676198953177213 2314 62-63 -0.6942479188724384 2314 64-65 -0.6814234270990589 2314 66-67 -0.7186968623464658 2314 68-69 -0.5816272388613015 2314 70-71 -0.4574290411305597 2314 72-73 -0.33692606882754816 2314 74-75 -0.31093610040684183 2314 76-77 -0.25362256033020003 2315 1 -1.4306441374915906 2315 2 -1.3155264260872102 2315 3 -1.2990561818886377 2315 4 -1.2299518790311623 2315 5 -1.1926052725502245 2315 6 -1.2936764248990258 2315 7 -1.315804231512196 2315 8 -1.3998697410188328 2315 9 -1.290225047842931 2315 10-11 -1.3790926568225572 2315 12-13 -1.271668874252093 2315 14-15 -1.6257231532257705 2315 16-17 -1.591479815851045 2315 18-19 -1.504958896961405 2315 20-21 -1.3893780045767485 2315 22-23 -1.5411662799584462 2315 24-25 -1.4996356979940657 2315 26-27 -1.4735612939280784 2315 28-29 -1.4578785232072207 2315 30-31 -1.4660655649171446 2315 32-33 -1.5042783654839695 2315 34-35 -1.453279964050445 2315 36-37 -1.8226881987349444 2315 38-39 -1.577837211142434 2315 40-41 -1.647105937918134 2315 42-43 -1.7061599787339574 2315 44-45 -1.7026279315330264 2315 46-47 -1.691769026233242 2315 48-49 -1.5389876623807481 2315 50-51 -1.4516491230335369 2315 52-53 -1.524061245596588 2315 54-55 -1.491393435127975 2315 56-57 -1.4620836569235394 2315 58-59 -1.3629688727553493 2315 60-61 -1.381330624850353 2315 62-63 -1.5068896613164675 2315 64-65 -1.4601202775088247 2315 66-67 -1.4115344814543356 2315 68-69 -1.5503663552183298 2315 70-71 -1.7094854119389957 2315 72-73 -2.9417276430104558 2315 74-75 -3.405407014211111 2315 76-77 -3.036744461893093 2316 1 -3.4001532215755503 2316 2 -3.3625559139260943 2316 3 -2.8880575896455767 2316 4 -2.8122157093587745 2316 5 -3.3858443353899617 2316 6 -2.8803077606258825 2316 7 -3.3085307716646284 2316 8 -3.10067009596132 2316 9 -3.0359886714702427 2316 10-11 -2.8690110320043303 2316 12-13 -2.9069927558437314 2316 14-15 -3.0742384140184456 2316 16-17 -4.238553965457385 2316 18-19 -3.92379764789386 2316 20-21 -3.3048271448867155 2316 22-23 -3.5197392629990283 2316 24-25 -3.437791153280024 2316 26-27 -3.112235102035527 2316 28-29 -3.164207413129091 2316 30-31 -3.3418393682527103 2316 32-33 -3.491437960361168 2316 34-35 -3.4880255061289773 2316 36-37 -3.3300885007456955 2316 38-39 -3.401654132386728 2316 40-41 -3.4162191019985926 2316 42-43 -3.7480469452681042 2316 44-45 -3.4677388799665145 2316 46-47 -3.0854300242130748 2316 48-49 -3.3063201858970075 2316 50-51 -3.7143458569923666 2316 52-53 -4.588947699568855 2316 54-55 -3.692713749853315 2316 56-57 -3.543791321845049 2316 58-59 -3.57365689032061 2316 60-61 -3.4240163197685476 2316 62-63 -3.40081281936396 2316 64-65 -3.451244559606188 2316 66-67 -3.5186834801851283 2316 68-69 -3.4398156936548716 2316 70-71 -3.2462439180802853 2316 72-73 -3.2851973097041345 2316 74-75 -3.2217046301173617 2316 76-77 -3.34407391062598 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 77986.0 3 7057.0 4 9466.0 5 10915.0 6 12441.0 7 13034.0 8 15649.0 9 16746.0 10 21044.0 11 23686.0 12 18362.0 13 14654.0 14 14271.0 15 14808.0 16 14931.0 17 15728.0 18 16101.0 19 17352.0 20 18359.0 21 19622.0 22 21874.0 23 24883.0 24 28887.0 25 33949.0 26 39808.0 27 47068.0 28 55194.0 29 64830.0 30 77096.0 31 92739.0 32 113197.0 33 141869.0 34 188801.0 35 252853.0 36 356232.0 37 471459.0 38 412551.0 39 108492.0 40 895.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.629464158146007 26.26520558547163 29.1200496314992 21.98528062488316 2 22.215255986773563 26.55450912829327 29.103979155003675 22.126255729929493 3 22.354845340166936 26.714572120017728 28.525848596870134 22.404733942945203 4 22.028020663621202 26.747125423663455 28.92108187911699 22.303772033598353 5 22.400086761323728 26.74076955916985 28.719822896726484 22.139320782779944 6 22.491278480284873 26.573290545986243 28.787022778030824 22.14840819569806 7 22.356110804106176 26.369566227850456 28.821118266908368 22.453204701135 8 22.29353246335604 26.596122322006828 28.56257490874948 22.547770305887653 9 22.202768791325628 26.585334110093335 28.78016314714506 22.431733951435973 10-11 22.739589149084484 26.61470215004393 28.446768133824126 22.198940567047465 12-13 22.77722019752687 26.329622316370077 28.438911106023074 22.454246380079983 14-15 22.437754764790128 26.554134075134954 28.527476726892143 22.480634433182768 16-17 22.68055912975298 26.398164349582615 28.57841234485234 22.342864175812068 18-19 22.54689199476516 26.455341523013292 28.317748289053768 22.680018193167783 20-21 22.63428089669633 26.618147924146037 28.268132937429208 22.479438241728424 22-23 22.823807539066664 26.73721382289045 27.79187469883003 22.647103939212858 24-25 22.73470363074758 26.869181323216036 27.610177620816895 22.78593742521949 26-27 22.846888737116284 27.055502576700597 27.465116451234305 22.632492234948817 28-29 22.984979667802726 26.910764350496336 27.504661093609357 22.59959488809158 30-31 22.76063875294207 26.951019725930525 27.4637014681912 22.8246400529362 32-33 22.769956203545235 27.22716901933606 27.334155485160817 22.66871929195789 34-35 22.809249378817515 26.850214887653507 27.47862743201077 22.86190830151821 36-37 22.68239557617534 26.975855602881758 27.76517014452003 22.57657867642287 38-39 22.607208286006742 26.855205247923625 27.91207875603855 22.62550771003108 40-41 22.540920886716044 26.926541661107834 27.66803664955264 22.864500802623482 42-43 22.51979076915661 27.158073953824264 27.349969314301603 22.972165962717522 44-45 22.701188554087963 26.808580009850246 27.711473472759728 22.77875796330206 46-47 22.569158460161145 26.904362647200607 27.639900833275945 22.886578059362304 48-49 22.586881926248907 26.89004204062751 27.554868967151013 22.96820706597257 50-51 22.515288979709624 27.17047724620328 27.36862531591256 22.945608458174537 52-53 22.64908088048545 26.993928096248187 27.450160299429932 22.90683072383643 54-55 22.60661733183522 27.01667400686133 27.442399558417563 22.934309102885884 56-57 22.555660400583758 27.190068886110236 27.36558531673786 22.888685396568142 58-59 22.589861897123324 27.1845171632703 27.350143522186286 22.875477417420083 60-61 22.57430085731295 27.139710092631393 27.256209484741113 23.02977956531455 62-63 22.59490678648646 27.068539074748145 27.318131122511698 23.0184230162537 64-65 22.57290600490502 27.048422654685034 27.24415860999753 23.134512730412414 66-67 22.491988840504753 27.120004517791212 27.38031124551234 23.007695396191693 68-69 22.561773792669282 26.898948548041652 27.480551530995466 23.0587261282936 70-71 22.41152967397607 26.815633125820927 27.592078214193517 23.18075898600949 72-73 22.631007656056116 26.63476514083591 27.45522052139322 23.27900668171475 74-75 22.43164596125634 26.67790310433005 27.39503566282694 23.495415271586673 76-77 22.350120102380323 26.61540414885976 27.70413829923039 23.330337449529523 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8394.0 1 5124.5 2 1813.5 3 1828.0 4 1884.0 5 1953.5 6 2108.5 7 2378.5 8 2622.5 9 2682.0 10 2750.5 11 2956.5 12 3270.5 13 3447.0 14 3639.5 15 4055.0 16 4651.5 17 5025.0 18 5345.5 19 6270.5 20 7580.5 21 9683.0 22 11080.0 23 12659.5 24 16182.5 25 21017.0 26 23908.0 27 26403.0 28 33647.0 29 41092.5 30 43789.0 31 49845.5 32 61322.5 33 71744.5 34 81778.5 35 86811.0 36 91871.5 37 100641.0 38 105421.0 39 106492.0 40 109391.0 41 115076.5 42 121514.5 43 125166.0 44 126636.5 45 129595.0 46 131957.5 47 134269.5 48 135707.0 49 136174.5 50 136054.5 51 131811.0 52 128155.0 53 126650.5 54 120070.5 55 108666.0 56 96395.5 57 90454.0 58 85480.0 59 72928.5 60 60091.5 61 54832.0 62 48450.0 63 35915.0 64 25778.0 65 21794.0 66 18590.5 67 13522.5 68 10040.5 69 7002.5 70 5582.0 71 4701.5 72 3259.0 73 2438.5 74 2180.0 75 1955.5 76 1402.5 77 870.0 78 666.0 79 542.0 80 370.5 81 270.5 82 187.0 83 156.0 84 134.5 85 103.5 86 86.5 87 79.0 88 83.0 89 74.0 90 48.5 91 36.0 92 41.5 93 44.0 94 43.0 95 42.0 96 39.0 97 43.0 98 58.0 99 104.0 100 139.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00970777196650199 2 0.013873163484043625 3 0.014389534333325644 4 0.01586979743460077 5 0.01270272289233771 6 0.01005201919935667 7 0.016661566070166535 8 0.01796970555501432 9 0.017797581938586982 10-11 0.0206376216096381 12-13 0.016317318837311856 14-15 0.008571756098081544 16-17 0.006764458125594472 18-19 0.017281211089304964 20-21 0.022393282497196968 22-23 0.020327799100068884 24-25 0.019140146146720237 26-27 0.02377027142861569 28-29 0.008933215692578959 30-31 0.008967640415864427 32-33 0.010878212558207903 34-35 0.011979803703342883 36-37 0.011824892448558276 38-39 0.014664932119609389 40-41 0.012100290234842019 42-43 0.015198515330534144 44-45 0.013546128612831679 46-47 0.023718634343687487 48-49 0.0232194758560482 50-51 0.03442472328546805 52-53 0.03802210686879946 54-55 0.027126681948948825 56-57 0.020740895779494502 58-59 0.015835372711315302 60-61 0.020035188952142405 62-63 0.025818542464101035 64-65 0.0248374378504652 66-67 0.028056149477656462 68-69 0.04096542070970698 70-71 0.05261818954183791 72-73 0.08315291909604808 74-75 0.08354880341383096 76-77 0.08859202537515203 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2904889.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.73605676773337 #Duplication Level Percentage of deduplicated Percentage of total 1 76.14658736707646 41.67963928793466 2 12.644130976235909 13.841797417878096 3 4.500594206727262 7.390343399638661 4 2.175982818709639 4.764188763620131 5 1.2409719783642141 3.3962956327455016 6 0.7296000559898291 2.396125804944044 7 0.49205592337778026 1.885324066843395 8 0.34643683006615256 1.5170068797547624 9 0.24867051577031385 1.2250119120898895 >10 1.3385638292442612 12.981261406640412 >50 0.09188137211599792 3.4211825614024027 >100 0.04206466016366528 4.031514684593087 >500 0.0016396441058312249 0.6060728033787387 >1k 7.567588179570575E-4 0.5613370501959252 >5k 6.306323482975479E-5 0.302898328340256 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8775 0.30207694682998215 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.88494465709361E-5 0.0 2 0.0 0.0 0.0 2.753977862837444E-4 0.0 3 0.0 0.0 0.0 4.1309667942561663E-4 0.0 4 0.0 0.0 0.0 8.950428054221694E-4 0.0 5 3.442472328546805E-5 0.0 0.0 0.0017900856108443387 0.0 6 3.442472328546805E-5 0.0 0.0 0.003304773435404933 0.0 7 3.442472328546805E-5 0.0 0.0 0.007883261632372183 0.0 8 3.442472328546805E-5 0.0 0.0 0.012771572338908646 6.88494465709361E-5 9 3.442472328546805E-5 0.0 0.0 0.017763157215301514 6.88494465709361E-5 10 6.88494465709361E-5 0.0 0.0 0.023925182683400294 1.0327416985640416E-4 11 6.88494465709361E-5 0.0 0.0 0.0313609229130614 1.0327416985640416E-4 12 6.88494465709361E-5 0.0 0.0 0.04096542070970698 1.0327416985640416E-4 13 6.88494465709361E-5 0.0 0.0 0.05507955725674888 1.0327416985640416E-4 14 1.0327416985640416E-4 0.0 0.0 0.0728427144720504 1.0327416985640416E-4 15 1.0327416985640416E-4 0.0 0.0 0.0920172853420561 1.0327416985640416E-4 16 1.0327416985640416E-4 0.0 0.0 0.11229344735719678 1.0327416985640416E-4 17 1.0327416985640416E-4 0.0 0.0 0.13164014184362982 1.0327416985640416E-4 18 1.0327416985640416E-4 0.0 0.0 0.1507458632670646 1.0327416985640416E-4 19 1.0327416985640416E-4 0.0 0.0 0.17177936919448558 1.0327416985640416E-4 20 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.19553242826145853 1.0327416985640416E-4 21 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.22183291685155612 1.0327416985640416E-4 22 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.25136932943048773 1.0327416985640416E-4 23 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.28097459145599024 1.0327416985640416E-4 24 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.3105110040349218 1.0327416985640416E-4 25 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.3418375022246977 1.0327416985640416E-4 26 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.37595240300059657 1.0327416985640416E-4 27 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.40841491705879296 1.0327416985640416E-4 28 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.44108397945670214 1.0327416985640416E-4 29 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.47554312746545563 1.0327416985640416E-4 30 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.5128251027836176 1.0327416985640416E-4 31 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.5504169006113486 1.0327416985640416E-4 32 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.5950313419893153 1.0327416985640416E-4 33 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.6414014442548407 1.0327416985640416E-4 34 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.6920402122077642 1.0327416985640416E-4 35 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.7456739310865235 1.0327416985640416E-4 36 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.8033353425896824 1.0327416985640416E-4 37 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.8576231312108655 1.0327416985640416E-4 38 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.9145616235250297 1.0327416985640416E-4 39 1.0327416985640416E-4 3.442472328546805E-5 0.0 0.9698133043982059 1.0327416985640416E-4 40 1.0327416985640416E-4 3.442472328546805E-5 0.0 1.0321220535449032 1.0327416985640416E-4 41 1.0327416985640416E-4 3.442472328546805E-5 0.0 1.0985617694858565 1.0327416985640416E-4 42 1.376988931418722E-4 3.442472328546805E-5 0.0 1.1701996186429156 1.0327416985640416E-4 43 1.376988931418722E-4 3.442472328546805E-5 0.0 1.2511665678103363 1.0327416985640416E-4 44 1.376988931418722E-4 3.442472328546805E-5 0.0 1.3381578435527142 1.0327416985640416E-4 45 1.376988931418722E-4 3.442472328546805E-5 0.0 1.4273178768620762 1.376988931418722E-4 46 1.7212361642734026E-4 3.442472328546805E-5 0.0 1.5223645378532535 1.7212361642734026E-4 47 1.7212361642734026E-4 3.442472328546805E-5 0.0 1.6161719088061541 1.7212361642734026E-4 48 1.7212361642734026E-4 3.442472328546805E-5 0.0 1.715315111868302 2.4097306299827637E-4 49 2.0654833971280831E-4 3.442472328546805E-5 0.0 1.8179007872589967 2.4097306299827637E-4 50 2.0654833971280831E-4 3.442472328546805E-5 0.0 1.9234469888522419 2.4097306299827637E-4 51 2.0654833971280831E-4 3.442472328546805E-5 0.0 2.0380124679462797 2.4097306299827637E-4 52 2.0654833971280831E-4 3.442472328546805E-5 0.0 2.156984311620857 2.4097306299827637E-4 53 2.0654833971280831E-4 3.442472328546805E-5 0.0 2.285767201431793 2.4097306299827637E-4 54 2.0654833971280831E-4 3.442472328546805E-5 0.0 2.4234660945736652 2.4097306299827637E-4 55 2.0654833971280831E-4 3.442472328546805E-5 0.0 2.56942692130405 2.4097306299827637E-4 56 2.4097306299827637E-4 3.442472328546805E-5 0.0 2.724716848044796 2.4097306299827637E-4 57 2.4097306299827637E-4 3.442472328546805E-5 0.0 2.88017889840197 2.753977862837444E-4 58 2.4097306299827637E-4 3.442472328546805E-5 0.0 3.0453831454489313 2.753977862837444E-4 59 2.4097306299827637E-4 3.442472328546805E-5 0.0 3.2121020803204527 2.753977862837444E-4 60 2.4097306299827637E-4 3.442472328546805E-5 0.0 3.380611100802819 2.753977862837444E-4 61 2.4097306299827637E-4 3.442472328546805E-5 0.0 3.5599639091201074 2.753977862837444E-4 62 2.4097306299827637E-4 3.442472328546805E-5 0.0 3.7408314052619565 2.753977862837444E-4 63 2.4097306299827637E-4 3.442472328546805E-5 0.0 3.934229500679716 2.753977862837444E-4 64 2.4097306299827637E-4 3.442472328546805E-5 0.0 4.1374386422338345 2.753977862837444E-4 65 2.4097306299827637E-4 3.442472328546805E-5 0.0 4.3503555557544535 3.0982250956921247E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATTCGC 385 0.0 23.051405 55 TCGCGCT 405 0.0 22.787233 37 CGATCGT 440 0.0 22.584543 1 CGCGGAA 400 0.0 22.188889 59 CTCGCGC 420 0.0 21.971512 36 CGGTTAC 340 0.0 20.875391 4 CGCGCTT 450 0.0 20.50745 38 CGCTCCG 480 0.0 19.966906 25 AACTCGA 500 0.0 19.877478 13 CAATTCG 490 0.0 19.562786 54 ATTCGCG 470 0.0 18.883835 56 CCTCGCG 530 0.0 18.081367 35 TCGCGGA 540 0.0 17.092781 58 ATCGTTC 540 0.0 17.086601 3 GACGCTC 545 0.0 16.935673 23 CTCCGCG 600 0.0 16.565134 27 GCGGTTA 505 0.0 16.16265 3 ACTCGAG 575 0.0 16.050137 14 CGTAACG 185 8.0248625E-5 15.348348 32 GATCGTT 605 0.0 15.251637 2 >>END_MODULE