FastQCFastQC Report
Thu 26 May 2016
SRR936025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2904889
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107220.3691018830667884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77520.26686045490894833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53500.18417226957725408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA15550.047.65806100-101
CGTACAT20850.041.681576100-101
CGGTATC14150.040.173927100-101
GGTATCA101100.040.0236551
TACGCAG10700.037.272488100-101
CCGTACA9350.033.741283100-101
GTATCAA203750.032.9435581
CCGTATC7600.032.11202100-101
TCGTATC5950.032.01329100-101
CCTGTCG7150.031.62952296-97
CGAGTAC13100.030.898325100-101
GCGTATC4950.030.664248100-101
GCGTACA7200.030.589092100-101
AGTATCA35650.030.471981100-101
TCGTACA11550.030.148884100-101
GGGTATC15350.029.471521100-101
TGTATCA33200.027.43157100-101
AGGTATC23950.027.090582100-101
CGATTTA2200.027.05669100-101
ACGTATC5850.026.964191100-101
Produced by FastQC (version 0.11.5)