FastQCFastQC Report
Thu 26 May 2016
SRR936024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908679
Sequences flagged as poor quality0
Sequence length77
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86810.29845163388603557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG400.002254731735.5084564
AATTCGC3600.025.63925655
CGCGGAA3150.023.66944959
TCGCGCT3350.023.3105637
ATTCGCG4100.020.77971356
CGCTCCG4100.020.7786425
TCGCGGA4000.020.41349458
CTCGCGC4150.019.67092136
CGATCGT4200.019.4340691
ATCGTTC4450.019.1400873
CGCGCTT3950.018.87303738
ACGATCG2657.8216544E-1118.7569259
CAATTCG5000.017.75086454
TAACGCG1250.001601975317.04288152
CGGTTAC3107.2577677E-1016.0278024
CCTCGCG5400.015.77393435
AGCGCGA3652.7284841E-1115.56159923
GATCGTT5250.015.54752352
GAACGAT3703.45608E-1115.35209957
GCGCGAG3703.45608E-1115.35130824
Produced by FastQC (version 0.11.5)