FastQCFastQC Report
Thu 26 May 2016
SRR936024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908679
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106490.3661112140597158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79190.2722541744895191No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54740.18819539729203533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA16050.049.321934100-101
CGGTATC13300.042.738102100-101
GGTATCA103750.041.304081
TACGCAG12200.039.517426100-101
CGTACAT21650.038.076355100-101
CCGTATC8050.034.38116100-101
ACGTATC5850.034.08415100-101
AGTATCA39300.032.410423100-101
CCGTACA8500.032.210865100-101
CCTGTCG7500.032.1372596-97
TCGAGTA5000.031.54564100-101
CGAGTAC14350.031.52283100-101
GTATCAA209150.031.5020141
TCGTATC5850.030.523119100-101
AGGTATC26250.030.496954100-101
GCGTATC4150.029.401485100-101
GCGTACA7050.029.126848100-101
TCGTATA4250.028.709684100-101
GGGTATC15850.028.539598100-101
TCGGTAT4400.028.407309100-101
Produced by FastQC (version 0.11.5)