FastQCFastQC Report
Thu 26 May 2016
SRR936023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181123
Sequences flagged as poor quality0
Sequence length77
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2634014.542603645036797No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2580214.24556792897644No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7890.4356155761554303No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3090.17060229788596698No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2280.12588130717799506No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAA200.006546026653.2866964
GGTCTCC200.006574708553.2278446
ACAAGGT305.444075E-447.37904469
GCCGTAT500.006717783428.39602527
CCGGGGG850.003483292220.8794336
ACACTCT1000.00889515417.75241761
CGGGGGG1401.6658199E-417.74751537
CCCCGGG2203.464605E-412.90728434
CCCCGCC2100.0035802911.83494561
CGCAAAA4551.8121973E-610.1386371
AAAAGAG3903.749221E-510.0224971
AAAAGGG3200.0077838318.88357171
GCAAAAA5602.5230327E-58.2376422
CTCTCTT4450.00154891657.978614351
TTAAACA4904.526189E-47.97047360
GCAGTGG5904.845275E-57.81877951
CCCCCCG4950.00441058177.170712532
TGGTATC7903.4511177E-56.7377025
GTGGTAT7401.07340544E-46.71342134
GGTATCA8004.1113533E-56.65348056
Produced by FastQC (version 0.11.5)