FastQCFastQC Report
Thu 26 May 2016
SRR936022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180201
Sequences flagged as poor quality0
Sequence length77
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2623314.557632865522388No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2572514.275725439925417No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8080.44838818874479053No Hit
TGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.1442833280614425No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.1254155082380231No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG1950.10821249604608188No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC200.006553688653.2708362
TTGTGGC200.006568102653.2412634
GTATCAT556.0560433E-638.72091730
TCGCCGT707.7476216E-735.4941725
CGTATCA601.0999629E-535.4941729
CCGTATC651.9027557E-532.7638528
GCCGTAT651.9027557E-532.7638527
CGCCGTA651.906374E-532.7547626
ATCATTA853.5277371E-629.23049432
CGCCGGA500.006709383328.40322168
TGTGGCT500.00671848928.39533635
TGGTCGC905.503942E-627.60657522
TCATTAA1055.948659E-727.04317933
CATTAAA1055.948659E-727.04317934
GTCGCCG1108.928364E-725.81394424
GGTCGCC1108.928364E-725.81394423
TATCATT901.7460415E-423.66277931
GTGGTCG1102.607587E-522.587221
AAGTTGA800.002450654522.1838591
ATTAAAA1459.822648E-619.5829935